Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	804,673	A→T	27.0%	L135I (TTA→ATA)	dtpT ←	Di‑/tripeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	804,673	0	A	T	27.0%	140.2 / 50.0	81	L135I (TTA→ATA)	dtpT	Di‑/tripeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base T (10/12);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCT  >  USA300TCH1516_ALE/804537‑804805                                                                                                                                         |                                                                                                                                     aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCt                                                                                                                                  >  2:1135100/1‑141 (MQ=255) aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATAGTTGAAATGTTTGGCTTCATGAAACCt                                                                                                                                  >  2:1069945/1‑141 (MQ=255) aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACACATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCt                                                                                                                                  >  2:1070036/1‑141 (MQ=255)      ttGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAAcc                                                                                                                             <  2:948702/141‑1 (MQ=255)        gCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGACGTTTGTAATGTTGGGCTTCCTTATACCCGAAccca                                                                                                                           >  2:1121635/1‑139 (MQ=255)        gCAAAATAATAGGTGATAATAATGCACCCATACTAACCGACATACAGAAAACAACAAAACCTGAAAAAATACATCAATCCTTTTCAGGCTATAAACGACCAAGTAAATTTGAATTGTTTGGCTTCATTATACCTGCACCaa                                                                                                                           >  2:400065/1‑141 (MQ=255)                  aGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAATACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACGTGATGCATTAATGATgaa                                                                                                                 >  2:775045/1‑141 (MQ=255)                   ggTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAAAGTTTGGCTTCATTATACCTGCACCTATAATGATgaag                                                                                                                >  1:901134/1‑141 (MQ=255)                        taataaTGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACAt                                                                                                           >  2:1124627/1‑141 (MQ=255)                           taacGCACTCATATTAACTGACATATAGAAAAAAACAAAACCTGCATCCATACGTCTATCATTTTCAGTATATAAACGGCCAACGATACTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACAttca                                                                                                        <  2:1055489/141‑3 (MQ=255)                            aatGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAt                                                                                                       >  2:1020072/1‑141 (MQ=255)                             atGCACCCATATTAGCTGACGTATAGAAAGTAACAAAACCTGCATCCATACATCTATCGATATCAGGATATAAACGGCCTAAGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGCTGAAGAACGTTGagg                                                                                                      >  2:495473/1‑139 (MQ=255)                                    cATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAAGGGCCAACGATATTTGAAATGTTGAGATTAATTAAACCTGAACCAAAAAAGATAACCAACATTGAagtggttaa                                                                                               >  1:128056/1‑136 (MQ=255)                                       atTAACTGACATATAGTAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTGTGGCTTCATTAAACCGGATCCAATAAATAAAGAGAACCTTGTGGTGAATatgaa                                                                                            >  1:593732/1‑137 (MQ=255)                                       atTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGcc                                                                                            <  1:178133/141‑1 (MQ=255)                                       atTAACTGACAAATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGcc                                                                                            <  1:735321/141‑1 (MQ=255)                                          aaCTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTAACAATATCAGGATATAAAAAGCCACCGAAATTTGAAATGTTTGGCTTCATAAAGCCTGAACCACTAATAAGGAACAACAGGGGTGTGAATGAGCCGAt                                                                                         >  2:824126/1‑141 (MQ=255)                                            cTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTa                                                                                       >  2:113293/1‑141 (MQ=255)                                              gACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGTTATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAAt                                                                                     <  2:816900/141‑1 (MQ=255)                                                    taGAAAATAACAAAACCTGCATTCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGCATAAGCCGATTACTGCAAAt                                                                               <  1:754793/141‑1 (MQ=255)                                                      gAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATgg                                                                             >  2:610603/1‑141 (MQ=255)                                                       acaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  1:1167775/139‑1 (MQ=255)                                                       aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  1:1020072/141‑1 (MQ=255)                                                       aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  2:566351/141‑1 (MQ=255)                                                       aaaaGAACAAAACCTGCAGCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  2:1115595/141‑1 (MQ=255)                                                          aTAACAAAACCTGCATCCATACGTCTATCAGGTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAAGGGTaga                                                                         >  2:547524/1‑139 (MQ=255)                                                            aaCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt                                                                       <  1:673923/141‑1 (MQ=255)                                                            aaCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt                                                                       <  1:353680/141‑1 (MQ=255)                                                             aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATATCTGAACAAATAGTGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCtt                                                                      >  2:1088210/1‑141 (MQ=255)                                                             aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCATCATTAAACCTGAACCAATAATAATGAAGAACATTGATGTCAATAAGCCCATTAATGCAAATAGTAAACta                                                                      >  2:26076/1‑140 (MQ=255)                                                              cAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGACCATTGATGTGAGTAAGCCGATTAATGCAAATGGTAAGGTTa                                                                     >  2:1092386/1‑141 (MQ=255)                                                              cAAAACCTGCATCCATACGTCTATCATTTTCAGGATAGAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAGGTGAATAAACCGATTAATCCAAATGGTAAGCTTa                                                                     >  2:133953/1‑141 (MQ=255)                                                              cAAAACCTGCATCCATACGTCTATCATTTTCAAGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATCAGCCGATTAATGCAAATGATAAGCTTa                                                                     >  2:1144414/1‑141 (MQ=255)                                                                aaaCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATAATTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAACTGGTAAGCTTaaa                                                                   <  2:861339/141‑1 (MQ=255)                                                                 aaCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAAc                                                                  <  1:1091371/141‑1 (MQ=255)                                                                    cTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTCATGCAAATGTTAAGCATAAACaaa                                                               >  2:958477/1‑141 (MQ=255)                                                                     tGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAGGAACATTGATGTGAATAAGCGGATTAATGCAAATGGTAAGCTTAAGCaaag                                                              >  2:711272/1‑140 (MQ=255)                                                                         tCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAAAGTTTGGCTTCATTAAACCTGAACCAAAAATAAATAAGACCATTGATGTGAAAAAGCAGACTAAAGCAAATAGTAAGCCTAAACaacttgat                                                          >  1:198217/1‑135 (MQ=255)                                                                          ccATACGTGTAGCATTTTCCGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGTTGTGAAGAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTc                                                         <  2:776729/141‑1 (MQ=255)                                                                          ccATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTc                                                         >  2:1068515/1‑141 (MQ=255)                                                                             tACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTccca                                                      >  2:224973/1‑139 (MQ=255)                                                                              ccGTCTATCATTTTCAGGATATAAACGGCCAACGATATTGGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGat                                                     <  1:133413/140‑1 (MQ=255)                                                                                gTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataa                                                   <  1:1135100/141‑1 (MQ=255)                                                                                gTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataa                                                   <  1:672703/141‑1 (MQ=255)                                                                                       aTTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATaaa                                            <  1:420353/141‑1 (MQ=255)                                                                                           tCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACt                                        <  1:216475/141‑1 (MQ=255)                                                                                              ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCa                                     <  2:987429/141‑1 (MQ=255)                                                                                              ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGcc                                     >  2:713224/1‑140 (MQ=255)                                                                                              ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAAAATTGATGTGAATAAGCCGATTAATGCAAATGGTAAACTTAAACAAAAATGTCCGATCATAATAAAGTCTGCa                                     >  2:463839/1‑141 (MQ=255)                                                                                                      aCGGCCACCGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGCGAATAAGCCCATTCCGGCAAAGTTTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCtaataa                             <  1:279746/141‑1 (MQ=255)                                                                                                      aCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCGATAAAGATGAAGAACATTGATGTGAATAAGCCGAATAAAGCAAATGGTAAGCTAAAACCAACATGTCCGAAAATAATAAAGACTGCACCtaataa                             >  1:720630/1‑141 (MQ=255)                                                                                                      aCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCtaataa                             <  1:476297/141‑1 (MQ=255)                                                                                                        ggCCAACGGTATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:649509/141‑1 (MQ=255)                                                                                                        ggCCAACGATCTTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:453924/141‑1 (MQ=255)                                                                                                        ggCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:678520/141‑1 (MQ=255)                                                                                                        ggCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:1177163/141‑1 (MQ=255)                                                                                                        ggCCAACGATATTGGAAATTTTTGGCTACATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:159007/141‑1 (MQ=255)                                                                                                        ggCAAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:630393/141‑1 (MQ=255)                                                                                                         gCCAACGATATTTGACATGTTTGGCTTCATTAAACCTGAACAAATAATGATGAAGAACATTGATGTGAATAAACCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTCCCCCTCATaaaat                          >  2:446216/1‑139 (MQ=255)                                                                                                         gCCAACGATATTTGAAATGTTTGGCTTCCTTCAACCGGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGATAAGATTAAACAAATATGTCAGATAATACTAAAGACTGCACATAATAAAga                          >  2:788386/1‑140 (MQ=255)                                                                                                          ccAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTa                         <  2:560284/141‑1 (MQ=255)                                                                                                              cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAgcgc                     >  1:488203/1‑141 (MQ=255)                                                                                                              cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGAACCTAATAAAGTAgcgc                     >  1:548178/1‑141 (MQ=255)                                                                                                              cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGACAATAATAAAGACTGCACCTAATAACGTAccgc                     >  2:1143778/1‑141 (MQ=255)                                                                                                               gATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGAACCTACAAAAGTAGCGcc                    >  2:84626/1‑141 (MQ=255)                                                                                                                atatTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCct                   >  2:107647/1‑141 (MQ=255)                                                                                                                  ttttGAAAAGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  1:447715/140‑1 (MQ=255)                                                                                                                  atTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  1:1080417/141‑1 (MQ=255)                                                                                                                  atTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  2:1006315/141‑1 (MQ=255)                                                                                                                   tttGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGAATGTACCTAATAAAGTAGCGCATCTa                >  1:449778/1‑141 (MQ=255)                                                                                                                   tttGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTGAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTa                >  1:396702/1‑141 (MQ=255)                                                                                                                     tGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTCATAAAGTAGCGACTCTAGt              >  2:1126424/1‑141 (MQ=255)                                                                                                                      gAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCCAGTg             <  2:828398/141‑1 (MQ=255)                                                                                                                           gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTTTAGTGCCTGt        <  1:867113/141‑1 (MQ=255)                                                                                                                           gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGt        <  1:623342/141‑1 (MQ=255)                                                                                                                           gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGt        <  1:1148568/141‑1 (MQ=255)                                                                                                                            tttGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTa       >  1:123793/1‑141 (MQ=255)                                                                                                                            tttGGCTTCATTAAACATGAACCAATAATGATGAAGAACATTGATGGGAATAAGCCGATTAATGCAAATGGTAAGGTTGGGGAGTTGTGTACGATAATAAGAAAGACTGCACCTAATAAAGTGGCGCCTATAGTGCGTGTa       >  1:591759/1‑141 (MQ=255)                                                                                                                               ggCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAAtt    >  1:971216/1‑141 (MQ=255)                                                                                                                                gCTTCATTAAACCTGAACCAAAAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTc   <  1:113293/141‑1 (MQ=255)                                                                                                                                 cTTTATGAAGCCTGGACCAACAATGATGAACAACATTGATGGGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGTTAATAATAAAGACGGCACCTAATAAAGTAGCGCCTGTAGTGCCTGTAATTCt  <  2:263578/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCT  >  USA300TCH1516_ALE/804537‑804805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑