Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,373,506 A→T 30.0% N90K (AAT→AAA)  pbuG ← Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,373,5060AT30.0% 82.0 / 48.7 63N90K (AAT→AAA) pbuGGuanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base T (11/8);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

GCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTA  >  USA300TCH1516_ALE/2373375‑2373644
                                                                                                                                   |                                                                                                                                           
gCATTAATAATAACTTCCCTAAAAAGTCTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATccc                                                                                                                                    >  2:760554/1‑141 (MQ=255)
       aaataaCTGCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTg                                                                                                                             <  1:387546/140‑1 (MQ=255)
         ataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATATATCCACTTGTTAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGt                                                                                                                           <  1:573626/141‑1 (MQ=255)
         ataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGt                                                                                                                           <  2:604687/141‑1 (MQ=255)
         ataaCCTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGt                                                                                                                           <  1:313598/141‑1 (MQ=255)
          taaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTg                                                                                                                          >  2:216651/1‑141 (MQ=255)
           aaCTTCCCTAAACCATGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGc                                                                                                                         >  2:536484/1‑141 (MQ=255)
                  cTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGACTGCTTTTAATCCCATACCTGGTGCTAA‑CGCa                                                                                                                 >  1:264708/1‑141 (MQ=255)
                    aaaCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAAt                                                                                                               >  2:749874/1‑141 (MQ=255)
                     aaCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCCACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAAtt                                                                                                              >  1:139207/1‑141 (MQ=255)
                      aCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTg                                                                                                             >  2:15338/1‑141 (MQ=255)
                      aCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTg                                                                                                             <  1:312737/141‑1 (MQ=255)
                       cccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTCCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTgc                                                                                                            <  2:694480/141‑2 (MQ=255)
                        ccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGa                                                                                                           <  1:9846/141‑1 (MQ=255)
                        ccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATACTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGa                                                                                                           <  2:217618/141‑1 (MQ=255)
                             gACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGagcttt                                                                                                      >  1:487912/1‑136 (MQ=255)
                              aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTa                                                                                                     >  2:670072/1‑141 (MQ=255)
                              aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAAATTGCCAAGGAATACCCATGGTTAACAAAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTggcgacgga                                                                                                     >  2:118795/1‑134 (MQ=255)
                               cGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAg                                                                                                    >  2:349437/1‑141 (MQ=255)
                                 gTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCt                                                                                                  <  1:544438/141‑1 (MQ=255)
                                    aaTATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCGAA‑CGAAATTGGATATTTAGCTAtt                                                                                               >  2:750108/1‑141 (MQ=255)
                                      tatCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAg                                                                                             <  1:36666/141‑1 (MQ=255)
                                      tatCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAg                                                                                             <  2:785624/141‑1 (MQ=255)
                                             aaGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATg                                                                                      <  1:642526/141‑1 (MQ=255)
                                               gAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGaa                                                                                    >  1:277289/1‑141 (MQ=255)
                                                    taCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTGGCCCCATGAATAGCg                                                                               >  1:123969/1‑141 (MQ=255)
                                                    taCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCg                                                                               >  1:123915/1‑141 (MQ=255)
                                                        tGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGcc                                                                           <  1:503784/141‑1 (MQ=255)
                                                        tGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGcc                                                                           <  1:443516/141‑1 (MQ=255)
                                                        tGACAATACAACGGCTTGTAAACCAACTTGCCAAGGAATACCTCTTGTTACCACAACAGTAAATGCAAAGAATGCTTTTAATCCCATTCCTGTTGCTAA‑CTCAATTGGATATTTAGCTATTATCCCCATGAATAGCGAGcc                                                                           <  2:141350/141‑1 (MQ=255)
                                                         gagaATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCt                                                                          <  1:270242/141‑1 (MQ=255)
                                                          agaATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTa                                                                         <  2:282114/141‑1 (MQ=255)
                                                           gaATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTAc                                                                        >  2:188293/1‑141 (MQ=255)
                                                             aTAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACaa                                                                      <  2:85842/141‑1 (MQ=255)
                                                                      cTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTa                                                             <  1:135593/141‑1 (MQ=255)
                                                                       tGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTaa                                                            <  1:475659/141‑1 (MQ=255)
                                                                        gTTATACCAACTTGCAAAGGAATACCCATGGTTAACACCACAGTAACTGCAAAGAATGCATTCAATCCCATACCTGATGCCAA‑CGCAATTGGATGTTTAGCTATTAGCCCCATGAATAGCGAGCCTCCAAATGCTGCCAAt                                                           >  2:220159/1‑141 (MQ=255)
                                                                                  cTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGACTACAAGTTCTGCTAATGCAGTCGCTa                                                 >  1:212707/1‑141 (MQ=255)
                                                                                    tGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCAATGAATAGCGAGCCTACAAATGGTGCTAATGCAGTCGCGACa                                               >  2:551581/1‑141 (MQ=255)
                                                                                     gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaa                                              >  2:711031/1‑141 (MQ=255)
                                                                                     gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaa                                              >  1:285384/1‑141 (MQ=255)
                                                                                             attcccATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCa                                      <  1:743743/138‑1 (MQ=255)
                                                                                             aTACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCa                                      <  1:412127/141‑1 (MQ=255)
                                                                                                   aTGGTTAACACAACAGTAAATGTAAAGAAGGCATTTAATCCC‑TACTTTGTACCAAGCTCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTgg                                <  1:771188/141‑1 (MQ=255)
                                                                                                   aTGGTTAACACAACAGTAAACGCAAACAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTgg                                <  1:457185/141‑1 (MQ=255)
                                                                                                    tGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGt                               <  1:760554/141‑1 (MQ=255)
                                                                                                     ggTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATACCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTc                              <  1:223000/141‑1 (MQ=255)
                                                                                                        tAACACAACAGTAAATGCAATGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAt                           <  1:15338/141‑1 (MQ=255)
                                                                                                        tAACACAACAGTAAATGCAAAGAATGCTTTTTATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAt                           >  1:219931/1‑141 (MQ=255)
                                                                                                        tAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAt                           >  2:129882/1‑141 (MQ=255)
                                                                                                          acacAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                         >  2:11382/1‑141 (MQ=255)
                                                                                                          acacAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCCACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                         >  2:671824/1‑141 (MQ=255)
                                                                                                              aacaGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCAGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCat                     >  2:404544/1‑141 (MQ=255)
                                                                                                                   tAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtctt                <  1:418058/141‑1 (MQ=255)
                                                                                                                   aaaaTGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtctt                <  2:568526/140‑1 (MQ=255)
                                                                                                                    aaaTGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCACCATGAATAGCGAGCCTACAAACGCTGCGAATGCAGTCGCTACAAAAATGGCACCTTGGTACATTCTCATAtcttc               >  2:278243/1‑141 (MQ=255)
                                                                                                                    aaaTGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttc               >  1:677173/1‑141 (MQ=255)
                                                                                                                     caTGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATGTTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTACTATTTCAGTCGCTACAAACATGGCACCTTGGTCCATTTTCATAtgttct              <  2:479737/140‑1 (MQ=255)
                                                                                                                     aaTGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttct              >  1:629449/1‑141 (MQ=255)
                                                                                                                       tGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTCGCTATTAGCCACATGAATAGCGAGCCTACCAATGCTGCTAATGCAGTCGCTGCAGAAATGGCACCTTGGTCCATTTTCATATATTCTGa            >  1:194294/1‑141 (MQ=255)
                                                                                                                            aGAATGCTTTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc       >  1:517771/1‑141 (MQ=255)
                                                                                                                            aGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc       <  1:87968/141‑1 (MQ=255)
                                                                                                                                 gCATCTAATCCAAGACCTCGTGCAAA‑CGCAATTAGATATTTAGCTATTAGCCCCAGGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCATCTTGGTCCATTTTCATATCTTCTGATACGCCTTTa  <  1:430065/141‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                           
GCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAA‑CGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTA  >  USA300TCH1516_ALE/2373375‑2373644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑