Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,512,413	A→G	6.0%	intergenic (‑25/‑279)	USA300HOU_RS07370 ← / → USA300HOU_RS07375	Bacterial dynamin‑like protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,512,413	0	A	G	6.0%	165.4 / 2.0	62	intergenic (‑25/‑279)	USA300HOU_RS07370/USA300HOU_RS07375	Bacterial dynamin‑like protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01

TGCAAATATACTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATCAATTTT  >  USA300TCH1516_ALE/1512273‑1512550                                                                                                                                             |                                                                                                                                           tgcAAATATACTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTca                                                                                                                                            <  1:66687/141‑1 (MQ=255)   cAAATATACTTTCTTATTTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAGAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTcaca                                                                                                                                          <  2:104240/141‑1 (MQ=255)    aaaTATACTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACAt                                                                                                                                         >  2:24858/1‑141 (MQ=255)    aaaTATACTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACAt                                                                                                                                         <  2:768835/141‑1 (MQ=255)           cTTTTTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtg                                                                                                                                  <  1:349627/141‑1 (MQ=255)           cTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtg                                                                                                                                  <  1:398536/141‑1 (MQ=255)           cTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtg                                                                                                                                  <  1:862045/141‑1 (MQ=255)            tttCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtgc                                                                                                                                 >  2:714985/1‑141 (MQ=255)             ttCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATATAAAtgct                                                                                                                                >  2:744637/1‑141 (MQ=255)             ttCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtgat                                                                                                                                >  1:76421/1‑139 (MQ=255)                 tAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAAtgcttgct                                                                                                                            >  2:635128/1‑141 (MQ=255)                  aaTTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATgcttgctt                                                                                                                           >  1:100984/1‑141 (MQ=255)                          ggTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAAc                                                                                                                   <  2:877116/141‑1 (MQ=255)                          ggTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAAc                                                                                                                   >  1:454403/1‑141 (MQ=255)                          ggTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAAc                                                                                                                   <  1:145209/141‑1 (MQ=255)                                ttGTATGTAAAGGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATAGAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTc                                                                                                             <  2:76732/141‑1 (MQ=255)                                  ttacgtaAAAGTGCCTCATTTCGCGCCTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTTATTGTCATTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCag                                                                                                           <  2:399636/137‑2 (MQ=255)                                   tatgtaAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGCATTCATa                                                                                                          >  2:314343/1‑141 (MQ=255)                                    atgtaAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATACTTGCTTGTTACAACGAATTCATAc                                                                                                         >  1:61551/1‑141 (MQ=255)                                              gCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATTTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCGCATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATg                                                                                               >  2:430540/1‑141 (MQ=255)                                              gCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATg                                                                                               >  2:93387/1‑141 (MQ=255)                                              gCTTCATTTCGCGACTTTTCAACTTCGTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCGCATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATg                                                                                               >  2:587034/1‑141 (MQ=255)                                               gttCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGt                                                                                              <  1:15984/140‑1 (MQ=255)                                               cttcttTTCGCGACTTTTCAACGTCTTTTTTGTATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGt                                                                                              <  2:646959/141‑1 (MQ=255)                                                        gcgACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTaaa                                                                                     <  2:361352/141‑1 (MQ=255)                                                         cgACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTaaaa                                                                                    >  2:544797/1‑140 (MQ=255)                                                         cgACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAAc                                                                                    <  1:373384/141‑1 (MQ=255)                                                               ttCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTCTTCTTTATGAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GAt                                                                             <  1:405414/141‑1 (MQ=255)                                                                      tcttTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTaaa                                                                      <  1:635128/141‑1 (MQ=255)                                                                      tcttTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTaaa                                                                      <  2:650022/141‑1 (MQ=255)                                                                      tcgtgtgttAATTTATTTAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCGTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTaaa                                                                      <  2:462715/134‑1 (MQ=255)                                                                      tccttttttAATTTATATAGATTGGGTTATGCTTCTTTATTAATCATTTTACTTTCCCCTTTGGTTCGTTCACATAGCAACGCCCGCTTGTTACAACGAATTCATACACTATCGATGTCCATTTAAACTTT‑GATCGTTaaa                                                                      <  2:45286/138‑1 (MQ=255)                                                                         ttttttAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATgg                                                                   <  1:118042/141‑1 (MQ=255)                                                                              tAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGc                                                              <  1:753204/141‑1 (MQ=255)                                                                                 tttATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCCCATAGAAATGCTTGCTTGTTTCAACGAATTCATACAATATCAATGTCCATTTACA‑ATACGATCGTTACATGGCTCGCCtt                                                           >  2:469336/1‑141 (MQ=255)                                                                                  ttATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTg                                                          <  2:454403/141‑1 (MQ=255)                                                                                     tataAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGtat                                                       >  2:7153/1‑141 (MQ=255)                                                                                         aaaGATCTATTTGTTCTTTATTAATCTTGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAACTGGCTCGCATTGTATTATg                                                   <  1:200344/141‑1 (MQ=255)                                                                                           aGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCGCATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGtt                                                 <  1:430540/141‑1 (MQ=255)                                                                                           aGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCGCATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGtt                                                 <  1:587034/141‑1 (MQ=255)                                                                                                 aaTTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAAtt                                           >  2:351862/1‑141 (MQ=255)                                                                                                     gTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATTGCTCTCATTTTATTATGTTTAAATTTTAt                                       >  1:754603/1‑141 (MQ=255)                                                                                                     gTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTAt                                       >  1:713046/1‑141 (MQ=255)                                                                                                     gTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTAt                                       >  1:628506/1‑141 (MQ=255)                                                                                                        cTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAACCATA‑GATCGTTAAATGGCTCGCCTTGTATTATATTTAAATTTTATCAt                                    >  1:580525/1‑141 (MQ=255)                                                                                                        cTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACAGAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTGATctt                                    >  2:831846/1‑139 (MQ=255)                                                                                                           tattaATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAgc                                 <  1:24858/141‑1 (MQ=255)                                                                                                              taATCATGTTACTTTCCCCTTTGGCTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAgctgc                              <  2:603535/141‑1 (MQ=255)                                                                                                               aaTCATGTTACTTTCCGCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAgctgct                             >  2:812202/1‑141 (MQ=255)                                                                                                                  cATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGa                          <  1:888782/141‑1 (MQ=255)                                                                                                                  cATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGa                          >  2:738064/1‑141 (MQ=255)                                                                                                                  cATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTAAAACGATTTCGTACAATATCGATGTCCATTTAAACATA‑GATCGTTAAAAGGCTCGCATAGTATTATGTTTAAATTTTATCATAGCTTCTTGa                          >  1:81005/1‑141 (MQ=255)                                                                                                                    tGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATa                        <  1:714985/141‑1 (MQ=255)                                                                                                                      ttACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATAATAGCTGCTTGATaaa                      >  1:303136/1‑141 (MQ=255)                                                                                                                             ccccTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACg               <  2:1895/141‑1 (MQ=255)                                                                                                                              cccTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGa              <  1:704085/141‑1 (MQ=255)                                                                                                                                    ggTTCGTTCATATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATAGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATc        <  1:656065/141‑1 (MQ=255)                                                                                                                                    ggTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTTATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGTTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATc        <  1:257830/141‑1 (MQ=255)                                                                                                                                    ggTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCTTAGCTGCTTGATAAAACATACGAATAATc        >  1:215384/1‑141 (MQ=255)                                                                                                                                    ggTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGACTAATc        >  1:264800/1‑141 (MQ=255)                                                                                                                                    ggTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATc        <  1:87094/141‑1 (MQ=255)                                                                                                                                    ggTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATc        <  1:861132/141‑1 (MQ=255)                                                                                                                                          ttcACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATACTGAAtttt  >  2:327149/1‑141 (MQ=255)                                                                                                                                          ttcACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATCAAtttt  >  1:799385/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                           TGCAAATATACTTTCTTAATTACTTGGTTAATTGTATGTAAAAGTGCTTCATTTCGCGACTTTTCAACTTCTTTTTTTAATTTATATAAAAGATCTAATTGTTCTTTATTAATCATGTTACTTTCCCCTTTGGTTCGTTCACATAGAAATGCTTGCTTGTTACAACGAATTCATACAATATCGATGTCCATTTAAACATA‑GATCGTTAAATGGCTCGCATTGTATTATGTTTAAATTTTATCATAGCTGCTTGATAAAACATACGAATAATCAATTTT  >  USA300TCH1516_ALE/1512273‑1512550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑