BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,873	A→G	7.5%	D771D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,873	0	A	G	7.5%	251.8 / 2.4	104	D771D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (57/37); new base G (6/3); total (63/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTC  >  USA300TCH1516_ALE/2774733‑2775013

gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAATCGCTGTCTGAATCTGAATCACTTTCTGAATCCGAATCGCTatctgaa                                                                                                                                              >  1:340957/1‑141 (MQ=255)

 ggtcGCGAACTGAAGGCGTGTCGCTTTCCGGGTCTGAGTCGCTTTCTGAGTCTGAATCGCTGTCGGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaat                                                                                                                                             <  2:122354/140‑1 (MQ=17)

   tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTgtctgaatct                                                                                                                                           >  1:187327/1‑141 (MQ=255)

   tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatct                                                                                                                                           >  1:618123/1‑141 (MQ=255)

    cGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctg                                                                                                                                          >  1:507911/1‑141 (MQ=255)

      cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTAtctgaatctgaa                                                                                                                                        >  1:412595/1‑141 (MQ=255)

        aTCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                                                                                      <  1:544580/141‑1 (MQ=255)

           tGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCGatctgagtctgactgggt                                                                                                                                   >  2:315349/1‑135 (MQ=11)

           tGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                                                                                                   <  1:713116/141‑1 (MQ=255)

                  gAGTCGCTATCCGGGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAg                                                                                                                            <  1:17575/141‑1 (MQ=255)

                  gAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAATCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTGTCCGAg                                                                                                                            >  2:476514/1‑141 (MQ=255)

                   aGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTGTGAGTTTGAGTCGCTGTCCGGGTCTGAGTCGCTGTCTGGATCTGAATCGGTGTCTGAGTCCGAGTCGCTATCGGAGTCTGAATCGCTGTCCGAGt                                                                                                                           >  2:678787/1‑141 (MQ=14)

                   aGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGGCTGAATCGCTGTCTGAGTCCGAATCGCCATCTGAGTCTGAATCACTATCCGAgg                                                                                                                           >  2:42846/1‑140 (MQ=14)

                   aGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAGTCGCTATCTGAGTCTGAGTCGCTATCCGAGt                                                                                                                           >  2:8427/1‑141 (MQ=14)

                       gCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                                                                                       <  1:568007/141‑1 (MQ=255)

                       gCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                                                                                       >  1:132705/1‑141 (MQ=255)

                        cTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAATCTGAATCGCGTTCCGGGCCTgga                                                                                                                      >  2:614654/1‑139 (MQ=17)

                         tATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGt                                                                                                                     >  1:747099/1‑141 (MQ=255)

                          aTCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGATGCTGAGTCGCTGTCTGAAGCTGAATAGCTGTCGGAATCAGAGGAGATATCCAAAGCTGAATGGCTATCCGAGATTGAGTc                                                                                                                    >  2:163366/1‑141 (MQ=14)

                          aTCCGAGGCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTc                                                                                                                    <  2:112529/141‑1 (MQ=255)

                           tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCg                                                                                                                   >  1:492627/1‑141 (MQ=255)

                            tttgttCTGTGTCGCTATTTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                                  <  2:78240/136‑1 (MQ=255)

                             cGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCt                                                                                                                 <  1:737576/141‑1 (MQ=255)

                                 tCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCAGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAGCCGCTCTCTGAATCTGAATCCCTATCTGAGTCTGAGTAGCTGgct                                                                                                             >  2:148323/1‑141 (MQ=14)

                                  cTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGGCTGAATCCGAATCGCGATCTGAATCTGAATCGCTATCCGAGTCTGAGTACCTGtcta                                                                                                            >  2:68101/1‑140 (MQ=255)

                                   ggAGTCGCTATCTGGGTCTGAATCGCGGTCTGGGTCTGTGCGGGTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctga                                                                                                           <  1:608493/140‑1 (MQ=18)

                                    gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTGTCTGAATCTGAATCGCTGTCCGAGTCTGAGTCGCTGtctgag                                                                                                          >  2:237346/1‑140 (MQ=255)

                                    gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCGGTCTGAATCTGAATCGCTGTCCGCGTCTGAGTCGCTGtctgaa                                                                                                          >  2:505228/1‑141 (MQ=17)

                                    gAGTCGCTATCTGAGTATGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaa                                                                                                          >  1:391584/1‑141 (MQ=255)

                                     aGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCAGAATCGCTATCTGAATCTGAATCGCTATCGGAGCCTGAGACGCTGtctgact                                                                                                         >  1:355570/1‑139 (MQ=255)

                                           tATCTGAGTCTGATTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTctgaatctgaat                                                                                                   <  1:200233/141‑1 (MQ=255)

                                             tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTGGCTATCCGAGTCTGAGTCGCTGTCGTAATCTTAATCACTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCCGCGTCTGAGGCGCTGtctgactctgagttg                                                                                                 >  2:166807/1‑137 (MQ=14)

                                             tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCGGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCg                                                                                                 >  1:701689/1‑141 (MQ=255)

                                              cTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGc                                                                                                <  1:124267/141‑1 (MQ=255)

                                               tGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                                               >  1:675002/1‑141 (MQ=255)

                                                      gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTCTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                                                        <  2:593797/141‑1 (MQ=255)

                                                      gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                                                        >  1:417860/1‑141 (MQ=255)

                                                      gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGGCTGAGTCGGTGTCTGACACTAAATCATTGTCTGGAACCGAATCGCTATCTGAATCTGAATCGCTATCCTATTCTGCGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                                                        <  1:148917/141‑1 (MQ=18)

                                                       aaTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAAACCGAATCGCTGTCTGAga                                                                                       >  2:203179/1‑140 (MQ=255)

                                                        aTCGCTGTCTGAGTCTGAGTCGCTATCCGAGCCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTACATGACTCTCTGAGTc                                                                                      >  2:122650/1‑141 (MQ=17)

                                                         tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCAGTGTCTGAGTCCGAGTCGCTATCTGAAGCTGAATCGCTATCGGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGtgc                                                                                     >  2:284320/1‑139 (MQ=17)

                                                         tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTcc                                                                                     >  1:282043/1‑141 (MQ=255)

                                                          cGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCAGTATCGCTGTCTGAGACCg                                                                                    >  2:239025/1‑141 (MQ=255)

                                                          cGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                                    <  1:785750/141‑1 (MQ=255)

                                                          cGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                                    <  1:722208/141‑1 (MQ=255)

                                                            cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                                                  <  2:632540/141‑1 (MQ=255)

                                                            cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                                                  <  1:644034/141‑1 (MQ=255)

                                                            cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                                                  <  1:685941/141‑1 (MQ=255)

                                                              gtctgagtctgaGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTGTCCGAGTCTGAGTCGCTTTGTGAAACCGAATCGCTGGCTGAGTctgcagg                                                                                >  2:19929/1‑135 (MQ=17)

                                                               tctgattctgagTCGCTATCGGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGACTCTGAATCGCTATCCGAGTCTTAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCg                                                                               <  2:325709/141‑1 (MQ=255)

                                                                 tgagtatgagtcGCTATCAGAGTCTGAGGCGGTGTCTGAATCTGAATCACTGTCTGAATCCGAATCACTATCTGAATCTGAATCTCTGTCCGAGTCTGAGTCGCTGTCTGCATCTGAATCGCCGCCTGAGTCTGAATCggt                                                                             >  1:581289/1‑139 (MQ=17)

                                                                  gagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa                                                                            >  1:2913/1‑141 (MQ=255)

                                                                  gagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa                                                                            >  1:622616/1‑141 (MQ=255)

                                                                  gagtctgagtcGCTATCCGAGTCTGAGCCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCGGGATCTGAATGGCTGTCTGAGTCCGACTGGCtc                                                                            >  1:130062/1‑140 (MQ=18)

                                                                  gagtccgagtcGCTATCCGAGTCTGAGTCGCTGTTTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa                                                                            <  1:339102/141‑1 (MQ=255)

                                                                     tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTatct                                                                         >  1:721687/1‑141 (MQ=255)

                                                                     tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatct                                                                         <  1:319627/141‑1 (MQ=255)

                                                                       tgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga                                                                       >  1:324813/1‑141 (MQ=255)

                                                                         agtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAGTGTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCGGAGTCGCTatctgagt                                                                     >  2:249088/1‑139 (MQ=14)

                                                                           tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatct                                                                   >  1:216592/1‑141 (MQ=255)

                                                                             gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATATGAATCGCTATCTGAATCTGAATCGCTATTAGTGTGTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTgtctgaatctta                                                                 >  2:657201/1‑139 (MQ=17)

                                                                               tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGt                                                               >  1:565913/1‑141 (MQ=255)

                                                                                 tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCg                                                             >  1:761555/1‑141 (MQ=255)

                                                                                   cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCt                                                           >  1:365952/1‑141 (MQ=255)

                                                                                    gattccgAGTCGCTATCCGAATCTGATTCGCTGGCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                                          <  2:658694/135‑1 (MQ=11)

                                                                                        cTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCGGAATCTGAGTCTCTATCCGAGTCTGAGGCGCTGTCTGAATCTGGATCGCTGCCTGAGTCCGAATCGCTATCTGAATCTGATTCGCTgtcta                                                      >  1:25906/1‑140 (MQ=17)

                                                                                         tGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCGGAGTCGCTgtctgg                                                     >  1:218477/1‑140 (MQ=255)

                                                                                           aGTCTCTTTCTGAATCTGAATCACTGTCTGAGTCTGAGTCGCTATCTGAATCCGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                   <  2:164376/141‑1 (MQ=14)

                                                                                           aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                   >  1:681922/1‑141 (MQ=255)

                                                                                             tCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGGGTCCGAATCGCTATCTGAATCTGAGTCGATgtctgagtcg                                                 >  2:328922/1‑140 (MQ=255)

                                                                                             tCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCGACCTGAATCTGAGTCGCTgtctgagtcg                                                 >  2:469845/1‑140 (MQ=255)

                                                                                             tCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTAGCTGAGTCTGAATCGCTGGCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGAGTCTGAGGCGCTggctgagtct                                                 >  2:328882/1‑141 (MQ=11)

                                                                                             gcGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTTTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                                 <  2:245917/140‑1 (MQ=255)

                                                                                               tcgtgctgcatctgaatCGCGTGCTGATGCTGAGTCTCTATCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTgtctgagtctga                                               <  2:388410/136‑1 (MQ=14)

                                                                                                   tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCg                                           <  1:685002/141‑1 (MQ=255)

                                                                                                    ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAATCGc                                          >  1:568678/1‑141 (MQ=255)

                                                                                                    ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc                                          <  1:312462/141‑1 (MQ=255)

                                                                                                     tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAAgcgcg                                         >  2:446274/1‑140 (MQ=255)

                                                                                                      gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGATTCTGAATGGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                        >  1:775706/1‑141 (MQ=255)

                                                                                                        atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                      <  1:557663/141‑1 (MQ=255)

                                                                                                         gctgaatcAGTGTCTGAATCCGAATCGCTATCTGAATCCGAGTCGGTTTCCAAGGCATAGTCGATGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                                     <  1:372747/140‑1 (MQ=14)

                                                                                                             atggaCTCTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                 <  1:174501/137‑1 (MQ=255)

                                                                                                             aatcACTGTCTGAATCCGAATCGCTCTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGTGCCGCCGTCGGAGTCTGAATCGCTatctgaat                                 <  1:189393/141‑1 (MQ=17)

                                                                                                             aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTAAGTCCTAGTCGCACTGAGATACGTTGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                 <  1:778634/141‑1 (MQ=17)

                                                                                                               tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                               >  1:764405/1‑141 (MQ=255)

                                                                                                                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGCCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATAGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgattctg                              >  2:514587/1‑141 (MQ=255)

                                                                                                                aaCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                              >  2:683318/2‑141 (MQ=255)

                                                                                                                  cTGTCTGACTCCGAATCGCTATCTGAATCTGAATCGTTTTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                            <  2:545300/141‑1 (MQ=255)

                                                                                                                  cTGTCTGAATCCGAATCGCTATCTGAACCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGATTCTGAATCGCTATCTGAATCTGAg                            <  1:505725/141‑1 (MQ=255)

                                                                                                                        caaTCCGAATCGCTATCTGAATCTGAATCGCTATCCGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTa                      <  2:690137/140‑1 (MQ=255)

                                                                                                                         aaTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATCTGATGCGCTAt                     >  1:529585/1‑141 (MQ=255)

                                                                                                                         aaTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCggtgt                     >  2:525933/1‑137 (MQ=255)

                                                                                                                              gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgag                >  1:495243/1‑141 (MQ=255)

                                                                                                                                 tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCGGAGTCGCTAtctgagtct             >  1:212703/1‑141 (MQ=255)

                                                                                                                                 tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGACTCTGAGTCGCTAtctaagtcg             >  2:565506/1‑140 (MQ=255)

                                                                                                                                   gCTATCTGAATCTGAATCGCGATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctga           <  1:584160/141‑1 (MQ=255)

                                                                                                                                   gCTATCTGAACCTGAATCGCTATCCGAGTCTGCGTCGCTGTCTGATTCTCAAGCCCCGTCTAAATCCGAATAGCGATCTGAATATGAATCACTGTCAGTATCAGAAGCGCTGTCTGAAACTGAGTCGCTGgcttaggctga           >  2:35843/1‑141 (MQ=9)

                                                                                                                                    cTATCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTTAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgag          <  1:163366/141‑1 (MQ=255)

                                                                                                                                            atctgaattGCTATCCGAGTCTGAGTTGGTGTACGAATCTAGATCGCTGTCTTAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc  <  1:365214/141‑1 (MQ=14)

                                                                                                                                            atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc  <  1:700153/141‑1 (MQ=255)

GAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTC  >  USA300TCH1516_ALE/2774733‑2775013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`