Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,352,815	G→A	A92V (GCA→GTA)	rpsI ←	30S ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,352,815	0	G	A	98.6%	202.7 / NA	70	A92V (GCA→GTA)	rpsI	30S ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (39/29);  minor base C (1/0);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTA  >  USA300TCH1516_ALE/2352678‑2352941                                                                                                                                          |                                                                                                                               tatCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACtt                                                                                                                             <  1:799391/141‑1 (MQ=255)   tCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCt                                                                                                                           >  1:745435/1‑141 (MQ=255)        cGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCtaataa                                                                                                                      >  1:603050/1‑141 (MQ=255)          aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCGGGATCTACTTCTAATAATg                                                                                                                    >  1:417476/1‑141 (MQ=255)          aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTCGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCACCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTGTAATCATg                                                                                                                    >  2:680809/1‑141 (MQ=38)            aaTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGcc                                                                                                                  >  2:401017/1‑140 (MQ=255)              ttAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCCCTGTATTCAGGATCTACTTCTAATAATGCACg                                                                                                                >  1:421343/1‑141 (MQ=255)               tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGCGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCCGGATCTACTTCTAATAATGCACgc                                                                                                               >  2:341639/1‑140 (MQ=255)               tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCGGGATCTACTTCTGATAATGCACGa                                                                                                               >  1:226781/1‑141 (MQ=255)                aaCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAg                                                                                                              >  2:546263/1‑141 (MQ=255)                 aCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGGACGAGc                                                                                                             >  1:541267/1‑141 (MQ=255)                  cGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTCCTTCTAATAATGCACGAGCg                                                                                                            >  1:458844/1‑141 (MQ=255)                   gtctggagaAGTGAGGTGGACGACCAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGa                                                                                                           <  1:776960/136‑1 (MQ=255)                        gagaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATTCACGAGCGATTCCg                                                                                                      <  1:493443/141‑1 (MQ=255)                        gagaATTGAGGCGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCg                                                                                                      <  1:41872/141‑1 (MQ=255)                        gacaaTTGAGGTTAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCg                                                                                                      <  2:469746/138‑1 (MQ=255)                         agaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGt                                                                                                     >  1:780536/1‑141 (MQ=255)                         agaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGt                                                                                                     >  1:561189/1‑141 (MQ=255)                         agaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTGTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAGTCCGGCGCGATTTAAAGAACCTCTGTATTCAGGGGCAACTTCTAATAATGCACGAGCGATTCCgg                                                                                                     >  1:154843/1‑140 (MQ=255)                          gaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAGCGTCTGGATTCAGAAACTACTTCTAATAATGCACGAGCGATTCCGTg                                                                                                    >  1:711120/1‑141 (MQ=255)                          gaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCGGGATCTACTTCTAATACTGCACGAGCGATTGCGTg                                                                                                    >  1:507578/1‑141 (MQ=255)                          gaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTg                                                                                                    >  1:460198/1‑141 (MQ=255)                               gaagTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGa                                                                                               <  2:249493/138‑1 (MQ=255)                                 ggTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATa                                                                                             <  2:372427/141‑1 (MQ=255)                                 ggTGAACGACGAGCTGCTTCAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCACGACCTACTTCGAATAATGCACGAGCGATTCCGTGACGGATa                                                                                             <  1:442532/141‑1 (MQ=255)                                     aaCGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagctt                                                                                         <  1:791541/141‑1 (MQ=255)                                         acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagc                                                                                     >  1:60607/1‑141 (MQ=255)                                         acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGACCTACTTCTAATAATGCACGAGCGATTCCGGGACGGATagcttgagc                                                                                     >  2:28241/1‑141 (MQ=255)                                          cgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGGTTCCGTGACGGATagcttgggct                                                                                    >  1:100242/1‑141 (MQ=255)                                            aGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagcttg                                                                                  >  1:402942/1‑141 (MQ=255)                                            aGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagcttg                                                                                  >  1:846283/1‑141 (MQ=255)                                                    tAAGACCTGGTTTTTGACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTGCGAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGa                                                                          <  1:127846/141‑1 (MQ=255)                                                      aGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaaa                                                                        <  2:143065/141‑1 (MQ=255)                                                       gACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAac                                                                       <  2:603050/141‑1 (MQ=255)                                                           tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                   <  1:605705/141‑1 (MQ=255)                                                           tGGTTTTTTACGTTCTTTCATACAGGGGTCACGAGCAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                   <  2:709994/141‑1 (MQ=255)                                                              ttttttACGTTCTTTCATACGGGGGTCACGAGTAAGTAATCCAGCGCGTTTAAAAGAACCCCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccacc                                                                <  1:714140/141‑1 (MQ=255)                                                               tttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAAccaccacca                                                               >  1:248922/1‑141 (MQ=255)                                                               tttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGGATTCGGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGGGCTTGTCCAGTGAAGccaccacca                                                               >  1:151070/1‑141 (MQ=255)                                                               tttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCAAGCGCGTATTAAAGATCCGCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCGGTGAAAccaccacca                                                               >  2:331250/1‑141 (MQ=255)                                                                ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccaccct                                                              >  1:359794/1‑139 (MQ=255)                                                                ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCAt                                                              >  1:757416/1‑141 (MQ=255)                                                                      gTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGt                                                        >  1:625726/1‑141 (MQ=255)                                                                       ttCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGtt                                                       <  2:15241/141‑1 (MQ=255)                                                                       ttCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAACAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGtt                                                       <  2:429195/141‑1 (MQ=255)                                                                          tttCCTACGTTGGTCACGAGTAAGTAACCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCTATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAAc                                                    <  1:282065/141‑1 (MQ=255)                                                                          tttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAAc                                                    <  1:594592/141‑1 (MQ=255)                                                                          tttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAAc                                                    <  1:594720/141‑1 (MQ=255)                                                                             cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTAGTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                 <  2:405833/141‑1 (MQ=255)                                                                              aTACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGAAAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACGaaa                                                >  2:411596/1‑141 (MQ=255)                                                                                  gTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcat                                            >  2:846036/1‑141 (MQ=255)                                                                                     ggTCACGGGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatcat                                         <  2:783053/141‑1 (MQ=255)                                                                                      gTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATa                                        <  1:661011/141‑1 (MQ=255)                                                                                      gTCAAGAGTCAGTAATCCAGCGCTTTTTAAAGAACCACTGTATTCAGGATCTAATTCTAATAATGCACGAGCTATTCCTTGATGGATAGCTTGAGCTTGTCCAGTGATACGACCACCATGAACGTTAACTAAAACATCATa                                        <  2:344059/141‑1 (MQ=255)                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCCTGCACGTTAACTAAAACATCGTAGTTAcat                                 >  2:153125/1‑139 (MQ=255)                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCAACATGAACGTTACCTAAAACATCCTAGTTACCt                                 >  1:392123/1‑141 (MQ=255)                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGAGTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGGTAACTAAAACATCATAGTTACCt                                 >  2:888317/1‑141 (MQ=255)                                                                                                agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTa                              >  1:513502/1‑141 (MQ=255)                                                                                                 gtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAg                             >  1:833139/1‑141 (MQ=255)                                                                                                 gtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAg                             >  1:378639/1‑141 (MQ=255)                                                                                                         gcgcGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACCTCATAGTTACCTTTAGTTTCAGtt                     >  2:300802/1‑141 (MQ=255)                                                                                                              ttttAAAGAACCTCTGTATTCATGATCTACTTCTAATAATGCACGAGCGATTCCGTGCCGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                >  2:464085/1‑141 (MQ=255)                                                                                                                 tAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaaa             <  1:604737/141‑1 (MQ=255)                                                                                                                 gagaGAACCTATGTATTCAGGATCTACTGCTAATAATGCACGAGCGAATCCGTGACGGATAGCTTGAGCTTGTCCAGTTAAACCTCCACCATGAACGTCAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaaa             <  1:123291/138‑1 (MQ=255)                                                                                                                   aaGGACCTCTGTATTCAGGATCTACTTGTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtg           <  2:421343/141‑1 (MQ=255)                                                                                                                     gAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACCTTAACTAAAACATCATAGCTACCTTTAGTTTCAGTTACATCAAAtggt         >  2:184794/1‑141 (MQ=255)                                                                                                                        ccTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGGGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCCGTACTATCAAAttgttgg      <  2:427105/141‑1 (MQ=255)                                                                                                                            tGTATTCAGTATCTACTTCTAATAATGCACGCGCTATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACTTCAATTGGTTGGTTTa  >  1:677108/1‑141 (MQ=255)                                                                                                                            tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCCCCGCCATGAACGTTAACTCAAACATCATAGTTACCTTTCGTTTCAGTTACCTCACAAGGTGGGTTTa  >  2:124883/1‑141 (MQ=255)                                                                                                                            tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTGGAGCGTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTa  <  1:714932/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                               TATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTA  >  USA300TCH1516_ALE/2352678‑2352941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑