Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 111,560 T→C 10.3% R210R (CGT→CGC)  USA300HOU_RS00520 → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5600TC10.3% 213.5 / 10.4 84R210R (CGT→CGC) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (4/5);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111422‑111699
                                                                                                                                          |                                                                                                                                             
tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                             >  1:119914/1‑141 (MQ=255)
tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                             <  1:42767/141‑1 (MQ=255)
  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                                                                                                           >  1:385954/1‑141 (MQ=255)
  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAATTAACTAAGGATGATTGTAATGTAAAACAATTACGCaaaa                                                                                                                                           >  2:432554/1‑141 (MQ=18)
     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                                        <  1:790617/141‑1 (MQ=255)
     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                                        <  1:213639/141‑1 (MQ=255)
     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGat                                                                                                                                        <  1:232800/141‑1 (MQ=255)
       tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGatat                                                                                                                                      >  1:667110/1‑141 (MQ=255)
       tttGCGCAAAGTAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATGATGTAAAACAATTACGTAAAAGatct                                                                                                                                      >  1:578154/1‑139 (MQ=255)
          gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTCATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAc                                                                                                                                   <  1:540883/141‑1 (MQ=255)
           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAAGATGAAGTAACTAATGACGCTACCATGGTAAAACAATTAAGCAAAAGATATGata                                                                                                                                  >  1:694151/1‑139 (MQ=14)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGatat                                                                                                                                 <  1:691751/141‑4 (MQ=14)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACaa                                                                                                                                 >  1:635318/1‑140 (MQ=255)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                                 <  1:688500/141‑1 (MQ=255)
            gcAATAGTGCTATTTTAAAGATTTAATGAATTATAAAGATGGAGTTATATCATATAATCCAGAGGTGCCGCGTCATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAACACAATCACGCAAAAgatatgatat                                                                                                                                 <  2:5015/141‑1 (MQ=14)
             cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGatgta                                                                                                                                >  1:590731/1‑137 (MQ=255)
                 ataACAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaa                                                                                                                            <  2:134844/141‑1 (MQ=255)
                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacga                                                                                                                         <  1:100577/141‑6 (MQ=11)
                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                         <  1:373799/141‑1 (MQ=255)
                    gCAATTTTAAAGAGTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                         <  2:120513/141‑1 (MQ=255)
                       aTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGTCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAACGTAAAACAATTACGTAAAAGATATGACATACCAACAAATa                                                                                                                      <  1:581597/141‑1 (MQ=255)
                       aTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATa                                                                                                                      <  2:60480/141‑1 (MQ=255)
                                  ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                                                                           <  2:65312/141‑1 (MQ=255)
                                    aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                                                                         >  1:617196/1‑141 (MQ=255)
                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                        <  2:643269/141‑1 (MQ=255)
                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                        <  2:617470/141‑1 (MQ=255)
                                     aTGAATTATAAAGATGGAAATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTACAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                        <  1:579346/141‑1 (MQ=255)
                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                       >  1:486073/1‑141 (MQ=255)
                                      tGAATTATAAAGATGGAGAAATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGACATGACATACCAACAACTAAAGCGCCGAAACtgt                                                                                                       >  2:442922/1‑141 (MQ=255)
                                       gAACTATCAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                                                                      <  1:78560/141‑1 (MQ=255)
                                        aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                     <  1:700443/141‑1 (MQ=255)
                                        aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                     <  1:523826/141‑1 (MQ=255)
                                               aaGATGGAGATATATCATATAATCCAGAGGTGCGGAGTTATTCAGCTCAATATCAATTAACTAATGATGAGTATAATGTAAAACAATTACGTAACAGATATGACATACCAATAAATAAAGCTCCGAAGCTGCTCTTGAAAg                                                                                              >  2:115515/1‑141 (MQ=17)
                                               aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACACTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                              >  2:115253/1‑141 (MQ=255)
                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAg‑gg                                                                                     <  2:511496/141‑1 (MQ=255)
                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCCGTTGTTGAAAGGTACAg‑gg                                                                                     >  2:249271/1‑141 (MQ=255)
                                                       aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAGAGGACATACCAACTAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAg‑gg                                                                                     <  1:112114/140‑1 (MQ=255)
                                                         tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGaa                                                                                   <  2:412258/141‑1 (MQ=255)
                                                           tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAAtt                                                                                 <  2:318436/141‑1 (MQ=255)
                                                             tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATACCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTCGTTGAAAGCAACAG‑GGAATTTa                                                                               >  2:583547/1‑141 (MQ=255)
                                                             tCATATAATCCAGAGGTGCCGAGCTAGTCAGCCCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAAtaaa                                                                               >  2:354138/1‑138 (MQ=255)
                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGtt                                                                        <  2:610774/141‑1 (MQ=255)
                                                                     tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGCATTTAAAAGGTtc                                                                       >  1:125949/1‑141 (MQ=255)
                                                                     accAGAGGTGTCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTtc                                                                       <  2:194897/140‑1 (MQ=255)
                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTtca                                                                      >  2:296442/1‑141 (MQ=255)
                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGT‑AAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTtcat                                                                     <  1:298004/141‑1 (MQ=255)
                                                                       cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGATGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGGtcat                                                                     >  2:254816/1‑141 (MQ=255)
                                                                         gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTtcatca                                                                   <  1:266755/141‑1 (MQ=255)
                                                                          agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAg                                                                  >  2:763095/1‑141 (MQ=255)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGt                                                                 >  1:616771/1‑141 (MQ=255)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGt                                                                 >  1:120946/1‑141 (MQ=255)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGt                                                                 >  1:431045/1‑141 (MQ=255)
                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGtt                                                                >  1:648144/1‑141 (MQ=255)
                                                                               ccGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTGGGa                                                             >  2:498048/1‑141 (MQ=255)
                                                                                   gggaTTGAGCTCAATATGAATTAACTAAGGATGATTAGAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCAGAAGCTGTTGTTTAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGatgaa                                                         >  2:694391/4‑138 (MQ=14)
                                                                                   gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATGAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTAATCAGTTGGAtat‑a                                                        >  2:566040/1‑141 (MQ=255)
                                                                                   gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGAtat‑a                                                        >  1:776411/1‑141 (MQ=255)
                                                                                         cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑aaaaaaa                                                  <  1:45322/141‑1 (MQ=255)
                                                                                          aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAAt                                                 <  2:604056/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAAt                                            <  1:521359/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATGGAAt                                            >  1:335004/1‑141 (MQ=255)
                                                                                                  atCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTa                                         >  1:603091/1‑141 (MQ=255)
                                                                                                     aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACtt                                      >  1:11004/1‑141 (MQ=255)
                                                                                                      aTTAACTAATGATGATTAAAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACttt                                     <  2:448951/141‑1 (MQ=255)
                                                                                                           cTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTAATCAGCTGGATAT‑AAAAAAATTAAATTTACTTTTTTag                                >  2:60940/1‑141 (MQ=255)
                                                                                                               agatgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:123730/140‑1 (MQ=255)
                                                                                                               agatgatTATAAAGTAACACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:2527/140‑1 (MQ=255)
                                                                                                                 atgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATa                          >  1:764230/1‑141 (MQ=255)
                                                                                                                  tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATaa                         >  1:86015/1‑141 (MQ=255)
                                                                                                                   gatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTAACTTTTTTAGAGCATaaa                        >  1:155910/1‑141 (MQ=255)
                                                                                                                       ataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAGGAGGGGCATCAGTCGG‑TATAAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                    <  1:778576/141‑1 (MQ=18)
                                                                                                                          aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 <  1:643128/141‑1 (MQ=255)
                                                                                                                          aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:748653/1‑141 (MQ=255)
                                                                                                                            gTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCTTTTGGATAT‑AAAAAAATTGAATTTACCTTTTTAGAGAATAAAAATGAAAat               <  1:576650/141‑1 (MQ=255)
                                                                                                                             tAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata              >  1:434773/1‑141 (MQ=255)
                                                                                                                               aaaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt            >  1:756441/1‑141 (MQ=255)
                                                                                                                               aaaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTAAAAGGTACAG‑GGAATTTAAAAGGTTCAGCAGCTCAATAT‑AAAAAAATTGAATTTACNTCTNTNGCGTATAAAAATGAAAagatc            >  1:552790/1‑140 (MQ=17)
                                                                                                                                aaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAGGGTTAATAGACTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttt           >  1:537703/1‑141 (MQ=21)
                                                                                                                                  cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         <  2:518706/141‑1 (MQ=255)
                                                                                                                                  cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         <  2:635318/141‑1 (MQ=255)
                                                                                                                                    aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAAAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAAAAtttactt       >  2:201510/1‑141 (MQ=255)
                                                                                                                                        cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact   >  2:508588/1‑141 (MQ=255)
                                                                                                                                         gTAAAAGATATGACATACCAATAAATAAAGCGCCGAAGCTGTTGCGGAGAGGGACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  <  2:671391/141‑1 (MQ=21)
                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCCCAGAACTTG‑AGTTAGAACGGACGGAGGAAATTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  1:379681/1‑141 (MQ=17)
                                                                                                                                          |                                                                                                                                             
TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAG‑GGAATTTAAAAGGTTCATCAGTTGGATAT‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111422‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑