Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	603,961	G→A	M464I (ATG→ATA)	araB →	Ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	603,961	0	G	A	100.0%	329.2 / NA	88	M464I (ATG→ATA)	araB	Ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/51);  total (37/51)

ACTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGAAAA  >  USA300TCH1516_ALE/603824‑604095                                                                                                                                          |                                                                                                                                       aCTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGAt                                                                                                                                     <  2:254087/141‑1 (MQ=255)  cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    >  1:154397/1‑141 (MQ=255)  cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    >  1:56141/1‑141 (MQ=255)  cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTAATGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    <  1:576932/141‑1 (MQ=255)   tAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTatagatag                                                                                                                                   <  3:15842/141‑1 (MQ=255)       atgatTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAg                                                                                                                               <  1:172555/141‑1 (MQ=255)        tgatTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGt                                                                                                                              >  4:152051/1‑141 (MQ=255)          atTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTAt                                                                                                                            >  1:97360/1‑141 (MQ=255)             aTGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCa                                                                                                                         >  4:184303/1‑141 (MQ=255)              tGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAc                                                                                                                        <  2:535716/141‑1 (MQ=255)               gCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAcc                                                                                                                       <  2:610317/141‑1 (MQ=255)               gCAAAAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAcc                                                                                                                       <  2:610389/141‑1 (MQ=255)                   cAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCa                                                                                                                   >  1:177805/1‑141 (MQ=255)                    aGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCaa                                                                                                                  <  2:318912/141‑1 (MQ=255)                      tATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATa                                                                                                                >  1:32601/1‑141 (MQ=255)                       aTCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAg                                                                                                               >  3:17910/1‑141 (MQ=255)                        tCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAgg                                                                                                              >  3:270550/1‑141 (MQ=255)                          aaGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTACAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGgcg                                                                                                            >  1:176130/1‑141 (MQ=255)                            gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                          <  3:184303/141‑1 (MQ=255)                            gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                          <  2:92749/141‑1 (MQ=255)                            gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                          <  3:272075/141‑1 (MQ=255)                             agtgacATATAGAAATAAAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTGCTGATGGATATCTATGCGAATGCACTGAATAAAAAACTAGTTGTTATAGATAGTGAGTAGGCACCAGCAATAGGCgcag                                                                                                         <  2:154102/136‑1 (MQ=255)                             aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:225894/141‑1 (MQ=255)                             aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:149194/141‑1 (MQ=255)                             aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGCACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:593193/141‑1 (MQ=255)                              ttGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcagc                                                                                                        <  4:78091/141‑1 (MQ=255)                               tGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcagca                                                                                                       <  1:359651/141‑1 (MQ=255)                                ggCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCaa                                                                                                      <  2:40182/141‑1 (MQ=255)                                   atatGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAatat                                                                                                   >  3:142758/1‑141 (MQ=255)                                    tatGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATAtt                                                                                                  <  2:359120/141‑1 (MQ=255)                                      tGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAg                                                                                                <  1:639791/141‑1 (MQ=255)                                            tAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcag                                                                                          <  2:412646/141‑1 (MQ=255)                                            tAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcag                                                                                          <  2:185014/141‑1 (MQ=255)                                             aGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagt                                                                                         >  4:125083/1‑141 (MQ=255)                                               aaaaGGTACTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAAGAAAAAACTAATTGTTATAGATAGTGAGTATGTACCAGCAATAGGCGCAGCAATATTAGGTGcagtca                                                                                       <  1:554310/141‑1 (MQ=255)                                                 aaGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagtcagt                                                                                     >  1:198225/1‑141 (MQ=255)                                                 aaGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagtcagt                                                                                     <  2:608849/141‑1 (MQ=255)                                                      aTTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGtggtgg                                                                                >  2:309580/1‑141 (MQ=255)                                                        ttGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcg                                                                              <  4:17910/141‑1 (MQ=255)                                                         tGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcgc                                                                             >  1:45345/1‑141 (MQ=255)                                                         tGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcgc                                                                             >  3:36926/1‑141 (MQ=255)                                                                       taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                               >  2:374534/1‑141 (MQ=255)                                                                       taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                               >  2:429038/1‑141 (MQ=255)                                                                       taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                               >  3:10389/1‑141 (MQ=255)                                                                         ccTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAAt                                                             <  2:32601/141‑1 (MQ=255)                                                                         ccTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAAt                                                             <  2:633718/141‑1 (MQ=255)                                                                          cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  1:376699/1‑141 (MQ=255)                                                                          cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  2:51767/1‑141 (MQ=255)                                                                          cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  2:51761/1‑141 (MQ=255)                                                                                    tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTTAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  <  1:104801/141‑1 (MQ=255)                                                                                    tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  <  4:270550/141‑1 (MQ=255)                                                                                    tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  >  1:599861/1‑141 (MQ=255)                                                                                    tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  <  1:429038/141‑1 (MQ=255)                                                                                     gCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGAt                                                 <  2:129412/141‑1 (MQ=255)                                                                                     gCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGAt                                                 >  3:58909/1‑141 (MQ=255)                                                                                      cTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATg                                                <  3:240247/141‑1 (MQ=255)                                                                                       tGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGc                                               <  1:305228/141‑1 (MQ=255)                                                                                            tgatgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGa                                          >  1:642572/1‑141 (MQ=255)                                                                                              atgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGaaa                                        <  1:555879/141‑1 (MQ=255)                                                                                              atgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGaaa                                        <  2:167929/141‑1 (MQ=255)                                                                                                 gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                     <  2:229880/141‑1 (MQ=255)                                                                                                 gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                     <  1:301061/141‑1 (MQ=255)                                                                                                 gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                     <  3:152051/141‑1 (MQ=255)                                                                                                  atatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGc                                    >  2:636048/1‑141 (MQ=255)                                                                                                  atatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGc                                    <  1:368292/141‑1 (MQ=255)                                                                                                  atatCGATGTGAATGTACTGTATAACAAACTAATTGTTATAGATAGTGAGAATGCACAAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGCTGCTATGaaggtgc                                    <  4:185229/141‑6 (MQ=255)                                                                                                   tatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGcc                                   <  4:142758/141‑1 (MQ=255)                                                                                                     tCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAAt                                                                      >  2:329933/1‑104 (MQ=255)                                                                                                     tCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAAt                                                                      <  1:329933/104‑1 (MQ=255)                                                                                                      cTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAt                                >  4:3780/1‑141 (MQ=255)                                                                                                       tATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAtt                               <  2:176130/141‑1 (MQ=255)                                                                                                        aTGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAttt                              >  3:141349/1‑141 (MQ=255)                                                                                                        aTGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAttt                              <  2:325240/141‑1 (MQ=255)                                                                                                          gCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTta                            <  2:262167/141‑1 (MQ=255)                                                                                                           cGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTtat                           <  1:425074/141‑1 (MQ=255)                                                                                                           cGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTtat                           <  1:620306/141‑1 (MQ=255)                                                                                                               tGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGa                       <  2:428661/141‑1 (MQ=255)                                                                                                                gTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGGGGTGGCGCATATAATTCAATTAATCACGCAGTTGATGCTATGAAAGAGCCAATTTTAAACGaa                      >  4:6421/1‑141 (MQ=255)                                                                                                                gTACTGAATAAAAAACTAATTGTAATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaa                      >  4:6428/1‑141 (MQ=255)                                                                                                                 tACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaaa                     <  4:277017/141‑1 (MQ=255)                                                                                                                   cTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAAtt                   <  2:222255/141‑1 (MQ=255)                                                                                                                     gAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTaa                 <  2:45345/141‑1 (MQ=255)                                                                                                                             aaCTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTAAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAg         >  3:60578/1‑141 (MQ=255)                                                                                                                              aCTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGc        >  1:182527/1‑141 (MQ=255)                                                                                                                                 aaTTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGa     >  1:487626/1‑141 (MQ=255)                                                                                                                                 aaTTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGACGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGa     >  4:18732/1‑141 (MQ=255)                                                                                                                                    tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACAt                                                                         >  2:394706/1‑70 (MQ=255)                                                                                                                                    tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACAt                                                                         <  1:394706/70‑1 (MQ=255)                                                                                                                                    tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGaaaa  >  1:2281/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                       ACTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGAAAA  >  USA300TCH1516_ALE/603824‑604095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑