BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,516	C→A	27.2%	Q196K (CAA→AAA)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,516	0	C	A	27.2%	228.9 / 77.0	127	Q196K (CAA→AAA)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (46/46); new base A (15/20); total (61/66)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  USA300TCH1516_ALE/111379‑111656

aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                           <  1:920575/141‑1 (MQ=255)

 ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                          <  2:432766/141‑1 (MQ=255)

        aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCGGGTAATTAGCAATTa                                                                                                                                   >  2:8834/1‑141 (MQ=18)

         aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                                  >  1:600881/1‑141 (MQ=255)

         aagaagAAAACAAAAAAAAAGAAATAGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATCAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                                  <  1:287185/141‑1 (MQ=18)

          agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                                 >  1:1066626/1‑141 (MQ=255)

           aaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                                                                <  2:119912/140‑1 (MQ=255)

              gaaAACATAAAAAAATAAATCGAAAACTTTAAGTTTTTTGCGCAATATTGCAAATTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGTCTAAATATCAATTAACTAat                                                                                                                             <  1:750066/141‑1 (MQ=17)

              gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                             <  1:84225/141‑1 (MQ=255)

              gaaAACATAAAAAAAGAAAACGTACGCGGTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                             >  2:549720/1‑141 (MQ=21)

               aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGTTATATAGCAATATTAAAAATTTAATGAATAATAAAGATGGAGATATATCATATAAACAAGAGGTGCGGAGTGATTGAGGTAAATATACATTAAGTActg                                                                                                                            >  2:732661/1‑141 (MQ=18)

               aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                            >  2:146593/1‑141 (MQ=255)

               aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                            >  1:395636/1‑141 (MQ=255)

               aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaagg                                                                                                                            <  2:1037397/141‑3 (MQ=255)

               aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatg                                                                                                                            <  2:365442/141‑1 (MQ=255)

                aaaCAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                                                           <  2:339271/141‑1 (MQ=255)

                 aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                          >  1:535511/1‑141 (MQ=255)

                 aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgag                                                                                                                          >  1:211505/1‑140 (MQ=255)

                 aaCAAAAAAAAAGAAAACGAAAACGTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                          <  2:328461/141‑1 (MQ=17)

                     tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTTCAATAAAAAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGACAAAATATCAATAAACAAacgaaaatt                                                                                                                      >  2:631950/1‑136 (MQ=9)

                     tAAAAAAAGAAATCGAAAACGTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAtt                                                                                                                      <  2:1082858/141‑1 (MQ=255)

                     aacaaaaaGAAACCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                      <  1:569645/138‑1 (MQ=255)

                      aaaaaaaGAAATCGAAATCTTTGAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGTGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                                     <  2:582254/141‑1 (MQ=17)

                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAGAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                    >  1:1197182/1‑141 (MQ=255)

                       aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                    <  1:308717/141‑1 (MQ=255)

                       aaaaaaGAAATCGAAAACTTGAAGTTTTTTCCGGAATATAGCAATTTTAAAGAATTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGCTtat                                                                                                                    <  1:815440/141‑1 (MQ=18)

                       aaaaaaGAAATCGAAAACTATAAGTTTTTTGCGAAATATAACAATTTTAAAAAGTTAATGAATTATAAAGATAGAGATATATCATATAATCCAGAGATCACGAATTATTAAGCTCAATATCAATATACTAattatacttaa                                                                                                                    >  2:682172/1‑135 (MQ=18)

                           aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTGATGATTATAATg                                                                                                                <  1:639995/141‑1 (MQ=255)

                             aaaaTCGAAAACTTTAAGTTTGTTGCGCAATATAGCAATTTTAAATATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTa                                                                                                              <  1:288946/140‑1 (MQ=255)

                               aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                            >  1:1106332/1‑141 (MQ=255)

                                aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                           >  1:958714/1‑141 (MQ=255)

                                 tCGAAAACTATAGGTTTTTTGCGCAAAAAGTTGGTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                                                                          >  2:1099614/1‑141 (MQ=14)

                                  cGAGAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATAAATTAATTAATGATGATTATAATGTAAcaca                                                                                                         >  2:72570/1‑141 (MQ=255)

                                  cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                         >  2:1053989/1‑141 (MQ=255)

                                  cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                         >  2:1053964/1‑141 (MQ=255)

                                    aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                       <  1:554082/141‑1 (MQ=255)

                                    aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTCTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGCGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                       <  2:667608/141‑1 (MQ=255)

                                     aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATTCAAATTAAAGAATGATGCTTATGATGTAAAAAAAtt                                                                                                      >  2:861860/1‑141 (MQ=18)

                                       aCTTTAAGTTTGTTGCACAATATAGCAAGTTGAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGGGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                                    <  1:1068461/141‑1 (MQ=18)

                                         tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGt                                                                                                  <  1:398017/141‑1 (MQ=255)

                                         tttAAGTTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgc                                                                                                  <  2:531322/141‑2 (MQ=255)

                                           tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgcaa                                                                                                <  2:769087/141‑4 (MQ=255)

                                           tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                <  2:469999/141‑1 (MQ=255)

                                           tAAGTTTATTGCGCAATATAGCAATTTTAAAGATTTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGACTTTCTCAGCTCATACTCAAATGACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                <  1:1183153/141‑1 (MQ=18)

                                            aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaa                                                                                               <  1:790395/141‑1 (MQ=255)

                                              gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaaa                                                                                             >  2:686026/1‑140 (MQ=255)

                                                tttctGCGCAATATAGCGATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTGTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGat                                                                                           <  2:803511/141‑1 (MQ=255)

                                                  tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGatat                                                                                         >  2:115523/1‑141 (MQ=255)

                                                    tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTTACTAATGATGATTATAATGTAAAACAATTTCGTAAAAGATATGa                                                                                       <  1:656248/141‑1 (MQ=255)

                                                     gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                      <  1:434555/141‑2 (MQ=255)

                                                     gcgcAATATAGCAATCTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATAACGATTATAATGTAAAACAATTACGTAAAAGaccagac                                                                                      >  2:1197972/1‑135 (MQ=18)

                                                      cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGAGATAGCATATAATCCAGAGGTGCCGAGTTATTCAGTTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                     <  1:513580/141‑1 (MQ=255)

                                                      agcAATATAACAATTTTAAAGAGTTAAAGAAATATAAAGATGGAGATATACCATATAATCCAGAGGTGCCGAGTTATTCAGTTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                     <  1:718555/140‑1 (MQ=18)

                                                       gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTTAAACGATATGGCAt                                                                                    >  2:319213/1‑141 (MQ=255)

                                                         aaTATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAc                                                                                  >  2:861343/1‑141 (MQ=255)

                                                           tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                <  2:824053/141‑1 (MQ=255)

                                                             taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaaa                                                                              >  1:790175/1‑140 (MQ=255)

                                                             taGCAATGTTAAAGATTCAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgac                                                                              <  2:802035/141‑4 (MQ=11)

                                                                         aGATTTAATGAATTATAAAGATGGAGATATATCATATACTCCAGATGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                  >  1:525649/1‑141 (MQ=255)

                                                                         aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAACCAATTACGTAAAAGATATAACGAACCAACAAATAAAACGcc                                                                  >  2:223623/1‑141 (MQ=17)

                                                                         aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATGAGGCGcc                                                                  >  1:637958/1‑141 (MQ=255)

                                                                          gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAACTAAAGCGCCg                                                                 >  2:262862/1‑141 (MQ=255)

                                                                          gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                                 >  1:601370/1‑141 (MQ=255)

                                                                           aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                <  2:834414/141‑1 (MQ=255)

                                                                               aaTGAATTATAAAGATGGAGGTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAgat                                                            >  2:1049753/1‑139 (MQ=255)

                                                                               aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                            <  2:47046/141‑1 (MQ=255)

                                                                                aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                           <  1:471009/141‑1 (MQ=255)

                                                                                            gacggAGATATATCACATAAACCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCTGAAGCTGTTGTTGAAAGGt                                               <  1:305226/138‑1 (MQ=255)

                                                                                            gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATGTGACATACCAACAGATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                               <  1:1195126/141‑1 (MQ=255)

                                                                                            gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                               >  1:1128415/1‑141 (MQ=255)

                                                                                            gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                               >  1:1073692/1‑141 (MQ=255)

                                                                                            gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                               >  1:1023025/1‑141 (MQ=255)

                                                                                                 agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                          >  2:797739/1‑141 (MQ=255)

                                                                                                    tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGTGaa                                       <  1:861343/141‑1 (MQ=255)

                                                                                                     atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                                      <  2:740768/141‑1 (MQ=255)

                                                                                                       atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAttt                                    <  1:1080200/141‑1 (MQ=255)

                                                                                                        acATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCAGATTATAAAGTAAAACCATAACATAAAACAAATCACATACAAACAAATCAAGCGCCGAAACTGTTGTTGAAAGGTACAGGGaacata                                   >  2:163896/2‑137 (MQ=14)

                                                                                                         cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                  <  1:111845/141‑1 (MQ=255)

                                                                                                          atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                 <  2:866157/141‑1 (MQ=255)

                                                                                                          atatAATCCAGAGGTGCCGAGTTAATCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                 >  1:872948/1‑141 (MQ=255)

                                                                                                              aaTCCAGAGGTGTCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGGCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                             <  2:814183/141‑1 (MQ=255)

                                                                                                              aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                             <  2:396659/141‑1 (MQ=255)

                                                                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                            <  2:1023025/141‑1 (MQ=255)

                                                                                                                tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                           >  2:455386/1‑141 (MQ=255)

                                                                                                                tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGATGTTGTTGAAAGGTACAGGGAATTTAAAAGGtta                           >  2:992447/1‑140 (MQ=255)

                                                                                                                 gcAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGCAGCTGTTGTTGAAAGGTACAGGGAATTAACAAGGTaca                          >  2:668686/2‑141 (MQ=255)

                                                                                                                 ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAACGCGCCGCAGCTGTTGTAGAAAGGTACAGGAAATTGAAAAGGTtca                          >  1:220007/1‑141 (MQ=17)

                                                                                                                 ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAGTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGTGGTTGAAAGGTAAAGGGAATTTAAAGGGTtcc                          >  2:965258/1‑140 (MQ=17)

                                                                                                                  cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATAAGACATACCAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcct                         >  2:865682/1‑139 (MQ=255)

                                                                                                                    gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAATTTAAAAGGTtcatca                       >  1:596688/1‑141 (MQ=255)

                                                                                                                       gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCGTCAGtt                    >  1:427531/1‑141 (MQ=255)

                                                                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                  <  1:187439/141‑1 (MQ=255)

                                                                                                                             aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat              <  1:780674/141‑1 (MQ=255)

                                                                                                                             aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat              <  1:183187/141‑1 (MQ=255)

                                                                                                                              gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata             >  2:475810/1‑141 (MQ=255)

                                                                                                                              gTTATTCAGCTCAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata             <  2:258799/141‑1 (MQ=255)

                                                                                                                                tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa           >  1:742357/1‑141 (MQ=255)

                                                                                                                                tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa           <  1:685576/141‑1 (MQ=255)

                                                                                                                                tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGCATGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa           <  1:281317/141‑1 (MQ=255)

                                                                                                                                 aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa          >  2:1161368/1‑141 (MQ=255)

AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  USA300TCH1516_ALE/111379‑111656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`