Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,159,4260GA60.2% 78.4 / 132.2 113V330I (GTC→ATC) yheSputative ABC transporter ATP‑binding protein YheS
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base A (35/33);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 114 total aligned reads displayed.

TTACCCGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCTTAATTAAAACTATTGC  >  USA300TCH1516_ALE/2159289‑2159565
                                                                                                                                         |                                                                                                                                           
ttACCCGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATca                                                                                                                                          >  3:288446/1‑141 (MQ=255)
 tACCCGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTATATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGAcatcat                                                                                                                                         >  4:420768/1‑141 (MQ=255)
  aCCCGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTGGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTcatg                                                                                                                                        >  1:503774/1‑141 (MQ=255)
    ccGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTcatgca                                                                                                                                      >  3:288/1‑141 (MQ=255)
       tGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGt                                                                                                                                   <  1:380861/141‑1 (MQ=255)
        gCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTa                                                                                                                                  <  1:1031201/141‑1 (MQ=255)
        gCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTa                                                                                                                                  <  2:353371/141‑1 (MQ=255)
        gCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTa                                                                                                                                  <  2:423936/141‑1 (MQ=255)
        gCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTa                                                                                                                                  <  1:615383/141‑1 (MQ=255)
             aacaaGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAATCCACTGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTaaaaaa                                                                                                                             <  2:146757/141‑1 (MQ=255)
             aacaaGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTaaaaaa                                                                                                                             <  4:58253/141‑1 (MQ=255)
                   tGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAATCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGa                                                                                                                       <  3:139624/141‑1 (MQ=255)
                   gggAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGa                                                                                                                       <  1:149920/140‑1 (MQ=255)
                     gAATGGCAAAAAGTAGACGTAAGATTTTAGTAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGaaa                                                                                                                     >  1:252070/1‑141 (MQ=255)
                      aaTGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAAt                                                                                                                    >  4:86182/1‑141 (MQ=255)
                         ggCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCgg                                                                                                                 >  1:896260/1‑141 (MQ=255)
                         ggCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCgg                                                                                                                 >  4:13628/1‑141 (MQ=255)
                             aaaaGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTAt                                                                                                             >  3:433679/1‑141 (MQ=255)
                               aaGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCa                                                                                                           >  2:402665/1‑141 (MQ=255)
                                   aGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTTCAAATATTCAATTTGGCTTTGACCGTAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACt                                                                                                       <  1:238484/141‑1 (MQ=255)
                                   aGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACt                                                                                                       >  2:53762/1‑141 (MQ=255)
                                   aGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACt                                                                                                       >  1:579831/1‑141 (MQ=255)
                                    gACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTg                                                                                                      >  3:249101/1‑141 (MQ=255)
                                     aCGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGc                                                                                                     >  3:306798/1‑141 (MQ=255)
                                      ccgaaagttttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCa                                                                                                    <  1:162375/134‑1 (MQ=255)
                                      cGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCa                                                                                                    >  1:40731/1‑141 (MQ=255)
                                       gTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCaa                                                                                                   <  4:381465/141‑1 (MQ=255)
                                       gTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCaa                                                                                                   <  1:79539/141‑1 (MQ=255)
                                       gTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCaa                                                                                                   <  1:766038/141‑1 (MQ=255)
                                           gATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTAc                                                                                               <  1:457580/141‑1 (MQ=255)
                                            aTTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTAcc                                                                                              <  1:670277/141‑1 (MQ=255)
                                             ttttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCa                                                                                             <  2:442364/141‑1 (MQ=255)
                                             ttttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCa                                                                                             <  1:271510/141‑1 (MQ=255)
                                             ttttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCa                                                                                             <  4:288446/141‑1 (MQ=255)
                                             ttttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCa                                                                                             <  1:376203/141‑1 (MQ=255)
                                              tttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCaa                                                                                            >  2:806542/1‑141 (MQ=255)
                                              tttAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCaa                                                                                            >  2:512754/1‑141 (MQ=255)
                                                tAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAAc                                                                                          >  2:234570/1‑141 (MQ=255)
                                                 aGAAAAAATGGAACGCATTGATAACCCAATGTTAGATGCCAAAAGTGCAAATATTCAATATGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCATACTGCCATTACCAAAcc                                                                                         >  2:55242/1‑141 (MQ=255)
                                                  gAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCt                                                                                        >  3:437421/1‑141 (MQ=255)
                                                   aaaaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTa                                                                                       >  1:997630/1‑141 (MQ=255)
                                                   aaaaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTa                                                                                       >  1:369719/1‑141 (MQ=255)
                                                    aaaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTAt                                                                                      <  3:98134/141‑1 (MQ=255)
                                                    aaaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTAt                                                                                      <  3:98131/141‑1 (MQ=255)
                                                     aaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATg                                                                                     <  2:135587/141‑1 (MQ=255)
                                                     aaaaTGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTACAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATg                                                                                     <  1:31806/141‑1 (MQ=255)
                                                     aaaaTGGAACGCATTGATAAACCAATGTGAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATg                                                                                     <  1:887191/141‑1 (MQ=255)
                                                         tGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGta                                                                                 <  1:361000/141‑1 (MQ=255)
                                                            aaCGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAAGGTAGAAATCGGTTATCAAAATGCAATTACCAAACCTATGAGtataa                                                                              >  2:655268/1‑140 (MQ=255)
                                                            aaCGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATag                                                                              >  2:552186/1‑141 (MQ=255)
                                                             aCGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATaga                                                                             <  1:887376/141‑1 (MQ=255)
                                                             aCGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATaga                                                                             >  2:355533/1‑141 (MQ=255)
                                                               gCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAgg                                                                           <  2:960469/141‑1 (MQ=255)
                                                                  ttGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGtct                                                                        <  2:701970/141‑1 (MQ=255)
                                                                  ttGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGtct                                                                        <  3:72742/141‑1 (MQ=255)
                                                                   tGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGtctc                                                                       <  2:853798/141‑1 (MQ=255)
                                                                     aTAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTa                                                                     <  2:163248/141‑1 (MQ=255)
                                                                         aCCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAgg                                                                 <  4:451507/141‑1 (MQ=255)
                                                                         aCCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACAAAAAAAAAAAAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAgg                                                                 <  2:156332/141‑1 (MQ=255)
                                                                            aaTGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGa                                                              <  1:212499/141‑1 (MQ=255)
                                                                              tGTTAGATGCCAAAAGTGCAAATATTCAATTTGGTTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATc                                                            >  1:467617/1‑141 (MQ=255)
                                                                                   gATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCAtata                                                       >  2:757325/1‑141 (MQ=255)
                                                                                    aTGCCGAAAGTGCAAATATTCAATTTGGCTGTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTATAAAGGCGATCATATAg                                                      <  1:920315/141‑1 (MQ=255)
                                                                                    aTGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAg                                                      >  1:770051/1‑141 (MQ=255)
                                                                                    aTGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAg                                                      <  2:579831/141‑1 (MQ=255)
                                                                                    aTGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAg                                                      >  2:153492/1‑141 (MQ=255)
                                                                                     tGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGc                                                     <  2:624991/141‑1 (MQ=255)
                                                                                      gCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCa                                                    >  2:394381/1‑141 (MQ=255)
                                                                                           aaGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCAt                                               >  4:217390/1‑141 (MQ=255)
                                                                                            aGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCAtt                                              <  1:399139/141‑1 (MQ=255)
                                                                                             gTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTg                                             >  3:448937/1‑141 (MQ=255)
                                                                                             gTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTg                                             <  2:907198/141‑1 (MQ=255)
                                                                                               gCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTGATCAAACCGAATGAACCTAACATAGGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTggg                                           >  1:731074/1‑141 (MQ=255)
                                                                                                 aaaTATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGcc                                         <  1:716494/141‑1 (MQ=255)
                                                                                                 aaaTATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGcc                                         <  1:220330/141‑1 (MQ=255)
                                                                                                  aaTATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCa                                        <  1:1020679/141‑1 (MQ=255)
                                                                                                  aaTATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGGATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCa                                        <  1:901244/141‑1 (MQ=255)
                                                                                                  aaTATTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCa                                        >  4:209426/1‑141 (MQ=255)
                                                                                                     atTCAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAAt                                     >  2:969316/1‑141 (MQ=255)
                                                                                                        cAATTTGGCTTTGACCGGAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGt                                  >  2:10587/1‑141 (MQ=255)
                                                                                                          aTTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTAt                                >  2:419334/1‑141 (MQ=255)
                                                                                                            ttGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTg                              <  1:1035007/141‑1 (MQ=255)
                                                                                                                cTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGaaa                          >  2:434571/1‑141 (MQ=255)
                                                                                                                 tttGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGaaaa                         <  2:150699/141‑1 (MQ=255)
                                                                                                                   tGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATc                       >  1:601638/1‑141 (MQ=255)
                                                                                                                       cGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGAcc                   <  2:503774/141‑1 (MQ=255)
                                                                                                                        ggAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCt                  <  1:232835/141‑1 (MQ=255)
                                                                                                                        ggAATACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAAACTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCt                  <  4:323708/141‑1 (MQ=255)
                                                                                                                          aaTACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCtta                >  1:1012325/1‑141 (MQ=255)
                                                                                                                          aaTACAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCtta                >  1:101743/1‑141 (MQ=255)
                                                                                                                             aCAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaatt             >  2:797369/1‑141 (MQ=255)
                                                                                                                             aCAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaatt             >  2:1031446/1‑141 (MQ=255)
                                                                                                                             aCAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaatt             >  1:106225/1‑141 (MQ=255)
                                                                                                                             aCAGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTAACCAACCTATGAGTCTAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaatt             <  1:261432/141‑1 (MQ=255)
                                                                                                                               aGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaattaa           <  1:831501/141‑1 (MQ=255)
                                                                                                                               aGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaattaa           <  4:398398/141‑1 (MQ=255)
                                                                                                                               aGGTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCttaattaa           <  2:849212/141‑1 (MQ=255)
                                                                                                                                 gTAATGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATATAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCTTAATTaaaa         >  4:216754/1‑141 (MQ=255)
                                                                                                                                   aaTGACATCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCTTAATTAAAACt       <  2:497297/141‑1 (MQ=255)
                                                                                                                                        cGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCTTAATTAAAACTATTGc  >  1:813973/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TTACCCGTGCTTCAACAAGTGGAATGGCAAAAAGTAGACGTAAGATTTTAGAAAAAATGGAACGCATTGATAAACCAATGTTAGATGCCAAAAGTGCAAATATTCAATTTGGCTTTGACCGGAATACAGGTAATGACGTCATGCATGTAAAAAATTTAGAAATCGGTTATCAAACTGCAATTACCAAACCTATGAGTATAGAGGTCTCTAAAGGCGATCATATAGCAATCATTGGGCCAAATGGTATTGGAAAATCGACCTTAATTAAAACTATTGC  >  USA300TCH1516_ALE/2159289‑2159565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑