| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,963,768 | C→G | 24.0% | V365L (GTA→CTA) | hemY ← | Protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,963,768 | 0 | C | G | 24.0% | 270.5 / 91.1 | 137 | V365L (GTA→CTA) | hemY | Protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (57/47); new base G (18/15); total (75/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
CATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTTTT > USA300TCH1516_ALE/1963628‑1963907 | cATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAc < 1:970016/141‑1 (MQ=255) aTACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCACTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACa < 1:823700/141‑1 (MQ=255) cTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATAc < 1:1063157/141‑1 (MQ=255) tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGc > 2:1136823/1‑141 (MQ=255) tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGc > 2:143284/1‑141 (MQ=255) tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACATGGCTTACCTACATCCGc > 2:52043/1‑141 (MQ=255) tCGTCAAACCATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCAc < 1:305513/141‑1 (MQ=255) tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACGACAGTATCACCTGGTTTACCTACATACGCAc > 2:640393/1‑141 (MQ=255) tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCCCAGTATCACCCGGTTTACCTCCATACGCAc > 2:471016/1‑141 (MQ=255) tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCAc < 2:712900/141‑1 (MQ=255) tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGCTTACCTAGATACCCAc > 1:596038/1‑141 (MQ=255) tCGGCAAACGAGTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCCCAGAATCACCTGGTTTACCTACATACGCAc > 2:937911/1‑141 (MQ=255) cGGCAAACGATTGACACTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg < 2:1016295/141‑1 (MQ=255) cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATActctcg < 2:1037036/141‑6 (MQ=255) cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg > 2:523643/1‑141 (MQ=255) cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg < 2:1118846/141‑1 (MQ=255) cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg < 1:183022/141‑1 (MQ=255) ggCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGa < 2:895934/141‑1 (MQ=255) ggCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTAAAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGa < 2:576020/141‑1 (MQ=255) cAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAt < 2:1191145/141‑1 (MQ=255) aaaCGATTGACAATGGTAAATTCGGGATCACCTTTAAATGTCATCATTTGACTTAAATCGCTACGTACAATCGATACTAATTCATTATCTGTATGATCATGAGCCACAGTATCACCTGGTTTACCTACCTACCACCGAATc > 2:640848/1‑141 (MQ=255) aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCCGAAATCaa > 2:99301/1‑141 (MQ=255) gATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaaaa > 1:851531/1‑141 (MQ=255) aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAac > 1:302014/1‑141 (MQ=255) aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGACCGAATCAAAac > 2:241371/1‑141 (MQ=255) ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACACCAGTATCACCTGGTTTAACTACATACGCCCGAATCAAAacc > 2:472516/1‑141 (MQ=255) tGACAATTGTAAATTCAGGATCACTTTTAAATGTCATCATTTGACTTAAAGCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAacct < 2:522160/141‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTGAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCAGAGTAGCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct < 1:525365/141‑1 (MQ=255) aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCgg > 1:626791/1‑141 (MQ=255) aaaTTCAGGATCACCTTTAAATGTCAGCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCgg < 2:171079/141‑1 (MQ=255) aTTCAGGATCACCTTAAAATGTCATCATTTGACTTAAATCTCTACGCACAATCGATACTACAACCTTATCTGTATGACCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAACCCTTACCTTCCGgtg < 1:457711/141‑1 (MQ=255) ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGgtgt < 2:483468/141‑1 (MQ=255) tCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAAt > 2:1097797/1‑141 (MQ=255) tCACCTGTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAAt > 2:1066070/1‑141 (MQ=255) ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc < 1:259960/141‑1 (MQ=255) tttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCa < 2:204175/141‑1 (MQ=255) ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGccct > 2:52392/1‑139 (MQ=255) tGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAAACACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCAtttttt > 2:303442/1‑141 (MQ=255) catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa > 1:1163041/1‑141 (MQ=255) catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa > 1:897530/1‑141 (MQ=255) ggcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTAACTACATACGCACTAATCAAAACCTTCCATTCCGGATTAGTAAATGGCCATTTTTTCGAt > 2:190896/3‑141 (MQ=255) tgatTTAACTTAAATCTCTAAGTACACTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg < 2:95289/139‑1 (MQ=255) tcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAtt > 2:413723/1‑140 (MQ=255) gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg < 1:948307/141‑1 (MQ=255) aCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt < 2:986364/141‑1 (MQ=255) aCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt < 1:143284/141‑1 (MQ=255) gttAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGtc > 2:735502/2‑140 (MQ=255) cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTa > 2:179620/1‑141 (MQ=255) cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTCCCGGTGTTGTAAATGGCCATTTTTTCGTTGTCCAAGtc > 2:1001827/1‑140 (MQ=255) cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTa > 1:249577/1‑141 (MQ=255) attAAATCACTACGTACAATAGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCATTTTTTCGATGTCCAAGTa > 2:403817/2‑141 (MQ=255) ttAAATCTCTACGTACACTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAc < 1:153898/141‑1 (MQ=255) ttAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAc < 1:146320/141‑1 (MQ=255) tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 1:1134503/141‑1 (MQ=255) aaaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGACTTCCGAGTAAAt > 2:997075/1‑141 (MQ=255) aatccgtACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATg < 2:156413/135‑1 (MQ=255) tctcTACGTACAATCTATACTAACTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg < 1:411515/141‑1 (MQ=255) tctcTACGTACAATCGATACTAATTCATTAGCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg < 1:472516/141‑1 (MQ=255) tccttgcGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGATTCAAATCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg < 1:410937/135‑1 (MQ=255) tctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACGTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAGTCGGt > 1:36154/1‑141 (MQ=255) tctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCAACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGt < 2:891696/141‑1 (MQ=255) aCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAAt > 1:1037095/1‑141 (MQ=255) aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTTCAAGTGCATGCGGTaaggact > 1:1169669/1‑136 (MQ=255) tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTAt < 1:502303/141‑1 (MQ=255) gATACTCATCCACTACCTGTATTATCATCCACCACAGTCTAACATGGTTTCCCTGCATTCGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTCTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa < 1:161420/141‑1 (MQ=255) gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGGAATGTCTGTATCa > 2:349085/1‑141 (MQ=255) aTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTCCCTACATACGCACGAATCAAAACCTTACCTTCCGGCGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTACCACTCGTtc > 2:1170876/1‑141 (MQ=255) tCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGGTTCACGCGTtctc > 1:447575/1‑141 (MQ=255) ctcaTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGc < 1:555182/138‑1 (MQ=255) cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTa > 1:787171/1‑141 (MQ=255) cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTa > 1:641077/1‑141 (MQ=255) tGTATGATCATCAACCCCAATGTCACCTGGTTTACCTACATACGCACGAATGAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCCATTTCCCAGGACATGCGGTAATGGCGGTATCACTCGTTCTCGcgactag > 2:850595/1‑135 (MQ=255) tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCGCGAATAAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAAGGTCTATGTCACTCGTTCTCGCACTTACGAAg > 1:1127592/1‑141 (MQ=255) tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAg > 1:1127896/1‑141 (MQ=255) tGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCACAATTACGAAg > 1:820781/1‑141 (MQ=255) tGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTGCTCGCAATTACGAAg > 1:339023/1‑141 (MQ=255) tGATCATCAACCAACGTATCACCTGGTTTACCTACATACGCACAAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCGATTACGAAg > 1:235838/1‑141 (MQ=255) gATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTGCTTTCTTTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc > 1:513658/1‑141 (MQ=255) gATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc < 2:1139370/141‑1 (MQ=255) atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAGGTCCAAGTACATGCGGTAATGTCGGTATCACTCGTTCTCGCAATCACGAAGcc > 2:460768/1‑141 (MQ=255) tcatcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcaa > 1:925960/1‑139 (MQ=255) catcaACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg < 1:478324/141‑1 (MQ=255) aCCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATTGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTAGCAATTACGTAGCCAGTAccc > 1:30212/1‑140 (MQ=255) ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCCGTGCcaa > 2:41881/1‑140 (MQ=255) acaGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCCCGCAATTACGAAGCCAGTACcatca > 2:449359/1‑141 (MQ=255) acaGTACCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatca < 2:258718/141‑1 (MQ=255) caGTATCACCTGGTTTACCTGGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat < 2:168432/141‑1 (MQ=255) caGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat > 2:861935/1‑141 (MQ=255) caGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTTTTTTTACATGCAGTAATGTCTGTATCACTCGTTCTCGCAATTGCGAAGCCAGTACcatcat > 2:282046/1‑141 (MQ=255) caGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCCGTATCACTCTTTCTCGCAATTACGAAGCCAGTACcatcat < 1:1066070/141‑1 (MQ=255) gTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCACTCACGAAGCCAGTACCATCATgg > 1:268353/1‑141 (MQ=255) ttCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGt < 2:941867/140‑1 (MQ=255) ccTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAttt > 1:1199567/1‑141 (MQ=255) cTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGACCATTTGTTCGATGTCCAAGTACGTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTCAGAAGCCAGTACCATCATGGGCAgttt > 2:328331/1‑141 (MQ=255) tGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc < 2:36154/141‑1 (MQ=255) cgcttACCTACATACACACCAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGACGTCCAAGTACATGCGGTAATGTCCGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCa < 2:268729/138‑1 (MQ=255) ttACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTCTCACTCGTTCGCGCAATTACGAAGCCAGTACCATCATGGGTCGTTTCaagg > 1:682544/1‑139 (MQ=255) ccTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCt > 1:445929/1‑141 (MQ=255) cTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCtt > 1:303244/1‑141 (MQ=255) aCATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGTTGTCGAAGTGCATGCGCGAATGTCTGTATCACTGGTTCTCGCAATTACGCAGCCAGTACCATCATGGGTATTTTCGCTGTCtttt > 2:960416/1‑141 (MQ=255) | CATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTTTT > USA300TCH1516_ALE/1963628‑1963907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |