Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,684,834 G→T 7.3% S52Y (TCT→TAT)  USA300HOU_RS08395 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,684,8340GT 7.3% 193.9 / 3.8 67S52Y (TCT→TAT) USA300HOU_RS08395hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/35);  new base T (2/3);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

ATAATTCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTAAAAAA  >  USA300TCH1516_ALE/1684694‑1684974
                                                                                                                                            |                                                                                                                                            
ataatTCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTag                                                                                                                                              >  2:159015/1‑141 (MQ=255)
 aaatCCAACTACAACTAAAATAACGCCAAAGGAAAACAATAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTaga                                                                                                                                             <  2:386482/140‑1 (MQ=255)
  aatacaaatacaACTAATATAACGCCAATTGAGAATAATAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAAGCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTagag                                                                                                                                            <  2:46443/141‑1 (MQ=255)
  aatTCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTagag                                                                                                                                            <  1:444325/141‑1 (MQ=255)
  aatTCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTagag                                                                                                                                            <  1:403483/141‑1 (MQ=255)
  aaatCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTagag                                                                                                                                            <  2:435157/138‑1 (MQ=255)
           aCAACTATAATAACGCCAATTGAGAATATTTACATCGCCCGGCTCTTCACTTTTGCCTATTGTTTAAATCCCAAAAATAAAATAAGTAAGTATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGa                                                                                                                                   <  2:1062185/141‑1 (MQ=255)
             aaCTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGata                                                                                                                                 <  1:545624/141‑1 (MQ=255)
                    ataACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAAt                                                                                                                          <  2:896037/141‑1 (MQ=255)
                    ataACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTGAGTGGAAAGTTGATATACaaag                                                                                                                          >  1:469294/1‑140 (MQ=255)
                    aaaaCGCCAATAGAGAATATTAACAAACAATGCATATTGACATTTCCTGGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAAt                                                                                                                          <  2:185508/139‑1 (MQ=255)
                        cGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTa                                                                                                                      <  2:598487/141‑1 (MQ=255)
                          ccAATTGAGAATATTTAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAg                                                                                                                    >  1:821615/1‑141 (MQ=255)
                          ccAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTATAGTAAAGTTGATATACAAATCCTAAg                                                                                                                    >  2:398018/1‑141 (MQ=255)
                          ccAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTATAGTAAAGTTGATATACAAATCCTAAg                                                                                                                    >  2:398165/1‑141 (MQ=255)
                          ccAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAg                                                                                                                    >  1:71457/1‑141 (MQ=255)
                              ttGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATg                                                                                                                <  1:959629/141‑1 (MQ=255)
                              ttGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAACAATg                                                                                                                >  2:33759/1‑141 (MQ=255)
                                gagaATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTc                                                                                                              <  2:84532/141‑1 (MQ=255)
                                 agaATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCa                                                                                                             <  2:858259/141‑1 (MQ=255)
                                  gaATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGCAATCCCAAAAATAAAATAAGTAATGATAAGGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCaa                                                                                                            >  2:22129/1‑141 (MQ=255)
                                  gaATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGCTTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCaa                                                                                                            >  1:200968/1‑141 (MQ=255)
                                  gaATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCaa                                                                                                            >  2:910128/1‑141 (MQ=255)
                                   aaTATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAATTGATATACAAATCCTAAGAATGTCaaa                                                                                                           >  2:279760/1‑141 (MQ=255)
                                       ttAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAgg                                                                                                       <  1:217088/141‑1 (MQ=255)
                                       ttAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAgg                                                                                                       <  1:28444/141‑1 (MQ=255)
                                        tAATATAGAACGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGAGAATGTGGCGATAATACCAGCTGCATTGATTTTTGTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGt                                                                                                      <  1:271162/141‑1 (MQ=255)
                                        tAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAGTCCCCACAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGt                                                                                                      >  1:758882/1‑141 (MQ=255)
                                        tAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCCGAATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGt                                                                                                      >  1:305847/1‑141 (MQ=255)
                                         aaGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGtt                                                                                                     >  1:779048/1‑141 (MQ=255)
                                         aaGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGAAAAGTTGAGATACAAGTCCTACGAATGTCAAACAGGtt                                                                                                     >  1:202052/1‑141 (MQ=255)
                                                aaTGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTa                                                                                              >  1:327493/1‑141 (MQ=255)
                                                        ttGACATTTCCTTGGAATAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTaaaa                                                                                      >  1:566025/1‑141 (MQ=255)
                                                            cATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTGAAAATAg                                                                                  >  2:884612/1‑141 (MQ=255)
                                                             aTTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAgg                                                                                 <  2:701594/141‑1 (MQ=255)
                                                                tCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATTCCAGCAGAATTGATTTTTTTAGAGGAAAGTTGATGTGCAAATCGTAGGAGTGTCAAACAGGTTAATATTAACGCAAACAGAGGTTg                                                                              >  1:440325/1‑141 (MQ=255)
                                                                tCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCATAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGATg                                                                              >  1:789309/1‑141 (MQ=255)
                                                                tCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATAAAAATACTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAAGTTg                                                                              >  2:46806/1‑141 (MQ=255)
                                                                 ccTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACCGATACTGTGGGTGTCAGACAGGTTAATATTAACGTAAAAATAGGTTGa                                                                             >  1:574119/1‑141 (MQ=255)
                                                                       aTTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAAtt                                                                       <  2:979089/141‑1 (MQ=255)
                                                                             aaTCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTg                                                                 >  1:713054/1‑141 (MQ=255)
                                                                              ataacAAACATAAACTATATAATGATAATGTACCGATCATACCAGCTGCTTTGATTTTTTTAGAGCAGAGTGGATACACAAATCCTAAGAATGTCAAACAAGTTAATATTCACGTAAAAATAGGTTGTACAATTATATTGc                                                                <  2:611087/137‑1 (MQ=255)
                                                                              aTCCCAAAAATAAGATAAGTAATGATACTGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGc                                                                <  2:560762/141‑1 (MQ=255)
                                                                              aTCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGc                                                                <  2:793830/141‑1 (MQ=255)
                                                                              aTCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGc                                                                <  1:415324/141‑1 (MQ=255)
                                                                               acccAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCt                                                               <  2:741412/140‑1 (MQ=255)
                                                                                cccAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTATAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTa                                                              <  1:398018/141‑1 (MQ=255)
                                                                                cccAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTATAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTa                                                              <  1:398165/141‑1 (MQ=255)
                                                                                cccAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATGGGTTGAACAATTATATTGCTa                                                              <  2:71457/141‑1 (MQ=255)
                                                                                cccAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTa                                                              <  2:821615/141‑1 (MQ=255)
                                                                                   aaaaaaaaaaTAAGTCATGACAATGTTGGAATAATACCCGCTTCATTGATATCTTTATAGTAAACTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACt                                                           <  2:719744/141‑1 (MQ=255)
                                                                                   aaaaaTAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACt                                                           <  2:543719/141‑1 (MQ=255)
                                                                                   aaaaaTAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACt                                                           <  1:356605/141‑1 (MQ=255)
                                                                                                 tAAGGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCACCCCAAGt                                             <  2:595555/141‑1 (MQ=255)
                                                                                                  aaTGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTa                                            <  1:46058/141‑1 (MQ=255)
                                                                                                  aaTGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTa                                            <  1:242260/141‑1 (MQ=255)
                                                                                                  aaTGATAAGGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCGAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTa                                            <  2:362418/141‑1 (MQ=255)
                                                                                                          tgtgGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATCTTGCTAACTTGTTCAACCCAAGTATCTCCAGc                                    <  2:783041/141‑1 (MQ=255)
                                                                                                          tgtgGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGc                                    <  1:1071098/141‑1 (MQ=255)
                                                                                                          tgtgGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGc                                    <  2:90704/141‑1 (MQ=255)
                                                                                                          tggggCGATAATACCATCTGCATTGATTTTGTTAGATTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGc                                    <  2:440325/138‑1 (MQ=255)
                                                                                                                  taataCCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACTATTGTATTGCTAACTTGTCCAACCCAAGTATCTCCAGCCGTTGAtt                            >  1:571821/1‑141 (MQ=255)
                                                                                                                  taataCCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCGCAGTATCTCCAGCCGCTGAtg                            >  1:185019/1‑140 (MQ=255)
                                                                                                                    ataCCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTcc                          >  1:634323/1‑141 (MQ=255)
                                                                                                                       ccAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATGCTAGGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGGTGAACAATAATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCGGTTGATTCCacg                       >  1:211947/1‑139 (MQ=255)
                                                                                                                           cTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCACGGATGGTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAg                   <  1:753520/141‑1 (MQ=255)
                                                                                                                               aTTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAAGAATTATAGTTCTAACGTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGt               <  2:809461/141‑1 (MQ=255)
                                                                                                                               aTTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGt               >  1:994282/1‑141 (MQ=255)
                                                                                                                                      tttttAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTGTCTCCAGCCGTTGATTCCAAGATAGTCGTCATTTGt        <  1:810331/141‑1 (MQ=255)
                                                                                                                                       ttttAGAGTCAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTa       <  1:160136/141‑1 (MQ=255)
                                                                                                                                         ttAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAACAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTaaa     >  1:1084654/1‑141 (MQ=255)
                                                                                                                                         ttAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTaaa     <  2:713054/141‑1 (MQ=255)
                                                                                                                                         ttAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTaaa     >  1:628390/1‑141 (MQ=255)
                                                                                                                                            gagTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTaaaaaa  >  1:678432/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATAATTCAATTACAACTAATATAACGCCAATTGAGAATATTAAGATAGAATGCATATTGACATTTCCTTGGATTAGAAATCCCAAAAATAAAATAAGTAATGATAATGTGGCGATAATACCAGCTGCATTGATTTTTTTAGAGTAAAGTTGATATACAAATCCTAAGAATGTCAAACAGGTTAATATTAACGTAAAAATAGGTTGAACAATTATATTGCTAACTTGTTCAACCCAAGTATCTCCAGCCGTTGATTCCAAGATAGTTGTCATTTGTAAAAAA  >  USA300TCH1516_ALE/1684694‑1684974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑