BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,028,394	A→C	68.3%	F84C (TTT→TGT)	USA300HOU_RS10230 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,028,394	0	A	C	68.3%	120.1 / 61.8	90	F84C (TTT→TGT)	USA300HOU_RS10230	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (11/17); new base C (29/31); total (40/50)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

ATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAA  >  USA300TCH1516_ALE/2028255‑2028533

atataCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCCCAATAAGTACTaaaaaaaa                                                                                                                                            >  2:834729/1‑141 (MQ=255)

atataCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATaaaaa                                                                                                                                            >  1:640480/1‑141 (MQ=255)

  ataCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGa                                                                                                                                          >  1:415142/1‑141 (MQ=255)

   taCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAGTAACGCACAATAAGTAATAATAAACAGAc                                                                                                                                         >  1:833775/1‑141 (MQ=255)

   taCATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAAGACACAATAAGTAATAATAAACAGAc                                                                                                                                         >  1:1085196/1‑141 (MQ=255)

       tAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAAc                                                                                                                                     <  2:178141/141‑1 (MQ=255)

        aaaCTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTAC‑TTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACaa                                                                                                                                   <  1:302069/141‑1 (MQ=255)

         aaCTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACaa                                                                                                                                   >  1:683936/1‑141 (MQ=255)

         aaCTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACaa                                                                                                                                   <  1:683385/141‑1 (MQ=255)

           ctgcggtACGTTGCTCACCAAACCTCTTTTGTTTAATAAAAGATGGAGAAGTCAGAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACTAACAAga                                                                                                                                 <  2:842074/136‑1 (MQ=255)

            tGGTGTACGTTGCTTACCAAACCACTTTTGTTTAATAAAGGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTTCATAACGCTCAATAAGTAATAATAAAAAGACGAACAAgat                                                                                                                                <  2:612778/141‑1 (MQ=255)

              gtgtACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAgatga                                                                                                                              >  1:301634/1‑141 (MQ=255)

              gtgtACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTATGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAgatga                                                                                                                              >  1:515309/1‑141 (MQ=255)

               ggtACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAgatgat                                                                                                                             <  1:4485/140‑1 (MQ=255)

                 tACGTTGCTCACCAAACCACTTTTGTTTGAAAACAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTGTGACTTTCGTATTACATCACGCACAATAAGTAATAATAAACAGACGAACAAaatgacga                                                                                                                           >  2:792559/1‑141 (MQ=255)

                 tACGTTGCTCACCAAACCACTTTTGCTTAAAAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAgatgatga                                                                                                                           <  1:984912/141‑1 (MQ=255)

                     ttGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGt                                                                                                                       >  2:996799/1‑141 (MQ=255)

                     ttGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGt                                                                                                                       >  2:975887/1‑141 (MQ=255)

                       gCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGtat                                                                                                                     <  2:415142/141‑1 (MQ=255)

                       gCTCACCAAACCACTTTTGTTTAATAAAAGATGCTGATGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAAATAACAGAAGAGCAAGATGATAAttaaat                                                                                                                     >  2:788059/1‑137 (MQ=255)

                        cTCACCCAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACGACTTTTTTTGGTTTAAACTTGTGGATTAAATATCGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGtata                                                                                                                    <  2:782110/141‑1 (MQ=255)

                             cAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCCCAATCAGTACTAATAAAAAAACGAACAAGATGATGATTGTATACttat                                                                                                               >  1:513007/1‑141 (MQ=255)

                             cAAACAACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGGAATAATAACAAGACGAACAAGATGATGATGGTATACttat                                                                                                               >  1:514572/1‑141 (MQ=255)

                               aaCCGCTTTTGTTTAATAAAAGATGGTCAAGTCATAACCTCATCCCTCGCAACTACTTGTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACttatta                                                                                                             <  1:589981/141‑1 (MQ=255)

                                 ccACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGGACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGa                                                                                                           <  1:1134821/141‑1 (MQ=255)

                                    cTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACaa                                                                                                        >  2:619996/1‑141 (MQ=255)

                                     ttttGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACaat                                                                                                       <  1:744328/141‑1 (MQ=255)

                                     gtttGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACaat                                                                                                       <  2:585596/140‑1 (MQ=255)

                                            taataaAAGATGGTGAAGTCATAACCTCATCCTTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGa                                                                                                >  1:1187134/1‑141 (MQ=255)

                                            taataaAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACGGACGAAGAAGATGATGATTGTATCCTTATTAGACAATAATGTGa                                                                                                >  1:195472/1‑141 (MQ=255)

                                            taataaAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTAATAGACAATAATGTGa                                                                                                >  2:1000738/1‑141 (MQ=255)

                                             aataaAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGAACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGaa                                                                                               <  2:201005/141‑1 (MQ=255)

                                              ataaAAGATGGTGAAGTCATCAGACGATCCGTCGCAACTACTTTTTTTGGTATAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAAt                                                                                              >  2:849108/1‑141 (MQ=255)

                                              ataaAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAAt                                                                                              >  1:1104423/1‑141 (MQ=255)

                                              ataaAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGAGGATTGTATACTTATTAGACAATAATGTGAAt                                                                                              >  1:88331/1‑141 (MQ=255)

                                                aaaaGATGGTGAAGTCATAACATCATCCGTCGCAACTAC‑TTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCa                                                                                           >  2:547570/1‑141 (MQ=255)

                                                    gATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAAtt                                                                                        <  2:831117/141‑1 (MQ=255)

                                                     aTGGTGAATTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTa                                                                                       <  2:815930/141‑1 (MQ=255)

                                                      tGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTGGTGTGTTAAATAATGCACAATAAGTAATAATAAAAAGGCGAACGAGATGATGATAATATACTTATTAGCCGAAAGTGTGAATGAAATTaa                                                                                      >  1:1146049/1‑141 (MQ=255)

                                                       ggTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGCCAATAATGTGAATGCAATTAAt                                                                                     <  2:875719/141‑1 (MQ=255)

                                                       ggTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAAt                                                                                     <  1:855461/141‑1 (MQ=255)

                                                       ggTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAAt                                                                                     >  1:1125954/1‑141 (MQ=255)

                                                        gTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATa                                                                                    >  1:1031456/1‑141 (MQ=255)

                                                        gTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACACACAATAAGTAATAATAACAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATa                                                                                    >  2:770840/1‑141 (MQ=255)

                                                         tGAAGTCATAACCTCATCCGTCGCAACTAATTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAGTGTGAAGGCAATTAATAg                                                                                   >  1:342340/1‑141 (MQ=255)

                                                         ggacttCATAACCTTATCCGTCGAAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAg                                                                                   <  2:275211/136‑1 (MQ=255)

                                                          gAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAgc                                                                                  >  1:941571/1‑141 (MQ=255)

                                                            aGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAgcgc                                                                                <  1:299136/141‑1 (MQ=255)

                                                               taTAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGcc                                                                             <  2:739249/140‑1 (MQ=255)

                                                               cATACCCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTCAGTAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGcc                                                                             <  1:33408/141‑1 (MQ=255)

                                                                aTAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGCTTGTCAACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCt                                                                            <  2:595462/141‑1 (MQ=255)

                                                                 tAACCTCATCCGTCTCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTa                                                                           <  2:1119635/141‑1 (MQ=255)

                                                                 tAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTa                                                                           <  1:990318/141‑1 (MQ=255)

                                                                 tAACCTCAGCCGTCGCAACTACTGTTTTTGGGTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTa                                                                           <  2:187900/141‑1 (MQ=255)

                                                                          ccGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACg                                                                  >  1:597100/1‑141 (MQ=255)

                                                                          ccGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACg                                                                  >  1:131514/1‑141 (MQ=255)

                                                                            gTCGCAACGACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGcc                                                                <  1:1133618/141‑1 (MQ=255)

                                                                            ctCGCAACTACTTTTTTTGGGTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGcc                                                                <  1:1133590/140‑1 (MQ=255)

                                                                             tCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCa                                                               <  1:989389/141‑1 (MQ=255)

                                                                                cAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATCTTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCaaaa                                                            <  1:996799/141‑1 (MQ=255)

                                                                                      ctTTTTTTGATTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCAc                                                      <  2:805504/141‑1 (MQ=255)

                                                                                       tttttttGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAATAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACg                                                     <  1:617332/141‑1 (MQ=255)

                                                                                        ttttttGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGt                                                    <  1:24641/141‑1 (MQ=255)

                                                                                         tttttGTGTTAAACCTGTGTATTAAATAACGCACAAGAAGTAAAAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAAGAATCTGAAGGCAATTAATAGCGCGCCTCTCCAAAACGCCAAAAGGCCACGTa                                                   <  2:560717/141‑1 (MQ=255)

                                                                                          ttttGGTTTAAACTTGTGTATACAGAAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCACAACGCCAAAAGGCAACGTAt                                                  >  2:1157123/1‑141 (MQ=255)

                                                                                             tGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGACATAACAACCGCGCCTGTGCAAAACGGCAAAAAGCGACAGAtttt                                               >  2:630797/1‑141 (MQ=255)

                                                                                               gTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAAACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGACTGCACGTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTAt                                             <  2:785865/141‑1 (MQ=255)

                                                                                               gTTTAAACTTCTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTAt                                             <  2:798988/141‑1 (MQ=255)

                                                                                                          tgtATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGa                                  >  2:1084759/1‑141 (MQ=255)

                                                                                                          agtATAAAAAAACAGACACGAAGTAATAAGAAAGAGAGAAACAAGATGATGACTGTATGCTTATTAGACAGTAATGTGAATGCAATTAATTGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGa                                  <  2:224995/140‑1 (MQ=255)

                                                                                                           gtATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGat                                 <  2:1034565/141‑1 (MQ=255)

                                                                                                            tATTAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGata                                <  2:1187134/141‑1 (MQ=255)

                                                                                                            gaTTAAAGAACGCACAATAAGTAATAATAAACAGACGAACAGGATGACGATTTTATACCTATTAGACAATAATGTGAATGCAATTAATAGGGCGCCGACTCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGata                                <  2:195472/140‑1 (MQ=255)

                                                                                                              ttAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATc                              <  1:433124/141‑1 (MQ=255)

                                                                                                              ttAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATc                              <  1:22531/141‑1 (MQ=255)

                                                                                                               tAAATAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATGGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTAATTTATGAACATAAAGATATcc                             <  1:619996/141‑1 (MQ=255)

                                                                                                                aaaTAACGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCt                            >  1:709382/1‑141 (MQ=255)

                                                                                                                aaaTAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAAT‑ATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAAATATCCTa                           >  2:766694/1‑141 (MQ=255)

                                                                                                                  aTAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTAc                          <  2:796102/141‑1 (MQ=255)

                                                                                                                      cGCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATaa                      >  2:1033568/1‑141 (MQ=255)

                                                                                                                       gCACAATAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATaaa                     >  2:593503/1‑141 (MQ=255)

                                                                                                                         acaATAAGTAAGTAGAAAGAGACGAATAAGCTGCTGATTGTATACCTATCAGAAAATAATGTGAATGCAATTAATAGCGCGCCTACGCAAAGCCCCAAAAGGCCACGTAGTTTATTAAAATAAAGATATCCTACATAAACt                   <  2:901640/141‑1 (MQ=255)

                                                                                                                           aaTAAGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTGCATAAACTaa                 <  2:812271/141‑1 (MQ=255)

                                                                                                                             tAAGTAATAATAAACTGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATa               >  1:600914/1‑141 (MQ=255)

                                                                                                                             tAAGTAATAACAAACAGACCAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATa               <  2:278506/141‑1 (MQ=255)

                                                                                                                              aaGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATac              >  2:537991/1‑141 (MQ=255)

                                                                                                                              aaGTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATac              >  1:1070496/1‑141 (MQ=255)

                                                                                                                                gTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATacac            >  1:1131439/1‑141 (MQ=255)

                                                                                                                                gTAATAATAAACAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATacaa            >  1:1145944/1‑140 (MQ=255)

                                                                                                                                gTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATacac            <  1:478033/141‑1 (MQ=255)

                                                                                                                                ctaataataaACAGACGAACAAGAGGATGATTGTATACCTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTACCCAAAACTCCAAAAGGCCACGTATTTTATGAAAACAAAGATATCCTACATAAACTAATacac            <  2:1085196/140‑1 (MQ=255)

                                                                                                                                ataataataaACAGACGAACAAGATGATGATCGTATACATATTAAACAATAATGTGAATGCAAATAATAGCGCCCCTATCCAAAACGCCAAAAGGCCACGTATTTTACGAAAATAAAGATATCCTACATAAACTAATacac            <  2:833775/140‑1 (MQ=255)

                                                                                                                                          aaaGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCaataa  >  1:30463/1‑141 (MQ=255)

ATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAACAAGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAA  >  USA300TCH1516_ALE/2028255‑2028533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`