BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,113,305	G→T	7.3%	D36E (GAC→GAA)	USA300HOU_RS10725 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,113,305	0	G	T	7.3%	702.4 / 6.1	236	D36E (GAC→GAA)	USA300HOU_RS10725	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (118/95); new base T (21/2); total (139/97)
Fisher's exact test for biased strand distribution p-value = 5.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 239 total aligned reads displayed.

GGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  USA300TCH1516_ALE/2113165‑2113443

ggATGTTGGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATgg                                                                                                                                            <  2:308161/141‑1 (MQ=255)

  aTGTTAGTCACTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTc                                                                                                                                          <  2:869460/141‑1 (MQ=255)

     ttatctaTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGAt                                                                                                                                       <  2:379366/135‑1 (MQ=255)

     ttAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGAt                                                                                                                                       <  2:795008/141‑1 (MQ=255)

        gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTCTTTTTCGATGTa                                                                                                                                    >  2:881674/1‑141 (MQ=255)

        gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                    >  2:635126/1‑141 (MQ=255)

        gTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTa                                                                                                                                    >  1:723595/1‑141 (MQ=255)

          cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  <  2:492377/141‑1 (MQ=255)

          cATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTaaa                                                                                                                                  >  2:1084129/1‑141 (MQ=255)

           aTTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATAATGGAATTCTGTAATTA‑TTGTTTTTTCGTCCTGTTCGATGTAAAt                                                                                                                                 >  2:288322/1‑141 (MQ=255)

                ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTGCATAATTTTATCTTTTAAATCTTCTGACACGCATACCTTTAAATCATGGAATTCTGTATTTA‑TTGTTTTTTGGTCTTGGTTGATGTAAATTTCTa                                                                                                                            >  2:155239/1‑141 (MQ=255)

                ccTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTa                                                                                                                            >  1:68963/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAAGGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATGCCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCtgg                                                                                                                           >  2:598823/1‑139 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTGAATCTATAg                                                                                                                           >  1:135740/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           <  1:623988/141‑1 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           >  2:749347/1‑141 (MQ=255)

                 cTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAg                                                                                                                           >  1:407224/1‑141 (MQ=255)

                    ttcaccctcATCAATTCCAGCCAATTCATACATAACTCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:740739/136‑1 (MQ=255)

                    ggcctcctCATCAAGTCCAGCCAATTCATACATACCACCGTCCATACTATGTTTTGTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGt                                                                                                                        <  2:387360/139‑1 (MQ=255)

                     tcctccTCATCTATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTa                                                                                                                       <  2:1024632/141‑1 (MQ=255)

                     tcctccTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCTAGCGTa                                                                                                                       <  2:534079/141‑1 (MQ=255)

                             atcaATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGc                                                                                                               >  1:435802/1‑141 (MQ=255)

                               caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                             >  1:456933/1‑141 (MQ=255)

                               caATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTa                                                                                                             <  1:1074977/141‑1 (MQ=255)

                                aaTTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTaa                                                                                                            >  1:344442/1‑141 (MQ=255)

                                     cAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaa                                                                                                       <  2:1095287/141‑1 (MQ=255)

                                     cAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaa                                                                                                       >  1:327945/1‑141 (MQ=255)

                                      cgCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTaaa                                                                                                      <  1:118236/140‑1 (MQ=255)

                                       gCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTTGCGTACCATCAGCTAAATTaaaa                                                                                                     >  1:430957/1‑141 (MQ=255)

                                            ttCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTACTTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTATATTCAAAATAga                                                                                                >  2:865371/1‑140 (MQ=255)

                                              catacataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCtt                                                                                              <  1:769320/141‑1 (MQ=255)

                                               atacataACTCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGCTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCttt                                                                                             <  2:279884/141‑1 (MQ=255)

                                               agacataACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTGCTAGCGTACGATCAGCTAAATTAGCAATAGCttt                                                                                             <  1:810118/139‑1 (MQ=255)

                                                     aaCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTc                                                                                       <  1:64580/141‑1 (MQ=255)

                                                      aCGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCa                                                                                      <  1:578953/141‑1 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTCTCTTCActtt                                                                                  >  2:84076/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  2:972846/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  1:872608/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAtttt                                                                                  >  1:876404/1‑141 (MQ=255)

                                                          tctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCGTTATCTTCTtttt                                                                                  >  1:297138/1‑141 (MQ=255)

                                                           ctcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTCTGTAAATTTCTAGCGTACCATCAGTTAACTTAGAAATAGCTTTATCTCCGttttt                                                                                 >  2:781958/1‑140 (MQ=255)

                                                            tcCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCt                                                                                >  1:19255/1‑141 (MQ=255)

                                                             ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTGGCGTACCGTCAGCTAAATTAAAATTAGCTTTATTTTAATTTTCtt                                                                               >  2:509553/1‑141 (MQ=255)

                                                             ccATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtt                                                                               <  2:819638/141‑1 (MQ=255)

                                                              cATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCttt                                                                              >  1:931966/1‑141 (MQ=255)

                                                               aTACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCtttt                                                                             >  2:698115/1‑140 (MQ=255)

                                                                tACTTTTATCTTTTAAATATTCTGAAACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTAGTCATGTTCGCTGTAAATTTATAGCGAACCATCAACTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGt                                                                            >  2:41683/1‑141 (MQ=255)

                                                                     ttATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTCTGTATCCt                                                                       >  1:560420/1‑141 (MQ=255)

                                                                        tCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATTTTCATTTTCTTTGTATCCtttt                                                                    >  2:706731/1‑141 (MQ=255)

                                                                           tttACATCTTCTGACACGAATACCTTTAAATCATGCAACTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCAGCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCt                                                                 <  1:94016/141‑1 (MQ=255)

                                                                            ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                                >  1:200614/1‑141 (MQ=255)

                                                                            ttAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtt                                                                <  1:384795/141‑1 (MQ=255)

                                                                             tAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                               <  1:880320/141‑1 (MQ=255)

                                                                             tAAATCTTCTGACACGAATACCTTTAAATCAGGGAATTCT‑AATTCACCTACTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCttt                                                               <  1:520905/141‑1 (MQ=255)

                                                                              aaaTCTTCTGACACGGATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTGTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:900801/141‑1 (MQ=255)

                                                                              aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTTTTGTAAATTTCTAGCTTACCATCAGCTAAATTAGACTTAGCTTTTCCTTCGTTTTCTTTGTATCTtttttttttt                                                              >  2:613354/1‑141 (MQ=255)

                                                                              aaaTCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGAACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCtttt                                                              <  1:664744/141‑1 (MQ=255)

                                                                                   ttctGACCCGAATAGCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCTTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAAttt                                                         <  2:191648/141‑1 (MQ=255)

                                                                                    tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTTTTTTTTTTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAttttt                                                        >  2:554383/1‑140 (MQ=255)

                                                                                    tctGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCTTTTTTTTTTATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCCTTTTCTTTGTATCCTTTTTTTTTTTAtttt                                                        >  2:939743/1‑140 (MQ=255)

                                                                                        acacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTACTTTCTTCa                                                    >  1:83238/1‑141 (MQ=255)

                                                                                        acacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCa                                                    >  1:991390/1‑141 (MQ=255)

                                                                                         cacGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCaa                                                   >  2:943791/1‑141 (MQ=255)

                                                                                           cGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAg                                                 >  1:970098/1‑141 (MQ=255)

                                                                                           cGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTAGCCTTTTTCTTTTAATTGCTTCaaaa                                                 >  1:499792/1‑140 (MQ=255)

                                                                                                aCCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTTTTCAAAGATTCa                                            >  2:13921/1‑141 (MQ=255)

                                                                                                aCCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTAATGGTCAATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCa                                            <  2:501440/141‑1 (MQ=255)

                                                                                                 ccTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTTTTTTCTTCAATGATTcct                                           >  2:985673/1‑139 (MQ=255)

                                                                                                 ccTTTAAATCAGGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAGATTTCTAGCGTACCATCAGCTGAATTAAAAGTAGCTTTATCATCATGTTCTTTGTGTCCTTTTTCTTTTAGTTTCTTCACAGATGCAt                                           >  2:24471/1‑141 (MQ=255)

                                                                                                   tttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTa                                         >  2:1040480/1‑141 (MQ=255)

                                                                                                    ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTaa                                        <  1:303081/141‑1 (MQ=255)

                                                                                                    ttAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCAATACCGTTGTATCCTTTTTTTTTTAATTTTTTCAAAGATTCATTaa                                        >  2:433739/1‑141 (MQ=255)

                                                                                                       aaTCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattt                                     <  2:456933/141‑1 (MQ=255)

                                                                                                        aTCATGCAATTCTGTAATTT‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAatttt                                    <  2:149945/141‑1 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTCATTTCTTCAAAGACTCAttctttttcttt                                >  1:321690/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  2:506039/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  1:142988/1‑141 (MQ=255)

                                                                                                            tGGAATTCTGTAATTA‑TTGATTTCTGGGGGGTGCCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCATTTTTCTTTTAATTTCTTCAAAGATTCATTAattttcatt                                >  1:534618/1‑141 (MQ=255)

                                                                                                                aTTCTGTAATTA‑TTGTTTTTTTTTTTTTTTTGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAATGATTTATTTatttttattttct                            >  2:677443/1‑140 (MQ=255)

                                                                                                                aTTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTcc                            >  1:252198/1‑141 (MQ=255)

                                                                                                                aTTCTGGAATTA‑TTGTTTTTTCTTCTTGTTCGAAGTAAATTTCTAGTGTACCATCAGCTCAATTAAAAATAGCTTTCTTTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCCTTATTTTTCATTTTcc                            <  2:898571/141‑1 (MQ=255)

                                                                                                                 ttCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCt                           <  1:609144/141‑1 (MQ=255)

                                                                                                                  tCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAGATAGCTTTATCTTCTTTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTg                          <  1:479517/141‑1 (MQ=255)

                                                                                                                   ttGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCCTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGt                         <  2:185155/140‑1 (MQ=255)

                                                                                                                      tAATTA‑TTGTTTTTTTTTTATTTTTTATGTAAATTACTAGCGTACCATCAGCTAAATTAGAATTAGCTTTATCTTCATTTTCTTTGTATCCTTTTTTTTTTAATTTACTCAATGATTCATTACTTTTCATTTTCCTGTtcc                      >  2:618784/1‑141 (MQ=255)

                                                                                                                      tAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcc                      >  2:652386/1‑141 (MQ=255)

                                                                                                                        atta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCCTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcctc                    <  2:327945/141‑1 (MQ=255)

                                                                                                                        atta‑ctGTTGTCTCGTCATGGTCGATGTAAATTTCTAGCGTACCATAAAATAAATAAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTtcctc                    <  2:365996/141‑1 (MQ=255)

                                                                                                                          ta‑ttGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTcctcct                  <  1:635126/141‑1 (MQ=255)

                                                                                                                                ttttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCatat             <  1:1093875/141‑1 (MQ=255)

                                                                                                                                 tttttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCGTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATAtt            <  2:945380/141‑1 (MQ=255)

                                                                                                                                   tttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCa          <  1:396640/141‑1 (MQ=255)

                                                                                                                                   tttCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCa          <  1:887134/141‑1 (MQ=255)

                                                                                                                                      cGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGc       >  1:487486/1‑141 (MQ=255)

                                                                                                                                       gTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGct      <  2:482974/141‑1 (MQ=255)

                                                                                                                                        tCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGctc     >  1:592790/1‑141 (MQ=255)

                                                                                                                                        tCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTCTCCCGTCCGTCCTCACATTCAAGctc     >  2:1056183/1‑141 (MQ=255)

                                                                                                                                         cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCATCATATTCAAActct    >  1:670581/1‑141 (MQ=255)

                                                                                                                                         cATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTATGGTGAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCATGctct    >  1:804565/1‑141 (MQ=255)

                                                                                                                                           tGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGctctct  >  1:923808/1‑141 (MQ=255)

GGATGTTAGTCATTTTCCTTGTCCTCCTCATCAATTCCAGCCAATTCATACATAACGCTCTCCATACTTTTATCTTTTAAATCTTCTGACACGAATACCTTTAAATCATGGAATTCTGTAATTA‑TTGTTTTTTCGTCATGGTCGATGTAAATTTCTAGCGTACCATCAGCTAAATTAAAAATAGCTTTATCTTCATTTTCTTTGTATCCTTTTTCTTTTAATTTCTTCAAAGATTCATTAATTTTCATTTTCCTGTTCCTCCTCATATTCAAGCTCTCT  >  USA300TCH1516_ALE/2113165‑2113443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`