Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 656,852 T→C 5.9% T31T (ACT→ACC)  USA300HOU_RS03140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE656,8520TC 5.9% 180.5 / 3.4 69T31T (ACT→ACC) USA300HOU_RS03140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (44/20);  new base C (2/2);  total (47/22)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

GTTCTTCAAATAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGATTTGCAG  >  USA300TCH1516_ALE/656714‑656991
                                                                                                                                          |                                                                                                                                           
gTTCTTCAAATAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTg                                                                                                                                           >  1:393065/1‑141 (MQ=255)
gTTCTTCAAATAAAAAATAATTTGAATAAGGGGGAAGTGATAAACAGTGCTTTGGGAATCTCGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACtag                                                                                                                                           >  2:297713/1‑139 (MQ=255)
      cAAATAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACCTGGATTGc                                                                                                                                     >  1:792758/1‑141 (MQ=255)
      cAAATAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATCGTTGATTTAGTAAGTACCTGGATTGc                                                                                                                                     >  1:959004/1‑141 (MQ=255)
       aaaTAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTTCGAATCGAGAGTCATTAATCAAAACCCTAAATATAGAAGTATTAAATACAATAGTGAGTATTTCATGGTTGAGTTACTGAGTATTAGGATTGCt                                                                                                                                    >  2:215315/1‑141 (MQ=255)
       aaaTAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCt                                                                                                                                    >  2:40524/1‑141 (MQ=255)
       aaaTAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCt                                                                                                                                    >  1:358697/1‑141 (MQ=255)
       aaaTAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATAATATAATGAATATTTAATGGTTGATTTAGTAAGTACTTGGATTgat                                                                                                                                    >  2:565168/1‑139 (MQ=255)
        aaTAAAAAATAATTTGAATAAGGTGGAGGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATAAAAACCCTAAATATAGAATTATTAAATATAATAATGATTATTTAATTCGTGATTTAGTAAGAAATTGgattgatt                                                                                                                                   >  2:803712/1‑141 (MQ=18)
         aTAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTa                                                                                                                                  <  1:458375/141‑1 (MQ=255)
          tAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTAt                                                                                                                                 <  2:357007/141‑1 (MQ=255)
             aaaaTAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTGAGTAAGTACTTGGATTGCTTAtttt                                                                                                                              >  1:417907/1‑141 (MQ=255)
             aaaaTAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACACTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTAtttt                                                                                                                              >  2:969845/1‑141 (MQ=255)
             aaaaCAATTTGAATGGGGTGGAAGTTGTAAACAGTGCTTTGCGAATCTTGAGACATTAAGCAAATCCCTAAATACAAAATTATTAAATACAATAATGAAGATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTAtttt                                                                                                                              <  2:166867/141‑1 (MQ=21)
                ataatTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTg                                                                                                                           >  1:714573/1‑141 (MQ=255)
                     ttGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCAt                                                                                                                      <  1:198372/141‑1 (MQ=255)
                       taaTAAGGTGGAAGAGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTA‑TTTTTGCCCATGAt                                                                                                                   <  1:837341/140‑1 (MQ=255)
                       gAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGa                                                                                                                    <  2:406221/141‑1 (MQ=255)
                       gAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTA‑TTTTTGCCCATGAt                                                                                                                   <  1:837023/141‑1 (MQ=255)
                        aagaaGGTGCAAGTGATAAACAGTGCTTTGCGAATCTAGATTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGAt                                                                                                                   <  1:901085/138‑1 (MQ=255)
                                     tGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCAATGATTAATTGGTTTAtt                                                                                                      >  2:428959/1‑141 (MQ=255)
                                       aTAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTcc                                                                                                    >  1:160960/1‑141 (MQ=255)
                                        tAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTccc                                                                                                   <  1:425534/141‑1 (MQ=255)
                                              gTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAg                                                                                             >  2:944279/1‑141 (MQ=255)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTTTTTTTTTTTAATTGGTTTATTCCCATAAAGTAcg                                                                                         >  2:333299/1‑141 (MQ=25)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCTAGAAGTAcg                                                                                         >  2:1075880/1‑141 (MQ=255)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTAcg                                                                                         >  1:75008/1‑141 (MQ=255)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTAcg                                                                                         >  1:881608/1‑141 (MQ=255)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTAcg                                                                                         <  1:432461/141‑1 (MQ=255)
                                                  tttGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTAcg                                                                                         >  1:947649/1‑141 (MQ=255)
                                                          tCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAAtt                                                                                 <  1:1021848/141‑1 (MQ=255)
                                                          tCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAAtt                                                                                 <  2:34961/141‑1 (MQ=255)
                                                               agTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATtagtag                                                                            >  1:837204/1‑141 (MQ=255)
                                                               agTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATtagtag                                                                            >  1:881407/1‑141 (MQ=255)
                                                               agTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACGTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCGAAAAGTACGCGCACATtggtag                                                                            >  1:178527/1‑141 (MQ=255)
                                                                gTCTTTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAGTATAGGCTCGTGGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACCCGAAAATTAGTaga                                                                           <  1:577624/141‑1 (MQ=21)
                                                                  cATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAgaga                                                                         >  2:891314/1‑141 (MQ=255)
                                                                  cATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAgaga                                                                         >  1:1060297/1‑141 (MQ=255)
                                                                            aaCCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTTTTTTTTTTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGaa                                                               >  2:113055/1‑141 (MQ=21)
                                                                            aaCCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTTCTTTTGTTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGaa                                                               >  2:366664/1‑141 (MQ=21)
                                                                                 tAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAGTTGGTTTATGCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGttt                                                          <  1:428959/141‑1 (MQ=255)
                                                                                 tAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGttt                                                          >  1:198932/1‑141 (MQ=255)
                                                                                         gAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTg                                                  >  2:145190/1‑141 (MQ=255)
                                                                                                     aCAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCACACCCGCTa                                      >  2:679168/1‑141 (MQ=255)
                                                                                                     aCAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTa                                      >  1:532343/1‑141 (MQ=255)
                                                                                                       aataatGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTaaa                                    >  1:1030714/1‑141 (MQ=255)
                                                                                                        ataatGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTaaaa                                   >  1:181786/1‑141 (MQ=255)
                                                                                                           atGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaata                                >  1:1026531/1‑141 (MQ=255)
                                                                                                             gAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCGTGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaataat                              >  1:1000446/1‑141 (MQ=255)
                                                                                                             gAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaataat                              >  1:926029/1‑141 (MQ=255)
                                                                                                               atatTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATAc                            >  2:248114/1‑141 (MQ=255)
                                                                                                               atatTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATAc                            >  2:796771/1‑141 (MQ=255)
                                                                                                                    tCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCt                       >  1:274588/1‑141 (MQ=255)
                                                                                                                        ggTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGcc                   >  1:178939/1‑141 (MQ=255)
                                                                                                                         gTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCt                  >  2:250417/1‑141 (MQ=255)
                                                                                                                           tGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTAGCCTGt                >  1:654682/1‑141 (MQ=255)
                                                                                                                            gATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGCGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGtt               >  2:664705/1‑141 (MQ=255)
                                                                                                                             aTTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTg              <  1:796771/141‑1 (MQ=255)
                                                                                                                             aTTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTg              <  1:248114/141‑1 (MQ=255)
                                                                                                                             aTTCAGTAAGTACTTGGATTGTTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAATAATTTGAAAGCTTAAATAGTGTCAAAGCCGCTAAAAATAATACTTTCTGGCCTGTTg              <  2:519772/141‑1 (MQ=255)
                                                                                                                              tttAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc             >  2:745169/1‑141 (MQ=255)
                                                                                                                              tttAGGAAGTACTTGAGTTGCTTATTTTTTGACCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc             <  2:111335/141‑1 (MQ=255)
                                                                                                                                 agtaagtaCCTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAgg          <  2:792758/141‑1 (MQ=255)
                                                                                                                                 agtaagtaCCTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAgg          <  2:959004/141‑1 (MQ=255)
                                                                                                                                  gtaagtaCTTGGATTGCTTATTTTTTGCCTATTATTAATTGGTTTATTCCCAAAAAGTACGCGACAATTAGTAGAGAAGAATTTGATAGTTTAAATATTGTCAAACCCGGTAAAATTAATACTTTGTGCACTGTTgtctgt         >  2:1068915/1‑136 (MQ=255)
                                                                                                                                  gtaagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGa         >  1:915230/1‑141 (MQ=255)
                                                                                                                                   taagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATGGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGAt        <  1:420676/141‑1 (MQ=255)
                                                                                                                                   gaagtaCTTGGATTGCTTATTTTTTGCCCATGATCAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGAt        <  1:319468/140‑1 (MQ=255)
                                                                                                                                         cTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGATTTGCAg  >  1:621150/1‑141 (MQ=255)
                                                                                                                                         cTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGAATTTGCAg  >  2:457673/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GTTCTTCAAATAAAAAATAATTTGAATAAGGTGGAAGTGATAAACAGTGCTTTGCGAATCTAGAGTCATTAATCAAAACCCTAAATATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGCAGGATTTGCAG  >  USA300TCH1516_ALE/656714‑656991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑