BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 954748	128 (1.150)	3 (0.040) +TTGGCATTTACGAAGTTTAAATGTGCAATTAGAATATATGTATAACAATATT	3/172	13.1	4.0%	intergenic (+402/+101)	gluD/glpQ_1	NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase
*	?	USA300TCH1516_ALE	2830190 =	104 (0.940)		3/172	13.1	4.0%	intergenic (+18/+429)	icaC/lipA_2	putative poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein/Lipase 1
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 132 total aligned reads displayed.

AGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGAAATTTGT  >  USA300TCH1516_ALE/954608‑954887

aGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCa                                                                                                                                             <  1:852026/141‑1 (MQ=255)

aGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCa                                                                                                                                             >  1:153267/1‑141 (MQ=255)

aGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCa                                                                                                                                             >  2:853518/1‑141 (MQ=255)

aGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCa                                                                                                                                             >  1:586604/1‑141 (MQ=255)

 gCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCaa                                                                                                                                            >  1:223959/1‑141 (MQ=255)

 gCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGGGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACCCCCaa                                                                                                                                            >  2:819083/1‑141 (MQ=255)

 aggagctaaTTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCaa                                                                                                                                            >  2:798218/8‑141 (MQ=255)

  ctcctcAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCaaa                                                                                                                                           >  1:742108/1‑141 (MQ=255)

      tcAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGa                                                                                                                                       >  4:386829/1‑141 (MQ=255)

              tACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTa                                                                                                                               <  3:291991/141‑1 (MQ=255)

              tACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTa                                                                                                                               >  1:581517/1‑141 (MQ=255)

              tACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTa                                                                                                                               >  1:862573/1‑141 (MQ=255)

               aCAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAg                                                                                                                              >  1:866800/1‑141 (MQ=255)

               aCAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAg                                                                                                                              >  3:225776/1‑141 (MQ=255)

               aCAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAg                                                                                                                              >  1:733987/1‑141 (MQ=255)

                   aCAAGGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATa                                                                                                                          <  2:504764/141‑1 (MQ=255)

                    ctaTGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATaa                                                                                                                         <  1:511163/139‑1 (MQ=255)

                     aaggCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAAt                                                                                                                        <  2:247617/138‑1 (MQ=255)

                         cAAGTTGGCGGGGCCCCAACACAGAGGCTGGCGGAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCa                                                                                                                    >  2:142876/1‑141 (MQ=255)

                           aGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAt                                                                                                                  >  2:320304/1‑141 (MQ=255)

                             ttGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAttt                                                                                                                <  1:491798/141‑1 (MQ=255)

                                gCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGa                                                                                                             <  1:291679/141‑1 (MQ=255)

                                gCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGa                                                                                                             >  2:476486/1‑141 (MQ=255)

                                   gggCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCCAAAAGACAGCAGTAGGATAATTTCCATTTGGAAat                                                                                                          >  1:183692/1‑141 (MQ=255)

                                   gggCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAat                                                                                                          >  1:616419/1‑141 (MQ=255)

                                     gCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAatat                                                                                                        <  1:824627/141‑1 (MQ=255)

                                        acAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTCCTGTCCCACTCCCAAAAAGCCCGCAGTAGGATAATTTCAATTTGGCAATATCtt                                                                                                     >  2:910170/2‑141 (MQ=255)

                                          aaCACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc                                                                                                   <  3:325647/141‑1 (MQ=255)

                                          aaCACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc                                                                                                   <  2:733987/141‑1 (MQ=255)

                                          aaCACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc                                                                                                   <  4:117916/141‑1 (MQ=255)

                                           acacAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAct                                                                                                  >  4:44910/1‑141 (MQ=255)

                                            cacaGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActg                                                                                                 >  1:381705/1‑141 (MQ=255)

                                             acaGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgc                                                                                                <  1:569279/141‑1 (MQ=255)

                                              caGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgct                                                                                               >  4:24854/1‑141 (MQ=255)

                                               aGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgctg                                                                                              <  1:782837/141‑1 (MQ=255)

                                                gAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt                                                                                             <  3:24854/141‑1 (MQ=255)

                                                 aaGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtt                                                                                            >  1:755269/1‑141 (MQ=255)

                                                 aaGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtt                                                                                            >  2:244874/1‑141 (MQ=255)

                                                  aGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttt                                                                                           >  1:878094/1‑141 (MQ=255)

                                                  aGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttt                                                                                           >  3:286872/1‑141 (MQ=255)

                                                     tGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt                                                                                        <  1:331254/141‑1 (MQ=255)

                                                      ggCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta                                                                                       >  1:117016/1‑141 (MQ=255)

                                                      ggCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta                                                                                       >  1:646553/1‑141 (MQ=255)

                                                      ggCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta                                                                                       >  1:259217/1‑141 (MQ=255)

                                                         ttttcgccAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAg                                                                                    >  2:611599/8‑141 (MQ=255)

                                                          aaaaGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGt                                                                                   >  1:880559/1‑141 (MQ=255)

                                                            tcgccAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAgata                                                                                 >  1:77248/5‑138 (MQ=255)

                                                                cAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGc                                                                             <  1:142876/141‑1 (MQ=255)

                                                                cAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGc                                                                             <  2:337254/141‑1 (MQ=255)

                                                                   agtaaaaTAGTGTGAAAATTGGGGTGGGACGACGAAACAAAATTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCtat                                                                          <  2:681039/136‑1 (MQ=37)

                                                                       cAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttt                                                                      >  4:197710/1‑141 (MQ=255)

                                                                       cAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttt                                                                      >  2:536307/1‑141 (MQ=255)

                                                                        aaTAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAtttt                                                                     <  1:115012/141‑1 (MQ=255)

                                                                        aaTAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAtttt                                                                     <  1:754551/141‑1 (MQ=255)

                                                                         aTAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt                                                                    <  3:261777/141‑1 (MQ=255)

                                                                              gtgCAAGGTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt                                                               <  4:239839/141‑1 (MQ=255)

                                                                               tgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAttt                                                              >  2:823867/1‑141 (MQ=255)

                                                                               tgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAttt                                                              >  2:789083/1‑141 (MQ=255)

                                                                                 cAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTAt                                                            >  1:301872/1‑141 (MQ=255)

                                                                                   aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGt                                                          >  3:112214/1‑141 (MQ=255)

                                                                                   aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGt                                                          >  2:178324/1‑141 (MQ=255)

                                                                                    gTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGtt                                                         >  1:231869/1‑141 (MQ=255)

                                                                                       ggggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAGAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc                                                      <  2:402438/141‑1 (MQ=255)

                                                                                            gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGtt                                                 <  1:77918/141‑1 (MQ=255)

                                                                                             ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt                                                >  1:856355/1‑141 (MQ=255)

                                                                                             ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt                                                >  1:303358/1‑141 (MQ=255)

                                                                                             ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt                                                >  1:856412/1‑141 (MQ=255)

                                                                                                   caaaataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt                                          <  2:250011/139‑1 (MQ=255)

                                                                                                    gaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTANATTTTTGTATTTATGNTTTCTAGTTTAACATta                                         <  1:615384/141‑1 (MQ=255)

                                                                                                      aataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtact                                       >  4:137631/1‑141 (MQ=255)

                                                                                                           atTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa                                  <  2:404046/141‑1 (MQ=255)

                                                                                                           atTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa                                  <  3:135820/141‑1 (MQ=255)

                                                                                                           atTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa                                  >  1:277730/1‑141 (MQ=255)

                                                                                                             tttGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt                                <  1:613168/141‑1 (MQ=255)

                                                                                                               tGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGa                              >  1:796262/1‑141 (MQ=255)

                                                                                                                gCGAAAATATCATTTCTGTCCCACTCACAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGAc                             <  2:631424/141‑1 (MQ=255)

                                                                                                                 cGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGAct                            <  2:742108/141‑1 (MQ=255)

                                                                                                                     aaTATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtctt                        >  3:126258/1‑141 (MQ=255)

                                                                                                                      atatCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCttt                       <  1:217291/141‑1 (MQ=255)

                                                                                                                        atCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtat                     <  3:228093/141‑1 (MQ=255)

                                                                                                                         tCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtata                    <  2:737631/141‑1 (MQ=255)

                                                                                                                         tCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtata                    <  1:14070/141‑1 (MQ=255)

                                                                                                                            tttCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATAttt                 >  3:305938/1‑141 (MQ=255)

                                                                                                                            tttCTGTCCCACTCAAAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATAttt                 <  4:370470/141‑1 (MQ=255)

                                                                                                                                tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCa             <  2:277730/141‑1 (MQ=255)

                                                                                                                                tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCa             >  2:700118/1‑141 (MQ=255)

                                                                                                                                tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCa             <  1:416113/141‑1 (MQ=255)

                                                                                                                                tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCa             >  3:223393/1‑141 (MQ=255)

                                                                                                                                 gTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact                                    >  3:75567/1‑117 (MQ=255)

                                                                                                                                  tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGc           <  1:178324/141‑1 (MQ=255)

                                                                                                                                  tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGc           <  1:819303/141‑1 (MQ=255)

                                                                                                                                   cccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCg          >  4:254839/1‑141 (MQ=255)

                                                                                                                                    ccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTTTTTTTATTTGTTCTATTGTAACATTACTACTTAATGACTTCTTtttatttagcgtcga         >  4:194454/1‑134 (MQ=255)

                                                                                                                                    ccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGa         <  2:878094/141‑1 (MQ=255)

                                                                                                                                     cccTCCCAAAAAAACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGaa        <  1:409059/139‑1 (MQ=255)

                                                                                                                                     cACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGaa        <  1:739267/141‑1 (MQ=255)

                                                                                                                                      aCTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGaaa       <  1:640979/141‑1 (MQ=255)

                                                                                                                                        tCCCAAAAAGACAGCAGTAGTATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGAAAtt     <  2:153267/141‑1 (MQ=255)

                                                                                                                                        tCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGAAAtt     >  3:278527/1‑141 (MQ=255)

                                                                                                                                           cAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGGTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGAAATTTGt  >  2:240364/1‑141 (MQ=255)

AGCTCCTCAATTTCTACAGACAATGCAAGTTGGCGGGGCCCCAACACAGAAGCTGGCGAAAAGTCAGCTTACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAGCGAAATTTGT  >  USA300TCH1516_ALE/954608‑954887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.