BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	6.0%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	6.0%	301.7 / 4.1	104	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (42/53); new base T (2/5); total (46/58)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 108 total aligned reads displayed.

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110511‑110790

aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                             >  1:937289/1‑141 (MQ=255)

aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATAATAAAGAc                                                                                                                                             >  1:129901/1‑141 (MQ=255)

aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGACGACTTTAAAAACTTGAAAAAATATAAAGAc                                                                                                                                             >  1:908032/1‑141 (MQ=255)

aaaaGATATCCGGTTAAAATGATCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                             <  2:441251/141‑1 (MQ=255)

  aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                           <  2:1063392/141‑1 (MQ=255)

  aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAAGTTGAAAAATTATAAAGACgg                                                                                                                                           <  2:371273/141‑1 (MQ=255)

  aaGATAACCGGTTAAAATGGGCGATAATAAAATCATTCCAACAAAAAAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                           <  1:96832/141‑1 (MQ=255)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                          <  1:552469/141‑1 (MQ=255)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                          >  2:634493/1‑141 (MQ=255)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGga                                                                                                                                          >  2:1067746/1‑141 (MQ=255)

     atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                        <  1:31370/141‑1 (MQ=255)

      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       <  1:78027/141‑1 (MQ=255)

        tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                     >  2:1105520/1‑141 (MQ=255)

             ttAAAATGGTAGATAAGAAAATAATTCCAACAAAAGAAATTAAAGACGAAAAAAACAAAAAAGAAATCGAAAACTTTTAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTTat                                                                                                                                <  2:405916/141‑1 (MQ=17)

              tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAGTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCATTATGGCGACTTTAAAAATTTGAAAAATTATAAAGAAGGAGATATTTCata                                                                                                                               >  1:750852/1‑141 (MQ=255)

                 aaTGGTCGATAATAAAATCATTCCAAGAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  2:743166/1‑141 (MQ=255)

                 aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  1:32220/1‑141 (MQ=255)

                 aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  2:1139926/1‑141 (MQ=255)

                  aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAAt                                                                                                                           >  1:904828/1‑141 (MQ=255)

                     ggcGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCa                                                                                                                        <  2:593817/139‑1 (MQ=255)

                      tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                       <  1:982426/141‑1 (MQ=255)

                       cGAGAATAAAATCATTCCAAAAAAAGAAAATAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAAGATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                      <  2:538320/141‑1 (MQ=255)

                        gATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                     <  1:565125/141‑1 (MQ=255)

                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                    >  2:54741/1‑141 (MQ=255)

                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                    >  2:828919/1‑141 (MQ=255)

                            ataaAATCATTCCAACAAAAGAAATTAGAGCTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                 <  1:744570/141‑1 (MQ=255)

                              aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTTCCg                                                                                                               >  1:83101/1‑141 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              <  2:617361/141‑1 (MQ=255)

                                aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                             >  2:466942/1‑141 (MQ=255)

                                aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTGCATATAATACAGAGGTGCTGAg                                                                                                             >  1:861964/1‑141 (MQ=255)

                                 aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGt                                                                                                            >  2:1111118/1‑141 (MQ=255)

                                  tCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGTCGAGtt                                                                                                           <  2:660046/141‑1 (MQ=255)

                                     ttCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTGAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAtt                                                                                                        >  2:403892/1‑141 (MQ=255)

                                      tCCAACAAAAGAAATTAAAGATGAAAAAAAAAAAAAAGAAATCGCAAAGTTAACGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATATAGAGGGAGATATTTCATATAATACAACGGTGTCGAGTTAtag                                                                                                       >  2:557625/1‑139 (MQ=17)

                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                     <  1:1072580/141‑1 (MQ=255)

                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                     <  1:975185/141‑1 (MQ=255)

                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATAGAAAACATTAAGTTCTTTGTTAAATCTAGCGACATTAAAAATAAGAAACATACTAAAGACCGAGAGATCTCATATAATCCCGCAGTGCAGAGTTATTAgg                                                                                                     >  2:127754/1‑141 (MQ=12)

                                         aacaaAAGAAATTAAAGATGAAAAAATCAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                    <  2:1145659/141‑1 (MQ=255)

                                         aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                    <  1:1139926/141‑1 (MQ=255)

                                              acgAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGTTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  1:934766/139‑1 (MQ=255)

                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  2:797982/141‑1 (MQ=255)

                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  2:76622/141‑1 (MQ=255)

                                              aaGAAATTAAAAATTAAAAAATAAAAAAAGAAATCGAAAACTTTCATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  2:271199/141‑1 (MQ=255)

                                              aaGAAATTAAAAAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  2:76467/141‑1 (MQ=255)

                                                   aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTTAATATaaa                                                                                          >  2:186370/1‑141 (MQ=255)

                                                   aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCATGATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                          >  1:630419/1‑141 (MQ=255)

                                                           tGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                  <  1:55878/141‑1 (MQ=255)

                                                               aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTGTTCGGCTAAATATCAATTAACTAatgat                                                                              <  1:758482/141‑1 (MQ=255)

                                                               aaaaTAAAAAAAAAAATCGAAAATCTTAAGTTCTTCGTGCCATATGGCGACGTTAAAAATTTGACAAATTATAAAGACCGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                              <  1:466894/141‑1 (MQ=14)

                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        <  1:808548/141‑1 (MQ=255)

                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        <  1:1000616/141‑1 (MQ=255)

                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        >  2:817434/1‑141 (MQ=255)

                                                                           gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                  >  2:896551/1‑141 (MQ=255)

                                                                            aacgcGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGGGAAATATCAATTAACTAATGATGAGTATAATGTaa                                                                 <  1:281409/137‑1 (MQ=255)

                                                                             aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTGATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAGCTACACAAACTAATGATGATTATAATGTaaa                                                                <  1:880594/141‑1 (MQ=17)

                                                                                  aaagCTTTAAGTTCTTTGTTCAATATGACGACTTTAAAAATTTGAAAAATTATAACGGCGTAGATATTTCATATACCCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAACCAAt                                                           <  2:605840/141‑1 (MQ=17)

                                                                                  aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  1:373563/141‑1 (MQ=255)

                                                                                   aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                          >  1:222982/1‑141 (MQ=255)

                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATTTAAAACAATTAc                                                        >  1:765004/1‑141 (MQ=255)

                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGCGTAATCTGTCTAGATATCAAATAAATAAAGATGATTAAAATATAAAACAATTat                                                        >  1:453755/1‑140 (MQ=17)

                                                                                      cTTTAAGTTCTTTGATCAATATGGCGACTGTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGACTATAATGTAAAACAATTACg                                                       >  1:638108/1‑141 (MQ=255)

                                                                                              tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                               <  2:109564/141‑1 (MQ=255)

                                                                                              tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGATAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                               <  2:135128/141‑1 (MQ=255)

                                                                                                tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAGTGACTAATGATGATTATAATGTAAAACAAATACGCAAAAgatag                                             >  1:670331/1‑140 (MQ=255)

                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgctatga                                           >  2:709315/1‑141 (MQ=255)

                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                           >  1:818287/1‑141 (MQ=255)

                                                                                                    ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                         <  1:1185264/141‑1 (MQ=255)

                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCGAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                       >  2:858958/1‑141 (MQ=255)

                                                                                                          atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                   >  1:82954/1‑141 (MQ=255)

                                                                                                          atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                   >  2:1063179/1‑141 (MQ=255)

                                                                                                            ggCAACTTTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTACAATGTAAAACAATTACGCAAAAGACATTATATACccacc                                 <  2:553711/141‑2 (MQ=17)

                                                                                                             gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                >  1:1108022/1‑141 (MQ=255)

                                                                                                                 ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  1:884697/140‑1 (MQ=11)

                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  1:720834/141‑1 (MQ=255)

                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  2:517014/141‑1 (MQ=255)

                                                                                                                  tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                           <  1:146593/141‑1 (MQ=11)

                                                                                                                    tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAACAGATATGATAAAACAGCGAGTAAAGc                         >  2:437124/1‑141 (MQ=17)

                                                                                                                     aaaaaTTTGAATAATTATAAATACGGAGATATTTCATATAATCCAGAGATGCCGAGTTATTCGGCTAAATGACAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGTTATACCAACAAGTaaacat                        >  2:519494/1‑138 (MQ=14)

                                                                                                                     aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTCATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACGGACGAGTAAAGCt                        >  2:1090676/1‑141 (MQ=255)

                                                                                                                     aaaaaTGTGAAAAAGTAAAAAGACGGAGATATTTCATATAATCTAGTGCAACAGGATTAGTCGTCAAACTATCAATATTCTTAAGATTATTATATTTTAAAACAATTCCGCAAATGAGATGATATACCGACGAGTAAAGCt                        <  2:386904/141‑1 (MQ=12)

                                                                                                                          ttttAAAAATTATAAAGACGGAGATATTTCATTTAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                   <  2:187520/141‑1 (MQ=255)

                                                                                                                          tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                   <  1:41591/141‑1 (MQ=255)

                                                                                                                             gAAAAATTATAAAGACTGACATATTTCATATAATCCAGAGGTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                <  2:302464/141‑1 (MQ=255)

                                                                                                                             gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                >  1:455551/1‑141 (MQ=255)

                                                                                                                             aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                <  2:1031988/137‑1 (MQ=255)

                                                                                                                             aaaaaaTTATAAAGACGGAGATATTTCATATAATCCAGGGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                <  1:1161156/140‑1 (MQ=255)

                                                                                                                              aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACGGACGAGTAAAGCTCCAAAGTTa               >  1:866766/1‑141 (MQ=255)

                                                                                                                              aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTGAAGCACAAAAGTTa               >  2:436090/1‑141 (MQ=255)

                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              >  2:68795/1‑141 (MQ=255)

                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              >  1:90810/1‑141 (MQ=255)

                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt             >  2:618095/1‑141 (MQ=255)

                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:570150/141‑1 (MQ=255)

                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:1147912/141‑1 (MQ=255)

                                                                                                                                       aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa      <  1:603468/141‑1 (MQ=255)

                                                                                                                                       aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa      <  2:1108022/141‑1 (MQ=255)

                                                                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg     >  2:21085/1‑141 (MQ=255)

                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCATGATGATTATAATGTAAAACAATAACGCAAAAGATATGATATACCGACGTGTAAAGCCCCAAAGTTATTGTTAAAAgg    <  1:775251/141‑1 (MQ=255)

                                                                                                                                           aCGGAGATATTTCATATAATCCAGAGGTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  2:757270/141‑1 (MQ=255)

                                                                                                                                           aCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  1:46915/141‑1 (MQ=255)

                                                                                                                                           aCGGAGATATTTCAAATCATCCTGATTAGCCGATTTAGTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATAGACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  2:536581/141‑1 (MQ=18)

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110511‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`