BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,560	T→C	10.0%	R210R (CGT→CGC)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,560	0	T	C	10.0%	297.2 / 17.0	109	R210R (CGT→CGC)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (65/32); new base C (6/5); total (72/37)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111421‑111699

 tatgTTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGTATTATAAAGATGCAGTTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                           <  2:1006019/138‑1 (MQ=21)

  aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                          <  2:355906/141‑1 (MQ=255)

  aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGACATATATCATATACTCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                          <  2:234489/141‑1 (MQ=17)

    gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAg                                                                                                                                        >  1:47529/1‑141 (MQ=255)

       ttttGCGAAATATAGGAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGata                                                                                                                                     <  2:541077/141‑1 (MQ=17)

          tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                                                                                  <  2:1033141/141‑1 (MQ=255)

           gcgcAATACAGCAGGTATAAAGATTTAATAAAGGGTTGAGATGGCGATCTATGTTTTATGCCTAAGGTGCCGAGGTATTCAGCTCAATATCAATTAACTAATGATGATTATAATTTAAAACAATTACGTAAAAGatataca                                                                                                                                 >  2:1063670/1‑138 (MQ=9)

            cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                >  1:1020955/1‑139 (MQ=255)

            cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                >  1:422354/1‑139 (MQ=255)

            cgcAATATAGCAATTTAAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                >  1:566018/1‑139 (MQ=14)

              cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGACTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              <  1:575036/141‑1 (MQ=255)

              aaaTATAGAAACTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              <  2:204009/140‑1 (MQ=255)

                atatAGCAATTTTAAATATTTAATGAATTATAAAGAAGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                                                                                                            <  1:463637/141‑1 (MQ=11)

                    aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACACTTACGCAAAAGATATGGTATAccggcg                                                                                                                        >  1:628213/1‑137 (MQ=14)

                     gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                       >  1:558718/1‑141 (MQ=255)

                         ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                                                                   <  1:436074/141‑1 (MQ=255)

                           ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAg                                                                                                                 <  1:849578/141‑1 (MQ=255)

                            tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAATAAATTACAGTACAAGACAAACCAATAATTAAAgc                                                                                                                >  2:826481/1‑141 (MQ=17)

                             aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               <  2:898253/141‑1 (MQ=255)

                             aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:941209/1‑141 (MQ=255)

                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACCATTAATTAAAAGATATGAACTAACAACAAATaaaccac                                                                                                              >  2:500281/1‑137 (MQ=17)

                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACACATAAAgggc                                                                                                              >  2:628194/1‑141 (MQ=255)

                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                                                              >  2:218881/1‑141 (MQ=255)

                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                                                              >  1:1009564/1‑141 (MQ=255)

                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTAGTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                                                              >  1:675116/1‑141 (MQ=255)

                                gATTTAATGAATTATAAATATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                                                                            <  2:817660/141‑1 (MQ=255)

                                 aTTTAATGAATTATAAAGATGAAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                                                           >  1:565898/1‑141 (MQ=255)

                                   ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                                                                         >  1:580233/1‑141 (MQ=255)

                                   ttAAAGAATTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                                                                         <  2:1054298/141‑1 (MQ=255)

                                               aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                                                             <  2:23284/141‑1 (MQ=255)

                                                  gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                          <  2:123680/141‑1 (MQ=255)

                                                   aTGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTa                                                                                         >  1:1099965/1‑141 (MQ=255)

                                                     ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACa                                                                                       <  1:36956/141‑1 (MQ=255)

                                                        gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  2:624094/141‑1 (MQ=255)

                                                        gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGACATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:731602/141‑1 (MQ=255)

                                                        gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAGTAACGAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:305466/141‑1 (MQ=255)

                                                              tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAATCGCCGAAGCTGTTGTTAAAAGGTACAGTGatgtta                                                                              >  2:1031032/1‑136 (MQ=255)

                                                               tatctaATTCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACCAGTACGTTAAAGAGATGACATACCAAAAAATAAACCGTCGAAGCTTTTGTTGAAAGTTACAGTGAATTTaa                                                                             <  2:213590/137‑1 (MQ=14)

                                                               cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                                                             <  2:325058/141‑1 (MQ=255)

                                                                    aaTCCAGAGGTGCCGAGGTATTCAGCTCAATATCAATTAACTAATGATGATTGTAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTCCAGGGGATTTAAAAGGt                                                                        >  1:995094/1‑141 (MQ=255)

                                                                      tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                      <  2:635608/141‑1 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGACGCTGTTGTTGAAAGGTAAAGGGAATTTAAAAGGTtca                                                                     >  2:840240/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTACAGGGTtca                                                                     >  1:452651/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  2:1032868/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:545035/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  2:531609/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTAAAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTGAAAAGGTcca                                                                     >  2:1082971/1‑141 (MQ=255)

                                                                         agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGGATTTAAAAGGTtcatc                                                                   >  1:763005/1‑141 (MQ=255)

                                                                           agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGGTTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                                                                 >  2:141727/1‑141 (MQ=255)

                                                                           agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                                                                 >  2:1101785/1‑141 (MQ=255)

                                                                            ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAGTTTAAAAGGTTCATCAGt                                                                >  2:464574/1‑141 (MQ=255)

                                                                             gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATCCAATGACATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:803427/1‑141 (MQ=17)

                                                                              tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  1:840809/1‑141 (MQ=255)

                                                                              tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACATAAAAGATATGACATACCAACAAATAAACCGCCGAAGCTGTTGTTGAAAAGTACGGGATAAGAAAAAGGTTCATAAGtgg                                                              >  1:197671/1‑139 (MQ=17)

                                                                               gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAGGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                                                             >  2:942874/1‑141 (MQ=255)

                                                                                ccGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa                                                            >  2:927725/1‑141 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                           >  2:695222/1‑141 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                           >  2:313699/1‑141 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                           >  1:481403/1‑141 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGaa                                                           >  2:629388/1‑140 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCAGCAGTTGGat                                                           >  2:961294/1‑141 (MQ=255)

                                                                                 cGAGTTATCCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgggt                                                           >  2:788898/1‑139 (MQ=255)

                                                                                   aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat                                                         <  2:622902/141‑1 (MQ=255)

                                                                                   aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGGTCATCAGTTGGataa                                                         >  2:632068/1‑140 (MQ=255)

                                                                                      tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa                                                      >  2:672780/1‑141 (MQ=255)

                                                                                      tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa                                                      <  2:373826/141‑1 (MQ=255)

                                                                                      tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATAACGGAAAAGAGATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa                                                      >  2:124684/1‑141 (MQ=255)

                                                                                        ttCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa                                                    >  1:1061013/1‑141 (MQ=255)

                                                                                        ttCAGCTCAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGATGATGAACGGTACGGGGAATTTAAAAGGTTCATCAGTTGGATATaacaa                                                    >  1:230094/1‑141 (MQ=14)

                                                                                           aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 >  1:871215/1‑141 (MQ=255)

                                                                                           aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 <  1:354424/141‑1 (MQ=255)

                                                                                           aGCTCAAAATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 >  1:696014/1‑141 (MQ=255)

                                                                                                  tatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAttt                                          <  1:527887/141‑1 (MQ=255)

                                                                                                  tatCAATTAACTAATGATGATTATAATGTAAAAAAATTACGTAAAAGATATGACATACCAACAACTAAAGCGCCGAAGCTGTTGTTGAAAGGCACAGGGCATTTAAAAGGTTCATCAGTTGGGTCTATACAAATTCAAttt                                          >  1:331877/1‑141 (MQ=14)

                                                                                                    tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                        >  2:352972/1‑141 (MQ=255)

                                                                                                    tCAATTAACTAATGATCATTACAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                        >  1:566787/1‑141 (MQ=255)

                                                                                                     cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACt                                       >  1:256592/1‑141 (MQ=255)

                                                                                                     cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACt                                       <  2:876055/141‑1 (MQ=255)

                                                                                                            cTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTag                                <  1:287482/141‑1 (MQ=255)

                                                                                                               atgatgATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAgaga                             >  2:580679/1‑141 (MQ=255)

                                                                                                               atgatgATTATAATGAAAAACAATTGCGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAgaga                             >  1:553718/1‑141 (MQ=255)

                                                                                                                 gatgatTATAATGTAAAATAATTACGTAAAAGATATGACATACCAACAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGTAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAAt                           <  2:131583/141‑1 (MQ=255)

                                                                                                                   tgatTATAATGTAAAACAATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                         >  1:669019/1‑141 (MQ=255)

                                                                                                                         taATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTTGATAATAAAAATGa                   >  2:990547/1‑141 (MQ=255)

                                                                                                                         taATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                   >  2:302650/1‑141 (MQ=255)

                                                                                                                         taATGTAAAACAATTACGCAAACAATATGACATACCAACAAATAAAGCGCCGACGCTGTTGTTGAAAGGCACAGGGAATTTAAAAGGTTAATAAGTAGGATATCAAAAAAATGAATTTACTTTTTTAGAGAATAAAAATGa                   >  2:305692/1‑141 (MQ=18)

                                                                                                                           aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:425081/1‑141 (MQ=255)

                                                                                                                           aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:50247/1‑141 (MQ=255)

                                                                                                                           aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:773234/1‑141 (MQ=255)

                                                                                                                                  aCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta          <  1:411904/141‑1 (MQ=255)

                                                                                                                                   cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         >  1:347442/1‑141 (MQ=255)

                                                                                                                                   cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         >  2:932174/1‑141 (MQ=255)

                                                                                                                                    aaTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttact        <  2:831021/141‑1 (MQ=255)

                                                                                                                                     aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTGGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttaatt       >  2:188131/1‑141 (MQ=255)

                                                                                                                                      ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt      >  1:370544/1‑141 (MQ=255)

                                                                                                                                      ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt      >  1:445941/1‑141 (MQ=255)

                                                                                                                                       taagTAAAAGATATGACATACCAACAGATAAAGCGGCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTCGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATATAAATGAAAATAtttacttta     <  1:668211/138‑1 (MQ=21)

                                                                                                                                       tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  1:951271/1‑141 (MQ=255)

                                                                                                                                         cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact   >  2:594976/1‑141 (MQ=255)

                                                                                                                                          gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  1:503983/1‑141 (MQ=255)

TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111421‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`