BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	100%	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	325.8 / NA	95	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (56/39); total (56/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  USA300TCH1516_ALE/1646053‑1646324

tttATCACTTGCAACACCATGTGGTAATGGACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGACTTTATTTCTTTTTTTGTCATGCCTCCttt                                                                                                                                      >  1:321427/1‑141 (MQ=255)

 ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATACCTCCtttt                                                                                                                                     >  2:761007/1‑141 (MQ=255)

 ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAAACGTATCGAATGATGTTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                     >  2:529219/1‑141 (MQ=255)

  tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑a                                                                                                                                   >  2:271714/1‑141 (MQ=255)

   ttCACTTGCAGTACCGTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ac                                                                                                                                  <  2:988688/140‑1 (MQ=255)

   aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ac                                                                                                                                  >  1:357874/1‑141 (MQ=255)

   aTCACTTGCAACACCATGTGGTAATACACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ac                                                                                                                                  >  2:1060083/1‑141 (MQ=255)

    tCACTTGCAACACCATGTGGTAATGGACCCCTATGACCAGATGCTACAATCGTATCGGATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTACCTTTAATTGTTTTTCAGTCATGCCTCCTTTT‑act                                                                                                                                 >  2:683928/1‑140 (MQ=255)

       cTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑acaaca                                                                                                                              >  2:1059102/1‑141 (MQ=255)

        ttGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAg                                                                                                                             <  1:364272/141‑1 (MQ=255)

            aaCACCATGTGGTAATGCACCTCTAAAACAAGATGCTACAATCGTATCTAATGCTGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTACCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTaa                                                                                                                         >  2:529217/1‑141 (MQ=255)

              ctccATGTGGTAATGCACCTCTATGGCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTaaaa                                                                                                                       <  1:935132/139‑1 (MQ=255)

              cacCTTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTaaaa                                                                                                                       <  2:649263/141‑1 (MQ=255)

              cacCATGTGGTAATGCACCTCTATGCCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTaaaa                                                                                                                       <  2:649676/141‑1 (MQ=255)

                   tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAatatat                                                                                                                  >  2:945944/1‑141 (MQ=255)

                    gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATAtt                                                                                                                 <  2:275815/141‑1 (MQ=255)

                     tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTc                                                                                                                >  1:256018/1‑141 (MQ=255)

                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCa                                                                                                               <  1:415708/141‑1 (MQ=255)

                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCa                                                                                                               >  1:271963/1‑141 (MQ=255)

                       gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCat                                                                                                              <  1:300346/141‑1 (MQ=255)

                           tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGt                                                                                                          <  1:758954/141‑1 (MQ=255)

                             cACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGttt                                                                                                        <  1:252245/141‑1 (MQ=255)

                              aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTtc                                                                                                       <  1:662523/141‑1 (MQ=255)

                                ctctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTtctt                                                                                                     >  1:485574/1‑139 (MQ=255)

                                           gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTTATTCTTTTTCAGTCATGCATCCTTTT‑GCAACAGTTAAAATATATTCATATGTTTTATCAACAATATTa                                                                                          >  2:395865/1‑141 (MQ=255)

                                             tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                        >  2:34955/1‑141 (MQ=255)

                                               cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATCTTAgctg                                                                                      >  2:266555/1‑141 (MQ=255)

                                               cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                      <  2:1016373/141‑1 (MQ=255)

                                                tACAAGCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTCACCCCA‑TTGAATGAGCTTCATATGTTTCATCAACAATATTAgctgc                                                                                     >  2:539947/1‑141 (MQ=255)

                                                 aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                    >  2:24488/1‑141 (MQ=255)

                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCCGTCATGCCCCCTTTT‑ACAACAGTTAAAATATATTCGTATGTTTCTTCAACAATATTAGCTGCtt                                                                                   <  1:780457/141‑1 (MQ=255)

                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                   >  1:288132/1‑141 (MQ=255)

                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                   <  1:700215/141‑1 (MQ=255)

                                                    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                 <  1:1019574/141‑1 (MQ=255)

                                                    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                 <  1:673328/141‑1 (MQ=255)

                                                      cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCCACAATATTAGCTGCTTTTTg                                                                               >  2:1184710/1‑141 (MQ=255)

                                                         aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                            >  1:124721/1‑141 (MQ=255)

                                                         aTCGAATGATGGTCCATCTACTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAGTTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATCTGTTTCATCAACACTATTAGCTGCTTTTTGAAt                                                                            >  2:667000/1‑141 (MQ=255)

                                                          tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                           <  1:837818/141‑1 (MQ=255)

                                                           ctaaTGATGGTCCATCTGCTCCTAATTCTATCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTa                                                                          <  1:230923/139‑1 (MQ=255)

                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                       >  1:670356/1‑141 (MQ=255)

                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                       >  2:276445/1‑141 (MQ=255)

                                                               tgatgGTCCATCTGCTCCTAATTCTTGCATTTTGCTTTCTAATATTTCCTTTAATTCTGTTGCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATCGTTTATCAACAATATTAGCTGCTTTTTGAATTAAAGc                                                                      <  2:787395/141‑1 (MQ=255)

                                                               tgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTGTCTAATATTGCCTTTAATTCTTTTTCTGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGc                                                                      <  1:809526/141‑1 (MQ=255)

                                                                gatgGTCCATCTGCTCCTAATTCTAGCTTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTGTT‑AGAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                     <  2:682649/141‑1 (MQ=255)

                                                                 atgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGACTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATGACCAATGTTAGCTGCTTTTTGAATTAAGGCaa                                                                    >  1:398669/1‑141 (MQ=255)

                                                                 atgGTCCATCTGCTCCTAATTCTAGCATTTTGATTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                    >  1:856913/1‑141 (MQ=255)

                                                                  tgttCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAACATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                                   <  2:933003/138‑1 (MQ=255)

                                                                    gTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt                                                                 >  2:99065/1‑141 (MQ=255)

                                                                        ttCTGCTCCTAATTATAGCCTTTTGCCTTATAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                             <  1:539947/140‑1 (MQ=255)

                                                                         tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCa                                                            >  2:593160/1‑141 (MQ=255)

                                                                           tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCACTTTCGTCCGc                                                          >  1:570904/1‑141 (MQ=255)

                                                                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCCTCAGCAt                                                        <  1:751633/141‑1 (MQ=255)

                                                                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTCTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCGGCAt                                                        >  1:828786/1‑141 (MQ=255)

                                                                              tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATc                                                       >  1:452947/1‑141 (MQ=255)

                                                                                cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCatgatcgt                                                     >  2:737220/1‑134 (MQ=255)

                                                                                 tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                    <  2:356205/141‑1 (MQ=255)

                                                                                 tAATTCTAGCATTTTGCTTTCTAATATTGCCTTCAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAACttt                                                    <  1:812690/141‑1 (MQ=255)

                                                                                      cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                               <  1:402043/141‑1 (MQ=255)

                                                                                         gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTACAGCAATTTCGTCAGCATCTTTGACGtctc                                            >  1:730845/1‑141 (MQ=255)

                                                                                             tttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                        <  1:24488/141‑1 (MQ=255)

                                                                                              ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACtt                                       >  2:1123368/1‑141 (MQ=255)

                                                                                              ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                       <  1:1060083/141‑1 (MQ=255)

                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCGCTAATTTTAt                                   >  2:59438/1‑141 (MQ=255)

                                                                                                    tCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                 <  2:324197/141‑1 (MQ=255)

                                                                                                      tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCCTATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTaa                               >  2:197415/1‑140 (MQ=255)

                                                                                                       aaTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTAcc                              >  2:5358/1‑140 (MQ=255)

                                                                                                       aaTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTACTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCAAATTTTATCTACa                              >  2:321252/1‑141 (MQ=255)

                                                                                                         tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                            <  1:981883/141‑1 (MQ=255)

                                                                                                          atTGCCTTTAATTCTTTTTCCGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGtt                           >  1:1005630/1‑140 (MQ=255)

                                                                                                          atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTCAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTTGTCAGCATCTTTGGCGACTCTAATTTTATCTACAGTa                           >  1:161789/1‑141 (MQ=255)

                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATTTGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         >  2:8292/1‑141 (MQ=255)

                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         <  2:532289/141‑1 (MQ=255)

                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         >  1:137506/1‑141 (MQ=255)

                                                                                                               cTTTAATTCTTTTTCAGTCATGCCTCCTTTT‑AAACTTCGTGAAAAAAATTCTTTCTTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                      >  1:280898/1‑141 (MQ=255)

                                                                                                                ttttATTCTTTTTCAGTCATGCTTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTATCTACAGTATTAGaa                     <  1:529219/141‑1 (MQ=255)

                                                                                                                tttAATTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                     <  1:761007/141‑1 (MQ=255)

                                                                                                                    aTTCTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                 >  2:501444/1‑141 (MQ=255)

                                                                                                                    aTTCTTTTTCAGTCATGACTCCTTTT‑ACAAAAGTTAAAATATATTCCTCTGTTTCATAAAGAATCTTAGCTGATTTTTGAATTCAAGAATTTTAGTCAGCATAGTTGACGTCTCTAATTTTATCTACCGAATTATAAAtac                 >  2:211619/1‑139 (MQ=255)

                                                                                                                       cTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta              >  1:970746/1‑141 (MQ=255)

                                                                                                                       cTTTTTCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAGGCtta              >  1:970500/1‑141 (MQ=255)

                                                                                                                        tttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat             >  2:885311/1‑141 (MQ=255)

                                                                                                                        tttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGAAGTCTCTAATTTTATCTACAGTATTAGAAATGCttat             >  2:691413/1‑141 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGCCGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt            <  1:34955/141‑1 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt            >  2:765126/1‑141 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTCATCTACAGTATTAGAAATGCttatt            >  1:1052470/1‑141 (MQ=255)

                                                                                                                         ttttCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATT‑AAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta           >  1:597999/1‑141 (MQ=255)

                                                                                                                            tCAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAACGACATTTCGGCATCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt         <  1:1123368/141‑1 (MQ=255)

                                                                                                                             cAGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg        <  1:271714/141‑1 (MQ=255)

                                                                                                                              aGTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa       <  2:729833/141‑1 (MQ=255)

                                                                                                                               gTCATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat      >  2:643461/1‑141 (MQ=255)

                                                                                                                                 cATGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAGTGCTTATTAATGatat    >  1:508191/1‑141 (MQ=255)

                                                                                                                                  aTGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAtata   >  1:1041869/1‑141 (MQ=255)

                                                                                                                                   tGCCTCCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc  >  1:450705/1‑141 (MQ=255)

                                                                                                                                   tGCCTCCTTTT‑ACAACAGTTAAAATATATTCAGTTCTTTCATCCACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGTATCTTTGACGTCTCTTATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc  <  2:265226/141‑1 (MQ=255)

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTT‑ACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  USA300TCH1516_ALE/1646053‑1646324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`