| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,372,559 | C→T | 5.5% | C406Y (TGT→TAT) | pbuG ← | Guanine/hypoxanthine permease PbuG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,372,559 | 0 | C | T | 5.5% | 210.8 / 2.7 | 73 | C406Y (TGT→TAT) | pbuG | Guanine/hypoxanthine permease PbuG |
| Reads supporting (aligned to +/- strand): ref base C (32/37); new base T (2/2); total (34/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01 | |||||||||||
AGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGG > USA300TCH1516_ALE/2372420‑2372698 | aGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAGTATGAAGTACAGAAGGAATAGTACCATTAATACATACATTATCGGTTGTACTGCTTTATGTTGTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCcaca > 2:574375/1‑141 (MQ=255) gTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAACCATTGTAATTGGATAGAAAATAAATCCccac > 2:42178/1‑141 (MQ=255) ttttttGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCaa > 2:398149/1‑141 (MQ=255) ttttttGTGTCTGGATTTAACCAAGAACAAATATGAAGTACAAAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCaa < 1:738116/141‑1 (MQ=255) tttttGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAAt > 1:499640/1‑141 (MQ=255) tttttGTGTCTGGATTTAACCATGAACAAATACGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTGTGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAAt < 1:527089/141‑1 (MQ=255) gtCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGtt < 2:676857/141‑1 (MQ=255) gtCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGGACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGtt < 1:2293/141‑1 (MQ=255) gtCTGGATTTAACCACGAACAATTATTAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATCTTTTTCAGAGATACGCATTGTAATTGGATAGAAAATAGATCCACACGCAATACCAGtt < 1:479138/141‑1 (MQ=255) tCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTg < 1:175068/141‑1 (MQ=255) tCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTg > 1:159507/1‑141 (MQ=255) cTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGc < 1:268712/141‑1 (MQ=255) caatcaaCCATGAACCACTATGACGGAAACAATGAGAATCACCATGAACACACACAGTATCGGATGTACTTTTTTATGTTTTTGAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAAt < 2:743661/136‑1 (MQ=255) aTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAAtt < 2:564663/141‑1 (MQ=255) aTGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGa < 2:773212/141‑1 (MQ=255) gAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATa > 1:261282/1‑141 (MQ=255) aaCAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATaa < 2:203159/141‑1 (MQ=255) cAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAg < 2:108135/141‑1 (MQ=255) atGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGcatc < 1:464629/141‑1 (MQ=255) atGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGcatc < 1:448463/141‑1 (MQ=255) tGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCATACGCAATACCAGTTGCAATTGAATATGATAAAGGcatca < 2:462800/141‑1 (MQ=255) gAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGcatcat < 2:105817/141‑1 (MQ=255) gAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGAAATACCAGTTGCAATTGAATATGATAAATGcagcat > 2:662945/1‑141 (MQ=255) aGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATaa < 1:706090/141‑1 (MQ=255) gaatgaatAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCATACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTg < 1:281411/141‑1 (MQ=255) gTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAgg < 2:680967/141‑1 (MQ=255) tACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGc > 1:675828/1‑141 (MQ=255) ccATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTAGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTg > 1:698186/1‑141 (MQ=255) aaTACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGAAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGTTAAAGGCATCATAATGATTGTAATGAAAGCTGGCACt > 1:700066/1‑141 (MQ=255) catacatTATCGGATGTACTTCTTTATGTTTTTTAGAGCTTATCATGGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCaa > 2:720095/1‑141 (MQ=255) ttATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCaa > 1:72041/1‑141 (MQ=255) tCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCAACACGCAATACCAGTTGCAATTGAATATGATATTGGCGTCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAAc > 1:298967/1‑141 (MQ=255) ggATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGTCACTGCAACTTCACAACtt > 1:17763/1‑141 (MQ=255) ggATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATGGTAATGAAGGCTGGCACTGCAACTTCAAAACtt > 1:17577/1‑141 (MQ=255) gTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTcc > 2:69966/1‑141 (MQ=255) gTACTACTTAATGTTTTTTTGATATAGGCATTGCAAGAGGGAAGAGACTAAATTCACAGGCAATACCAGTTGCAATTGAATATGATCAAGGCATAATAATAATTGTAAAGAAGGCTAGAATAGCAACTTCAAAACTCTTcc > 2:385952/1‑141 (MQ=255) tACTTCTTTATGTTTTTTAGATATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCTGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGAAACTTCAAAACTTTTCCa < 1:315672/141‑1 (MQ=255) tACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGAAACTTCAAAACTTTTCCa < 1:734356/141‑1 (MQ=255) tACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAAAAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGCAGGCTGGCACTACGACTTCCAAAATTTTCCa > 2:512086/1‑141 (MQ=255) ttcttTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGtt < 1:398149/141‑1 (MQ=255) ttttttAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGc > 2:335533/1‑141 (MQ=255) ttAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAg > 2:30560/1‑141 (MQ=255) ttAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAg < 2:216516/141‑1 (MQ=255) agagATAAGCATTGTAATTGGATAGAAAATAAATCCATACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGtt > 1:444872/1‑141 (MQ=255) agaTAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTg > 1:430764/1‑141 (MQ=255) gaTAAGCATTGTAATTTGATAGAAAATAAATCCACACGCAATACCAGCTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAGGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGc < 2:325636/141‑1 (MQ=255) gCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCa < 2:157109/141‑1 (MQ=255) cATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAACATTTTCCGGTTTATTCCAGCACAGTTTGCAGCAAt > 1:322367/1‑141 (MQ=255) cATTATAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCAt > 2:546401/1‑141 (MQ=255) aTTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCAtt > 2:748342/1‑141 (MQ=255) ttGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTa > 1:321212/1‑141 (MQ=255) ttGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTa > 1:719782/1‑141 (MQ=255) ttGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTAGCTCAGCAAAGTTTGCAGCCATTa > 2:321686/1‑141 (MQ=255) gTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCGGCCATTAAt > 1:313136/1‑141 (MQ=255) gTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAAt < 2:575583/141‑1 (MQ=255) aaTTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATac < 2:646947/141‑1 (MQ=255) aTTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATaca < 1:651278/141‑1 (MQ=255) tttgATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATacac < 2:232650/138‑1 (MQ=255) ttGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATacac > 1:707379/1‑141 (MQ=255) ttGGATAGAAAATAAAACCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATacac < 2:643009/141‑1 (MQ=255) ttGGATACAAAATAAATCCACACGCAATACCAGTTGTAATTCAATATGCTAAAGGCATCATAATGATTGTAACGAAGGCTGGCCCTGCAACTTCAAAACTTCTCCAATTTATTTCTGCAAAGTTTGCAGCCATTAATacaa < 2:5563/141‑2 (MQ=255) ttGGAGAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAATGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATacac < 2:545710/141‑1 (MQ=255) tGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACAcc > 1:560927/1‑141 (MQ=255) tGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAAAACAcc > 1:245730/1‑141 (MQ=255) tGGATAGAAAACAAATCCACACGGAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACAcc < 1:230256/141‑1 (MQ=255) ggATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAGCGGCATCAGAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACGTTTCCATTTTGTCTCAGCAAAGTTTGCAGCCATTCATACACCt > 1:263931/1‑141 (MQ=255) ggATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCt < 1:693566/141‑1 (MQ=255) gtaaaaTAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTacca < 2:740191/139‑1 (MQ=255) aGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTacca < 1:42178/141‑1 (MQ=255) aaaaTAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTaccacc < 2:624652/141‑1 (MQ=255) aaataaatCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTAccacca > 1:201193/1‑141 (MQ=255) atCCATACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTCTTCCAGTTTATCTCAGCAACGTTTGCAGCCATTAATACAACTACCACCACTAAgg > 2:713386/1‑141 (MQ=255) atCCACACGCAAGTCCGGTTGCAATCGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAgg < 2:196523/141‑1 (MQ=255) tCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGc < 2:735243/141‑1 (MQ=255) ccACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCt > 2:767657/1‑141 (MQ=255) acacGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGAATGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTGCCACCACTACGGCTgg > 2:510110/1‑141 (MQ=255) | AGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGG > USA300TCH1516_ALE/2372420‑2372698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |