Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,577,317 A→T 9.4% V67D (GTT→GAT)  USA300HOU_RS07705 ← Glycyl‑glycine endopeptidase ALE‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,577,3170AT 9.4% 270.1 / 4.2 104V67D (GTT→GAT) USA300HOU_RS07705Glycyl‑glycine endopeptidase ALE‑1
Reads supporting (aligned to +/- strand):  ref base A (39/53);  new base T (9/2);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 2.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GAGCTAATTTTTCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAAACCTTTCATGCATTCT  >  USA300TCH1516_ALE/1577177‑1577457
                                                                                                                                            |                                                                                                                                             
gagCTAATTTTTCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTGTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGaa                                                                                                                                               <  2:1175362/141‑1 (MQ=255)
gagCTAATTTTTATTTTTTATTGGCTTCCACTAACATTACATAAGCTTTCTCAACATCTTTTACACTGGTTTTAGCTTGTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGaa                                                                                                                                               >  2:1134504/1‑141 (MQ=255)
        ttttCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGc                                                                                                                                       >  1:119263/1‑141 (MQ=255)
         tttCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTTAACTTTAAGcc                                                                                                                                      >  2:559808/1‑141 (MQ=255)
         tttCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGcc                                                                                                                                      >  1:837273/1‑141 (MQ=255)
          ttCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCt                                                                                                                                     <  1:105959/141‑1 (MQ=255)
           tCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTa                                                                                                                                    <  1:961037/141‑1 (MQ=255)
           tCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTa                                                                                                                                    <  1:921806/141‑1 (MQ=255)
            cTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAGTACATCTTTTTTTGAACTTTAAGCCTAt                                                                                                                                   >  1:674010/1‑141 (MQ=255)
            cTTTTTTATTGGCTTCTACAAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTAt                                                                                                                                   >  1:715401/1‑141 (MQ=255)
              tttttATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCa                                                                                                                                 <  2:803890/141‑1 (MQ=255)
                      ggCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAAccc                                                                                                                         >  2:524110/1‑141 (MQ=255)
                        cTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGGGAATACATATTTTTTTGAACTTTAAGCCTATCATTTAACCCCt                                                                                                                       >  2:322187/1‑141 (MQ=255)
                         ttCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACAGCTGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCtt                                                                                                                      <  1:326581/141‑1 (MQ=255)
                         ttCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCtt                                                                                                                      <  1:925097/141‑1 (MQ=255)
                         ttCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCtt                                                                                                                      <  1:524110/141‑1 (MQ=255)
                            tactaACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAAt                                                                                                                   >  1:511608/1‑141 (MQ=255)
                              ctaACTTTAAATATGCTTTCTCATCATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATtc                                                                                                                 <  2:264808/141‑1 (MQ=255)
                                aaCTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATtctc                                                                                                               <  1:814225/141‑1 (MQ=255)
                                        atatGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACGCCTTAATTCTCGCCTGata                                                                                                       >  1:775955/1‑141 (MQ=255)
                                             cTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTATCCCCTTAATTCTCTCTTGatatcctc                                                                                                  >  2:1196299/1‑137 (MQ=255)
                                                tctcAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCa                                                                                              <  2:963697/141‑1 (MQ=255)
                                                 ctctACGTCTTTTACCCTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTGAAGCCTAGCTTTTAACCCCTTAATTCTCGCCTGATATTTTTCCAt                                                                                             <  2:358818/141‑1 (MQ=255)
                                                   cAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTTATTTTTATGCCTATCATTTACCCCCTTAATTCTTCGCTGATATTTTTCCCTTg                                                                                           >  2:86152/1‑141 (MQ=255)
                                                    aaCATCTTTTACTCTGTATTTAGCTTTTTGGTAATTATCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGa                                                                                          <  2:811311/141‑1 (MQ=255)
                                                    aaCATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTATGCTCATGTTCTACTTGAGACTACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGa                                                                                          >  2:421714/1‑141 (MQ=255)
                                                    aaCATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGa                                                                                          <  1:609216/141‑1 (MQ=255)
                                                    aaCATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATATAACCCCTTAATTCTCGCCTGATATTTTTCCATTGa                                                                                          <  1:829339/141‑1 (MQ=255)
                                                     aCATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGAt                                                                                         >  1:853164/1‑141 (MQ=255)
                                                      cATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGCACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGAtt                                                                                        >  2:137191/1‑141 (MQ=255)
                                                      cATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGCtt                                                                                        >  2:188568/1‑141 (MQ=255)
                                                        tCTTTTACACTGGATTGAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGAtttt                                                                                      <  1:393657/141‑1 (MQ=255)
                                                         cTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTCTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGAttttt                                                                                     <  2:1027266/141‑1 (MQ=255)
                                                          ttttACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGAtttttt                                                                                    >  2:942714/1‑140 (MQ=255)
                                                          ttttACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTc                                                                                    >  1:988365/1‑141 (MQ=255)
                                                              acacTGGATTTAGCTTTTTTTTTTTTTTTTTTACCTTGTTTAGGCTCATCTTCTACTTGAGAGTACTTGTTGTTTTGAACTTTATGCCTCTCATTTAACCCCTTTATTCTCGCCTGAGATTTTTCTGTTTTTTTTTTAGAc                                                                                >  2:130668/1‑141 (MQ=255)
                                                               cacTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACt                                                                               <  1:1079531/141‑1 (MQ=255)
                                                                acTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACtt                                                                              <  1:1074741/141‑1 (MQ=255)
                                                                  cggATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTAt                                                                            <  1:915476/140‑1 (MQ=255)
                                                                       ttAGCTTTTTGGTAATTAGCGTTAACTTGTCTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAAt                                                                       <  1:161925/141‑1 (MQ=255)
                                                                          gCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCt                                                                    <  2:119263/141‑1 (MQ=255)
                                                                          gCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACCTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTGCATTGATTTTTCAGCCTTATCAAATGCt                                                                    >  1:678600/1‑141 (MQ=255)
                                                                           cTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTg                                                                   >  1:1105356/1‑141 (MQ=255)
                                                                            tttttGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGa                                                                  <  2:100915/141‑1 (MQ=255)
                                                                             ttttGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTTTTTTTTTTTCCTATCATTTAACCCCTTAATTTTCGCCTGATATTTTTCCATTGATTTTTCTGACTTTTCAAATGCTGat                                                                 >  2:877238/1‑140 (MQ=255)
                                                                                tGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGa                                                              <  1:22914/141‑1 (MQ=255)
                                                                                tGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGa                                                              <  1:292218/141‑1 (MQ=255)
                                                                                tGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGa                                                              >  1:1028375/1‑141 (MQ=255)
                                                                                    aaTTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCGTTGATTTTTCAGACTTATCAAATGCTGACAGATTAg                                                          >  1:326284/1‑141 (MQ=255)
                                                                                      ttAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAAGGCTGACAGATTAGCt                                                        <  1:598652/141‑1 (MQ=255)
                                                                                       tAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACGTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTCTCAAATGCTGACAGATTAGCtt                                                       <  2:874890/141‑1 (MQ=255)
                                                                                        aGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACACCTTTTTTTGAACTTTAAGCCTATCATTTACCCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCttt                                                      <  2:1171/141‑1 (MQ=255)
                                                                                           gTTAACTTGGTTAAGCTCATCTCCTTCGTGAGAATACCTCTTTTTTTGAACTTTAAGCAGTTCCGGGTACTCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcat                                                   <  1:275564/141‑1 (MQ=255)
                                                                                            ttAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTATCTCGCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcatt                                                  <  1:1059858/141‑1 (MQ=255)
                                                                                            ttAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCGATCATTTAACCCCTTAATTCGCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcatt                                                  <  1:33404/141‑1 (MQ=255)
                                                                                              aaCTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcatttc                                                <  1:390558/141‑1 (MQ=255)
                                                                                              aaCTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcatttc                                                <  1:964054/141‑1 (MQ=255)
                                                                                              aaCTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCtttcatttc                                                <  1:467432/141‑1 (MQ=255)
                                                                                                  ttgttATGCTCATCCGCCGCTTGATAACACATCTGTTTTTGATCTTTAAGCCTATCATTTAACCCCTTAATCCTCGCCTGATATTTTCCCATTGATTTTTCAGACTTATCAAATGCTGACCGATTAGCTTTCATTTCACTa                                            <  2:522905/138‑1 (MQ=255)
                                                                                                     ttAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTa                                         >  1:1202253/1‑141 (MQ=255)
                                                                                                       aaGCTCATCTTCTACTTGAGAATACATCTTTTTTTTTTTTTTTTTCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCGATTGATTTTTCAGACTTTTCAAATGCTGACAGATTTACTTTCATTTCTCTTTTaac                                       >  2:714433/1‑141 (MQ=255)
                                                                                                       aaGCTCATCTTCTACTTGAGAATACATCTTTTTTTTTTTTTTTTTCAAATTGTATAAGCCCTTCATTATCGCCTCTTGTGTTTCTATTTATATTTCATACTTTTCCGATCTTGACCGTTTAGCTTTCATTTCACACTTaac                                       >  2:345438/1‑141 (MQ=255)
                                                                                                       aaGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGTCCATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTaac                                       <  2:781787/141‑1 (MQ=255)
                                                                                                       aaGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTGTTaaa                                       >  2:943154/1‑140 (MQ=255)
                                                                                                           tCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTTAGCCTATCATTTACCCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCCGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAacaaca                                   <  2:786579/141‑1 (MQ=255)
                                                                                                            cATCTTCTACTTGCGACAACATCTTCTTTAAATCTATAACCATATCCAGTATGCCCATGAGTCTCCCCTAATATTGTTCCTTTTAGTTTTCATACTTATCAAATGCTGTCATATTAGCTTTCATTTCACTATTAACAacac                                  <  2:399153/141‑1 (MQ=255)
                                                                                                             aTCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACAcc                                 >  2:243473/1‑141 (MQ=255)
                                                                                                              tcttctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTCGCTTTCCTTTCACCATTAACAACACCt                                >  1:944231/1‑141 (MQ=255)
                                                                                                              tcttctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCt                                >  1:944215/1‑141 (MQ=255)
                                                                                                              tcttctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCt                                >  1:946982/1‑141 (MQ=255)
                                                                                                                 tctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCATACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAAt                             >  2:579235/1‑141 (MQ=255)
                                                                                                                 tctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAAt                             >  1:1115150/1‑141 (MQ=255)
                                                                                                                 tctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAAt                             <  1:1008470/141‑1 (MQ=255)
                                                                                                                 tctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAAt                             <  1:501323/141‑1 (MQ=255)
                                                                                                                 tctACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAAt                             <  1:631096/141‑1 (MQ=255)
                                                                                                                  ctACTTGAGAATACATCTTTTTTTTTTTTTTTTTCCTATCATTTAACCCCTTAATTTTCGCCTGATATTTTTCCATTGATTTTTCTGACTTATCAAATGCTGACTGATTTCTTTTTTTTTCACTATTATCTACTCCTTAtt                            >  2:330859/1‑141 (MQ=255)
                                                                                                                   tACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGCCTTATCAAATGCTGACAGATTAGCTATCATTTCGCTATTAACAACACCTAATTg                           <  1:775610/141‑1 (MQ=255)
                                                                                                                   tACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTCACAACACCTAAtgg                           >  2:273649/1‑139 (MQ=255)
                                                                                                                     cTTGAGAATACATCTTTTTTTGATTTTTTTGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTTTCAAATGCTTTCAGGTTAGCTTTCCGTTTTTTATTAACAACGCCTAtttttt                         >  1:157515/1‑138 (MQ=255)
                                                                                                                      ttGAGAATACATATGTTCTTGAACTTTAAGACTAGCAGCTAACCTCTTCAATCGAGCCCGATCTACTGCCAAGGATTTTTCGGGCTCATCAAATGCTAATGAAGTAGCTTTCATTTCACTATTAACAACACATACTTGtag                        >  2:587809/1‑139 (MQ=255)
                                                                                                                         agaATACATCTTTTTTTTTTTTTTTAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTTACTACACTTAATTGTCGCtt                     >  2:827762/1‑141 (MQ=255)
                                                                                                                         agaATACATCTTTTTTTGAACTTTTAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCCTTTCACTATTAACAACTCCTAATTGTCGCtt                     <  2:337306/141‑1 (MQ=255)
                                                                                                                         agaATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCtt                     <  1:559808/141‑1 (MQ=255)
                                                                                                                          gaATACATTTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCttt                    <  2:305927/141‑1 (MQ=255)
                                                                                                                          gaATACATCTTTTTTTTTTTTTTTTTCTTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGTTTTTTCAGACTTATCAAATGCTGCCAGTTTAGCTTTCATTTCACTATTATCTTCACCTTATTTTCTCttt                    >  1:496051/1‑141 (MQ=255)
                                                                                                                          gaATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCttt                    <  1:648087/141‑1 (MQ=255)
                                                                                                                            aTACATCTTTTTTTTAACTTTATTCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACCGATTAGCTTTCATTTCCCTATTAACACTACCTAATTGTCTCTTTaa                  >  1:1015603/1‑141 (MQ=255)
                                                                                                                            aTACATCTTTTTTTGAGTTTTAAGGCTATCATTTAACCCCTCAATTCACGGCTGATATTTTTCCATTGATTTTTCAGACTTATAAAATTCTGACAGATCAGGTTTAATTTCACTATTAAACACAACTAATTGTGGAtttta                  >  2:388859/1‑139 (MQ=255)
                                                                                                                            aTACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTaa                  <  1:421714/141‑1 (MQ=255)
                                                                                                                            aTACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATCCTGGCCTGATATTTTTCGATTGATTTTTCAGACTCATCAACTGTTGACAGATCAGCGTTCATTTCACTATTACCACCATCTAATTGTCGCTTTaa                  >  2:877125/1‑141 (MQ=255)
                                                                                                                              aCATCTTTTTTTGAACTTTAAGCCTATCATTTGACCCCTTAATTCTCGCCTGATGTTTTTCCATTGATTTTTAAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTCTTAACAACACCTTATTGTCGCTTTaaaa                >  1:440424/1‑140 (MQ=255)
                                                                                                                                    ttttttGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTTGCTTTAAACCTTTCa          <  1:1134504/141‑1 (MQ=255)
                                                                                                                                    ttttttGAACTTTAAGCCTATCAGTTAACCCCTTAATTCTCGCCTGATATTTTTCCATCGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAACCCTTTCa          <  1:754172/141‑1 (MQ=255)
                                                                                                                                      ttttGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTTTTTCTTTTTAAATGCTGACAGATTATTTTTCATTTCACTATTTATAACACCTAATTATAGCTTTAATCTTTTCAtt        >  2:795647/1‑140 (MQ=255)
                                                                                                                                      ttttGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAAACCTTTCagg        >  1:490976/1‑139 (MQ=255)
                                                                                                                                       tttGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGCTCTTTTATGCCATAGTGTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAAACCTTTCATGc       <  1:518649/141‑1 (MQ=255)
                                                                                                                                       tttGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAAACCTTTCATGc       <  2:674010/141‑1 (MQ=255)
                                                                                                                                        ttGAACTTTAAGCCTATCATTTAAACCCTTAATTCTAGACTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTCACAGCTTAGCTTTCCTTTCACTATTCACAACACCTACTTTTCGCTTTAAACCTTTCCTgac      >  2:44127/1‑139 (MQ=255)
                                                                                                                                         tGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGCTTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCCCTCTTCACAACACCTAATTGTAGCTTTAAACCTTTCATGCAt     >  2:192204/1‑141 (MQ=255)
                                                                                                                                            aCTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTTTCGCTTTAATCCTTTCATGCATTCt  <  1:600174/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
GAGCTAATTTTTCTTTTTTATTGGCTTCTACTAACTTTAAATATGCTTTCTCAACATCTTTTACACTGGATTTAGCTTTTTGGTAATTAGCGTTAACTTGTTTAAGCTCATCTTCTACTTGAGAATACATCTTTTTTTGAACTTTAAGCCTATCATTTAACCCCTTAATTCTCGCCTGATATTTTTCCATTGATTTTTCAGACTTATCAAATGCTGACAGATTAGCTTTCATTTCACTATTAACAACACCTAATTGTCGCTTTAAACCTTTCATGCATTCT  >  USA300TCH1516_ALE/1577177‑1577457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: