| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,443 | C→A | 59.3% | V77F (GTT→TTT) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,443 | 0 | C | A | 59.3% | 10.4 / 42.5 | 45 | V77F (GTT→TTT) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/13); new base A (15/11); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
AGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTC > USA300TCH1516_ALE/1744303‑1744581 | aGTAGCTAATAAAACATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAc < 2:618816/141‑1 (MQ=255) tacatcttcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTCCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCaaa < 1:253768/138‑1 (MQ=255) aaTCATCTGTACTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGc < 2:29936/141‑1 (MQ=255) aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAACACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGc > 1:475816/1‑141 (MQ=255) gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGCATGACTTCGCGAGGTAATTTCCCTTCTTTTCTAAAAGGTTCAAACACGATcccc > 2:18833/1‑141 (MQ=255) gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAAGATTTTATTTGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATcccc < 1:615363/141‑1 (MQ=255) acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGaaa > 2:108255/1‑140 (MQ=255) acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAAt < 1:93836/141‑1 (MQ=255) cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCAATTGAATa > 1:253365/1‑141 (MQ=255) acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATaa > 1:190855/1‑141 (MQ=255) ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc < 1:745407/141‑1 (MQ=255) tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACa > 1:461154/1‑141 (MQ=255) gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg < 2:202012/141‑1 (MQ=255) gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTACGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAGTCCCCATTGAATAAGCTACAg > 1:103733/1‑141 (MQ=255) aCCTGGTTTAATTGGATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAgg > 1:414526/1‑141 (MQ=255) cttgTTTAATTGCATCTTTGTGCATTTTTAAAACATTTGTACCATATTCTAGGTCATCCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCCAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc < 2:99404/138‑1 (MQ=255) tttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGcc < 2:426664/141‑1 (MQ=255) aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATg < 1:76821/141‑1 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa > 1:122534/1‑141 (MQ=255) tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGTTGCAAAGCCAATACCCATTGAATAACCTACCGGTAAGCCAATGaa > 1:181202/1‑140 (MQ=255) tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAACATCCCTACTTTTCTAACGGCTGCAAGGCCAATCCCCATTGAATAAGCTACAGGACAGCCAGTGAt > 2:675461/1‑141 (MQ=255) tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGcc < 2:9847/141‑1 (MQ=255) ctttGTTAAAACATTTTTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTACTTTCCTTTCTTTTCTAAAAGGTGCAAAGCCAATCTCCATTGAAGAAGCTACAGGACAGCCAATGATAAAGCCACgcgc < 2:98866/139‑1 (MQ=255) aTTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCt < 2:37927/141‑1 (MQ=255) aTTGTTAAAACATTTGTACCATACTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCt < 2:6851/141‑1 (MQ=255) tgtgAAAACATTTGTACCATATTCTAGGTCGTACTCATAACGAATGACTGCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTc < 2:630859/141‑1 (MQ=255) ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg < 2:780618/141‑1 (MQ=255) tAAAACATTTGTACCATATTCTAGTTCATACTCATAACGAATGACTGCACGAGGTAATTTCCCTTCTTTTCTACAAGGTGCAAAGCCAATCCCCATTGAATAAGCGACAGGAAGGCCAATGATAAGGCGACGGGCtccgcg > 1:171835/1‑136 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTTATTTCCCTTCTTTTCTAAAAGGGGCAAAGCCAATCGCCATTGAGTAAGCGACAGGACAGGCAAAGATAAAGCCACGAGCttacgg > 1:263179/1‑137 (MQ=255) ttGTTCCATATTCTAGGTCATACTCATAACGAATGACTTCACGATGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGACTAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa < 2:21530/141‑1 (MQ=255) tACCATATTCTAGGTCATAAATCGAACGGAGGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa > 1:782324/1‑141 (MQ=255) ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata < 2:507034/141‑1 (MQ=255) gTCATACTCATAACGAATGACTTCACGCGGTACTTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCCTTGAATAAGCTCCAGGACAGCCAATGATAAAGCCACGCCCTTCAGGTCCCACAACGATATCAACAtcccc > 2:168140/1‑138 (MQ=255) cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt > 1:322284/1‑141 (MQ=255) aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc < 1:355883/141‑1 (MQ=255) aCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtt < 1:610597/141‑1 (MQ=255) cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt > 1:603538/1‑141 (MQ=255) cATCACGAATGACTTCACGAGGTAATTTCCGTTCTTTTCTAAATGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg < 2:184048/141‑1 (MQ=255) tGACTTCACGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGCCAATCCCCATTGACTAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa < 2:518495/141‑1 (MQ=255) tCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGTGTATTCTACAAtt < 1:455395/141‑1 (MQ=255) aGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATc > 1:64255/1‑141 (MQ=255) gaTTTCCCTTCTGTGCTACCAGCTGCAAAGCCAATCCNCATTGTATAAGCCACAGGACAGCCAATGATAGAGCCACGCGCTTCAGGTCCTACAACGAGATAAACCTCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt < 2:562164/140‑1 (MQ=255) tttCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTTTTGc > 2:227916/1‑141 (MQ=255) tcttTTCTAATAGGTGCAAAGCCGATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAGGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCa < 2:290471/141‑1 (MQ=255) ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata < 2:676215/141‑1 (MQ=255) aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc > 1:718871/1‑141 (MQ=255) | AGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTC > USA300TCH1516_ALE/1744303‑1744581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |