BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,113,304	G→T	7.4%	H37N (CAT→AAT)	USA300HOU_RS10725 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,113,304	0	G	T	7.4%	693.7 / 7.5	236	H37N (CAT→AAT)	USA300HOU_RS10725	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (118/95); new base T (21/2); total (139/97)
Fisher's exact test for biased strand distribution p-value = 5.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 240 total aligned reads displayed.

AGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  USA300TCH1516_ALE/2113164‑2113443

aGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCATCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATg                                                                                                                                             <  1:783211/141‑1 (MQ=255)

   aTGTTAGTCACTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTc                                                                                                                                          <  2:869460/141‑1 (MQ=255)

      ttatctaTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGAt                                                                                                                                       <  2:379366/135‑1 (MQ=255)

      ttAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGAt                                                                                                                                       <  2:795008/141‑1 (MQ=255)

        aGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGt                                                                                                                                     >  1:966268/1‑141 (MQ=255)

         gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTCTTTTTCGATGTa                                                                                                                                    >  2:881674/1‑141 (MQ=255)

         gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                    >  2:635126/1‑141 (MQ=255)

         gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                    >  1:723595/1‑141 (MQ=255)

           cagtttCCTGTTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGACTAACTTTAAAACATGATACGGGGGCATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  >  2:499544/4‑141 (MQ=255)

           cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  <  2:492377/141‑1 (MQ=255)

           cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAAAGCTACAAAGACTTTTATATATTCCTACCATTGACACGAATACCTTAAAATCATGTAATTCTGTTATTA‑TTGTTTTTTCGTCATGGTCGGGGTaaa                                                                                                                                  >  1:808752/1‑141 (MQ=255)

            aTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATAATGGAATTCTGTAATTA‑TTGTTTTTTCGTCCTGTTCGATGTAAAt                                                                                                                                 >  2:288322/1‑141 (MQ=255)

                 ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTGCATAATTTTATCTTTTAAATCTTCTGACACGCATACCTTTAAATCATGGAATTCTGTATTTA‑TTGTTTTTTGGTCTTGGTTGATGTAAATTTCTa                                                                                                                            >  2:155239/1‑141 (MQ=255)

                 ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTa                                                                                                                            >  1:630376/1‑141 (MQ=255)

                  cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTGAATCTATAg                                                                                                                           >  1:135740/1‑141 (MQ=255)

                  cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           >  2:749347/1‑141 (MQ=255)

                     ttcaccctcATCAATTCCAGCCAATTCATACATAACTCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:740739/136‑1 (MQ=255)

                     ggcctcctCATCAAGTCCAGCCAATTCATACATACCACCGTCCATACTATGTTTTGTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:387360/139‑1 (MQ=255)

                      tcctccTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCTAGCGTa                                                                                                                       <  2:534079/141‑1 (MQ=255)

                      gcctcctCATCCATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTa                                                                                                                       <  1:192591/140‑1 (MQ=255)

                              atcaATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGc                                                                                                               >  1:435802/1‑141 (MQ=255)

                                caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                             <  1:1074977/141‑1 (MQ=255)

                                caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                             >  1:456933/1‑141 (MQ=255)

                                 aaTTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTaa                                                                                                            >  1:344442/1‑141 (MQ=255)

                                      cAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaa                                                                                                       >  1:327945/1‑141 (MQ=255)

                                      cAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaa                                                                                                       <  2:1095287/141‑1 (MQ=255)

                                       cgCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaa                                                                                                      <  1:118236/140‑1 (MQ=255)

                                        gCCAATTCATACATCACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaaa                                                                                                     <  1:906568/141‑1 (MQ=255)

                                        gCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTTGCGTACCATCAGCTAAATTaaaa                                                                                                     >  1:430957/1‑141 (MQ=255)

                                        gCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCGACTAAATTaaaa                                                                                                     >  1:653207/1‑141 (MQ=255)

                                             ttCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTATATTCAAAATAga                                                                                                >  2:865371/1‑140 (MQ=255)

                                                atacataACTCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGCTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCttt                                                                                             <  2:279884/141‑1 (MQ=255)

                                                  acataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTAt                                                                                           >  1:70807/1‑141 (MQ=255)

                                                           tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTCTCTTCActtt                                                                                  >  2:84076/1‑141 (MQ=255)

                                                           tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  1:876404/1‑141 (MQ=255)

                                                           tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  2:972846/1‑141 (MQ=255)

                                                           tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCGTTATCTTCTtttt                                                                                  >  1:297138/1‑141 (MQ=255)

                                                            ctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAACTCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTc                                                                                 <  2:130983/141‑1 (MQ=255)

                                                            ctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTCTGTAAATTTCTAGCGTACCATCAGTTAACTTAGAAATAGCTTTATCTCCGttttt                                                                                 >  2:781958/1‑140 (MQ=255)

                                                            ctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTCTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTCAATTTCTAGCGTACCATCAGCTAACTTAAACATAGCATTCTCTTCCTTTTc                                                                                 >  2:203019/1‑141 (MQ=255)

                                                              ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTGGCGTACCGTCAGCTAAATTAAAATTAGCTTTATTTTAATTTTCtt                                                                               >  2:509553/1‑141 (MQ=255)

                                                              ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtt                                                                               <  2:819638/141‑1 (MQ=255)

                                                               cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                              >  1:931966/1‑141 (MQ=255)

                                                                aTACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtttt                                                                             >  1:865033/1‑140 (MQ=255)

                                                                aTACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtttt                                                                             >  2:698115/1‑140 (MQ=255)

                                                                      ttATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTCTGTATCCt                                                                       >  1:560420/1‑141 (MQ=255)

                                                                         tCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATTTTCATTTTCTTTGTATCCtttt                                                                    >  2:706731/1‑141 (MQ=255)

                                                                            tttACATCTTCTGACACGAATACCTTTAAATCATGCAACTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCAGCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCt                                                                 <  1:94016/141‑1 (MQ=255)

                                                                             ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                                <  1:384795/141‑1 (MQ=255)

                                                                             ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                                >  1:200614/1‑141 (MQ=255)

                                                                             ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGGCCTGGTCGATGTAAATTTCTCGCGTACCATCAGCTACATTAAAAATAGCTTTATCTTCATTTTCTTTGTTTCCGTTTTCtt                                                                >  1:58306/1‑141 (MQ=255)

                                                                              tAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                               <  1:880320/141‑1 (MQ=255)

                                                                              tAAATCTTCTGACACGAATACCTTTAAATCAGGGAATTCT‑AATTCACCTACTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                               <  1:520905/141‑1 (MQ=255)

                                                                               aaaTCTTCTGACACGGATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTGTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:900801/141‑1 (MQ=255)

                                                                               aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTTTGTAAATTTCTAGCTTACCATCAGCTAAATTAGACTTAGCTTTTCCTTCGTTTTCTTTGTATCTtttttttttt                                                              >  2:613354/1‑141 (MQ=255)

                                                                               aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:48998/141‑1 (MQ=255)

                                                                               aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGAACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:664744/141‑1 (MQ=255)

                                                                                  tcttctGACACGGATACCTTTTCATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAAt                                                           <  2:302517/141‑1 (MQ=255)

                                                                                    ttctGACCCGAATAGCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCTTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAAttt                                                         <  2:191648/141‑1 (MQ=255)

                                                                                     tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAttttt                                                        >  2:554383/1‑140 (MQ=255)

                                                                                     tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCCTTTTCTTTGTATCCTTTTTTTTTTTAtttt                                                        >  2:939743/1‑140 (MQ=255)

                                                                                      ctGACACGAATACCTCTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATttct                                                       >  1:553765/1‑141 (MQ=255)

                                                                                         acacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTACTTTCTTCa                                                    >  1:83238/1‑141 (MQ=255)

                                                                                         acacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCa                                                    >  1:991390/1‑141 (MQ=255)

                                                                                          cacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAGTTAAAAATAGCTTTATCTTCATTTTCTTTGTTTCCTTTTTCTTTTATTTTGTTCaa                                                   >  2:107887/1‑141 (MQ=255)

                                                                                          cacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCaa                                                   >  2:943791/1‑141 (MQ=255)

                                                                                            cGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTAGCCTTTTTCTTTTAATTGCTTCaaaa                                                 >  1:499792/1‑140 (MQ=255)

                                                                                                  ccTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTTTTTTCTTCAATGATTcct                                           >  2:985673/1‑139 (MQ=255)

                                                                                                    tttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTa                                         >  2:1040480/1‑141 (MQ=255)

                                                                                                     ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAGTTAAAAATAGCTTTATGTTCGTGTTCTTTGTATCCTTTTTCTTTTATTTTATTCAGAGTTttattat                                        >  2:1013671/1‑140 (MQ=255)

                                                                                                     ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTaa                                        <  1:303081/141‑1 (MQ=255)

                                                                                                     ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAATACCGTTGTATCCTTTTTTTTTTAATTTTTTCAAAGATTCATTaa                                        >  2:433739/1‑141 (MQ=255)

                                                                                                      tAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAat                                       >  2:399721/1‑141 (MQ=255)

                                                                                                        aaTCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattt                                     <  2:456933/141‑1 (MQ=255)

                                                                                                         aTCATGCAATTCTGTAATTT‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAatttt                                    <  2:149945/141‑1 (MQ=255)

                                                                                                            aTGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAGGATTCATTAtttttcat                                 >  1:42127/1‑141 (MQ=255)

                                                                                                             tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  2:506039/1‑141 (MQ=255)

                                                                                                             tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  1:142988/1‑141 (MQ=255)

                                                                                                                 aTTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAATGATTTATTTatttttattttct                            >  2:677443/1‑140 (MQ=255)

                                                                                                                 aTTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTcc                            >  1:252198/1‑141 (MQ=255)

                                                                                                                 aTTCTGGAATTA‑TTGTTTTTTCTTCTTGTTCGAAGTAAATTTCTAGTGTACCATCAGCTCAATTAAAAATAGCTTTCTTTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCCTTATTTTTCATTTTcc                            <  2:898571/141‑1 (MQ=255)

                                                                                                                  ttCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCt                           <  1:609144/141‑1 (MQ=255)

                                                                                                                   tCTTTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                          <  1:323777/141‑1 (MQ=255)

                                                                                                                   tCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAGATAGCTTTATCTTCTTTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                          <  1:479517/141‑1 (MQ=255)

                                                                                                                    cTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTACATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGt                         <  2:597133/141‑1 (MQ=255)

                                                                                                                    cTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTAATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGt                         <  1:793615/141‑1 (MQ=255)

                                                                                                                       tAATTA‑TTGTTTTTTTTTTATTTTTTATGTAAATTACTAGCGTACCATCAGCTAAATTAGAATTAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTACTCAATGATTCATTACTTTTCATTTTCCTGTtcc                      >  2:618784/1‑141 (MQ=255)

                                                                                                                       tAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcc                      >  2:652386/1‑141 (MQ=255)

                                                                                                                         atta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCCTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcctc                    <  2:327945/141‑1 (MQ=255)

                                                                                                                         atta‑ctGTTGTCTCGTCATGGTCGATGTAAATTTCTAGCGTACCATAAAATAAATAAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcctc                    <  2:365996/141‑1 (MQ=255)

                                                                                                                           ta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct                  <  1:881674/141‑1 (MQ=255)

                                                                                                                           ta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct                  <  1:635126/141‑1 (MQ=255)

                                                                                                                                 ttttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCatat             <  1:1093875/141‑1 (MQ=255)

                                                                                                                                  tttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCGTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt            <  2:945380/141‑1 (MQ=255)

                                                                                                                                    tttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCa          <  1:396640/141‑1 (MQ=255)

                                                                                                                                        gTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGct      <  2:482974/141‑1 (MQ=255)

                                                                                                                                         tCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGctc     >  1:592790/1‑141 (MQ=255)

                                                                                                                                         tCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTCTCCCGTCCGTCCTCACATTCAAGctc     >  2:1056183/1‑141 (MQ=255)

                                                                                                                                          cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCATCATATTCAAActct    >  1:670581/1‑141 (MQ=255)

                                                                                                                                           aTGGTCGATGTCAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTACCTTCATTTTCTTTGTATCCTTTTACTTGTGGTTTCTTCTAAGATTCATTATTTTTCATTTTCCTGTTCCTCCTCATATTCAAGctctc   <  2:176858/141‑1 (MQ=255)

AGGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  USA300TCH1516_ALE/2113164‑2113443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`