Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 864,745 C→G 6.6% L21V (CTA→GTA)  est_2 → Carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE864,7450CG 6.6% 286.5 / 10.4 94L21V (CTA→GTA) est_2Carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (37/49);  new base G (4/2);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

AATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  USA300TCH1516_ALE/864606‑864885
                                                                                                                                            |                                                                                                                                            
aaTTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATtact                                                                                                                                             <  2:101458/141‑1 (MQ=255)
aaTTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATtact                                                                                                                                             <  1:177414/141‑1 (MQ=255)
    aaGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACAt                                                                                                                                         <  1:1131353/141‑1 (MQ=255)
      gAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATgg                                                                                                                                       <  1:559067/141‑1 (MQ=255)
       aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTCTTACTACATGGt                                                                                                                                      >  1:416050/1‑141 (MQ=255)
       aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATACAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTCCATGGt                                                                                                                                      <  2:751899/141‑1 (MQ=255)
         tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                    <  1:1137047/141‑1 (MQ=255)
         tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                    >  1:375198/1‑141 (MQ=255)
            aaTAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATACTCATCACGAA‑AGCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTAc                                                                                                                                 <  1:1123488/141‑1 (MQ=255)
            aaTAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑TCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTAc                                                                                                                                 <  2:528805/141‑1 (MQ=255)
               aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAgg                                                                                                                              >  1:972764/1‑141 (MQ=255)
                 tttAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCa                                                                                                                            <  1:1038881/141‑1 (MQ=255)
                  ctAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTATTACATGGTTTTACAGGCaa                                                                                                                           <  1:716719/140‑1 (MQ=255)
                   tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTGTCTTTTTTGAGGAAGGTAAACGTGACGTGTTATTACTAAATGGTTTTACAGGAAAt                                                                                                                          >  2:75741/1‑141 (MQ=255)
                     aCAAGAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGATCTTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTc                                                                                                                        <  1:454236/141‑1 (MQ=255)
                      aaaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCg                                                                                                                       <  2:440842/140‑1 (MQ=255)
                       aaTTAGGTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTAGGCAGATAAAATTACCAAA‑CCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                      <  2:186934/141‑1 (MQ=255)
                       aaTAAGCTATGTCACATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATCAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                      <  2:863971/141‑1 (MQ=255)
                             cTATGTAAAATATATAGCCTAGTTAAGTAGGCAAAGGGAGCGTTAGATTTATGCAGTCACCTCAACCGAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATg                                                                                                                <  1:568230/141‑1 (MQ=255)
                              tATGTAAAATATACAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGt                                                                                                               <  2:863034/141‑1 (MQ=255)
                              tATCTAAAATATATAGCCAAGTTAAGTGTGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGtacgcact                                                                                                               <  2:602463/141‑8 (MQ=255)
                               aTGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTGTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGGTTTACAGGCAATTCGTCTGATGtt                                                                                                              <  2:1001126/141‑1 (MQ=255)
                                    aaaTATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATTCAGTTAAAATCACCAAA‑ACCTTTCTTTTTTGAGGAAAGTAAACCTGCGGTGTTATTAGTACATGGTTTCACATGCAACTGGTCTGATGTGCGTCa                                                                                                         >  2:788917/1‑141 (MQ=255)
                                    aaaTATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCa                                                                                                         <  2:278571/141‑1 (MQ=255)
                                           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  <  2:252593/141‑1 (MQ=255)
                                           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  <  2:146169/141‑1 (MQ=255)
                                             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                <  2:747503/141‑1 (MQ=255)
                                              ccTAGTTAAGTATGCAAAGGGAGCGGTAGATTTAGGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTAGTCTGATGTTCGTCAATTAGGTCGa                                                                                               >  1:206727/1‑141 (MQ=255)
                                                tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                             <  1:445006/141‑1 (MQ=255)
                                                 aGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTGTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCGATTCGTCTGAATTTCGTCAATTAGGATGAttt                                                                                            >  1:798839/1‑141 (MQ=255)
                                                  gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTTTTTTTTTTAAAAGTGACGTGTTGTTACTAGATGGTTTTATAGGCAATTAGGCTGGTGTTTGTTAATTTGGTCGTtttt                                                                                           >  2:992248/1‑141 (MQ=255)
                                                   ttAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGAAGTTCGGCAATTAGGTCgcatttc                                                                                          >  2:586902/1‑135 (MQ=255)
                                                    tAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACATTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATAAGGTCGATTTTTa                                                                                         >  1:271929/1‑141 (MQ=255)
                                                     aaGGATGCAAAGCGGGAGTTAGATTTATGCAGATAAAATTACCAAATAACTTT‑TTGTGTGAGGAAGTTAAACGTGCCGTGTTATGACTACATGGTTTTACAGGTAATTCGTCTGATGTTCGTCAAGTAGGTCGTTTTTTAc                                                                                        <  2:361230/141‑1 (MQ=255)
                                                      aGTATTCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       <  1:627025/141‑1 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:65949/1‑141 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:950114/1‑141 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:1112956/1‑141 (MQ=255)
                                                       gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTGTTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGGCGATTTTTACaa                                                                                      >  1:35593/1‑141 (MQ=255)
                                                       gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                                                      >  2:1180271/1‑141 (MQ=255)
                                                        tATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaaa                                                                                     >  1:1044768/1‑141 (MQ=255)
                                                        tATGCAAAGGGAGAGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaaa                                                                                     <  1:25833/141‑1 (MQ=255)
                                                           gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                  <  2:375198/141‑1 (MQ=255)
                                                             aaaGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑TCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaagaaa                                                                                <  2:173246/141‑1 (MQ=255)
                                                              aaGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaagaaag                                                                               >  1:113988/1‑141 (MQ=255)
                                                               aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                                              <  2:873997/141‑1 (MQ=255)
                                                                  gAGCGTTAGATTTATGCAGATAAAATTACCAAA‑CCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTta                                                                           <  1:1111899/141‑1 (MQ=255)
                                                                    gCGTTAGATTTATGCATATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         <  2:933015/141‑1 (MQ=255)
                                                                    gCGTTAGATTTATGCAGATAGAATTACCAAA‑ACCTTTCTTTTTTCAGGAAGGAAAACGCGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGTTCGATTTTTACAAAAGAAAGGTtata                                                                         >  1:729660/1‑141 (MQ=255)
                                                                    gCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         >  1:836618/1‑141 (MQ=255)
                                                                      gTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTTGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                                       >  1:947707/1‑141 (MQ=255)
                                                                       ttAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTTTTTTTTTTTAACGTGCCGGGTTATTACTACATGGTTTTACAGGCAATTCGTTTGATATTCGTCGATTAGGTCGATTTTTACAAAAGAAAGATtaaaaat                                                                      >  2:245787/1‑136 (MQ=255)
                                                                            tttATGCAGATAAAATTACCAAA‑TCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCatat                                                                 <  2:852326/141‑1 (MQ=255)
                                                                             ttATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTATAAAAGAAAGGTTATACATCATATg                                                                <  1:765048/141‑1 (MQ=255)
                                                                                  cAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                           >  1:709525/1‑141 (MQ=255)
                                                                                   aGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:743413/141‑1 (MQ=255)
                                                                                   aGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:178287/141‑1 (MQ=255)
                                                                                   aGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGCGTTATTACTACATGGGTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:273607/141‑1 (MQ=255)
                                                                                    gATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAACCGTACCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCa                                                         <  2:957046/141‑1 (MQ=255)
                                                                                         aaTTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATg                                                    >  1:416897/1‑141 (MQ=255)
                                                                                         aaTTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATg                                                    >  1:217460/1‑141 (MQ=255)
                                                                                            tACCAAA‑ACCTTTCTTTTTTTAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATACACCGCAATATGAAg                                                 >  1:748966/1‑141 (MQ=255)
                                                                                            tACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAg                                                 >  1:830751/1‑141 (MQ=255)
                                                                                             aCCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAgg                                                <  2:790159/141‑1 (MQ=255)
                                                                                               caca‑aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGTCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:800757/138‑1 (MQ=255)
                                                                                               cAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              >  1:1018656/1‑141 (MQ=255)
                                                                                               cAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              >  1:912066/1‑141 (MQ=255)
                                                                                               cAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:1044768/141‑1 (MQ=255)
                                                                                               cAAA‑ACCTTGCTTTTTTTAGGAAGGTAAACGTGCTGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:206727/141‑1 (MQ=255)
                                                                                                 aa‑aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                            >  1:582683/1‑141 (MQ=255)
                                                                                                  a‑aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGACATATTTCTTATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                           <  1:871187/141‑1 (MQ=255)
                                                                                                   atccTTTCTTTTGTGAGGAAGGTAAAGGTGCCGTGTTATTATGACAGTGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCAGCTGCACCGCAATATGAAGGCCAcg                                           <  2:416050/139‑1 (MQ=255)
                                                                                                   agctttttttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                           <  2:710896/137‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGGGGAAGGTAAACGTGCCGTGTTATTACCACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:731365/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGAACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:927734/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          >  1:481432/1‑141 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:731339/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:920584/141‑1 (MQ=255)
                                                                                                       tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGCAAGGTTATGCATCGTATGCAGCGCAATGTGTAGGCCACGAGGc                                       >  2:936208/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     >  2:1174458/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     >  1:945295/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCAACGCAATATGAAGGCCACGCGGcac                                     >  2:770807/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGACTCATATACATAGAAGGTTCAAGATCATATACACCGCAATATGAAGGCCACGCGGcac                                     >  1:562491/1‑141 (MQ=255)
                                                                                                           ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                   >  1:785324/1‑141 (MQ=255)
                                                                                                           ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                   >  2:877390/1‑141 (MQ=255)
                                                                                                                tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                              <  1:258177/141‑1 (MQ=255)
                                                                                                                 ggcgtgggTAAACGTGCCGTGTTATTACTACTTGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGACACGCGGCACCACCAGAt                             <  1:236313/135‑1 (MQ=255)
                                                                                                                    gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTTGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGTAATATGAAGGCCAGGCGGCACCAACAGATGaa                          >  2:20918/1‑141 (MQ=255)
                                                                                                                     aaGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaaa                         <  1:988960/141‑1 (MQ=255)
                                                                                                                         tAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCCCCAGATGAAATACt                     >  2:1137212/1‑141 (MQ=255)
                                                                                                                           aaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCCTATGCACCGCAATATTAAGGCCACGCGGCACCACCAGATGAAATACTGa                   <  2:901165/141‑1 (MQ=255)
                                                                                                                             cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                 >  2:119607/1‑141 (MQ=255)
                                                                                                                             cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGACCACGCGGCAACACAAGATGAAAAACTGaaa                 >  1:911753/1‑141 (MQ=255)
                                                                                                                               tGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATCCATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATc               <  1:717166/141‑1 (MQ=255)
                                                                                                                                   tggtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCAACGCGTCACCACCAGATGAAATACTGAAATCTAGt           <  1:284564/139‑1 (MQ=255)
                                                                                                                                        tttaCTACATGGTTTTACAGGCAATTCGTGTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCttt      <  2:1154181/140‑1 (MQ=255)
                                                                                                                                            ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  >  2:252673/1‑141 (MQ=255)
                                                                                                                                            ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATCAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  <  2:881562/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAA‑ACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  USA300TCH1516_ALE/864606‑864885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑