Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,028,583 G→T 25.2% A21D (GCC→GAC)  USA300HOU_RS10230 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,028,5830GT25.2% 130.2 / 34.1 72A21D (GCC→GAC) USA300HOU_RS10230hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/26);  new base T (7/11);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

AATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTG  >  USA300TCH1516_ALE/2028444‑2028723
                                                                                                                                           |                                                                                                                                            
aaTTAATATCGCGCCCATCCAAAACGCCAAAAGGCCACGTATGTCATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGc                                                                                                                                             <  1:618521/141‑1 (MQ=255)
aaTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGc                                                                                                                                             <  1:791307/141‑1 (MQ=255)
 aTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCa                                                                                                                                            <  2:36602/141‑1 (MQ=255)
  ttAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaa                                                                                                                                           <  1:1000738/141‑1 (MQ=255)
    aaTCGCGCGCCTATCAAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGCGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaata                                                                                                                                         <  1:500496/141‑1 (MQ=255)
    aaTAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaata                                                                                                                                         >  1:781495/1‑141 (MQ=255)
     aTAGCGCGCCATTCCAAAACGCCAAAAGGCCACGCATTTCATGAAAATAAAGATAACCTACATAAACTAGTACACTTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaataa                                                                                                                                        <  2:342340/141‑1 (MQ=255)
      tAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaataat                                                                                                                                       >  2:498686/1‑141 (MQ=255)
      tAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAAAATGTAGTAAAAATTGGCaataaa                                                                                                                                       >  2:703799/1‑140 (MQ=255)
       aGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCaataata                                                                                                                                      >  1:106861/1‑140 (MQ=255)
       aGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATc                                                                                                                                      <  1:434513/141‑1 (MQ=255)
            gcCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTaa                                                                                                                                 <  2:611203/141‑1 (MQ=255)
                aTCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATCGAACGAGTAAAAAGACAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCa                                                                                                                             <  1:792559/141‑1 (MQ=255)
                  ccAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGt                                                                                                                           <  1:1157123/141‑1 (MQ=255)
                     aaaCGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAACATATGTAGTAAAAATGGTCAATAATCATTAGTGCAGTaaa                                                                                                                        >  2:1155974/1‑141 (MQ=255)
                     aaaCGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTaaa                                                                                                                        >  1:18688/1‑141 (MQ=255)
                          ccAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGa                                                                                                                   >  2:830321/1‑141 (MQ=255)
                             aaaGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCa                                                                                                                >  1:500553/1‑141 (MQ=255)
                               aGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAAc                                                                                                              <  2:1070496/141‑1 (MQ=255)
                                ggCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGCGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             >  2:15078/1‑141 (MQ=255)
                                ggCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             <  2:941571/141‑1 (MQ=255)
                                ggCCAACTATTTTAAGAAAATAAAGATATCCTACATAAACTAATACACATCCAAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAACATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             <  2:301634/141‑1 (MQ=255)
                                 ggcACGTAATTAATAAAACTAAATATATCCTACAATAACTCATACACTGTCCAATAATAGACCGAGGAAAAAGCAAAATTTTTCAAAAAATATGGAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAActt                                                                                                            <  2:514572/139‑3 (MQ=255)
                                    aCGTATTTTATTAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTg                                                                                                         <  2:1163018/141‑1 (MQ=255)
                                    aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTg                                                                                                         >  2:687575/1‑141 (MQ=255)
                                    aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACAtt                                                                                                           <  1:765765/139‑1 (MQ=255)
                                    aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACAtt                                                                                                           >  2:765765/1‑139 (MQ=255)
                                       tATTTTATGAAAATAAAGATATCCTACATAAACTAATACAAATCCCAATAATAGAACGAGTAAAAAGCAAATTTTTTCAAAAAATATGTAGTAAAAATAAGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGt                                                                                                      >  2:563022/1‑141 (MQ=255)
                                        aTTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGtt                                                                                                     >  2:459082/1‑141 (MQ=255)
                                                aaaaTAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGatatata                                                                                             >  1:563975/1‑141 (MQ=255)
                                                        gATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTtgtgtg                                                                                     <  1:834729/141‑1 (MQ=255)
                                                        gATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTtgtgtg                                                                                     <  2:172476/141‑1 (MQ=255)
                                                          tatCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTc                                                                                   >  1:396381/1‑141 (MQ=255)
                                                          tatCCTACAGAGACTAAGACACATCCCAATGATTGGACGAGTAAAAAGGCAATTTTGTCAAAAAATATGTAGTAAAAATTGGCAATGGGCATTAATGCAGTAAAAATGATCGACAGGTGCGTTGATATATATTTGTGTGTc                                                                                   <  2:157/141‑1 (MQ=255)
                                                             ccTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCCAAAAATATGTAGTAAAAATCGCCAATAATCATTAATGCAGTAAAAATGATCAACATTGGCGTTGATATATCTTTTTATGTCATg                                                                                >  2:740698/1‑141 (MQ=255)
                                                                aCATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGttt                                                                             >  1:198434/1‑141 (MQ=255)
                                                                 cATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  1:849108/141‑1 (MQ=255)
                                                                 cATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  2:841132/141‑1 (MQ=255)
                                                                 cATAAACTAATACACATCACAATAATAGAACGAGTAAAAAGCCAATATAATAAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  2:640480/141‑1 (MQ=255)
                                                                     aaCTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTcgccg                                                                        >  1:630049/1‑141 (MQ=255)
                                                                          aTACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCttt                                                                   >  1:303904/1‑141 (MQ=255)
                                                                              acaTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTAt                                                               >  1:1038711/1‑141 (MQ=255)
                                                                              acaTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTAt                                                               >  1:988558/1‑141 (MQ=255)
                                                                               caTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTAGCCGCCTTTCTATg                                                              >  1:278584/1‑141 (MQ=255)
                                                                                aTCCCAATAATAGAACGAGTAAAAAGCAAAAATATTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGc                                                             <  2:247913/141‑1 (MQ=255)
                                                                                 tCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGGGTCATGTTTGGCCGCCTTTCTTTGcc                                                            >  2:983717/1‑141 (MQ=255)
                                                                                 cccaaaaaataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTTGCCGCCTTTCTATGcc                                                            <  1:593503/134‑1 (MQ=255)
                                                                                   ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac                                                          <  1:215268/141‑1 (MQ=255)
                                                                                    cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAACTATGTCGTTTTAATTTGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca                                                         >  1:934514/1‑141 (MQ=255)
                                                                                     aataatAGACCGATGAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:88331/141‑1 (MQ=255)
                                                                                     aataatAGAAGGAGTAAAAAGCAAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:966570/141‑1 (MQ=255)
                                                                                     aataatAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATACGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:598600/141‑1 (MQ=255)
                                                                                      ataataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCTCCTTTCTATGCCACAGc                                                       >  2:268063/1‑141 (MQ=255)
                                                                                         ataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGtt                                                    >  2:276414/1‑141 (MQ=255)
                                                                                           aGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGAAAAAATGATCAACATTTTCTTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCa                                                  <  2:513007/141‑1 (MQ=255)
                                                                                            gAACGAGAAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCat                                                 <  1:678099/141‑1 (MQ=255)
                                                                                             aaCGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCata                                                <  2:1125954/141‑1 (MQ=255)
                                                                                              aCGAGTAAAAAGCCAATTTTTTCAAAAAAGATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCatat                                               <  2:30513/141‑1 (MQ=255)
                                                                                                  gTAAAAAGCCAATTGTTGCAAAAAATGTGGAGGGGAAAGTGTCAATAATCAGTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAtt                                           >  2:1187628/1‑141 (MQ=255)
                                                                                                   tAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAttt                                          <  1:1136909/141‑1 (MQ=255)
                                                                                                   tAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAttt                                          <  1:687575/141‑1 (MQ=255)
                                                                                                    aaaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTACTGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATtta                                         >  1:692953/1‑141 (MQ=255)
                                                                                                    aaaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTGAGGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATtta                                         >  1:692970/1‑141 (MQ=255)
                                                                                                         gCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGCGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGa                                    >  2:818356/1‑141 (MQ=255)
                                                                                                          ccAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGAt                                   >  1:467114/1‑141 (MQ=255)
                                                                                                               ttttgCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGAt                              <  2:242495/141‑1 (MQ=255)
                                                                                                                ttttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATg                             >  2:1011528/1‑141 (MQ=255)
                                                                                                                    cAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGtttt                         <  1:537991/141‑1 (MQ=255)
                                                                                                                     aaaaaaTATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGttttt                        <  2:218368/141‑1 (MQ=255)
                                                                                                                             tGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGc                >  1:851358/1‑141 (MQ=255)
                                                                                                                              gtagtaAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCGCAGCGTTCATATAATTTCTTAGATACGATGTTTTTTCTGTTGCt               >  1:563556/1‑141 (MQ=255)
                                                                                                                                      aaTTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTc       >  1:659700/1‑141 (MQ=255)
                                                                                                                                          ggCAATAATAATTAATGCAGTAAAAATGATCAACATATGCGTTGATAAATATTTGTGTGTCATGTTTAGCCGCCTTTCTATGCCACAGCGTTAATATACTTTATTAGATACGATGTTTTATCTGTTGCTATTTTTTCAGtt   >  2:222718/1‑141 (MQ=255)
                                                                                                                                           gCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTg  <  2:753196/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTG  >  USA300TCH1516_ALE/2028444‑2028723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑