BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,660	T→A	8.5%	I173I (ATT→ATA)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,660	0	T	A	8.5%	308.2 / 15.7	105	I173I (ATT→ATA)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (45/50); new base A (3/6); total (49/56)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 105 total aligned reads displayed.

TTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  USA300TCH1516_ALE/110524‑110798

ttAAAATGGTAGATAAGAAAATAATTCCAACAAAAGAAATTAAAGACGAAAAAAACAAAAAAGAAATCGAAAACTTTTAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTTat                                                                                                                                        <  2:405916/141‑1 (MQ=17)

 tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAGTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCATTATGGCGACTTTAAAAATTTGAAAAATTATAAAGAAGGAGATATTTCata                                                                                                                                       >  1:750852/1‑141 (MQ=255)

    aaTGGTCGATAATAAAATCATTCCAAGAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                                    >  2:743166/1‑141 (MQ=255)

    aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                                    >  1:32220/1‑141 (MQ=255)

     aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAAt                                                                                                                                   >  1:904828/1‑141 (MQ=255)

        ggcGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCa                                                                                                                                <  2:593817/139‑1 (MQ=255)

         tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                               <  1:982426/141‑1 (MQ=255)

          cGAGAATAAAATCATTCCAAAAAAAGAAAATAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAAGATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                              <  2:538320/141‑1 (MQ=255)

           gATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                             <  1:565125/141‑1 (MQ=255)

            ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                            >  2:828919/1‑141 (MQ=255)

               ataaAATCATTCCAACAAAAGAAATTAGAGCTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                         <  1:744570/141‑1 (MQ=255)

                  aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                      <  2:617361/141‑1 (MQ=255)

                   aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                                     >  2:466942/1‑141 (MQ=255)

                   aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTGCATATAATACAGAGGTGCTGAg                                                                                                                     >  1:861964/1‑141 (MQ=255)

                    aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGt                                                                                                                    >  2:1111118/1‑141 (MQ=255)

                     tCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGTCGAGtt                                                                                                                   <  2:660046/141‑1 (MQ=255)

                        ttCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTGAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAtt                                                                                                                >  2:403892/1‑141 (MQ=255)

                         tCCAACAAAAGAAATTAAAGATGAAAAAAAAAAAAAAGAAATCGCAAAGTTAACGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATATAGAGGGAGATATTTCATATAATACAACGGTGTCGAGTTAtag                                                                                                               >  2:557625/1‑139 (MQ=17)

                           caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                             <  1:1072580/141‑1 (MQ=255)

                           caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                             <  1:975185/141‑1 (MQ=255)

                           caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATAGAAAACATTAAGTTCTTTGTTAAATCTAGCGACATTAAAAATAAGAAACATACTAAAGACCGAGAGATCTCATATAATCCCGCAGTGCAGAGTTATTAgg                                                                                                             >  2:127754/1‑141 (MQ=12)

                            aacaaAAGAAATTAAAGATGAAAAAATCAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                            <  2:1145659/141‑1 (MQ=255)

                            aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                            <  1:1139926/141‑1 (MQ=255)

                                 acgAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGTTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                       <  1:934766/139‑1 (MQ=255)

                                 aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                       <  2:76622/141‑1 (MQ=255)

                                 aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                       <  2:797982/141‑1 (MQ=255)

                                 aaGAAATTAAAAATTAAAAAATAAAAAAAGAAATCGAAAACTTTCATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                       <  2:271199/141‑1 (MQ=255)

                                 aaGAAATTAAAAAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                       <  2:76467/141‑1 (MQ=255)

                                      aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTTAATATaaa                                                                                                  >  2:186370/1‑141 (MQ=255)

                                      aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCATGATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                                  >  1:630419/1‑141 (MQ=255)

                                              tGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                          <  1:55878/141‑1 (MQ=255)

                                                  aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTGTTCGGCTAAATATCAATTAACTAatgat                                                                                      <  1:758482/141‑1 (MQ=255)

                                                    aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                    >  1:859031/1‑141 (MQ=255)

                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                <  1:808548/141‑1 (MQ=255)

                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                <  1:992955/141‑1 (MQ=255)

                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                <  1:1000616/141‑1 (MQ=255)

                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                >  1:333943/1‑141 (MQ=255)

                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                >  2:817434/1‑141 (MQ=255)

                                                              gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                          >  2:896551/1‑141 (MQ=255)

                                                               aacgcGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGGGAAATATCAATTAACTAATGATGAGTATAATGTaa                                                                         <  1:281409/137‑1 (MQ=255)

                                                                aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTGATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAGCTACACAAACTAATGATGATTATAATGTaaa                                                                        <  1:880594/141‑1 (MQ=17)

                                                                     aaagCTTTAAGTTCTTTGTTCAATATGACGACTTTAAAAATTTGAAAAATTATAACGGCGTAGATATTTCATATACCCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAACCAAt                                                                   <  2:605840/141‑1 (MQ=17)

                                                                     aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                   <  1:373563/141‑1 (MQ=255)

                                                                      aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                  >  1:222982/1‑141 (MQ=255)

                                                                        aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATTTAAAACAATTAc                                                                >  1:765004/1‑141 (MQ=255)

                                                                        aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGCGTAATCTGTCTAGATATCAAATAAATAAAGATGATTAAAATATAAAACAATTat                                                                >  1:453755/1‑140 (MQ=17)

                                                                         cTTTAAGTTCTTTGATCAATATGGCGACTGTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGACTATAATGTAAAACAATTACg                                                               >  1:638108/1‑141 (MQ=255)

                                                                            tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                            <  1:634493/141‑1 (MQ=255)

                                                                                 tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                       <  2:109564/141‑1 (MQ=255)

                                                                                 tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGATAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                       <  2:135128/141‑1 (MQ=255)

                                                                                   tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAGTGACTAATGATGATTATAATGTAAAACAAATACGCAAAAgatag                                                     >  1:670331/1‑140 (MQ=255)

                                                                                     tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgctatga                                                   >  2:709315/1‑141 (MQ=255)

                                                                                     tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                                   >  1:818287/1‑141 (MQ=255)

                                                                                       ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                                 <  1:1185264/141‑1 (MQ=255)

                                                                                         cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCGAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                               >  2:858958/1‑141 (MQ=255)

                                                                                             atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                           >  1:82954/1‑141 (MQ=255)

                                                                                             atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                           >  2:1063179/1‑141 (MQ=255)

                                                                                               ggCAACTTTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTACAATGTAAAACAATTACGCAAAAGACATTATATACccacc                                         <  2:553711/141‑2 (MQ=17)

                                                                                                gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                        >  1:1108022/1‑141 (MQ=255)

                                                                                                    ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                    <  1:884697/140‑1 (MQ=11)

                                                                                                    cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                    <  1:720834/141‑1 (MQ=255)

                                                                                                    cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                    <  2:517014/141‑1 (MQ=255)

                                                                                                     tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                                   <  1:146593/141‑1 (MQ=11)

                                                                                                       tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAACAGATATGATAAAACAGCGAGTAAAGc                                 >  2:437124/1‑141 (MQ=17)

                                                                                                        aaaaaTTTGAATAATTATAAATACGGAGATATTTCATATAATCCAGAGATGCCGAGTTATTCGGCTAAATGACAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGTTATACCAACAAGTaaacat                                >  2:519494/1‑138 (MQ=14)

                                                                                                        aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTCATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACGGACGAGTAAAGCt                                >  2:1090676/1‑141 (MQ=255)

                                                                                                        aaaaaTGTGAAAAAGTAAAAAGACGGAGATATTTCATATAATCTAGTGCAACAGGATTAGTCGTCAAACTATCAATATTCTTAAGATTATTATATTTTAAAACAATTCCGCAAATGAGATGATATACCGACGAGTAAAGCt                                <  2:386904/141‑1 (MQ=12)

                                                                                                         aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGGGTAAAGCTc                               >  2:442929/1‑141 (MQ=255)

                                                                                                             ttttAAAAATTATAAAGACGGAGATATTTCATTTAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                           <  2:187520/141‑1 (MQ=255)

                                                                                                             tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                           <  1:41591/141‑1 (MQ=255)

                                                                                                                gAAAAATTATAAAGACTGACATATTTCATATAATCCAGAGGTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                        <  2:302464/141‑1 (MQ=255)

                                                                                                                gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                        >  1:455551/1‑141 (MQ=255)

                                                                                                                aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                        <  2:1031988/137‑1 (MQ=255)

                                                                                                                aaaaaaTTATAAAGACGGAGATATTTCATATAATCCAGGGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                        <  1:1161156/140‑1 (MQ=255)

                                                                                                                 aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACGGACGAGTAAAGCTCCAAAGTTa                       >  1:866766/1‑141 (MQ=255)

                                                                                                                 aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTGAAGCACAAAAGTTa                       >  2:436090/1‑141 (MQ=255)

                                                                                                                  aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                      >  2:68795/1‑141 (MQ=255)

                                                                                                                  aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                      >  1:90810/1‑141 (MQ=255)

                                                                                                                   aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt                     >  2:618095/1‑141 (MQ=255)

                                                                                                                    aaTTATAAAGATGGAGATGTATCATATAATCCAGAGGTGCCGAGTTATTGGGCTATATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAAATACCGACGAGTAAAGCTCCAAAGTTATTg                    >  2:135907/1‑141 (MQ=17)

                                                                                                                        ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa                <  1:570150/141‑1 (MQ=255)

                                                                                                                        ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa                <  1:1147912/141‑1 (MQ=255)

                                                                                                                          aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa              <  2:1108022/141‑1 (MQ=255)

                                                                                                                          aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa              <  1:603468/141‑1 (MQ=255)

                                                                                                                           aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg             >  2:21085/1‑141 (MQ=255)

                                                                                                                            aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCATGATGATTATAATGTAAAACAATAACGCAAAAGATATGATATACCGACGTGTAAAGCCCCAAAGTTATTGTTAAAAgg            <  1:775251/141‑1 (MQ=255)

                                                                                                                             gatggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           >  1:786217/4‑141 (MQ=255)

                                                                                                                             gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAGATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           <  2:90810/141‑1 (MQ=255)

                                                                                                                             aaCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           <  2:641684/140‑1 (MQ=255)

                                                                                                                              aCGGAGATATTTCATATAATCCAGAGGTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt          <  2:757270/141‑1 (MQ=255)

                                                                                                                              aCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt          <  1:46915/141‑1 (MQ=255)

                                                                                                                              aCGGAGATATTTCAAATCATCCTGATTAGCCGATTTAGTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATAGACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt          <  2:536581/141‑1 (MQ=18)

                                                                                                                                  agaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAACCATCAAAGTTATTGTTAAAAGGTTCAgg      <  1:880135/141‑1 (MQ=255)

                                                                                                                                  agaTATTTCATATAATCCAAAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      >  1:779636/1‑141 (MQ=255)

                                                                                                                                  agaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      <  1:18291/141‑1 (MQ=255)

                                                                                                                                   gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg     <  2:111062/141‑1 (MQ=255)

                                                                                                                                    atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa    >  1:255730/1‑141 (MQ=255)

                                                                                                                                      atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:953734/1‑141 (MQ=255)

                                                                                                                                      atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:143073/1‑141 (MQ=255)

                                                                                                                                      atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:1016925/1‑141 (MQ=255)

TTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  USA300TCH1516_ALE/110524‑110798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`