New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	100 (0.900)	53 (0.660) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	14/200	8.0	43.4%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
	?	USA300TCH1516_ALE	1426517 =	91 (0.820)					intergenic (‑248/+724)	pstS/cvfB	Phosphate‑binding protein PstS/Conserved virulence factor B
Rejected: Coverage evenness skew score above cutoff.

ATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGT  >  USA300TCH1516_ALE/1426380‑1426656                                                                                                                                             |                                                                                                                                              aTTGTTAAGAAAATGTAAATATTAATTTTTGAAAAACTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAAAACAAAAAAAAAca                                                                                                                                            <  2:262692/141‑1 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAAcagcag                                                                                                                                        >  2:555798/1‑141 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GACTGGGACATAAATCCCTAAAAAAAcagcag                                                                                                                                        >  4:107148/1‑141 (MQ=255)     ttAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCAAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGTGACATAAATCCCTAAAAAAAcagcag                                                                                                                                        >  1:481265/1‑141 (MQ=255)        aGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTaa                                                                                                                                     <  1:806138/141‑1 (MQ=255)        aGAAAATGTAAAAATAAATATTTATAAATCTAAAAAAGATTGCTTTATTAGTAAAATTAAAATGCTTCCTGAAAAATCATAGAAATTTAAATATGAATAC‑GAAAGT‑‑GGCTGGGATAAAAAAAAAAAAAAAAACAGCAGTaa                                                                                                                                     <  2:845268/141‑1 (MQ=255)           aaaTGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAt                                                                                                                                  <  1:355155/141‑1 (MQ=255)              tGTAAATATTAATTTTTTTTTTTTTTTAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACATCAGTAAGATAAt                                                                                                                               >  2:276906/1‑141 (MQ=38)                 aaaTATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAAtttt                                                                                                                            >  1:743161/1‑141 (MQ=255)                  aaTATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTc                                                                                                                           >  2:336474/1‑141 (MQ=255)                      ttAATNTTTGTGAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AAtt                                                                                                                      <  3:129133/141‑1 (MQ=255)                       tAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTa                                                                                                                     <  1:282523/141‑1 (MQ=255)                        aaTTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTctaattg                                                                                                                     <  3:102962/141‑7 (MQ=255)                           ttttGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGaaa                                                                                                                 <  1:169438/141‑1 (MQ=255)                             ttGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAa‑tt                                                                                                             <  1:192665/141‑2 (MQ=38)                             ttGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAa‑tt                                                                                                             <  1:163054/141‑2 (MQ=38)                              tGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCGTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAa‑ta                                                                                                             <  1:240287/141‑1 (MQ=255)                              tGTTAATATAAAAAATATTGCTTCATTAGTAAAATTAAAATGATTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAa‑ta                                                                                                             <  2:548027/141‑1 (MQ=255)                                ttAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATc                                                                                                           <  1:889228/141‑1 (MQ=255)                                 tAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCt                                                                                                          <  4:225020/141‑1 (MQ=255)                                  aaTCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCtt                                                                                                         <  2:27117/141‑1 (MQ=255)                                  aaTCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCtt                                                                                                         >  2:45162/1‑141 (MQ=255)                                   aTCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATTATCGAAATTTAAATATGTACACAAAAAATGAGGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATc                                                                                                           >  1:143572/1‑141 (MQ=21)                                    tCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTAc                                                                                                       >  3:46687/1‑141 (MQ=255)                                     cTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTAct                                                                                                      <  1:794638/141‑1 (MQ=255)                                        aaaaaTATTGCTTCATTAGTAAAATTAAAATGATAACTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATATTTTCT‑AATT‑GAAAATTATCTTActgct                                                                                                   <  2:205418/141‑1 (MQ=25)                                        aaaaaTATTACTATAATAGAAAAATTAAGTTGTTTCTTCAGTTTTTATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgct                                                                                                   <  3:14075/141‑1 (MQ=17)                                          aaaTATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGt                                                                                                 <  1:301117/141‑1 (MQ=255)                                            atatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               <  2:378628/141‑1 (MQ=255)                                            atatTGCTTCATTAGTAAAATTAAAATGCTTCATGAAAAATCATCGAAATTTAACAATAAAAAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               <  2:725391/141‑1 (MQ=25)                                             tatTGCTTCATTAGTAAGATTATAATGCTTCCGGAAGGAGCATCGAAAATTAAATAGGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  1:184519/141‑1 (MQ=21)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  1:567967/141‑1 (MQ=255)                                             tatTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  1:41552/141‑1 (MQ=255)                                             tatTGCTTAATTAGTAAAATTAAAATGCTTCCTGAAAAATAATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGAGACAAAAAAAAAAAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtct                                                                                              <  4:273879/141‑1 (MQ=25)                                              atTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtctc                                                                                             >  1:397126/1‑141 (MQ=255)                                               ttGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTctct                                                                                            >  1:606948/1‑141 (MQ=255)                                               ttGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTctct                                                                                            >  2:4545/1‑141 (MQ=255)                                               ttGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTctct                                                                                            >  2:585309/1‑141 (MQ=255)                                                   ttCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTAttt                                                                                        >  1:38046/1‑141 (MQ=255)                                                         aGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  <  4:325741/141‑1 (MQ=255)                                                         aGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  <  1:293718/141‑1 (MQ=255)                                                          gTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  <  1:764596/140‑1 (MQ=255)                                                          gTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACaa                                                                                  >  2:764596/1‑140 (MQ=255)                                                          gTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAAt                                                                                 <  3:371589/141‑1 (MQ=255)                                                            aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               >  3:289265/1‑125 (MQ=255)                                                            aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTtc                                                                                               <  4:289265/125‑1 (MQ=255)                                                            aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATAc                                                                               >  3:266204/1‑141 (MQ=255)                                                            aaaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATAc                                                                               >  1:162679/1‑141 (MQ=255)                                                             aaattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACt                                                                              <  1:360941/141‑1 (MQ=255)                                                              aattaaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATAcga                                                                             <  4:158223/141‑3 (MQ=255)                                                               attaaaatGCTTCCTAAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                            <  2:636183/141‑1 (MQ=255)                                                                 taaaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGt                                                                          <  1:291140/141‑1 (MQ=255)                                                                   aaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTAt                                                                        >  1:355674/1‑141 (MQ=255)                                                                   aaatGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTAt                                                                        >  3:230033/1‑141 (MQ=255)                                                                     atGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTg                                                                      <  4:65255/141‑1 (MQ=255)                                                                       gCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGaa                                                                    >  2:113319/1‑141 (MQ=255)                                                                        cTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAAt                                                                   >  2:371300/1‑141 (MQ=255)                                                                        cTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTAGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAAt                                                                   <  2:720225/141‑1 (MQ=255)                                                                          tCCTGAAAAATCATCAAAATTTAAAAATGAATAC‑AAAAAT‑‑GGCTGAGAAAAAAAAAAAAAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATgg                                                                 <  2:214920/141‑1 (MQ=17)                                                                           ccTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGc                                                                >  2:48320/1‑141 (MQ=255)                                                                              gAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                             <  2:746752/141‑1 (MQ=255)                                                                                aacatcatcGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGc                                                           >  1:713005/4‑141 (MQ=255)                                                                                 aaaTCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCtt                                                                                                         <  2:906255/94‑1 (MQ=255)                                                                                 aaaTCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCtt                                                                                                         >  1:906255/1‑94 (MQ=255)                                                                                      atcGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGcc                                                     >  1:496332/1‑141 (MQ=255)                                                                                      atcGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGcc                                                     >  4:352255/1‑141 (MQ=255)                                                                                       tcGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGccc                                                    <  1:371300/141‑1 (MQ=255)                                                                                          aaaTTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTc                                                 <  4:82878/141‑1 (MQ=255)                                                                                           aaTTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCt                                                <  3:239788/141‑1 (MQ=255)                                                                                           aaTTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCt                                                <  1:817012/141‑1 (MQ=255)                                                                                            aTTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTg                                               <  3:34111/141‑1 (MQ=255)                                                                                                aaaTATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTcac                                           <  2:38046/141‑1 (MQ=255)                                                                                                       aaTAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGACTTCGCTTTGCCCGTCTGTCACATTACTg                                    <  3:75461/141‑1 (MQ=255)                                                                                                        aTAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGt                                   <  2:313060/141‑1 (MQ=255)                                                                                                         tAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTa                                  <  1:555798/141‑1 (MQ=255)                                                                                                                        tGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATaaa                      >  2:39600/1‑141 (MQ=255)                                                                                                                        tGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATaaa                      >  1:640487/1‑141 (MQ=255)                                                                                                                        tGGGACATAAATCCCTAAAAAAACAGAAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATTGCTTCGCTTTTCCCGTCTGTCACATTACTGTAAAATTCtataca                      >  2:74715/1‑139 (MQ=255)                                                                                                                         gggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAAt                     <  2:628929/141‑1 (MQ=255)                                                                                                                          ggACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCtatttata                    <  3:166816/141‑6 (MQ=255)                                                                                                                          ggACATAAATCCCGAAAAAAACAGCAGTAAGATAATTTTG‑TATTAGAAAA‑TATCTGACTGCTGTGCTCTGTTTATACAATACTGCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATGAATa                    <  2:796019/141‑1 (MQ=14)                                                                                                                           gACATAAATCCGTAAAAAAGCAGCAGTGGGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTGGTAGTGAATGGCTTCGCTTTGCCCGTCTGTCACGTTACTGTAAGATTCTATAAATAg                   <  2:315800/141‑1 (MQ=14)                                                                                                                           gACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAg                   <  2:743161/141‑1 (MQ=255)                                                                                                                                aaaTCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAAttt              >  4:170492/1‑141 (MQ=255)                                                                                                                                   tCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtg           >  1:382615/1‑141 (MQ=255)                                                                                                                                       tAAAAAAACAGCAGTAAGATAATTTTT‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTTGCTTTGTCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtgatga       >  1:59912/1‑141 (MQ=255)                                                                                                                                       tAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTtgatga       <  2:53311/141‑1 (MQ=40)                                                                                                                                       tAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTCTCACATTACTGTAAAATTCTATCCATAGAATTTTtgatgc       >  4:290482/1‑140 (MQ=255)                                                                                                                                         agagaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGCTGACg     >  3:73/5‑141 (MQ=1)                                                                                                                                          aaaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAAt                     <  4:21002/124‑1 (MQ=40)                                                                                                                                          aaaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAAt                     >  3:21002/1‑124 (MQ=40)                                                                                                                                            aaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGt  <  3:189431/141‑1 (MQ=39)                                                                                                                                             |                                                                                                                                              ATTGTTAAGAAAATGTAAATATTAATTTTTGTTAATCTAAAAAATATTGCTTCATTAGTAAAATTAAAATGCTTCCTGAAAAATCATCGAAATTTAAATATGAATAC‑AAAAAT‑‑GGCTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTTTGCCCGTCTGTCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGT  >  USA300TCH1516_ALE/1426380‑1426656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.