Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,701,061 C→T 8.7% M266I (ATG→ATA)  bacF ← Transaminase BacF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,701,0610CT 8.7% 184.7 / 5.6 68M266I (ATG→ATA) bacFTransaminase BacF
Reads supporting (aligned to +/- strand):  ref base C (30/30);  new base T (3/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAT  >  USA300TCH1516_ALE/2700923‑2701200
                                                                                                                                          |                                                                                                                                           
aTGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAACGCATATATTGCGGCCTCTTGTAATGCACCAAACAt                                                                                                                                           >  1:132919/1‑141 (MQ=255)
             aaaTCCGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtg                                                                                                                              <  1:238934/141‑1 (MQ=255)
             aaaTCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtg                                                                                                                              <  1:705514/141‑1 (MQ=255)
              aaTCAGCTTTTGCAAGCATGGCTTCGAATCCGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtgt                                                                                                                             <  1:389228/141‑1 (MQ=255)
                    cTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGttt                                                                                                                       >  1:631050/1‑141 (MQ=255)
                        tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  2:621753/141‑1 (MQ=255)
                        tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  2:487678/141‑1 (MQ=255)
                        tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  1:675784/141‑1 (MQ=255)
                          cAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACt                                                                                                                 >  2:302294/1‑141 (MQ=255)
                             gCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTCCtttt                                                                                                              >  2:753130/1‑141 (MQ=255)
                             gCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACtttt                                                                                                              >  2:6438/1‑141 (MQ=255)
                                tGGCTTCGTATCGGTCACGAAGTGTGGTCATTTCATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCa                                                                                                           <  1:178454/141‑1 (MQ=255)
                                   cTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAg                                                                                                        >  2:711557/1‑141 (MQ=255)
                                         aTCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAGGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCGAAGCTTGaa                                                                                                  >  1:374128/1‑141 (MQ=255)
                                             gTCACGACGTGTTTTAGATACGTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTCCTTTTTCAAAGCTTGAATCat                                                                                              >  1:406987/1‑141 (MQ=255)
                                                  gacgTGTTTTAAATACCTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCtt                                                                                         <  1:147570/141‑1 (MQ=255)
                                                           tAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTCTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCaa                                                                                >  1:558986/1‑141 (MQ=255)
                                                               tACCTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGc                                                                            <  1:301113/141‑1 (MQ=255)
                                                                      cTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATACTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCg                                                                     <  2:678047/141‑1 (MQ=255)
                                                                          gTTCTTCTAAAAAATCATCATAATGATTCAACGCAATTATTGCGGCAGCAAGGAATGCACTTAACATCCCATCATGTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCCTATCTTTATTACCAACTGCAAAACCGActc                                                                 >  1:465089/1‑141 (MQ=255)
                                                                          gTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGActc                                                                 >  1:614176/1‑141 (MQ=255)
                                                                            tcttctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACAACAAATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTa                                                               >  1:496989/1‑141 (MQ=255)
                                                                             cttctAAAAAATCATCATACGGATGCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGAATCTaa                                                              >  2:364570/1‑141 (MQ=255)
                                                                             cttctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTaa                                                              >  2:773827/1‑141 (MQ=255)
                                                                               tgtAAAAAGACAGCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTaaaa                                                            <  2:306952/139‑1 (MQ=255)
                                                                               tctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTCTGCGTTCGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAGCTGCAAAACCGACTCGaaaa                                                            >  1:265079/1‑141 (MQ=255)
                                                                                 tAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAAcc                                                          <  2:304563/141‑1 (MQ=255)
                                                                                  aaaataTCATCATGATGACTCAAATCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGTATTTGTGTGCGTTTGGTACTTTTTCAAAGCTCGAATCATATCTTTATTACCAACTGCAAAATCGACTCTAAATCCt                                                         <  2:710531/141‑1 (MQ=255)
                                                                                  aaaaaaTCATCATAATGATTCAAATCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCTTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  1:511564/141‑1 (MQ=255)
                                                                                  aaaaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  2:631050/141‑1 (MQ=255)
                                                                                  aaaaaaTCAGCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATGTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  2:7242/141‑1 (MQ=255)
                                                                                   aaaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTg                                                        <  2:271777/141‑1 (MQ=255)
                                                                                    aaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGa                                                       <  1:374278/141‑1 (MQ=255)
                                                                                      aaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACa                                                     <  1:692231/141‑1 (MQ=255)
                                                                                       atcatcATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACAt                                                    >  2:482308/1‑141 (MQ=255)
                                                                                       atagtcacAATGATTCAAGCCATATATTGCGGCATCTTGTAATGCACCAAAAGTCCCAGCATTTGTGTGCGATTGGTAATTGTTCAAAGCTTGAATCATATCTTTATTACCAACTTTAAAACCGCCTCTAAAACCTTACAt                                                    <  2:401952/137‑1 (MQ=255)
                                                                                        tcatcaTAATGATTCAAAGCATATATTGCGGCGTCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATg                                                   <  1:103578/141‑1 (MQ=255)
                                                                                         catcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  2:20618/141‑1 (MQ=255)
                                                                                         catcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  1:138962/141‑1 (MQ=255)
                                                                                         cagcatAATTATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACAGCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  2:103348/138‑1 (MQ=255)
                                                                                          atcatAATTCGTCAAAGCATATATTGCGGCATCTTGTAATCCACCACACATCCCAGCATGTGTGTTCGTTTAGTACTTTTTCAAAGCTTTAATCATATCTTTATTACCACCTGTAAAACCCACGCTAAAACCTGACATGtt                                                 <  2:374128/141‑1 (MQ=255)
                                                                                           tcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTCTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAAGCGACTCTAAAACCTGACATGTta                                                <  2:564160/141‑1 (MQ=255)
                                                                                           acatGATGAGTGAAAGCATATATTGCGGCATCTTGTAATTCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATACCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTta                                                <  2:118969/140‑1 (MQ=255)
                                                                                            catAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTtat                                               >  1:276645/1‑141 (MQ=255)
                                                                                             atAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTtata                                              <  1:624571/141‑1 (MQ=255)
                                                                                              tagtGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAACCATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATaa                                             <  2:646547/138‑1 (MQ=255)
                                                                                                aTGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAAcc                                           <  2:49424/141‑1 (MQ=255)
                                                                                                    ttCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTa                                       <  2:42429/141‑1 (MQ=255)
                                                                                                         aGCATATATTGCGGCATTTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACaa                                  <  2:446103/141‑1 (MQ=255)
                                                                                                         aGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTCCGGTCTTACATGTTATAACCTTTAGACaa                                  <  2:760690/141‑1 (MQ=255)
                                                                                                         aGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGCCaa                                  >  2:526807/1‑141 (MQ=255)
                                                                                                          gCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCTACTCTAAAACCTGACATGTTATAACCTTTAGACAAg                                 >  1:437715/1‑141 (MQ=255)
                                                                                                           cATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACaaag                                >  1:624903/1‑139 (MQ=255)
                                                                                                           cATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAAATGCAGAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAgg                                >  1:146229/1‑141 (MQ=255)
                                                                                                             tatatTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGaa                              >  2:527586/1‑141 (MQ=255)
                                                                                                                       cATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACCAGGAATAAATTTCaa                    >  1:86592/1‑141 (MQ=255)
                                                                                                                       cATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCaa                    >  2:324456/1‑141 (MQ=255)
                                                                                                                        aTCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAAt                   >  1:699367/1‑141 (MQ=255)
                                                                                                                         tCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATc                  >  2:41262/1‑141 (MQ=255)
                                                                                                                          cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  1:266964/1‑141 (MQ=255)
                                                                                                                          cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  1:737696/1‑141 (MQ=255)
                                                                                                                          cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  2:474169/1‑141 (MQ=255)
                                                                                                                            tGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCCTTAGACAAGGAATAAATTTCAATCGCa               >  2:595822/1‑141 (MQ=255)
                                                                                                                                aTGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACAt           <  1:136997/141‑1 (MQ=255)
                                                                                                                                   cACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTGGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCtt        >  1:347703/1‑141 (MQ=255)
                                                                                                                                      cAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATTTTATAACCTTTATACAAGGAATAAATTTCAATCGCAACATCTTTcc     >  1:352968/1‑141 (MQ=255)
                                                                                                                                      cAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACAGCTTTcc     >  2:191415/1‑141 (MQ=255)
                                                                                                                                         aCATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAt  >  2:725960/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAT  >  USA300TCH1516_ALE/2700923‑2701200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑