Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,150,242 C→G 6.3% H208D (CAT→GAT)  agrA → Accessory gene regulator A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,150,2420CG 6.3% 232.2 / 5.2 78H208D (CAT→GAT) agrAAccessory gene regulator A
Reads supporting (aligned to +/- strand):  ref base C (30/41);  new base G (3/2);  total (33/45)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

TTTTTGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTGTAAATC  >  USA300TCH1516_ALE/2150102‑2150379
                                                                                                                                            |                                                                                                                                         
tttttGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGcc                                                                                                                                           >  2:34327/1‑141 (MQ=255)
  tttGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCat                                                                                                                                         >  1:805564/1‑141 (MQ=255)
   ttGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAGCCGTCAAATTGACTTTTATAGTAATTTAAAAGAACTCATGCAATTAGATGATCGTTTCTTTAGATGTCCTCATAGCTTTGTCGTCAATCCCCata                                                                                                                                        >  2:510140/1‑141 (MQ=255)
   ttGAATCATCAACAAAATCTCACAGACTCATTGACCCTTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTACATGATCGTTTCTTTAGATATCATAATAAATTCGTGGTCAATCGCCata                                                                                                                                        >  2:234332/1‑141 (MQ=255)
    tGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGGGGTCATAATAGCTTTGTCGTCAATCGCCataa                                                                                                                                       <  2:54148/141‑1 (MQ=255)
      aaTCATCAACAAAATCTAACAGACTCATTGCCCATTTAGATAACCGTCAAATTTAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCCTAATAGCTTCGTCGTCAATCCCCataata                                                                                                                                     >  2:551618/1‑141 (MQ=255)
       agcatcaACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTAATAATATCTTTGTCGTCAATCGCCATAatat                                                                                                                                    <  2:668110/139‑1 (MQ=255)
         catcaATAAAATCTCACCGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGATTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTg                                                                                                                                  <  1:277171/141‑1 (MQ=255)
             aacaaATGCTCACATACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTACTTTCGACATCATAATAGCTTTGCCATCACTCGCCTTAACATTGAATc                                                                                                                              <  2:935167/141‑1 (MQ=255)
               caaAATCTCACAGACTCAGTGACCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCta                                                                                                                            <  2:937441/141‑1 (MQ=255)
               caaAATCTCACACACTCATTGCCCATCTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCta                                                                                                                            <  2:853451/141‑1 (MQ=255)
                aaaaTCCCACAGACTCATTGCCCATTTAGATAACGGTCAAATTGAATTTTATGGTAATGTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGTCATTATATTGAATCtat                                                                                                                           <  1:394314/141‑1 (MQ=255)
                   aTCTCACAGACTCATTGCCCATTTAGATAACCGTCGAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGa                                                                                                                        >  1:596737/1‑141 (MQ=255)
                         caGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGaa                                                                                                                  >  1:406948/1‑141 (MQ=255)
                            aCTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAaga                                                                                                               <  2:817454/141‑1 (MQ=255)
                             cTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAagag                                                                                                              <  2:117722/141‑1 (MQ=255)
                                aTTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTCGATGTAATAATAGCTTTGTCGTCACTCGCCAGAATATTGAATTTATAGATTCGAAAGAGCGa                                                                                                           >  1:4227/1‑141 (MQ=255)
                                         ttAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCtct                                                                                                  >  2:845741/1‑139 (MQ=255)
                                           aGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTAttt                                                                                                <  2:588513/141‑1 (MQ=255)
                                           aGATAAACGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTAttt                                                                                                <  2:588685/141‑1 (MQ=255)
                                            gATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTAtttt                                                                                               <  1:34327/141‑1 (MQ=255)
                                                 ccGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCGATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTaaaaa                                                                                          >  2:754904/1‑141 (MQ=255)
                                                 ccGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCGATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTaaaaa                                                                                          >  2:285870/1‑141 (MQ=255)
                                                     cAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATGTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATaaa                                                                                      >  1:798443/1‑141 (MQ=255)
                                                      aaaTTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCTTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGAGAATTGTCTATTTTAAAATTAAAg                                                                                     >  2:451007/1‑141 (MQ=255)
                                                      aaaTGGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAAGATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATaaaa                                                                                     >  2:571798/1‑140 (MQ=255)
                                                       aaTTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGa                                                                                    >  2:141859/1‑141 (MQ=255)
                                                           gAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTGTAGATTCAAAAGAGCTAATTGTCTATTTTAAAAATAAAGAacac                                                                                >  1:404814/1‑141 (MQ=255)
                                                            aaTTTTTTAGTAATTTAAAAGAACTGAGTCAATTAAATGATCGTTTCTTTAGATGTCATAATAGCTGTGTCGTCAATCGTCAGAATATTGAATCTATAGATTCGAAAGTGCGAATTGTCTATTTTAAAAATAAAGAACACt                                                                               <  2:104341/141‑1 (MQ=255)
                                                             aTTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACta                                                                              >  2:841215/1‑140 (MQ=255)
                                                             aTTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTg                                                                              >  1:149336/1‑141 (MQ=255)
                                                             aTTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTg                                                                              >  2:323290/1‑141 (MQ=255)
                                                              ttttATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGc                                                                             <  2:864097/141‑1 (MQ=255)
                                                                    ggTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCa                                                                       <  2:886301/141‑1 (MQ=255)
                                                                     gTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCAt                                                                      <  1:70758/141‑1 (MQ=255)
                                                                      tAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATc                                                                     <  2:200542/141‑1 (MQ=255)
                                                                       aaTTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTAAGCATCg                                                                    >  2:560906/1‑141 (MQ=255)
                                                                         tttAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGt                                                                  <  1:746172/141‑1 (MQ=255)
                                                                             aaaGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTgaga                                                              >  1:215174/1‑141 (MQ=255)
                                                                             aaaGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTgaga                                                              >  2:60153/1‑141 (MQ=255)
                                                                             aaaGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTgaga                                                              >  2:872283/1‑141 (MQ=255)
                                                                                 aattgcctCAATTAGGTGATCGTTGCTACAGTTGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACg                                                          <  1:442949/134‑1 (MQ=255)
                                                                                 aaCTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACg                                                          <  2:808243/141‑1 (MQ=255)
                                                                                  acggAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGt                                                         <  2:921444/138‑1 (MQ=255)
                                                                                   cTGATTCAATTAGATGATCGTTTCTTTAGATGACATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCAGCGGTGAGAAACGtt                                                        >  1:674884/1‑141 (MQ=255)
                                                                                   cTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGtt                                                        <  2:683107/141‑1 (MQ=255)
                                                                                   attaGTCAATTAGATGAGTGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGtt                                                        <  1:112751/138‑1 (MQ=255)
                                                                                     gAGTCAATTAGAGGATCTCTTCTTTATTTGTCATAATAGCTTTGTCGTCAATCGCAATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaa                                                      <  2:48102/141‑1 (MQ=255)
                                                                                      aGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaa                                                     >  1:798925/1‑141 (MQ=255)
                                                                                      aGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaa                                                     <  1:621577/141‑1 (MQ=255)
                                                                                      aGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaa                                                     >  1:505942/1‑141 (MQ=255)
                                                                                      aGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaa                                                     <  1:594499/141‑1 (MQ=255)
                                                                                      aGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaa                                                     >  2:115462/1‑141 (MQ=255)
                                                                                         cAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaaaaa                                                  <  2:406948/141‑1 (MQ=255)
                                                                                         cAATTAGATCATCGTTTCTGTATAGTTCACGATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaaaaa                                                  <  1:18280/141‑1 (MQ=255)
                                                                                          aaTTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTaaaaaaa                                                 <  1:146220/141‑1 (MQ=255)
                                                                                           aTTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGCTAAAAAAat                                                <  2:847029/141‑1 (MQ=255)
                                                                                                  gatTGTTTCTTTAGATGTCGTAATAGCTGTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATAtaataa                                         <  2:298140/141‑1 (MQ=255)
                                                                                                  gatCGTTTATTTATATGGCATAATAGCTGTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATAtaataa                                         <  2:608413/141‑1 (MQ=255)
                                                                                                    tCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGa                                       >  1:773079/1‑141 (MQ=255)
                                                                                                       tttCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGataa                                    <  1:854447/141‑1 (MQ=255)
                                                                                                       tttCTTAAAAAGTCAAAAAAGCTGTGTCGACAAACGCCATAATATTTAATCTATAGATTCGAAAGAGCGAATTGTCTAATTTAAAAATAAAGAACACTGCTATGCATCGGTGAAAAAAAAAAAAAAAATATAATAATataa                                    <  2:575835/141‑1 (MQ=255)
                                                                                                        ttCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATTTAATAAGataat                                   >  2:12945/1‑141 (MQ=255)
                                                                                                          cTTTAGATGTCATAATAGCTTTGACGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAAAAAAGATAAAAAAATATAATAAGAtaataa                                 <  2:929261/141‑1 (MQ=255)
                                                                                                                aTGTCATAATAGCTTTGTCGTCAATCGCGCTACTATTGAATCTATAGATTCGAACGAGCGCATTTTCTATTCTAAAAACAAGGGACACTGCTAGGCATCTATGACTAACGTTAAAAAAATATAATAAGATAATAAATTCAg                           >  2:316146/1‑141 (MQ=255)
                                                                                                                aTGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCaa                           >  2:352194/1‑140 (MQ=255)
                                                                                                                 tGTCATAATTGCTTCGTCGTCAATCGACATAATATTGAATCTATAGATTAGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGt                          <  1:610008/141‑1 (MQ=255)
                                                                                                                 tGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGt                          <  1:347855/141‑1 (MQ=255)
                                                                                                                   tCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTa                        <  1:634911/141‑1 (MQ=255)
                                                                                                                         taGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCg                  <  1:754079/141‑1 (MQ=255)
                                                                                                                         taGCTTTGTCGACAATCGTTATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTATATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCg                  <  2:508468/141‑1 (MQ=255)
                                                                                                                              ttGTCGTCAATCGCCATAATATTGAATCTATAGATTCGATAGAGCGAGTTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATGATAAAGTCAGTTAACGGCGTATTc             >  2:590265/1‑141 (MQ=255)
                                                                                                                                   gtcAATCGCGATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTg        <  1:285870/141‑1 (MQ=255)
                                                                                                                                   gtcAATCGCGATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTg        <  1:754904/141‑1 (MQ=255)
                                                                                                                                   gtcAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGGACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTGAGTTAACGGCGTATTCAAttt        >  2:499000/1‑140 (MQ=255)
                                                                                                                                    tcAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTGt       >  1:848007/1‑141 (MQ=255)
                                                                                                                                     cAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCAGCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTGTa      <  2:520704/141‑1 (MQ=255)
                                                                                                                                      aaTCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAATAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCTGTTAACGGCGTATTCAATTGTaa     <  2:674884/141‑1 (MQ=255)
                                                                                                                                         cGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTGTAAATc  <  1:398116/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTTTTGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATTGAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCATAATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTCTATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAATAAGATAATAAAGTCAGTTAACGGCGTATTCAATTGTAAATC  >  USA300TCH1516_ALE/2150102‑2150379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑