Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,722,719	C→T	G13D (GGT→GAT)	yciC_3 ←	Putative metal chaperone YciC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,722,719	0	C	T	100.0%	324.9 / NA	87	G13D (GGT→GAT)	yciC_3	Putative metal chaperone YciC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/41);  total (46/41)

TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  USA300TCH1516_ALE/2722582‑2722845                                                                                                                                          |                                                                                                                               tCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGc                                                                                                                             <  1:107472/141‑1 (MQ=255)   aGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCa                                                                                                                           <  1:42839/141‑1 (MQ=255)   aGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATCATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGACCAAATGATTTAAGACAGTCGTTTTATCGcta                                                                                                                           >  4:138526/1‑139 (MQ=255)    gTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCAc                                                                                                                          >  1:514424/1‑141 (MQ=255)         cTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaa                                                                                                                     >  3:249420/1‑141 (MQ=255)         cTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaa                                                                                                                     <  2:230359/141‑1 (MQ=255)              aGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAAccc                                                                                                                >  2:282065/1‑141 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCATATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  2:199919/1‑141 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        <  4:12027/128‑1 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        >  3:11982/1‑128 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        >  3:12027/1‑128 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        <  4:11982/128‑1 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaaaa                                                                                                                   <  3:27736/133‑1 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaaaa                                                                                                                   >  4:27736/1‑133 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:587263/1‑141 (MQ=255)                   tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:92160/1‑141 (MQ=255)                     tCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         <  3:183963/141‑1 (MQ=255)                      cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:206221/141‑1 (MQ=255)                      cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  4:100647/141‑1 (MQ=255)                      cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:18356/141‑1 (MQ=255)                      cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:21774/141‑1 (MQ=255)                       ttCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataat                                                                                                       <  1:295991/141‑1 (MQ=255)                          aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         >  4:165231/1‑136 (MQ=255)                          aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         <  3:165231/136‑1 (MQ=255)                          aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAAc                                                                                                    >  4:119618/1‑141 (MQ=255)                             tACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAt                                                                                                 <  1:191498/141‑1 (MQ=255)                              aCTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtt                                                                                                >  3:7100/1‑141 (MQ=255)                                tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:95848/1‑141 (MQ=255)                                tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  4:56015/1‑141 (MQ=255)                                tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:600232/1‑141 (MQ=255)                                tAAGGCACCATCAACACNCATTTTCCCANATTCNTTCNTGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:394922/1‑141 (MQ=255)                                    gCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATc                                                                                          >  4:81388/1‑141 (MQ=255)                                     cACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCt                                                                                         <  2:365809/141‑1 (MQ=255)                                       ccATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGAAAAATAAAAACCCACCTATAATAACTATTTTCATCTGc                                                                                       <  2:519561/141‑1 (MQ=255)                                          tCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGAc                                                                                    >  4:91339/1‑141 (MQ=255)                                           cAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTAAGCGATTCCTTTAATGACTAAGAGAGCATATGACTTAAGACAGTCGTTATTTAGTAATTAATAAAAAGCCCTATAATAACTATTTTCATCTGCGAct                                                                                   >  1:94480/1‑141 (MQ=255)                                            aaCACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGActc                                                                                  <  2:587263/141‑1 (MQ=255)                                              cacTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACtctc                                                                                >  4:249076/1‑141 (MQ=255)                                                cTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCCTCTGCGACTctcct                                                                              >  1:134744/1‑141 (MQ=255)                                                    ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          <  1:281544/141‑1 (MQ=255)                                                    ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          >  2:330489/1‑141 (MQ=255)                                                    ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          <  3:56015/141‑1 (MQ=255)                                                     tttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTtat                                                                         <  3:81388/141‑1 (MQ=255)                                                        cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               <  2:579710/132‑1 (MQ=255)                                                        cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               >  1:579710/1‑132 (MQ=255)                                                        cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACt                                                                      >  1:200784/1‑141 (MQ=255)                                                         ccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACtt                                                                     <  1:639664/141‑1 (MQ=255)                                                          cAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTc                                                                    <  4:161493/141‑1 (MQ=255)                                                                   tcatGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATa                                                           >  3:126505/1‑141 (MQ=255)                                                                   tcatGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATa                                                           >  1:559908/1‑141 (MQ=255)                                                                     atgatgACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGc                                                         <  3:119618/141‑1 (MQ=255)                                                                         tgaCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCatat                                                     <  2:407900/141‑1 (MQ=255)                                                                           aCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTc                                                   >  1:129569/1‑141 (MQ=255)                                                                           aCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTc                                                   <  4:186843/141‑1 (MQ=255)                                                                            ctgctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTcc                                                  >  2:332595/1‑141 (MQ=255)                                                                               ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:91409/141‑1 (MQ=255)                                                                               ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:136773/141‑1 (MQ=255)                                                                               ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:179531/141‑1 (MQ=255)                                                                                    ttCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagataga                                          <  2:394922/141‑1 (MQ=255)                                                                                       aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            <  1:3143/136‑1 (MQ=255)                                                                                       aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            >  2:3143/1‑136 (MQ=255)                                                                                       aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGAtct                                       <  3:105213/141‑1 (MQ=255)                                                                                        gCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGAtctc                                      >  4:120754/1‑141 (MQ=255)                                                                                          gATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCt                                    <  1:170422/141‑1 (MQ=255)                                                                                            ttCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGcc                                                                                                                            >  3:109808/1‑51 (MQ=255)                                                                                            ttCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGcc                                                                                                                            <  4:109808/51‑1 (MQ=255)                                                                                              ccTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtg                                >  1:219011/1‑141 (MQ=255)                                                                                               cTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtga                               <  2:129569/141‑1 (MQ=255)                                                                                                tttAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtgac                              >  1:523109/1‑141 (MQ=255)                                                                                                         tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90016/1‑141 (MQ=255)                                                                                                         tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90076/1‑141 (MQ=255)                                                                                                         tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:189611/1‑141 (MQ=255)                                                                                                         tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90005/1‑141 (MQ=255)                                                                                                            gCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGTCATGActct                  <  4:132852/141‑1 (MQ=255)                                                                                                                gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATCATAACTATTTTCATCTGCGCCTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  1:569173/141‑1 (MQ=255)                                                                                                                gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  2:200784/141‑1 (MQ=255)                                                                                                                gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              >  2:625810/1‑141 (MQ=255)                                                                                                                 cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 <  1:380379/137‑1 (MQ=255)                                                                                                                 cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 >  2:380379/1‑137 (MQ=255)                                                                                                                 cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATAtt             <  2:594180/141‑1 (MQ=255)                                                                                                                  aaaTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTc            >  1:507220/1‑141 (MQ=255)                                                                                                                  aaaTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTc            >  3:204220/1‑141 (MQ=255)                                                                                                                    aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAg          <  1:330489/141‑1 (MQ=255)                                                                                                                    aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATACATCTCCTATTGACTGACATGACTCTATATTCAg          >  2:121869/1‑141 (MQ=255)                                                                                                                           aaGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGt   >  2:137047/1‑141 (MQ=255)                                                                                                                            aGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGtt  >  3:278024/1‑141 (MQ=255)                                                                                                                            aGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGtt  >  1:624237/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                               TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  USA300TCH1516_ALE/2722582‑2722845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑