Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 195,286 T→A intergenic (‑97/‑82) USA300HOU_RS00900 ← / → USA300HOU_RS00905 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE195,2860TA100.0% 285.6 / NA 82intergenic (‑97/‑82)USA300HOU_RS00900/USA300HOU_RS00905hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (35/47);  total (35/47)

ACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAA  >  USA300TCH1516_ALE/195163‑195421
                                                                                                                           |                                                                                                                                       
aCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAata                                                                                                                        >  2:243132/1‑141 (MQ=255)
    gCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAGTATCTTAATATCCt                                                                                                                    >  2:142822/1‑141 (MQ=255)
       aCGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGAATTTTATACTCTTTGAAAATATATTTTACATAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGt                                                                                                                 <  1:182389/141‑1 (MQ=255)
          aCAGATTCTTTATCCATAATGATAGCCCGCTATATATATGTTTATTTACTTATAGCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAAt                                                                                                              >  1:597534/1‑141 (MQ=255)
          aCAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAAt                                                                                                              >  1:595966/1‑141 (MQ=255)
               ttCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGAt                                                                                                         >  2:438859/1‑141 (MQ=255)
               ttCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGAt                                                                                                         >  1:820974/1‑141 (MQ=255)
                tCTTTATCCATAATGATAGCCTCCTAGATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                        <  2:733663/141‑1 (MQ=255)
                tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                        <  2:372563/141‑1 (MQ=255)
                tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                        <  1:659877/141‑1 (MQ=255)
                 cTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATaa                                                                                                       >  1:157114/1‑141 (MQ=255)
                 cTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATaa                                                                                                       >  1:778853/1‑141 (MQ=255)
                       ccATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATta                                                                                                 >  2:858146/1‑141 (MQ=255)
                           aaTGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGt                                                                                             <  1:67210/141‑1 (MQ=255)
                               aTAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAata                                                                                         >  2:702951/1‑141 (MQ=255)
                                tAGCCCCCGATATATATGTTTATTTACTTATATCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAatat                                                                                        <  2:815516/141‑1 (MQ=255)
                                 aGCCTCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtt                                                                                       <  1:125907/141‑1 (MQ=255)
                                 aGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtt                                                                                       <  1:447487/141‑1 (MQ=255)
                                  gCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATGTTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTTTAATCACATCAGAATTATATTAATAttt                                                                                      >  2:816004/1‑141 (MQ=255)
                                   caaccTATATAAATGTTTATTTACTTATACCCTAACATTATTTTTATACTCTTTGAAAATATATTTTACACTCTTTTATCTAAAGAGTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtttt                                                                                     <  2:626/138‑1 (MQ=255)
                                     cccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtttttt                                                                                   >  1:47396/1‑141 (MQ=255)
                                      ccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTc                                                                                  >  2:728175/1‑141 (MQ=255)
                                       cTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCa                                                                                 <  1:645609/141‑1 (MQ=255)
                                       cTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCa                                                                                 <  1:291952/141‑1 (MQ=255)
                                       cTATATATATGTTTATTTACTTATACCATAACATGATTTTTCATCTCCTTGAAAATATATTTTCCAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCa                                                                                 <  1:623971/141‑1 (MQ=255)
                                         ttatatatGTTTATATACTTATACCCTAACATGATTTTTATCCTCTTTGAAAATATATTTTACATAATTTTAAAAAAAAAAAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAAc                                                                               <  2:637333/140‑1 (MQ=255)
                                         atatatatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTAGAAAATATATTTTACAGAATTTTATCAAAAAATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAAc                                                                               <  2:448318/141‑1 (MQ=255)
                                             atatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCAtt                                                                           <  1:862529/141‑1 (MQ=255)
                                              tatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTg                                                                          <  1:202946/141‑1 (MQ=255)
                                              tatGTTTATTTACTCATCCCCTAACATGATTTTTATAATCTTGTAAAATATATTTTACAGAATTTTAACGAAATATTAAAAAAAATATCTTAATTTCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTg                                                                          <  2:67899/141‑1 (MQ=255)
                                              taattttatttaCTTATACCCTAACATGAATTTTATAATCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTg                                                                          <  2:203259/137‑1 (MQ=255)
                                                  tttatttaCTTATACCCTAACTTGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTtat                                                                      <  1:568979/141‑1 (MQ=255)
                                                   ttatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTtata                                                                     <  1:183635/141‑1 (MQ=255)
                                                    tatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTTATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTtatcg                                                                    >  1:73514/1‑139 (MQ=255)
                                                     atttaCTTATACCCTAACATTATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                   <  1:420439/141‑1 (MQ=255)
                                                     atttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                   <  2:820974/141‑1 (MQ=255)
                                                     atttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                   <  1:438859/141‑1 (MQ=255)
                                                     atttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                   <  1:158494/141‑1 (MQ=255)
                                                          cTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTTTAATCCAATAAGAATTATAGTAATATTTTATCAACCATTGTTATAGGAGGtt                                                              >  1:286258/1‑140 (MQ=255)
                                                          cTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTc                                                              >  1:388447/1‑141 (MQ=255)
                                                            tataCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtt                                                            <  2:78762/141‑1 (MQ=255)
                                                              taaccTAACTTGATTTTTATAGTCTTTGATATTATATTTTACAGAATTTTATCTAAATATAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCttat                                                          <  2:551769/138‑1 (MQ=255)
                                                               agccTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCttatt                                                         <  2:685261/139‑1 (MQ=255)
                                                               aCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCttatt                                                         <  1:392516/141‑1 (MQ=255)
                                                                    aaCATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGa                                                    <  1:615960/141‑1 (MQ=255)
                                                                    aaCATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGa                                                    <  1:615865/141‑1 (MQ=255)
                                                                     aCATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGaa                                                   >  1:61177/1‑140 (MQ=255)
                                                                     aCATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGAc                                                   >  1:494298/1‑141 (MQ=255)
                                                                      cATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTATTCCGATAAGAATTATAGTAATATTTTTTCAACCATTTTTATAGGAGGTCGTATTAATAACa                                                  >  2:242023/1‑141 (MQ=255)
                                                                      cATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTCTATCTAAATATTAAAAAAAAGATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTCATGCCa                                                  >  2:560313/1‑141 (MQ=255)
                                                                       aTGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGGAGTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACat                                                 >  1:863191/1‑141 (MQ=255)
                                                                       aTGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACat                                                 >  2:332944/1‑141 (MQ=255)
                                                                       aTGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACat                                                 >  2:731350/1‑141 (MQ=255)
                                                                       aTGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACat                                                 >  2:593987/1‑141 (MQ=255)
                                                                           tttttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACattatt                                             <  1:435014/141‑1 (MQ=255)
                                                                           tttttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACattatt                                             <  2:3564/141‑1 (MQ=255)
                                                                                ataCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATGGTGATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttat                                        <  1:629196/141‑1 (MQ=255)
                                                                                 taCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttatt                                       >  2:810501/1‑141 (MQ=255)
                                                                                 taCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttatt                                       <  1:341746/141‑1 (MQ=255)
                                                                                 aaCTCTTTGAAAATATATTTTACAGAATTTTATAAAAATAATAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTGATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTatatt                                       <  2:562632/140‑1 (MQ=255)
                                                                                  aCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAAAATATTAATATCTTTTTAATCCGGTAAGGATTATATTATTATTTTTTCAACCTTTGTTAAAGAGGTTCTTATTATAAAATTTATTTttttta                                      >  2:639052/1‑141 (MQ=255)
                                                                                       ttGAAAATATATTTTACAGAATTTTATCTAAATACTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAAAGACATTATTTTTATTAGAAGc                                 <  1:478376/141‑1 (MQ=255)
                                                                                        tGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCt                                <  1:244872/141‑1 (MQ=255)
                                                                                           aaaTATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTaaa                             >  1:873778/1‑140 (MQ=255)
                                                                                           aaaTATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAAc                             >  1:876737/1‑141 (MQ=255)
                                                                                              tatatTTTACAGAATTTTATTTAAATATTAAAAAAAATATCTTAATATTCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAAt                          <  2:146649/141‑1 (MQ=255)
                                                                                               atatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATc                         <  1:731350/141‑1 (MQ=255)
                                                                                                tatTTTACTGAATTTTATTTAAATATAAAAAAAGATATCTTAATATCCTGGTAATCCGATAAGAATGATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGTCATTATTTTTTTTAGAAGCTAACATTca                        <  2:438634/141‑2 (MQ=255)
                                                                                                tatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCt                        <  2:671209/141‑1 (MQ=255)
                                                                                                 atTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCtt                       <  2:761164/141‑1 (MQ=255)
                                                                                                  ttttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTCACAATCTTg                      >  1:895271/1‑141 (MQ=255)
                                                                                                    ttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCGAAAAATCTTGAt                    >  2:40590/1‑141 (MQ=255)
                                                                                                    ttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTGCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAt                    >  1:622837/1‑141 (MQ=255)
                                                                                                    ttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTGCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAt                    >  1:623094/1‑141 (MQ=255)
                                                                                                    ttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTGAAACCCTTGTTATAGGAGGAATTATTAATTACATTAGCTTTATTAGAATCTAACAAGATTGAt                    >  2:834759/1‑141 (MQ=255)
                                                                                                     tACAGAATTTTGTGTAAATATTAGAAAAAATATCTTAATATCCTTGTAATCCGATAATAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAtt                   <  2:203190/141‑1 (MQ=255)
                                                                                                       cAGAATTTTATATAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAgttt                 <  2:336085/141‑1 (MQ=255)
                                                                                                            ttttATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTTTAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATc            >  2:713796/1‑141 (MQ=255)
                                                                                                                 tCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGa       <  2:45278/141‑1 (MQ=255)
                                                                                                                 accaaaTATTAAAAAAACTATCTTAATATCCTTGTAATCCGATAAGACTTATAGTAATATTTTTTCACCCATTGTTATAGGAGGTCTTATTCATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGa       <  1:464431/138‑1 (MQ=255)
                                                                                                                  cTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGaa      >  2:267378/1‑141 (MQ=255)
                                                                                                                      atatTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAaagaa  <  1:243132/141‑1 (MQ=255)
                                                                                                                           |                                                                                                                                       
ACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAA  >  USA300TCH1516_ALE/195163‑195421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑