Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,899,742	A→G	N421N (AAT→AAC)	USA300HOU_RS09405 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,899,742	0	A	G	100.0%	329.7 / NA	90	N421N (AAT→AAC)	USA300HOU_RS09405	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/56);  total (34/56)

GTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATC  >  USA300TCH1516_ALE/1899606‑1899879                                                                                                                                         |                                                                                                                                           gTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGtttt                                                                                                                                        <  2:492877/141‑1 (MQ=255)  tAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTc                                                                                                                                       <  3:234548/141‑1 (MQ=255)  tAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTc                                                                                                                                       >  2:365440/1‑141 (MQ=255)    gTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCaa                                                                                                                                     <  3:158115/141‑1 (MQ=255)        attaACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGAtt                                                                                                                                 >  4:72953/1‑141 (MQ=255)        attaACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGAtt                                                                                                                                 <  2:615988/141‑1 (MQ=255)             cacCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCtaa                                                                                                                            >  1:242099/1‑141 (MQ=255)              acCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCtaat                                                                                                                           >  2:471504/1‑141 (MQ=255)                  tttGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCtaattaat                                                                                                                       <  2:392900/141‑1 (MQ=255)                     gTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGa                                                                                                                    >  1:4132/1‑141 (MQ=255)                      tCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGat                                                                                                                   <  1:253052/141‑1 (MQ=255)                      tCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGat                                                                                                                   >  3:129833/1‑141 (MQ=255)                           tGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCta                                                                                                                             >  1:117036/1‑126 (MQ=255)                           tGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCta                                                                                                                             <  2:117036/126‑1 (MQ=255)                           tGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCt                                                                                                              <  2:381947/141‑1 (MQ=255)                           tGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCt                                                                                                              >  1:404811/1‑141 (MQ=255)                              ccTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGa                                                                                                           >  2:127099/1‑141 (MQ=255)                                 aTCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATcc                                                                                                        >  2:377423/1‑141 (MQ=255)                                  tCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCa                                                                                                       <  1:230148/141‑1 (MQ=255)                                   cGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCaa                                                                                                      <  3:111735/141‑1 (MQ=255)                                   cGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCaa                                                                                                      <  2:49330/141‑1 (MQ=255)                                    gCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAAt                                                                                                     <  1:416820/141‑1 (MQ=255)                                    gCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAAt                                                                                                     <  2:37363/141‑1 (MQ=255)                                       aTCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCa                                                                                                  <  2:559001/141‑1 (MQ=255)                                           tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTa                                                                                              >  2:462855/1‑141 (MQ=255)                                           tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTa                                                                                              <  1:143889/141‑1 (MQ=255)                                           tatTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTa                                                                                              <  2:51050/141‑1 (MQ=255)                                            atTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTaa                                                                                             >  1:554344/1‑141 (MQ=255)                                            atTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTaa                                                                                             <  1:96265/141‑1 (MQ=255)                                            atTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTaa                                                                                             >  3:162139/1‑141 (MQ=255)                                              ttGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAAt                                                                                           <  3:130952/141‑1 (MQ=255)                                               tGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATg                                                                                          <  2:554344/141‑1 (MQ=255)                                               tGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATATGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATg                                                                                          >  2:321750/1‑141 (MQ=255)                                                 ttAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAc                                                                                        >  4:238787/1‑141 (MQ=255)                                                 ttAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAc                                                                                        <  3:238787/141‑1 (MQ=255)                                                   aaTGTTCCAATCCTTGGTCTGATAACTTGATTGATTTACGGAATGATTTGTATGCTTTCCATTGGGTGTGCTTGTACAATCTGTCTGTTTTCAATATTTCTAATTAATTTAAGATCTTGCTCCATTTCATTTAAATGAC‑tt                                                                                     <  4:277044/141‑1 (MQ=255)                                                       ttCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTg                                                                                 <  1:365440/141‑1 (MQ=255)                                                          cAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCAc                                                                              <  2:204306/141‑1 (MQ=255)                                                          cAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCAc                                                                              <  2:329111/141‑1 (MQ=255)                                                            aaCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCt                                                                            <  1:285263/141‑1 (MQ=255)                                                            aaCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCt                                                                            >  1:553776/1‑141 (MQ=255)                                                             aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTg                                                                           >  2:419787/1‑141 (MQ=255)                                                             aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTg                                                                           <  2:299892/141‑1 (MQ=255)                                                             aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTg                                                                           <  1:325451/141‑1 (MQ=255)                                                             aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTg                                                                           <  4:26657/141‑1 (MQ=255)                                                              ccTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGt                                                                          >  2:163091/1‑141 (MQ=255)                                                              ccTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGt                                                                          <  1:640086/141‑1 (MQ=255)                                                                ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        <  2:293089/141‑1 (MQ=255)                                                                ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        <  1:18043/141‑1 (MQ=255)                                                                ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        <  4:242704/141‑1 (MQ=255)                                                                ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        <  1:126711/141‑1 (MQ=255)                                                                  ggACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcgcg                                                                      >  2:413036/1‑141 (MQ=255)                                                                  ggACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcgcg                                                                      <  4:161949/141‑1 (MQ=255)                                                                  ggACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcgcg                                                                      >  1:4024/1‑141 (MQ=255)                                                                  ggACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcgcg                                                                      >  1:383029/1‑141 (MQ=255)                                                                     cTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTa                                                                   >  2:7081/1‑141 (MQ=255)                                                                        ataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATg                                                                <  2:308454/141‑1 (MQ=255)                                                                        ataaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATg                                                                <  2:242099/141‑1 (MQ=255)                                                                         taaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGa                                                               <  1:23293/141‑1 (MQ=255)                                                                         taaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGa                                                               <  1:307352/141‑1 (MQ=255)                                                                          aaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCGGTCGCGTTAATGac                                                              <  3:135628/141‑1 (MQ=255)                                                                           aCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGaca                                                             <  4:209631/141‑1 (MQ=255)                                                                           aCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGaca                                                             <  1:471504/141‑1 (MQ=255)                                                                                aTTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACAtt                                                        <  1:134108/141‑1 (MQ=255)                                                                                  tGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATtat                                                      <  1:462855/141‑1 (MQ=255)                                                                                   ggTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATtata                                                     <  4:158147/141‑1 (MQ=255)                                                                                             aTGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACt                                           <  2:4132/141‑1 (MQ=255)                                                                                              tGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACt                                           >  2:235518/1‑141 (MQ=255)                                                                                              tGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTg                                          >  3:37684/1‑141 (MQ=255)                                                                                               gATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGa                                         <  2:66667/141‑1 (MQ=255)                                                                                                  ttGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTc                                      <  1:467395/141‑1 (MQ=255)                                                                                                  ttGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTc                                      <  4:124612/141‑1 (MQ=255)                                                                                                   tGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCCTCTTGATCTAATTCATTTAAATGAC‑GTTTTGCACCTGTCGCGTGAATGAGAATATGAAATATGTCTAGTGGTTCt                                     >  2:324877/1‑141 (MQ=255)                                                                                                     tATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTg                                   <  1:180822/141‑1 (MQ=255)                                                                                                         ccTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGttt                               >  3:126475/1‑141 (MQ=255)                                                                                                            cccATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        >  2:234353/1‑97 (MQ=255)                                                                                                            cccATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTcg                                                                        <  1:234353/97‑1 (MQ=255)                                                                                                             ccATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGt                           <  1:7081/141‑1 (MQ=255)                                                                                                                ttGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTTCTTCTTTCTCGTTAATGACAACATTATATATGTCTCCTGATTCTTGGTTTTTGTATg                        >  2:526058/1‑141 (MQ=255)                                                                                                                 tGGGTGTNCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGa                       <  2:483532/141‑1 (MQ=255)                                                                                                                   ggTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaa                     >  3:112986/1‑141 (MQ=255)                                                                                                                    gtgtGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAGCATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                    <  2:553776/141‑1 (MQ=255)                                                                                                                    gtgtGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                    >  3:154611/1‑141 (MQ=255)                                                                                                                      gtgCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaat                  >  1:408342/1‑141 (MQ=255)                                                                                                                        gCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataa                >  4:67189/1‑141 (MQ=255)                                                                                                                         cTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataat               >  1:364515/1‑141 (MQ=255)                                                                                                                                   ttGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGTTTCTTGGTATTAATAAAAAAAATAATACAACTTGcc     <  2:63306/141‑1 (MQ=255)                                                                                                                                    tGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCca    <  2:4024/141‑1 (MQ=255)                                                                                                                                     gTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCcat   >  2:403672/1‑141 (MQ=255)                                                                                                                                      tCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGAAAAAAAAAAAAAAATAATACAACTTGCcatc  <  4:37684/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           GTAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAC‑TTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATC  >  USA300TCH1516_ALE/1899606‑1899879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑