Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 982,331 T→C 5.5% D67D (GAT→GAC)  USA300HOU_RS04855 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE982,3310TC 5.5% 217.0 / 4.3 73D67D (GAT→GAC) USA300HOU_RS04855hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (41/28);  new base C (2/2);  total (43/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

CCGTTTTTATTAATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCGGGTGTTGATACCGC  >  USA300TCH1516_ALE/982194‑982471
                                                                                                                                         |                                                                                                                                            
ccGTTTTTATTAATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCt                                                                                                                                           <  1:561260/141‑1 (MQ=255)
    ttttATTAATTTACTTTTTGATTCAAGGTTATTCCATAATAAAATTAGAAATATTAATGGCGTCGTATGGCATTGCAGCATTTTTATTCTCTCTATAAAAAGAAAAATGTTTTAAAATTTGTAACTAACAAAATACtcata                                                                                                                                       >  1:441506/1‑141 (MQ=255)
        attaATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAAtt                                                                                                                                   <  1:682867/141‑1 (MQ=255)
         ttaATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGACTCTAATAAATTa                                                                                                                                  >  1:684896/1‑141 (MQ=255)
         ttaATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGACTCTAATAAATTa                                                                                                                                  >  1:685028/1‑141 (MQ=255)
          taATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAg                                                                                                                                 >  1:86447/1‑141 (MQ=255)
          taATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAg                                                                                                                                 >  1:439782/1‑141 (MQ=255)
             tttACTTTTTGAGTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAgttg                                                                                                                              <  1:744704/141‑1 (MQ=255)
                ttcttttGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGGATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTa                                                                                                                           <  2:297495/138‑1 (MQ=255)
                aCTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTa                                                                                                                           <  1:326751/141‑1 (MQ=255)
                aCTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTa                                                                                                                           <  2:379302/141‑1 (MQ=255)
                 cTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAg                                                                                                                          >  2:232668/1‑141 (MQ=255)
                     ttGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGaa                                                                                                                      >  2:627491/1‑141 (MQ=255)
                           cAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTc                                                                                                                >  2:145534/1‑141 (MQ=255)
                           cAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTATCTATACAAAGAGAAGTGGTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTc                                                                                                                >  2:280411/1‑141 (MQ=255)
                            aaGGTTATTCCATAATACACTTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTACCTTAAAATATTGTAATAAATTAGATGTTATTGGAATAATCa                                                                                                               >  1:504874/1‑141 (MQ=255)
                            aaGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCa                                                                                                               >  2:726824/1‑141 (MQ=255)
                               gTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCaaaa                                                                                                            <  2:155312/141‑1 (MQ=255)
                               gTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCaaaa                                                                                                            <  1:82665/141‑1 (MQ=255)
                                 tATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTTCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAatc                                                                                                          <  2:768077/141‑1 (MQ=255)
                                 tATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAatc                                                                                                          <  2:607712/141‑1 (MQ=255)
                                 tATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAatc                                                                                                          >  2:690330/1‑141 (MQ=255)
                                  aTTCCATAATACAATTAGAGATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTAAAAatca                                                                                                         >  1:420535/1‑141 (MQ=255)
                                  aTTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAatca                                                                                                         >  1:386929/1‑141 (MQ=255)
                                  aTTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAatca                                                                                                         >  1:275995/1‑141 (MQ=255)
                                      cATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAGAATCATCgg                                                                                                     <  1:579305/141‑1 (MQ=255)
                                       ataataCAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAATATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGt                                                                                                    <  2:477203/141‑1 (MQ=255)
                                       ataataCAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGt                                                                                                    >  1:779335/1‑141 (MQ=255)
                                         aataCAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGttt                                                                                                  <  1:103654/141‑1 (MQ=255)
                                             cAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAGTAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTAttg                                                                                              >  1:736634/1‑141 (MQ=255)
                                             cAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTAttg                                                                                              >  1:560780/1‑141 (MQ=255)
                                               aTTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTAttgtt                                                                                            >  1:620552/1‑141 (MQ=255)
                                               aTTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTAttgtt                                                                                            <  1:481209/141‑1 (MQ=255)
                                                   gAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGtta                                                                                        <  2:377706/141‑1 (MQ=255)
                                                     aaTATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGttatt                                                                                      >  1:682908/1‑141 (MQ=255)
                                                     aaTATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTATATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGttatt                                                                                      >  2:167686/1‑141 (MQ=255)
                                                      atatTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGttatta                                                                                     <  2:424818/141‑1 (MQ=255)
                                                         ttAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTatat                                                                                  >  1:154520/1‑141 (MQ=255)
                                                         ttAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGATGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTatat                                                                                  >  2:570543/1‑141 (MQ=255)
                                                          tAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATc                                                                                 >  1:636525/1‑141 (MQ=255)
                                                            aTGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCaa                                                                               >  2:30865/1‑141 (MQ=255)
                                                            aTGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCaa                                                                               >  2:50091/1‑141 (MQ=255)
                                                             tGGCGCCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCaaa                                                                              <  2:573981/141‑1 (MQ=255)
                                                                   tttATGGCATTGCAGCATTTTTATTCTCTCTATAAAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAat                                                                        <  2:302797/140‑1 (MQ=255)
                                                                     tATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAatat                                                                      <  1:558764/141‑1 (MQ=255)
                                                                        ggCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTa                                                                   >  2:471229/1‑141 (MQ=255)
                                                                           aTTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAGtt                                                                >  1:695795/1‑141 (MQ=255)
                                                                               cagcaTTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtag                                                            >  1:696128/1‑141 (MQ=255)
                                                                               cagcaTTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtag                                                            >  2:408119/1‑141 (MQ=255)
                                                                               cagcaTTTTTATTCTCTCTATAAAAAGCGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtag                                                            >  2:545111/1‑141 (MQ=255)
                                                                               cagactttttATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAATATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtag                                                            <  2:455958/136‑1 (MQ=255)
                                                                                 gcaTTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAACGATTCTAATAAATTATTTGTTATTGAAATATTCAAAATCATCTGTTTATTGTTGTTATTATATCAAAATACAAATTTAATTttatta                                                          >  2:677908/1‑141 (MQ=255)
                                                                                 gcaTTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtagta                                                          >  1:646714/1‑141 (MQ=255)
                                                                                 gcaTTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTtagta                                                          >  2:460914/1‑141 (MQ=255)
                                                                                    tttttATTTTCTCTATACTAAGATAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAAGTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTAGTTATTATATCAAAATCAATATTTAATTTTAGTAGTg                                                       <  2:109683/141‑1 (MQ=255)
                                                                                      tttATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGc                                                     >  2:652845/1‑141 (MQ=255)
                                                                                         aTTCTCTCTATACAAAGAGCAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCAACGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGcaca                                                  >  1:231537/1‑141 (MQ=255)
                                                                                         aTTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGcaca                                                  >  1:692739/1‑141 (MQ=255)
                                                                                         aTTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATAAAAATCAATATTTAATTTTAGTAGTGGcaca                                                  >  2:153015/1‑141 (MQ=255)
                                                                                                  ataCAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTCGCACAAATattatt                                         <  2:536592/141‑1 (MQ=255)
                                                                                                     cAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAgg                                      <  2:779335/141‑1 (MQ=255)
                                                                                                     cAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGACTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAgg                                      <  2:685028/141‑1 (MQ=255)
                                                                                                     cAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGACTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAgg                                      <  2:684896/141‑1 (MQ=255)
                                                                                                      aaaGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAggg                                     >  2:233812/1‑141 (MQ=255)
                                                                                                         aagaAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTa                                  <  2:45562/140‑1 (MQ=255)
                                                                                                             aGTGATTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTGTAGTAGTGGCACAAATATTATTAGGGTTAAGtt                              >  1:536056/1‑141 (MQ=255)
                                                                                                              gtgtTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTa                             >  1:620349/1‑141 (MQ=255)
                                                                                                                      aaaTTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGGATAATATTCAAAACCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAatga                     <  1:339999/141‑1 (MQ=255)
                                                                                                                       aaTTTGTAACTTAAAAGATTCTAATAAATTAGTTGTCACTGAAAGATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATCAGGGTTAAGTTACTCAatgat                    <  1:410067/141‑1 (MQ=255)
                                                                                                                          ttGTGACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGc                 >  1:556809/1‑141 (MQ=255)
                                                                                                                           tGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCg                >  1:675397/1‑141 (MQ=255)
                                                                                                                              aaCTTAAAAGATTCTAATAAATTAGTTGGTAGTGAAATATTCAAAATCATCTGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCGGgt             <  1:167686/141‑1 (MQ=255)
                                                                                                                                cTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCGGgtgt           <  1:331713/141‑1 (MQ=255)
                                                                                                                                         ttCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCGGGTGTTGATACCGc  <  1:460914/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
CCGTTTTTATTAATTTACTTTTTGATTCAAGGTTATTCCATAATACAATTAGAAATATTAATGGCGTCTTATGGCATTGCAGCATTTTTATTCTCTCTATACAAAGAGAAGTGTTTTAAAATTTGTAACTTAAAAGATTCTAATAAATTAGTTGTTAGTGAAATATTCAAAATCATCGGTTTATTGTTGTTATTATATCAAAATCAATATTTAATTTTAGTAGTGGCACAAATATTATTAGGGTTAAGTTACTCAATGATGGCGGGTGTTGATACCGC  >  USA300TCH1516_ALE/982194‑982471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑