BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	26,506	C→G	R281G (CGC→GGC)	walK →	Sensor protein kinase WalK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	26,506	0	C	G	100.0%	407.1 / NA	112	R281G (CGC→GGC)	walK	Sensor protein kinase WalK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (58/54); total (58/54)

Only 100 of 112 total aligned reads displayed.

AAATTGGCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACCGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAGAGAA  >  USA300TCH1516_ALE/26372‑26642

aaaTTGGCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTg                                                                                                                                    >  2:346891/1‑141 (MQ=255)

    cggCGAATTAGCCTTCGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCGAATATTGAAAGTGAGGAACGTAGACTGGACTCAGTTATCGCCCCTATGAGTGATGGTATTATTGCAACCGACGGCCGTGGACg                                                                                                                                <  2:501460/140‑1 (MQ=255)

     ggCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGt                                                                                                                               <  3:57930/141‑1 (MQ=255)

     ggCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGt                                                                                                                               >  1:97419/1‑141 (MQ=255)

     ggCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGt                                                                                                                               >  2:624604/1‑141 (MQ=255)

             aGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGCCTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTAt                                                                                                                       >  4:168264/1‑141 (MQ=255)

               gtttATCATTTAATAACTTGTCTAGGCGTGTAGAAGAGGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGAACTCAGTTATCACCCATAGGGGTGATGGTATTATTGCAACAGACGGCCGTAGACGTATTCGTATCg                                                                                                                     <  2:60711/140‑1 (MQ=255)

                tttAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGt                                                                                                                    <  2:556974/141‑1 (MQ=255)

                 ttAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTc                                                                                                                   <  1:510202/141‑1 (MQ=255)

                 ttAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTc                                                                                                                   <  2:401693/141‑1 (MQ=255)

                  tAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCa                                                                                                                  <  2:269902/141‑1 (MQ=255)

                         taataaCTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGatat                                                                                                           >  1:226517/1‑141 (MQ=255)

                            taaCTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGc                                                                                                        >  2:450846/1‑141 (MQ=255)

                             aaCTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCa                                                                                                       <  1:455570/141‑1 (MQ=255)

                               cTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACt                                                                                                     >  3:31310/1‑141 (MQ=255)

                                ttGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTGGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTc                                                                                                    <  3:150422/141‑1 (MQ=255)

                                ttGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGatat                                                                                                           <  4:277695/134‑1 (MQ=255)

                                 tGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCa                                                                                                   <  1:617183/141‑1 (MQ=255)

                                 tGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCa                                                                                                   <  1:457765/141‑1 (MQ=255)

                                   tCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAg                                                                                                 <  4:269552/141‑1 (MQ=255)

                                   tCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAg                                                                                                 <  2:417203/141‑1 (MQ=255)

                                   tCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAg                                                                                                 >  1:534483/1‑141 (MQ=255)

                                     tAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGag                                                                                               <  1:609935/141‑2 (MQ=255)

                                        aCGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCt                                                                                            <  4:133508/141‑1 (MQ=255)

                                            gtACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGt                                                                                        >  2:215080/1‑141 (MQ=255)

                                             tACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTa                                                                                       <  1:432656/141‑1 (MQ=255)

                                                aagaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATgg                                                                                    >  2:615610/1‑141 (MQ=255)

                                                 agaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGc                                                                                   >  2:103798/1‑141 (MQ=255)

                                                 agaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGc                                                                                   >  3:69304/1‑141 (MQ=255)

                                                 agaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGc                                                                                   <  1:624604/141‑1 (MQ=255)

                                                  gaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCg                                                                                  <  4:216605/141‑1 (MQ=255)

                                                   aagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAACCGGGCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGa                                                                                 >  3:201294/1‑141 (MQ=255)

                                                   aagCGAAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGa                                                                                 <  4:14029/141‑1 (MQ=255)

                                                    agCGCAGGCTAATACTGAAAGGGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTTCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGaa                                                                                <  4:17299/141‑1 (MQ=255)

                                                       gcAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaaga                                                                             >  2:426801/1‑141 (MQ=255)

                                                       gcAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaaga                                                                             >  1:8510/1‑141 (MQ=255)

                                                        cAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaagaa                                                                            >  3:20247/1‑141 (MQ=255)

                                                        cAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaagaa                                                                            <  2:461845/141‑1 (MQ=255)

                                                         aGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaagaag                                                                           >  4:25766/1‑141 (MQ=255)

                                                          ggCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAagaaga                                                                          >  2:239993/1‑141 (MQ=255)

                                                           gCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGAc                                                                         <  4:20247/141‑1 (MQ=255)

                                                                  cTGAAAGTGAGAAACGTAGACTGTACTCAGTTATCGCCTATGTGGGTTATTTGATTTTTTTAACATTCGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                  <  3:236033/141‑1 (MQ=255)

                                                                  cTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCCCTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                  <  4:150103/141‑1 (MQ=255)

                                                                  cTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGAt                                                                                               <  2:230629/112‑1 (MQ=255)

                                                                  cTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGAt                                                                                               >  1:230629/1‑112 (MQ=255)

                                                                    gAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGACAGAAGAcatcagctga                                                                >  4:150276/1‑136 (MQ=255)

                                                                       aGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGatat                                                             >  3:165446/1‑141 (MQ=255)

                                                                        gTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCCTCGGATAtt                                                            <  1:567687/141‑1 (MQ=255)

                                                                          gagaAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                  <  1:568652/133‑1 (MQ=255)

                                                                          gagaAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                  >  2:568652/1‑133 (MQ=255)

                                                                            gaAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACAt                                                        >  2:446190/1‑141 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATg                                                                                                          <  3:47452/89‑1 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATg                                                                                                          >  4:47452/1‑89 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCCAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGt                                                      >  2:564049/1‑141 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGt                                                      <  1:605055/141‑1 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGt                                                      >  4:121931/1‑141 (MQ=255)

                                                                              aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGt                                                      >  2:450400/1‑141 (MQ=255)

                                                                                cGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTa                                                    <  2:455240/141‑1 (MQ=255)

                                                                                cGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTa                                                    <  1:450400/141‑1 (MQ=255)

                                                                                 gTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTaa                                                   <  2:559254/141‑1 (MQ=255)

                                                                                     aCTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTAGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAgtgt                                               >  1:82543/1‑141 (MQ=255)

                                                                                       tGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTAt                                             <  1:331386/141‑1 (MQ=255)

                                                                                            tCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGt                                        <  3:219498/141‑1 (MQ=255)

                                                                                             cAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGACAGACGAACTCCTCGGATATTACATGTTAAGTGTATTAAGTc                                       >  3:266519/1‑141 (MQ=255)

                                                                                                ttATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTg                                    <  1:635628/141‑1 (MQ=255)

                                                                                                ttATCACCCATATGAGTGATAGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTAtt                                            >  1:595562/1‑133 (MQ=255)

                                                                                                ttATCACCCATATGAGTGATAGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTAtt                                            <  2:595562/133‑1 (MQ=255)

                                                                                                  aTCACTCATATGGGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGaa                                  >  3:94313/1‑141 (MQ=255)

                                                                                                  aTCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGaa                                  >  2:5026/1‑141 (MQ=255)

                                                                                                     aCCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGAt                               >  2:549908/1‑141 (MQ=255)

                                                                                                     aCCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGAt                               >  2:498852/1‑141 (MQ=255)

                                                                                                     aCCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGAt                               >  3:88824/1‑141 (MQ=255)

                                                                                                         atatGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAAt                           >  2:168302/1‑141 (MQ=255)

                                                                                                         atatGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAAt                           >  2:168230/1‑141 (MQ=255)

                                                                                                         atatGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCAGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAAt                           <  1:239993/141‑1 (MQ=255)

                                                                                                         atatGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCCTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTGGAAGATGAAt                           >  1:113271/1‑141 (MQ=255)

                                                                                                          tatGAGTGATGGTATTATTTTAAGATACGGGATTAGTCGTATTCGTATCTTTAATGATATGGCACTAAAGATGCTTTATATGGCGAAATAAGACATCATCAGATAATACATATTAAGTGTATTTGGTCTTGAACATGAAtt                          <  4:100812/141‑1 (MQ=255)

                                                                                                          tatGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAAtt                          <  1:559282/141‑1 (MQ=255)

                                                                                                           atGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAAttt                         <  1:573799/141‑1 (MQ=255)

                                                                                                              aGTGATGGTATTATTGCAATAGACGGCCGTGGACGTATTCGTATCGTCAAGGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGGTGAATTTaaa                      >  4:56820/1‑141 (MQ=255)

                                                                                                              aGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTaaa                      >  1:147122/1‑141 (MQ=255)

                                                                                                               gTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGGTAAGTGTATTAAGTCTTGAAGATGAATTTAAAc                     >  1:142036/1‑141 (MQ=255)

                                                                                                                tGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACt                    >  1:274166/1‑141 (MQ=255)

                                                                                                                    ggTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaa                <  1:69491/141‑1 (MQ=255)

                                                                                                                     gTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaag               <  2:335935/141‑1 (MQ=255)

                                                                                                                      tattatTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaaga              <  1:495279/141‑1 (MQ=255)

                                                                                                                       attattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaaga              >  4:33513/1‑140 (MQ=255)

                                                                                                                       attattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaaga              <  3:33513/140‑1 (MQ=255)

                                                                                                                        ttattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGaaa            >  2:473764/1‑141 (MQ=255)

                                                                                                                         tattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAAt           >  2:503113/1‑141 (MQ=255)

                                                                                                                         tattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAAt           >  1:70677/1‑141 (MQ=255)

                                                                                                                             gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa       <  1:549908/141‑1 (MQ=255)

                                                                                                                             gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa       <  4:225960/141‑1 (MQ=255)

                                                                                                                             gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa       <  2:608792/141‑1 (MQ=255)

                                                                                                                             gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa       >  3:199893/1‑141 (MQ=255)

                                                                                                                              cAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAGACTGGAAGAAATTCAag      >  1:394194/1‑141 (MQ=255)

                                                                                                                               aaCAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAaga     >  2:64463/1‑141 (MQ=255)

                                                                                                                                 cAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAgaga   <  1:479413/141‑1 (MQ=255)

                                                                                                                                 cAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTAAATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAgaga   >  4:114657/1‑141 (MQ=255)

                                                                                                                                  aGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAGAGaa  >  1:323153/1‑141 (MQ=255)

AAATTGGCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACCGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAGAGAA  >  USA300TCH1516_ALE/26372‑26642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`