Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 462,218 A→T 11.2% K329I (AAA→ATA)  USA300HOU_RS02185 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE462,2180AT11.2% 220.3 / 8.3 81K329I (AAA→ATA) USA300HOU_RS02185hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/41);  new base T (4/6);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAT  >  USA300TCH1516_ALE/462078‑462357
                                                                                                                                             |                                                                                                                                            
gAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGaaaa                                                                                                                                              >  1:167283/1‑141 (MQ=255)
 aaaaaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTACTTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGaaaaa                                                                                                                                             >  2:378186/1‑141 (MQ=255)
 aaaaaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGaaaaa                                                                                                                                             <  1:668750/141‑1 (MQ=255)
    aaTGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAaaaagta                                                                                                                                          >  1:652837/1‑141 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAaaaagtaa                                                                                                                                         <  1:703632/141‑1 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAaaaagtaa                                                                                                                                         <  1:339125/141‑1 (MQ=255)
     atgatgAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGGGGAaaaagtaa                                                                                                                                         >  1:33275/1‑141 (MQ=255)
       gatgaGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACAAAAGTGGAaatagtaaaa                                                                                                                                       >  2:468336/1‑141 (MQ=255)
       gatgaGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGGTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAaaaagtaaaa                                                                                                                                       >  2:218424/1‑141 (MQ=255)
          gagaTCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAaaagtaaaagta                                                                                                                                    >  1:154054/1‑141 (MQ=255)
          gagaTCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAaaagtaaaagta                                                                                                                                    >  1:648286/1‑141 (MQ=255)
              tctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGa                                                                                                                                <  1:485455/141‑1 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAGAGTAAAAGTATTGaa                                                                                                                               >  1:492782/1‑141 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGaa                                                                                                                               >  1:44738/1‑141 (MQ=255)
               ctcATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGGAACGAGTTTAGAAGATATTGATAGAGCAGAAACTAAATATG‑ACCATACTAAAGTGGGAAAGGAAAAAGTGTTaaa                                                                                                                               >  1:357212/1‑141 (MQ=255)
                tcATTAACATTTGCACCTGATGACGAAGATGAGGAATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGaaa                                                                                                                              <  2:252488/141‑1 (MQ=255)
                   ttAACATTTGCACCTGATGACGAAGACGAGCCATCGAACTCACGAGTTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGAt                                                                                                                           <  1:216423/141‑1 (MQ=255)
                     aaCATTTGCACCTGATGACGAAGATGAGGCATTGAAGTGGTGAATGTGCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAGTAGTAAAAGTATTGAAAGATGt                                                                                                                         >  1:417839/1‑141 (MQ=255)
                       cATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       >  1:636997/1‑141 (MQ=255)
                       cATTTGCACCTGATGACGAAGATAAGGTATTGAATGCAGTAACTTCCCAAACTCAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCCTACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       <  1:694166/141‑1 (MQ=255)
                       aaTTTGCAACTGATGACGAAGATGAAGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ATCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTa                                                                                                                       <  1:563176/140‑1 (MQ=255)
                        aTTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTaa                                                                                                                      <  1:198590/141‑1 (MQ=255)
                              aCCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAagaaga                                                                                                                >  2:4419/1‑141 (MQ=255)
                                cTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGAtt                                                                                                              >  1:269853/1‑141 (MQ=255)
                                       cGAAGATGAGACATTGAAGTCATTAATTTTCAAAACGAAGGTCACCAGCGTATTAGAGATTTTTAAAGCAGAAACGAAATATG‑ACCATACTAAAGTGGAAAAGGTAAAAGAATTGAAAGACGTTAAAGAAGATTTACAtat                                                                                                       >  2:556398/1‑139 (MQ=255)
                                          aGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGa                                                                                                    <  1:367611/141‑1 (MQ=255)
                                           gATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGAt                                                                                                   <  1:269439/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:67393/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:451788/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:763971/141‑1 (MQ=255)
                                                   aTTGAAGTCATTAATTTTCAAAACGAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTa                                                                                           <  1:256434/141‑1 (MQ=255)
                                                      ggaGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACgg                                                                                        <  2:369236/139‑1 (MQ=255)
                                                      aaaGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACgg                                                                                        <  2:668903/140‑1 (MQ=255)
                                                         gTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACAAAATACG‑ACCATACTAAAGGGGAAAAAGTAAAAGTAGTGAAAGATGTTAAAGAAGATTCACATGGAGACGAAATTGACAGacg                                                                                     >  1:130210/1‑139 (MQ=255)
                                                                  tttCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAAAAGACACTAAGTAAG‑AGCATACTAAAGGGGAAAAAGTAAAAGAATTGAAAGATGTAAAAGAAGATTTACATGTACATGAAATTTACGGAACCTTATATcc                                                                            >  2:360918/1‑140 (MQ=255)
                                                                   ttCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATAATAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCAt                                                                           >  1:697793/1‑141 (MQ=255)
                                                                    tCAAAACTAAGTTAACTAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATa                                                                          <  1:422121/141‑1 (MQ=255)
                                                                    tCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACCGAAGCTTATATCATa                                                                          <  1:586894/141‑1 (MQ=255)
                                                                    tCAAAACAAAGGAAACAAGTTTAGAAGATATTGATAAAGCAGAAACTAAATAAG‑AAAAAAAAAAAGGGGAAAAAGAAAAAGTATTGAAAGATGTTAAAGAAGCTTTACATGTAGGTGAGATTTACGGTAGTTtaaaaaaaa                                                                          <  2:37327/141‑8 (MQ=255)
                                                                       aaaCTAAGGTAACGAGTTGAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGCATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAg                                                                       <  2:16324/141‑1 (MQ=255)
                                                                       aaaCTAAGGTAACGAGGTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAg                                                                       <  1:690873/141‑1 (MQ=255)
                                                                           ttaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATAGG‑ACCATACGAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTAGGGAAGCTTATATCATACAGaa‑aa                                                                  >  2:526253/3‑141 (MQ=255)
                                                                           tcaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTCTGGTTGCTTATACCATACTGatac                                                                   <  2:782270/139‑4 (MQ=255)
                                                                            aaGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGaa‑aaa                                                                 <  2:652837/141‑1 (MQ=255)
                                                                              ggTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑aaagg                                                               >  1:106837/1‑141 (MQ=255)
                                                                              ggTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑aaagg                                                               >  1:215187/1‑141 (MQ=255)
                                                                                gaaCGAGGTTAGAAGATAATGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGGGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAACTTACGGAAGCTTATATAATACAGAA‑aaaggta                                                             <  1:292605/140‑1 (MQ=255)
                                                                                  aCGAGTTTAGAAGATATTGATTAAGCACAAACTGAATATGCCCCCCCCCCAAGT‑GAAGAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑aaaggtaaa                                                           <  1:179239/141‑1 (MQ=255)
                                                                                  aCGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATATACGGAAGCTTATATCATACAGAA‑aaaggtaaa                                                           >  2:440769/1‑141 (MQ=255)
                                                                                  aCGAGGTTAGAAGATATTGATAAAGCAGGAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAGAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAAATTACGGAAGCTAATATGATACAGAA‑aaaggtaaa                                                           >  2:298530/1‑141 (MQ=255)
                                                                                     aGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAa‑aaaggaaaagga                                                        >  2:98745/1‑140 (MQ=255)
                                                                                     aGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑aaaggtaaaggt                                                        <  1:723831/141‑1 (MQ=255)
                                                                                       tttAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTAt                                                      >  2:67519/1‑141 (MQ=255)
                                                                                        ttAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTAtt                                                     >  2:647870/1‑141 (MQ=255)
                                                                                        ttAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTAtt                                                     >  2:574255/1‑141 (MQ=255)
                                                                                         tAGAAGATATTGATAAAGGAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTc                                                    <  2:131659/141‑1 (MQ=255)
                                                                                         tAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTc                                                    <  2:695319/141‑1 (MQ=255)
                                                                                           gaagaTATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCtt                                                  <  1:4419/141‑1 (MQ=255)
                                                                                             agaTATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGa                                                >  1:701869/1‑141 (MQ=255)
                                                                                             agaTATTGATAAAGCAGAAACCAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGa                                                <  1:68677/141‑1 (MQ=255)
                                                                                               atatTGATAAAGCAGAAACTAAATAAG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATa                                              <  1:232957/141‑1 (MQ=255)
                                                                                               atatTGAAAAAGCAGAAACGAAATATG‑AACAAAAAAAAAAAAAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATa                                              <  2:568505/141‑1 (MQ=255)
                                                                                                tatTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATCCTTGATaa                                             <  1:424149/141‑1 (MQ=255)
                                                                                                 atTGATAAAGCAGAACCTACATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAC‑AAAGGTAAAGGTATTCTTGATaaa                                            <  2:231532/141‑1 (MQ=255)
                                                                                                   tGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGATAAAGAAGATTTACATGTAGATGAAATTTACGGAAGATGAAATCATACAGAA‑CAAGGGAAAGGTATTGTTGATAaaga                                          >  2:122086/1‑141 (MQ=255)
                                                                                                     aTAAAGCAGAAACTAAATATT‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAaagaag                                        <  1:378186/141‑1 (MQ=255)
                                                                                                       aaaGCAGTAAACAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCAT‑CAGCACCTGGGCAAAGGTATTCTTGATAAAGAAGGt                                      <  1:189026/141‑1 (MQ=255)
                                                                                                          gCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACt                                   >  2:108986/1‑141 (MQ=255)
                                                                                                            aGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaa                                 >  2:668938/1‑141 (MQ=255)
                                                                                                             gAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                >  2:340847/1‑141 (MQ=255)
                                                                                                             gAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                >  2:523813/1‑141 (MQ=255)
                                                                                                             gAAACTAAATAGG‑AACATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                <  2:546521/141‑1 (MQ=255)
                                                                                                             aacaatAAATATG‑TAAATAATAAAGTGGAAAAAGTAAAAGTATTGCAAGATTTTAAAGAAGATTTACAAGTAGATGAAAGTTACGTAAGCTTAAAAAAAAAAAAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTaaa                                <  2:616223/136‑1 (MQ=255)
                                                                                                              aaaCTAAATATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAg                               <  1:394474/141‑1 (MQ=255)
                                                                                                              aaaCTAAATATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGT‑AAGGTATTCTTGATAAAGAAGGTACTAAAGt                              >  1:678280/1‑141 (MQ=255)
                                                                                                                aCTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTAGTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTa                             <  1:642760/141‑1 (MQ=255)
                                                                                                                aCTAAATATG‑AACAGACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTa                             <  2:572592/141‑1 (MQ=255)
                                                                                                                 cTAAATATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            >  1:518945/1‑141 (MQ=255)
                                                                                                                 cTAAAGATG‑ACCATACTAAAGTGGAAATAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            <  1:354527/141‑1 (MQ=255)
                                                                                                                 atAAATATG‑AACATACGAAAGTGGATATAGTAAAAGGATTGAGAGATCGTAAAGAAGATTTACATGTAGATGAAATTTTCGGAAGCTTAAAAAAAAAAAAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTaa                            <  2:275085/140‑1 (MQ=255)
                                                                                                                  tAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGAATTCTTGATAAAGAAGGTACTAAAGTAAt                           >  2:142455/1‑141 (MQ=255)
                                                                                                                   aaaTATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAAtt                          >  1:378558/1‑141 (MQ=255)
                                                                                                                    aaTATG‑ACCATACTAAAATGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAAATTTACATGTAGATGAAATTTACGGAAGCCTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTa                         <  2:702927/141‑1 (MQ=255)
                                                                                                                     atatG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAATCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaa                        <  2:597908/141‑1 (MQ=255)
                                                                                                                     atatG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaa                        <  1:300149/141‑1 (MQ=255)
                                                                                                                       atG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAACGTAATTAAAg                      >  2:424365/1‑141 (MQ=255)
                                                                                                                       aatcaCCATCCTAAAGTGGAAAAAGTAAAAGTATTCAAAGATGTTAAAGAAGATTTACATGTAGATGAAACTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTaaa                       <  1:159420/137‑1 (MQ=255)
                                                                                                                               tACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGaa               >  2:740710/1‑140 (MQ=255)
                                                                                                                                aCTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGAca              >  1:126228/1‑140 (MQ=255)
                                                                                                                                 ctcaaGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTa             <  1:335348/138‑1 (MQ=255)
                                                                                                                                      ggggAAATAATAAAAGCAATGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTAAAAAAAAAAAAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTc        <  2:202759/139‑1 (MQ=255)
                                                                                                                                      gTGGCAAAAGTAAAAGTATTGAAAGATGTTACAGAAGATTTACATGTAGATGAAAATTACGGAAGCTTATATCATACCGAA‑AAAGGTCAAGGTATTCTTGATAAAGAAGGTACTCAAATAATTAAAGGTAAGACTAAATTc        >  2:308964/1‑141 (MQ=255)
                                                                                                                                          aaaaaGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGCAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAGAGAAGGTACTAAAGTAATTAAAGGTACGACTAAATTCGCaa    >  2:159481/1‑141 (MQ=255)
                                                                                                                                          aaaaaGTAAAAGTATTGAAAGATGGTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCaa    <  1:225289/141‑1 (MQ=255)
                                                                                                                                            aaagtaaaagtaTTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAt  <  2:648286/141‑1 (MQ=255)
                                                                                                                                            aaaataaaagtaTTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAt  <  2:154054/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTGAAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACTAAATATG‑ACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTACATGTAGATGAAATTTACGGAAGCTTATATCATACAGAA‑AAAGGTAAAGGTATTCTTGATAAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAAT  >  USA300TCH1516_ALE/462078‑462357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑