Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,359,473 G→T 7.1% Q100K (CAA→AAA)  rpsM ← 30S ribosomal protein S13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,359,4730GT 7.1% 353.5 / 3.5 131Q100K (CAA→AAA) rpsM30S ribosomal protein S13
Reads supporting (aligned to +/- strand):  ref base G (74/45);  new base T (8/2);  total (83/48)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ATTCTTTTTCACTCTACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACGGATGCGACCTA  >  USA300TCH1516_ALE/2359337‑2359613
                                                                                                                                        |                                                                                                                                            
         ccctctACGTTTACGCGATACTTGTTGACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGGTTTAATTGGTCCTTTACGAGTACGCGCGTTGTTTTTCCTTTTTTGACCACGAACTGGt                                                                                                                                 <  1:672365/139‑1 (MQ=255)
         cACTCTACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGt                                                                                                                                 <  1:125299/141‑1 (MQ=255)
          aCTCTACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTGTTAACTGGTCCTTTTCGAGTTCGCGGGTGGTTACACGTTATCTAACCACAAAccgata                                                                                                                                >  1:78292/1‑136 (MQ=255)
          aCTCTACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTAATTATTATTTTTTCTTGTTAGATACCGTTTTAACTGTTACTTTACGACTACGCGCGTTGTTTTTAGTTTTTTGACCACGAAATgtga                                                                                                                                >  1:569332/1‑138 (MQ=255)
            tctACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTTGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTtaa                                                                                                                              >  2:1001976/1‑141 (MQ=255)
            tctACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTaaa                                                                                                                              >  1:786008/1‑141 (MQ=255)
             ctACGTTTACGAGATACTTGTTTACCTGCCATTTATTATTTGCCTTCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAAc                                                                                                                             <  1:1031064/141‑1 (MQ=255)
              tACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGACTCCTTTATCTTTTATGTTTTCTTGTTAGCTACCGTTTTAACATCTCTTCCACTATTACGCGCGTTGTTTTTCGTTTTTTTGCCACGAACTGGTAAAcc                                                                                                                            >  2:1062727/1‑141 (MQ=255)
                cGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAAATGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCac                                                                                                                          <  1:480296/141‑1 (MQ=255)
                  tttACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATAATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACCCGCGTCGTGTTTTGTTTTTTGACCACGCACTGGTAAACCacga                                                                                                                        >  1:419851/1‑141 (MQ=255)
                         gaTACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCCCGAACTGGTAAACCACGCCGGTGAc                                                                                                                 >  1:477885/1‑141 (MQ=255)
                         gaTACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCCCGAACTGGTAAACCACGACGGTGAc                                                                                                                 >  2:241083/1‑141 (MQ=255)
                         gaTACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTgtc                                                                                                                 >  1:967116/1‑139 (MQ=255)
                         gaTACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTgcg                                                                                                                 >  1:313755/1‑139 (MQ=255)
                         gaTACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTCTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGAc                                                                                                                 >  1:983669/1‑141 (MQ=255)
                         gaTAATTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGCATACTCGCGTTGTTTTTCGTTATTTGACCACGAACTGGTAAACCACGACGGTGAc                                                                                                                 >  1:752478/1‑141 (MQ=255)
                             cTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGAt                                                                                                             <  1:383857/141‑1 (MQ=255)
                                gTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTTACCACGATCTGGTAAACCACGACGGTGAAGGTTAcc                                                                                                          >  2:329752/1‑141 (MQ=255)
                                gTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATAcc                                                                                                          >  2:413283/1‑141 (MQ=255)
                                gTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATAcc                                                                                                          >  2:824428/1‑141 (MQ=255)
                                gTTTACGTGCCATTGAAAATTTGCGTCCATTACTTATTATTTTTTCTTGTTGGCTACCGTTTTAGGTGGTCCGTTAGGAGTACGCGAGTTGTTTTTCGTTTTTTGACCACGAACTGGTTGACCTCGACGGTGGAGGATAcc                                                                                                          >  2:836984/1‑141 (MQ=255)
                                      gTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTa                                                                                                    >  2:963090/1‑141 (MQ=255)
                                      gTGCCATTTAAAATTTGCCTCCTTTACCTATTATTGTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGtc                                                                                                    >  2:950212/1‑140 (MQ=255)
                                       tGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTat                                                                                                   >  2:561202/1‑141 (MQ=255)
                                          cATTTTAAATTTGCCTCCTTTACCTTTTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgat                                                                                                <  2:1031391/141‑1 (MQ=255)
                                           ttttGAACTTTGCCTCCTTTACCTGTTGTTTGTGCTTGTTAGCGCGCGGTTTAACTGGTCCTTTCCGAGTACGCGCGTTGTTGTTCGTTTTTTGTCCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgatg                                                                                               <  2:550841/140‑1 (MQ=255)
                                           aTTTACAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGACTCATACGATTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgatg                                                                                               <  1:255043/141‑1 (MQ=255)
                                           aTTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgatg                                                                                               >  1:139799/1‑141 (MQ=255)
                                            tttAAATTTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTAtgatga                                                                                              <  2:713369/141‑1 (MQ=255)
                                            tttAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTAtgatga                                                                                              <  2:509240/141‑1 (MQ=255)
                                             ttAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTTTCTGGTCCTTTACGAGTACGCGTGTTGTTTTTCGTTTTTTGATCTCGAACTGGTAATCTATGACGGTGGTGGATATCGCGGTttgttgga                                                                                             >  2:395402/1‑139 (MQ=255)
                                             ttAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGCTACCCACGTATGATTaa                                                                                             >  2:913063/1‑141 (MQ=255)
                                             ttAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACACCGGTATGATGaa                                                                                             >  2:406804/1‑141 (MQ=255)
                                              tAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGAGCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgat                                                                                                >  2:285091/1‑137 (MQ=255)
                                              tAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGTTAAACCCCGACGGTGACGGATACCACGGTATGATaaaa                                                                                            >  2:410339/1‑141 (MQ=255)
                                              tAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTatgat                                                                                                <  1:285091/137‑1 (MQ=255)
                                                 aaTTTGCCTCCTTTACCTATTCTTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAAttt                                                                                         <  2:498481/141‑1 (MQ=255)
                                                    ttgacccctTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCa                                                                                      <  2:495029/135‑1 (MQ=255)
                                                     tGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCAt                                                                                     >  1:834725/1‑141 (MQ=255)
                                                     tGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCAt                                                                                     <  2:952283/141‑1 (MQ=255)
                                                     tGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTCTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCAt                                                                                     >  2:244081/1‑141 (MQ=255)
                                                      gcttcctTACCCTATTATTCTTTCTTGGTAGCTACCGTTTTAACTAGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTAGACCACGAACTGGTACACCACGACGGTGACGGATACCACGGTATGATGAAATTGCCAtt                                                                                    <  2:378994/138‑1 (MQ=255)
                                                                ccTATTCTTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAACTTTCCATTAACCGTTTGa                                                                          >  2:676218/1‑141 (MQ=255)
                                                                ccTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGCATACCACGGTATGATGAAATTTCCATTAAACGTTTGa                                                                          >  2:943506/1‑141 (MQ=255)
                                                                  tattatTTTTTCTTGTTAGCTACCGTTTTAGCTGGTGCTTTACGAGTCCGCGCATTGTTTTTTGTTTTTTGACCACGAACTGGTAAACCACGCGCGCGACGGATGCCACGGTATGATGAAAATTCCATTAAACGTTTgtat                                                                        >  2:1005819/1‑138 (MQ=255)
                                                                  tattatTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGata                                                                        <  1:1057062/141‑1 (MQ=255)
                                                                    ttattGTTTCATGTGAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTACCATTAAACGTTTGATAtt                                                                      >  2:1098527/1‑141 (MQ=255)
                                                                        tttttCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTCCGGACGTTATTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGGATGATGAAATTTCCATTAAACGTTTGGTATTTAag                                                                  >  1:582813/1‑141 (MQ=255)
                                                                         ttttCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAagt                                                                 <  2:723335/141‑1 (MQ=255)
                                                                            tCTTGTTAGCTACCGTTTTACCTGGTCCTTTACGAGGACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACTACGGTGACGGATACCACGGTATGATGAAATTTCCATTTAACGTTTGATATTTAagttag                                                              <  1:233166/141‑1 (MQ=255)
                                                                            tCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACTACTGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAagttag                                                              <  1:423907/141‑1 (MQ=255)
                                                                             cTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAagttagt                                                             <  1:832325/141‑1 (MQ=255)
                                                                             cTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGCGACGGATACCACGGTATGATGAAATTTTCATTAAACGTTTTATCTTTAagttagt                                                             >  1:441072/1‑141 (MQ=255)
                                                                              ttGTTAGCTACAGTTTTAACTGGTCCTTTACGAGTACGCGCGTGGTTTTTCGTTTTTTGACCACGAACTGTTACACCACGACGCTGACTGATACCACGTTATGATGAAATTTCCCTTAAACGTTTGTTATTTAatttagtt                                                            >  2:263175/1‑141 (MQ=255)
                                                                                     cTACCGTTTTATCTGGTCCTTTACGAGTACGCGTGTTTTTTTTCTTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGACATTTAAGTTAGTTTCacgac                                                     <  2:613355/141‑1 (MQ=255)
                                                                                     cTACCGTTTTAACTGGTCCTTTACGAGGACGCGCGTTGTTTTTCGTTTTTTGACCACTAACTGGTAAACCACGACGGTGACGGATACCCCGGTATGATGAACTTTCCATTAAACGTTTGATATTTATGTTAGGTTCgcgac                                                     >  1:33912/1‑141 (MQ=255)
                                                                                       aCCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTCGTGTCACGACGt                                                   >  1:934331/1‑141 (MQ=255)
                                                                                         cGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTaa                                                 >  2:590918/1‑141 (MQ=255)
                                                                                            tttAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTc                                              >  2:532732/1‑141 (MQ=255)
                                                                                            tttAACTGGTCCTTTACGAGTACGCGCGCTCTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTc                                              <  1:851836/141‑1 (MQ=255)
                                                                                             ttAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTTTTTTTTTTTCCACGAACAGGTAAACCACGACGGTGACGGATACTACGGAAGGATGAAATTTCCGCCTAACGTTCCAAATTTAAGTTAGTTCCCCGACGTAAATcc                                             >  2:763641/1‑140 (MQ=255)
                                                                                             ttAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTGTGATGAAATTTCCATTAAACGTTTGATATTTAAGTTCGTTTCACGCCGTAAGTCa                                             >  1:1048255/1‑141 (MQ=255)
                                                                                             ttAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGTCGTAAGTCa                                             >  2:377877/1‑141 (MQ=255)
                                                                                              tAACTGGTCCTTTACGTGTACGCGCGTTGTTTTTCGTTTTTTGACCTCGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCAc                                            <  1:85616/141‑1 (MQ=255)
                                                                                                 cTGGTCCTTTACGAGTACGCGCGTTGTTTTTTTTTTTTTTTTCACGAAATGGTAACCCACGACGGTGACGGATGGTACGGGAGGGTGAAATTTTCATTTTACGTTTTATATTTTTTTTAGTTTCCCGTCGTAAGTCaaatt                                         >  2:1105756/1‑137 (MQ=255)
                                                                                                 cTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACTAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCtt                                         >  2:901467/1‑141 (MQ=255)
                                                                                                 cTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGCTGAAATTTCCATTAACCATTTGCTATTTAAGTTAGTTTCACGTCGTAATTcgcatt                                         >  1:472450/1‑136 (MQ=255)
                                                                                                 cTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACttt                                         >  2:106154/1‑141 (MQ=255)
                                                                                                          tACGAGTACGCGCGTTGTTTTTCGTTTTTTTTCTTTTTATTGATAAACCACCACGGTGACGGATAGCACGGTATGATGAAATTTCCATTAAACGTTTGATCTTTAAGTTAGTTCCACTACGTAAGTCACCTTCGACTTtat                                >  2:745974/1‑141 (MQ=255)
                                                                                                          tACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTtat                                <  2:139799/141‑1 (MQ=255)
                                                                                                            cGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGCTGAATTTTCCATTAATCGATTTATATTTAAGTTAGTTTCACGACGTAAGTCACCTTAGACTTTGTaa                              >  1:146145/1‑141 (MQ=255)
                                                                                                             gAGTACGCGCGTTGTTTTTCGTTTTTTTACCACGAACTGGTAAACCACGACGGTGACAGATACCACGGTATGATGAAATTTCCCTTCAACGTTTGATATTTCAGTTAGTTTCTCGCCATCAGTCACACTCAACTTTTTAAc                             >  2:583382/1‑141 (MQ=255)
                                                                                                             gAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACAACGACGGTGACGGATACTTCGGTACGATGAAATTTCCATTAAACGTTTGATATTTAAGTTTGTGTTACGACGTACGTCACCTTTGCCTGTATAAc                             >  2:1091423/1‑141 (MQ=255)
                                                                                                               gTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCg                           >  1:756720/1‑141 (MQ=255)
                                                                                                                 aCGCGCGTTGTTTTTTTTTTTTTTTCCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCTTc                         >  2:12170/1‑141 (MQ=255)
                                                                                                                   gcgcgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTa                       >  1:1002493/1‑141 (MQ=255)
                                                                                                                    cgcgTTGTTTTTCGTTTTTTGACCACGAAGTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTTTAc                      >  2:505898/1‑141 (MQ=255)
                                                                                                                    cgcgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTAc                      >  2:198397/1‑141 (MQ=255)
                                                                                                                    cgcgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACAGTGACGAATACCACGGTATGATGAAATTTACATTAAACATTTGATATTTAATTTAGTTTGACGACGTAAGTAACTTTCGACTTTCAAAACGTCTaa                      >  2:776157/1‑140 (MQ=255)
                                                                                                                     gcgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGCCTTTATAACCGTCTACa                     >  1:676595/1‑141 (MQ=255)
                                                                                                                     gcgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTCTGATCATTAAGATAATTTCACGAAGTAAGTCACCTTCGACTTTATAACCGTCTACa                     >  1:799721/1‑141 (MQ=255)
                                                                                                                      cgTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCTTCTACaa                    >  1:491525/1‑141 (MQ=255)
                                                                                                                       gttgttTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAAc                   <  1:963090/141‑1 (MQ=255)
                                                                                                                        ttgttTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATCACCGTCTACAACt                  >  1:365006/1‑141 (MQ=255)
                                                                                                                         tgttTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATTACCGTCTACAACtt                 <  2:750768/141‑1 (MQ=255)
                                                                                                                         tggttttCGTTTTTTTACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACtt                 <  2:16232/138‑1 (MQ=255)
                                                                                                                           tttttCTTTTTTTCACCACGACTCGGTAAACCACGACTGTTACGGATACCACGGTACGACGAAATTTCCATTAAACGTTTGCTATTTATGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  2:311948/141‑1 (MQ=255)
                                                                                                                           tttttCGTTTTTTGACCACTAACTGGTAAACCACGACGGTGAAGGAAACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  1:10804/141‑1 (MQ=255)
                                                                                                                           tttttCGTTTTTTGACCACGAACTGGTAAACCACGACGTTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  1:329752/141‑1 (MQ=255)
                                                                                                                           tttttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTACACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  1:1062727/141‑1 (MQ=255)
                                                                                                                           tttttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  1:824428/141‑1 (MQ=255)
                                                                                                                           tttttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCa               <  1:413283/141‑1 (MQ=255)
                                                                                                                             tttCGTTTTTTTGCCGCGTACTGGTAAACCACGACGGTTACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACg             <  2:96982/141‑1 (MQ=255)
                                                                                                                             tttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACg             <  2:796445/141‑1 (MQ=255)
                                                                                                                             tttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTCAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACg             <  2:488130/141‑1 (MQ=255)
                                                                                                                              ttCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACgg            >  1:352197/1‑141 (MQ=255)
                                                                                                                              ttCGTTTTTTGACCACGAACTGGTAAACAACGACGGTGACGGATACCACGGTAGGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACgg            >  2:671789/1‑141 (MQ=255)
                                                                                                                                  ttttttGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACGGATGc        <  1:911534/141‑1 (MQ=255)
                                                                                                                                   tttttGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTTCAACTTCACGTATGCg       >  1:75610/1‑141 (MQ=255)
                                                                                                                                      ttGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACGGATGCGAcc    <  1:1002687/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                            
ATTCTTTTTCACTCTACGTTTACGAGATACTTGTTTACGTGCCATTTAAAATTTGCCTCCTTTACCTATTATTTTTTCTTGTTAGCTACCGTTTTAACTGGTCCTTTACGAGTACGCGCGTTGTTTTTCGTTTTTTGACCACGAACTGGTAAACCACGACGGTGACGGATACCACGGTATGATGAAATTTCCATTAAACGTTTGATATTTAAGTTAGTTTCACGACGTAAGTCACCTTCGACTTTATAACCGTCTACAACTTCACGGATGCGACCTA  >  USA300TCH1516_ALE/2359337‑2359613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: