BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,560	T→C	10.0%	R210R (CGT→CGC)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,560	0	T	C	10.0%	297.8 / 21.3	114	R210R (CGT→CGC)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (56/44); new base C (2/9); total (59/55)
Fisher's exact test for biased strand distribution p-value = 2.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 114 total aligned reads displayed.

TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111422‑111699

tAAGTTTTTTGCGCAATATTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                           <  1:426726/141‑1 (MQ=255)

tAAGTTTTTTGAGCTTTATAGTGTTGTTGGAGATTTAGTGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGTGTTTTTTAGATAATTATCGATTAAGTAATGTTGATTTTAATGTAACACGTTTAAGTaa                                                                                                                                           >  2:523232/1‑141 (MQ=255)

 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                          <  1:656757/141‑1 (MQ=255)

     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                                      <  2:245610/141‑1 (MQ=255)

     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                                      <  1:364575/141‑1 (MQ=255)

      ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGata                                                                                                                                     <  1:39596/141‑1 (MQ=255)

           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                <  2:521534/141‑3 (MQ=255)

           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGCagaagagaagaaa                                                                                                                                >  1:265382/1‑134 (MQ=17)

            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGatat                                                                                                                               <  2:654569/141‑4 (MQ=14)

            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAGTGCGTAAAAGATATGACAt                                                                                                                               >  1:716860/1‑141 (MQ=255)

                tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                                                           <  2:799780/141‑1 (MQ=255)

                 ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                                                          <  2:136651/141‑3 (MQ=14)

                 ataGCAAGTTAAAAGATTTAATGAATTATAAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                                                          <  2:213889/141‑3 (MQ=11)

                  taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                                                                         <  2:734338/141‑1 (MQ=255)

                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacga                                                                                                                       >  1:859404/1‑136 (MQ=11)

                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGGCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                                                      <  2:222652/141‑7 (MQ=11)

                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATGATGTTATACAATTACGTAAAAGATATGACATACcaacaat                                                                                                                      >  1:405531/1‑140 (MQ=255)

                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAACAACaaa                                                                                                                      >  2:925609/1‑141 (MQ=255)

                         tttAAAGATTTAATGAATTACAAAGATGGAGATTTATCATATAATCCAGAGGTTCCGTGTTATTCAGCTCAATCTCAATTAACTAATGATTATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                  <  2:365894/141‑1 (MQ=17)

                         tttAAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAAGTATTCAGCTCAAAATCAATTAACAAATGATGATTGTAATGGAAAACAATTACGTCAAAGATGTGACATACAAACAaaaaaa                                                                                                                  >  2:226084/1‑141 (MQ=255)

                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  2:385343/1‑141 (MQ=255)

                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:256927/1‑141 (MQ=255)

                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:500599/1‑141 (MQ=255)

                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATCTCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAACTAAAGCGcc                                                                                                             >  2:323140/1‑141 (MQ=255)

                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                             <  1:896797/141‑1 (MQ=255)

                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                             <  1:15191/141‑1 (MQ=255)

                                 tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                          <  2:667357/141‑1 (MQ=255)

                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                      <  2:353805/141‑1 (MQ=255)

                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                     <  1:882051/141‑1 (MQ=255)

                                         aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                                                                  <  2:38893/141‑1 (MQ=255)

                                               aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                            >  2:349237/1‑141 (MQ=255)

                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                           >  2:587255/1‑141 (MQ=255)

                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:578903/141‑1 (MQ=255)

                                                       gaTATATCATATAATCACGGGGTGACGCATCTTTAAGCTCAATCACACTTAAATAATGACGATTACAAGGCAAAACAATTACAAAAAATAGATTACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  2:315593/141‑1 (MQ=255)

                                                       gaTATAGTATAGACTCNAGAGGTGCCGAGTTATTCAGCTCAATATGGATTATCTAATGAGGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:883703/141‑1 (MQ=17)

                                                       aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:385740/140‑1 (MQ=255)

                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                                                                                   >  1:606642/1‑141 (MQ=255)

                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                                                                                   >  1:607557/1‑141 (MQ=255)

                                                           tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                                >  2:797177/1‑141 (MQ=255)

                                                           tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                                >  1:404150/1‑141 (MQ=255)

                                                               atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTaaa                                                                            >  2:61924/1‑141 (MQ=255)

                                                                   aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                        <  1:449599/141‑1 (MQ=255)

                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATCTCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:588212/141‑1 (MQ=255)

                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:449899/141‑1 (MQ=255)

                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:784135/141‑1 (MQ=255)

                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGCTATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                                                                   >  2:568478/1‑141 (MQ=255)

                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGGGAATTTAAAAGGTtcatc                                                                   >  1:296759/1‑141 (MQ=255)

                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATCATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATACCAACAAATAAACAGCCGGAGCGATTGTTGAAAGGTACAGGGAATTTAAAAgatacaca                                                                   >  2:81327/1‑134 (MQ=17)

                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                                                                >  1:355534/1‑141 (MQ=255)

                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:630576/1‑141 (MQ=255)

                                                                             tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  1:697651/1‑141 (MQ=255)

                                                                             tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  2:907260/1‑141 (MQ=255)

                                                                             tGCCGAGTTATTCAGCACAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  1:524529/1‑141 (MQ=255)

                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAGAGGGAATTTAAAAGGTTCATCAGTTGGat                                                           >  1:171556/1‑141 (MQ=255)

                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGaa                                                           >  2:352391/1‑140 (MQ=255)

                                                                                   gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCAGAAGTTGGCtata                                                        >  1:398262/1‑141 (MQ=255)

                                                                                         cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                                  >  1:798485/1‑141 (MQ=255)

                                                                                          aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAATAAATAAAGCGCCGAAGAAGTTGTTGGAAGGTAGAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 >  2:704851/1‑141 (MQ=17)

                                                                                          aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 <  2:279448/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAGAGTCATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCATTTGGATATAAAAAAATTGAAt                                            <  2:648610/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:849179/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:535795/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  2:875176/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:136711/141‑1 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            >  1:663116/1‑141 (MQ=255)

                                                                                               aaTATCAATTAACTAATGATGATTATAATGAAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGTTACAGGGAATTTAAAAGGTTCATCAGAAAAATAAAAAAAAATTGAAt                                            <  2:608460/141‑1 (MQ=14)

                                                                                               aaTATAAATTAACTAATGATGAGTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  2:444493/141‑1 (MQ=255)

                                                                                                atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTAAAAGATTCATCAGTTGGATATAAAAAAATTGaaat                                           >  2:94576/1‑139 (MQ=255)

                                                                                                atagCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTGGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTGGGATATAAAAAAATTGAAtt                                           >  2:476273/1‑141 (MQ=255)

                                                                                                 tatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAttt                                          >  1:347194/1‑141 (MQ=255)

                                                                                                   tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                        <  1:81933/141‑1 (MQ=255)

                                                                                                    cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACt                                       >  1:895372/1‑141 (MQ=255)

                                                                                                      aTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGGTATAAAAAAATTGAATTTACttt                                     >  1:8204/1‑141 (MQ=255)

                                                                                                       ttAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCACCCAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttt                                    >  1:220616/1‑141 (MQ=255)

                                                                                                            tAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCCCGAAACAGAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAGGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTaga                               <  1:925609/141‑1 (MQ=17)

                                                                                                            aaataatGAATAAAAAGAAAAACAAATACGTAAAAGATATAACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAAGTACAGAGAATTTAAAAGGTTCATCAGTTGAATATAAAAAAATTGAATTTACTTTTTTaga                               <  2:405531/140‑1 (MQ=17)

                                                                                                             aaTGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                              <  1:587255/141‑1 (MQ=255)

                                                                                                               tgatgaTTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            >  1:942175/1‑141 (MQ=255)

                                                                                                               tgatgaTTATAATGTAAAAAAATTACGAAGAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            >  1:935743/1‑141 (MQ=255)

                                                                                                               taatgatTATAATGTAAAACAATTACGTAAAATATATGACATACCAACAAATAAAGCGGCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:736950/139‑1 (MQ=255)

                                                                                                               aaatgatTATAATGTAAAACAATTACGTAAAAAATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTAAAATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:268209/139‑1 (MQ=255)

                                                                                                                  tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                         >  2:672137/1‑141 (MQ=255)

                                                                                                                   aatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATAGAATTAACTTTTTTAGAGAATaaa                        <  1:803099/140‑1 (MQ=255)

                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  >  1:304094/1‑141 (MQ=255)

                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  <  2:221281/141‑1 (MQ=255)

                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  >  2:398752/1‑141 (MQ=255)

                                                                                                                          aTGTAAAACCATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:692197/1‑141 (MQ=255)

                                                                                                                          aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGTATAAAAATGaaa                 >  2:297533/1‑141 (MQ=255)

                                                                                                                             tAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata              <  1:349237/141‑1 (MQ=255)

                                                                                                                                aaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttt           >  2:556835/1‑141 (MQ=255)

                                                                                                                                 aCAATTACGTAAAAGATATGACATACCAACAAATAAATCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta          <  1:827900/141‑1 (MQ=255)

                                                                                                                                 aCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta          >  1:928835/1‑141 (MQ=255)

                                                                                                                                  cgaTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAACCACTTGCGTTGACTTTTTTCGAGAATAAAAATGTAAATAtttac         <  1:690544/139‑1 (MQ=18)

                                                                                                                                  cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         >  1:396527/1‑141 (MQ=255)

                                                                                                                                    aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactt       >  1:219575/1‑141 (MQ=255)

                                                                                                                                      tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  2:12508/1‑141 (MQ=255)

                                                                                                                                      tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  2:487889/1‑141 (MQ=255)

                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGATTTTACTTTTTTAGAGAATAAAAATGAAAATGTTTACTTTACTg  >  1:277175/1‑141 (MQ=255)

                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  2:278744/1‑141 (MQ=255)

                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  2:278752/1‑141 (MQ=255)

TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  USA300TCH1516_ALE/111422‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`