BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,899,742	A→G	11.3%	N421N (AAT→AAC)	USA300HOU_RS09405 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,899,742	0	A	G	11.3%	263.5 / 22.6	105	N421N (AAT→AAC)	USA300HOU_RS09405	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (39/54); new base G (8/4); total (47/58)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 109 total aligned reads displayed.

TTTCGTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATCATG  >  USA300TCH1516_ALE/1899602‑1899882

tttCGTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTa                                                                                                                                              <  2:576569/141‑1 (MQ=255)

   cGTAGTTTATTAACTCCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTAttt                                                                                                                                           <  2:623730/141‑1 (MQ=255)

   cGTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGttt                                                                                                                                           <  2:1085019/141‑1 (MQ=255)

     tAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTc                                                                                                                                         <  1:1104767/141‑1 (MQ=255)

     tAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTc                                                                                                                                         <  2:649591/141‑1 (MQ=255)

             taACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTc                                                                                                                                 <  2:656894/141‑1 (MQ=255)

                cacCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCtaa                                                                                                                              >  2:1053146/1‑141 (MQ=255)

                    gTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCtaattaa                                                                                                                          <  1:1080087/141‑1 (MQ=255)

                    gTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCtaattaa                                                                                                                          <  1:130761/141‑1 (MQ=255)

                       tGTCATTTGACTTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTg                                                                                                                       <  2:715404/141‑1 (MQ=255)

                        ttCATTTGACCTATCGCAATCATTTTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGa                                                                                                                      <  2:247747/140‑1 (MQ=255)

                        ttCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGa                                                                                                                      <  2:107011/140‑1 (MQ=255)

                        gTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGa                                                                                                                      >  2:532521/1‑141 (MQ=255)

                          cATTTGGCCTATCGCAATCATTTTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGatc                                                                                                                    <  2:98245/141‑1 (MQ=255)

                          cATTTGACCTATCGCAAGCATATTTGTTAATGCTTCAAACCTTGGACTAATTACTTGATTGGTTTCCGCAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGgtc                                                                                                                    <  2:248026/141‑1 (MQ=255)

                           aTTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGatca                                                                                                                   <  2:1046611/141‑1 (MQ=255)

                           aTTTAACCTATCTCAATCATATTTTTTAATGTTCCAAATCTTGGACTCATAACTTGATTGTTTTCCTGAATGATTTGTATGCCTCCTATTGCGTGTGCTTTTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGatca                                                                                                                   <  2:233881/141‑1 (MQ=255)

                             ttGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGatcatc                                                                                                                 >  1:211228/1‑141 (MQ=255)

                             ttGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTGGTAGAATTTGTCTATTTTCAAGATTTCTAATTAATTGatcatc                                                                                                                 >  1:854045/1‑141 (MQ=255)

                                  cTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGAt                                                                                                            >  2:71655/1‑141 (MQ=255)

                                     tCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATGGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTGATTAATTGATCAGCTTGATCCa                                                                                                         >  1:1612/1‑141 (MQ=255)

                                      cGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTTCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCACGATTTCTATTTAATTGCTCCTCTTGATCCat                                                                                                        >  2:292553/1‑140 (MQ=255)

                                      cGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGCACCaa                                                                                                        >  2:540662/1‑141 (MQ=255)

                                       gCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAAt                                                                                                       <  1:842297/141‑1 (MQ=255)

                                         aaTCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCGTCTTGATCCAATTc                                                                                                     >  1:38987/1‑141 (MQ=255)

                                          aTCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCa                                                                                                    >  2:634959/1‑141 (MQ=255)

                                              tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCCTCTTGATCCAATTCATTTa                                                                                                <  2:535011/141‑1 (MQ=255)

                                              tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTa                                                                                                <  2:807006/141‑1 (MQ=255)

                                              tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTa                                                                                                <  1:766362/141‑1 (MQ=255)

                                                   gTTAATGTTCCAACCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGa                                                                                           <  1:429942/141‑1 (MQ=255)

                                                    ttAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAc                                                                                          >  2:852700/1‑141 (MQ=255)

                                                          ttCCAATCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTg                                                                                    <  1:939250/141‑1 (MQ=255)

                                                          ttCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTg                                                                                    >  1:886031/1‑141 (MQ=255)

                                                            ccAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACATTTTGCa                                                                                  <  1:696571/141‑1 (MQ=255)

                                                               aaCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCt                                                                               >  1:605556/1‑141 (MQ=255)

                                                                aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTAGATTGGTGTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTg                                                                              >  1:1087468/1‑141 (MQ=255)

                                                                aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCtt                                                                              >  2:536223/1‑140 (MQ=255)

                                                                aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTg                                                                              >  1:1052040/1‑141 (MQ=255)

                                                                aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAAAGACTTTTTGCATCTg                                                                              >  2:30508/1‑141 (MQ=255)

                                                                 ccTTGGACTAATAACTTGATTGGTATCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGt                                                                             >  1:706963/1‑141 (MQ=255)

                                                                   ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGGTCGTCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGtag                                                                           >  2:564798/1‑139 (MQ=255)

                                                                   ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGCCTTTTTGCAcacacat                                                                           >  2:542968/1‑135 (MQ=255)

                                                                      gACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTctctt                                                                        <  2:403493/141‑5 (MQ=255)

                                                                       aCTAAGAACTTGATTGGTTTCCTGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATGTTTCTATTTTCAATATTTCTAATTAATGGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGtt                                                                       <  1:1055253/141‑1 (MQ=255)

                                                                        aatataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTa                                                                      >  2:678199/4‑141 (MQ=255)

                                                                         taataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTaa                                                                     <  1:1055427/141‑1 (MQ=255)

                                                                          aataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAAt                                                                    <  1:939366/141‑1 (MQ=255)

                                                                          aataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAAATGGTCATTTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAAt                                                                    <  1:260440/141‑1 (MQ=255)

                                                                           ataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATg                                                                   <  2:494702/141‑1 (MQ=255)

                                                                           ataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTGTTGCACCTGTCGCGTTAATg                                                                   >  2:584265/1‑141 (MQ=255)

                                                                             aaCTTGATTGTTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGac                                                                 <  1:287992/141‑1 (MQ=255)

                                                                               cTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGacaa                                                               <  1:682103/141‑1 (MQ=255)

                                                                                ttGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGacaac                                                              <  1:1101062/141‑1 (MQ=255)

                                                                                  taTTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACAt                                                            <  2:950379/140‑1 (MQ=255)

                                                                                  gtttGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACAt                                                            <  1:903230/139‑1 (MQ=255)

                                                                                     tGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGtt                                                                       >  1:984835/1‑127 (MQ=255)

                                                                                     tGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACCTtat                                                         >  1:334622/1‑141 (MQ=255)

                                                                                      ggTTTCCGGAATGATTTGTTTGCCTCCCATTGCGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATtata                                                        <  2:143449/141‑1 (MQ=255)

                                                                                      ggTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGCTCATCTTGATCCAATTCATTTAAATGACTTTTTGCACGTGTCGCGTTAATGacaacagaatt                                                        >  1:241049/1‑136 (MQ=255)

                                                                                      ggTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTTTTTTTTTTTCGTTAATGAGAACATtata                                                        >  2:45798/1‑141 (MQ=255)

                                                                                          tCCGGAATGATTTGTATGCCTCCTATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATg                                                    <  1:70825/141‑1 (MQ=255)

                                                                                                aTGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACt                                              <  2:1028257/141‑1 (MQ=255)

                                                                                                 tGATTTGTATGGCTTCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTg                                             <  2:324857/141‑1 (MQ=255)

                                                                                                  gATTTGTTTGCCTCCCATTTGGTGTATTTGTACAATTTGTCTATTTTCATGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGa                                            <  2:720211/141‑1 (MQ=255)

                                                                                                  gATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGa                                            <  1:1024174/141‑1 (MQ=255)

                                                                                                  gATGTGTATGCCTCTCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGa                                            <  1:1024277/141‑1 (MQ=255)

                                                                                                   aTTTGTATGCCTCCCATTGGGCGTGCTTGTACACTTGGTCTAGTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGCTAATGACAACATTATATATGTCTACTgct                                           >  2:182717/1‑139 (MQ=255)

                                                                                                      tGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTGCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATTACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCt                                        <  2:430299/141‑1 (MQ=255)

                                                                                                             cTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGtttt                                 >  2:639857/1‑141 (MQ=255)

                                                                                                             cTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGtttt                                 >  1:785685/1‑141 (MQ=255)

                                                                                                                 cATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCACTTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTa                             <  2:1612/141‑1 (MQ=255)

                                                                                                                 cATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGtt                             >  2:67128/1‑140 (MQ=255)

                                                                                                                 cATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTa                             >  1:463381/1‑141 (MQ=255)

                                                                                                                   ttGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATg                           <  1:694201/141‑1 (MQ=255)

                                                                                                                    tGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGa                          >  2:654474/1‑141 (MQ=255)

                                                                                                                      ggTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaa                        >  2:866250/1‑141 (MQ=255)

                                                                                                                      ggTGTGCTTGTACAATTTGCCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaa                        >  2:345810/1‑141 (MQ=255)

                                                                                                                       gtgtGCTTTTACAATTTGTCTATTTTCGAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                       <  2:940931/141‑1 (MQ=255)

                                                                                                                       gtgtGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                       <  1:839486/141‑1 (MQ=255)

                                                                                                                       gtgtGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATACATCCTCTTGATCCAATTCATATAATAGTGTTGTTGAACAACGCTCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                       <  1:259227/141‑1 (MQ=255)

                                                                                                                            tttttACAATTTTTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataat                  <  1:410179/137‑1 (MQ=255)

                                                                                                                            cTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAACaataaa                  >  2:428429/1‑140 (MQ=255)

                                                                                                                            cTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataat                  <  1:1070638/141‑1 (MQ=255)

                                                                                                                            cTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataat                  <  1:240105/141‑1 (MQ=255)

                                                                                                                            cTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataaa                  >  2:938522/1‑140 (MQ=255)

                                                                                                                             ttGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAataata                 <  1:694010/141‑1 (MQ=255)

                                                                                                                              tGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATTAACAataataa                >  1:936956/1‑140 (MQ=255)

                                                                                                                                   aaTTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTAATTTCGGTGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACtt           <  1:524050/141‑1 (MQ=255)

                                                                                                                                   aaTTTGTCTATTTTCAAGATTGCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTTATTCTTGGTTTTTGTATGAAAAATAATACAACtt           <  1:59640/141‑1 (MQ=255)

                                                                                                                                       tGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGTAAAATAATACAACTTGCca       >  1:704162/1‑141 (MQ=255)

                                                                                                                                        gTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcat      >  1:513505/1‑141 (MQ=255)

                                                                                                                                        gTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcat      >  2:744406/1‑141 (MQ=255)

                                                                                                                                         tCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTGCTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcatc     >  1:135705/1‑141 (MQ=255)

                                                                                                                                         tCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcatc     >  1:979508/1‑141 (MQ=255)

                                                                                                                                         tCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcatc     >  1:666282/1‑141 (MQ=255)

                                                                                                                                         tCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcata     >  1:271976/1‑140 (MQ=255)

                                                                                                                                           tATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcatcat   >  1:262482/1‑141 (MQ=255)

                                                                                                                                            aTTTTCAAGATTTCTAATTACTTGCCCATCTTGACCCTATTCATTTAAATGTCTTTTTGCACCTATCGCGTTAATGACAACATTATATATGTCTACTGCTCCTTGGTTTTTGTACGACACATAACACAACGTACcatcata  >  2:193447/1‑140 (MQ=255)

                                                                                                                                            aTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTCGCCATCATg  >  1:230801/1‑141 (MQ=255)

                                                                                                                                            aTTTTCAAGATTGCTAATAAATTGACCATCTGGATCCAATTCATTTAAATGACTTTTTGCCCCTGTCGCGTTAATGACAACATTAGATATGTCTACTGATTCTTGGTTTTTGTATGAAAGATAATACATCTTGCCATCATg  <  1:571295/141‑1 (MQ=255)

TTTCGTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATCATG  >  USA300TCH1516_ALE/1899602‑1899882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`