Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 665,392 T→G 19.9% L79* (TTA→TGA)  paiA_1 → Spermidine/spermine N(1)‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE665,3920TG19.9% 239.6 / 65.3 128L79* (TTA→TGA) paiA_1Spermidine/spermine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (54/47);  new base G (14/11);  total (69/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 131 total aligned reads displayed.

TGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCA  >  USA300TCH1516_ALE/665252‑665531
                                                                                                                                            |                                                                                                                                           
tGCGACAACAGTTAATCACTTGTTAGTAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTATTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAtt                                                                                                                                             <  1:722922/141‑1 (MQ=255)
tGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAtt                                                                                                                                             <  2:1174191/141‑1 (MQ=255)
    acaacaGTTAATCACTTGTTAGAAGCTTCATATAATTAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTACTCGTTGAACAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACCGTGAAGAATTatat                                                                                                                                         <  1:930874/141‑1 (MQ=255)
    acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCGTTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                         >  1:919363/1‑141 (MQ=255)
    acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTatat                                                                                                                                         <  1:1119158/141‑1 (MQ=255)
    acaacaGTGAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTatat                                                                                                                                         <  1:159927/141‑1 (MQ=255)
    aaagcaGTTAATAACTTGTTAGAAGCTTCATCTAATGACCATCATTTAAAGAAAAGACTCCAAGAACAATTTTTCTTAATCGTTGACGAAGGTAATGACATAGTTGGCTTTGCTAACTGTATTTACGGTGATGAATTatat                                                                                                                                         >  2:322714/5‑141 (MQ=255)
    aaaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTatat                                                                                                                                         <  2:334848/139‑1 (MQ=255)
      aacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTAGAGAATTATAttt                                                                                                                                       >  2:288144/1‑141 (MQ=255)
       acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTa                                                                                                                                      >  1:218292/1‑141 (MQ=255)
       acaGTTAATCACTTGTTAGAAGCTTCATATAATAAACATCATTTAACGAAAAGACTTCAATAACAATTATTCTTAGTCGTTGAAGAAGGTCATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATAAGATTTa                                                                                                                                      >  2:160726/1‑141 (MQ=255)
           ttAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAg                                                                                                                                  >  1:588460/1‑141 (MQ=255)
            tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTGTATTTATCAGc                                                                                                                                 >  1:60852/1‑141 (MQ=255)
                cACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTAAGGTGAAGAATTATATTTATCAGCTCAt                                                                                                                             <  2:698939/141‑1 (MQ=255)
                cACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTAGATAACTTTATACACGGTGAAGAATAATAATTATCATCTCAt                                                                                                                             <  2:698972/141‑1 (MQ=255)
                  cTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATGTACGGTGAAGAAGTATATTTCTCAGCTCAttc                                                                                                                           >  1:492958/1‑140 (MQ=255)
                   ttGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTAt                                                                                                                          <  2:1084871/141‑1 (MQ=255)
                   ttGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTAt                                                                                                                          <  2:1084997/141‑1 (MQ=255)
                    tGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTCATCAAACAGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATg                                                                                                                         >  1:1176231/1‑141 (MQ=255)
                    tGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATg                                                                                                                         >  2:725322/1‑141 (MQ=255)
                    tGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATg                                                                                                                         <  2:1047916/141‑1 (MQ=255)
                     gTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTTTGt                                                                                                                        >  2:63656/1‑141 (MQ=255)
                      ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                       <  1:415130/141‑1 (MQ=255)
                      ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                       <  1:1021422/141‑1 (MQ=255)
                      ttAGAAGCTTCATAATATGAACATCATTTAAAGAAAAGACTTCAAGCACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                       <  1:1021418/141‑1 (MQ=255)
                        aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaa                                                                                                                     >  1:785007/1‑141 (MQ=255)
                        aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATGCTTAGTCGTGGAAGAAGGTAATGACATCGTTAGCTTTGCTAACTTTATTTACGTTGAAGAATTATCTGTATCAGATAATTATGTTaa                                                                                                                     >  2:552667/1‑141 (MQ=255)
                         gAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaaa                                                                                                                    >  1:968969/1‑141 (MQ=255)
                          aaGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGATCAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAAc                                                                                                                   >  2:1092917/1‑141 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGTTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 <  2:577954/141‑1 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATAATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 <  2:1029309/141‑1 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATAATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 <  2:168641/141‑1 (MQ=255)
                                cATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAAt                                                                                                             <  2:861580/141‑1 (MQ=255)
                                 atatAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                            >  1:316469/1‑141 (MQ=255)
                                   ataATGAACATCATTTAAAGTAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                          >  1:725801/1‑141 (MQ=255)
                                   ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                          <  2:1055336/141‑1 (MQ=255)
                                     aaTGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGGTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCaa                                                                                                        >  1:1178293/1‑141 (MQ=255)
                                       tGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                                      >  2:450666/1‑141 (MQ=255)
                                       tGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                                      >  2:450662/1‑141 (MQ=255)
                                           catcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACa                                                                                                  <  1:365534/141‑1 (MQ=255)
                                            atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                                 >  2:249883/1‑141 (MQ=255)
                                            atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCCAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                                 <  2:1025469/141‑1 (MQ=255)
                                                 ttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                            >  2:572797/1‑141 (MQ=255)
                                                 ttAAAGAAAAGACTTAAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                            >  1:885882/1‑141 (MQ=255)
                                                    aagaaaagaCTTCAAGCACAATGATTCTTTGTCGTTGGTGAAGGTAATGACATCGTTGGCGTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAAGCGCAACATACAGGTTATGGt                                                                                         <  2:680831/141‑1 (MQ=255)
                                                    aagaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGt                                                                                         <  1:787218/141‑1 (MQ=255)
                                                     agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTGTGGTa                                                                                        >  2:711929/1‑141 (MQ=255)
                                                     agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTa                                                                                        >  2:239283/1‑141 (MQ=255)
                                                      gaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                       >  1:368658/1‑141 (MQ=255)
                                                      gaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGGAc                                                                                       >  1:985037/1‑141 (MQ=255)
                                                      aaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACCTCGTGGGCGTTGCTAACTTTATTTACGGCGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                       <  1:928667/140‑1 (MQ=255)
                                                       aaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACGGGTTATGGTACa                                                                                      <  2:638400/141‑1 (MQ=255)
                                                        aaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAGGAATTATATGTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAg                                                                                     >  2:542886/1‑141 (MQ=255)
                                                           gaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTCATCGGCTCATGGTGGTTAACCAGAATCGCAACATACAGGTTATGGTACAGCAt                                                                                  >  1:91912/1‑141 (MQ=255)
                                                            aCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGAAtt                                                                                 >  2:885487/1‑141 (MQ=255)
                                                               tCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGtt                                                                              >  2:1144499/1‑141 (MQ=255)
                                                               tCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGtt                                                                              >  1:683785/1‑141 (MQ=255)
                                                                cAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGGACAGCATTGTTa                                                                             >  1:385928/1‑141 (MQ=255)
                                                                  aGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATTCAGGTTATGGTGCAGCATTGTTaaa                                                                           >  2:316860/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGTAGGGGATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:1157294/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:731070/1‑141 (MQ=255)
                                                                        attattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAgg                                                                     <  2:94137/141‑1 (MQ=255)
                                                                          tattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGAt                                                                   <  2:588460/141‑1 (MQ=255)
                                                                           attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGAATTGTTAAATGAAGGAtt                                                                  >  2:228525/1‑141 (MQ=255)
                                                                           attCTTAGTCGTTGAAGAAGGTAACGACATCGTTGGCTGTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACAAGAATCCCAACATACAGGTTATGGTACAGCACTGTTAAATGAAGGAtt                                                                  <  1:821681/141‑1 (MQ=255)
                                                                              cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGGTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATc                                                               <  2:93190/141‑1 (MQ=255)
                                                                                  gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                           <  1:1091256/141‑1 (MQ=255)
                                                                                        gaagaaGGTAATGACATCGTTGGCTTTGGCAACGGTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGaa                                                     >  1:577271/1‑141 (MQ=255)
                                                                                        gaagaaGGTAATGACATCGTTGGCTTTGCTAACTTTATTTAGGGTGAAGCATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGttttcgt                                                     <  2:12199/141‑4 (MQ=255)
                                                                                           aaagtttaTGACATCGTTGGCTTTGCTAACTGTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAAATACCGTTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                                  <  1:743547/134‑1 (MQ=255)
                                                                                            aagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATa                                                 >  2:66878/1‑141 (MQ=255)
                                                                                            aagGTAATGACATCGTTGACTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATa                                                 >  2:758072/1‑141 (MQ=255)
                                                                                              ggTAATGGCTTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATaaa                                               <  2:951804/141‑1 (MQ=255)
                                                                                              ggTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATgaa                                               >  1:1193905/1‑141 (MQ=255)
                                                                                              ggTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATaaa                                               <  1:187125/141‑1 (MQ=255)
                                                                                                  aTGACATCGTTGGCTTTGCTAACTTTATTTAAGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttgg                                           >  2:225220/1‑139 (MQ=255)
                                                                                                   tGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGa                                          <  1:159342/141‑1 (MQ=255)
                                                                                                    gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                         <  2:373271/141‑1 (MQ=255)
                                                                                                    gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                         <  1:239283/141‑1 (MQ=255)
                                                                                                      cATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgg                                       >  2:473544/1‑141 (MQ=255)
                                                                                                       aTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTCTTAAATGACGGATTATCACATTTTGAAGATAAATTTGAAgtt                                      >  2:459272/1‑139 (MQ=255)
                                                                                                        tCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCCGCCCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgggg                                     >  1:1065875/1‑139 (MQ=255)
                                                                                                         cGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgtgt                                    >  1:26357/1‑141 (MQ=255)
                                                                                                          gTTGGCTTTGCTAACTTTATTTAGGGTTAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAAATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGGTAAATTGGAAggagat                                   >  2:39489/1‑137 (MQ=255)
                                                                                                          gTTGGCTTTGCTAACGTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGATAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGtt                                   <  2:639189/141‑1 (MQ=255)
                                                                                                               cTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGCTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACtt                              <  1:356520/141‑1 (MQ=255)
                                                                                                               cTTTGCTAACTTTATTGACGGTGCAGAATGATATTTATCAGCTCATCATGTTAAACCAGAATCGAAACATACAGGTTATGGTAGAGCATTGTGAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACtt                              <  1:258968/141‑1 (MQ=255)
                                                                                                                tttGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTa                             >  1:535825/1‑141 (MQ=255)
                                                                                                                tttGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTGAAATGAAGGATTATCACGTTTTGAAGATAAGTTTGAAGGTGTTTACTTa                             >  1:297676/1‑141 (MQ=255)
                                                                                                                   gCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACAAACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGaa                          >  2:783796/1‑141 (MQ=255)
                                                                                                                       aCTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAg                      <  1:542886/141‑1 (MQ=255)
                                                                                                                        atttatttaCGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGAAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGa                     <  2:1054479/140‑1 (MQ=255)
                                                                                                                         tttatttaCGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                    <  2:861212/141‑1 (MQ=255)
                                                                                                                               taCGGGGAGGTATTGTATTTATGAGCGCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGCTTAACGAGTAGGGTATGTTTAGTTGAAGGTGTTTACTTAGAAGTAGATAATaaa              >  2:539926/1‑141 (MQ=255)
                                                                                                                                  ggTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGACGATAAATTTGAAGTTGTTTACTTAGAAGTAGATAATAAAAAt           >  1:352981/1‑141 (MQ=255)
                                                                                                                                  ggTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAt           >  1:948846/1‑141 (MQ=255)
                                                                                                                                       agaaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaaga      >  2:772905/1‑141 (MQ=255)
                                                                                                                                        gaaTTATATTTATCAGCTCATTATGTTAAGCCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGGTAATAAAAATgaagaa     <  1:592095/141‑1 (MQ=255)
                                                                                                                                        gaaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaagaa     <  1:341304/141‑1 (MQ=255)
                                                                                                                                         aaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGGTAATAAAAATGaagaag    >  1:439467/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCA  >  USA300TCH1516_ALE/665252‑665531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑