BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,352,815	G→A	27.9%	A92V (GCA→GTA)	rpsI ←	30S ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,352,815	0	G	A	27.9%	191.8 / 81.7	124	A92V (GCA→GTA)	rpsI	30S ribosomal protein S9
Reads supporting (aligned to +/- strand): ref base G (44/45); new base A (18/17); total (62/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 125 total aligned reads displayed.

ATATATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAATTAA  >  USA300TCH1516_ALE/2352675‑2352954

atatatCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGAGCGTTTTAAAGAACCTCTGTCTTCAGGATCTg                                                                                                                                             >  2:80627/1‑141 (MQ=255)

   tatCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCtt                                                                                                                                          >  1:522056/1‑141 (MQ=255)

   tatCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATACAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCtt                                                                                                                                          >  1:111456/1‑141 (MQ=255)

          cGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCGTCtaataa                                                                                                                                   >  2:1049568/1‑141 (MQ=255)

          cGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGGAGGTAATCCAGCGCGTTTTAAAGAACCTCTGTATGCAGGGTCTGCTTCtagtaa                                                                                                                                   >  2:1154252/1‑141 (MQ=38)

           gACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGGTTTAAGGCCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTGAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCGGGATCTACTTCtaataca                                                                                                                                  >  2:1094023/1‑139 (MQ=38)

             cAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTGTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGc                                                                                                                                >  2:902211/1‑141 (MQ=255)

              aaTTAACGTTTTGAGCATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCa                                                                                                                               >  1:884931/1‑141 (MQ=255)

              aaTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCa                                                                                                                               >  2:649955/1‑141 (MQ=255)

               aTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCAc                                                                                                                              >  2:873071/1‑141 (MQ=255)

                ttAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACg                                                                                                                             >  1:715488/1‑141 (MQ=255)

                 tAACGTTTTGAGAATTGAGGTGAACAGAAATCGGCATTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGCTCTACTTCTAATAATGCACgg                                                                                                                            >  2:1099669/1‑140 (MQ=255)

                  aaCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCTGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAg                                                                                                                           <  1:366144/141‑1 (MQ=255)

                  aaCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAg                                                                                                                           >  2:925544/1‑141 (MQ=255)

                  aaCGTTGTGAGAATTGAGGTGAACAACGCGCTGCCTTAAGACCCGCTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAg                                                                                                                           <  1:158042/141‑1 (MQ=255)

                   aCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGc                                                                                                                          >  1:115935/1‑141 (MQ=255)

                     gTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGa                                                                                                                        >  2:160753/1‑141 (MQ=255)

                       tttGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGAtt                                                                                                                      >  2:840543/1‑141 (MQ=255)

                        ttGAGAATGGAGGCGAACGACGAGCAGCTTTAAGACCTGGTTTTTCATGTTCATTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTc                                                                                                                     <  1:291364/141‑1 (MQ=255)

                         ttagaATTGAGGTGAACGACGAACTCGATGAAAACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTcc                                                                                                                    >  1:880069/3‑141 (MQ=255)

                         tGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATGCAGGATCTGCTTCTAATAATGCACGAGCGATTcc                                                                                                                    <  2:153763/141‑1 (MQ=255)

                         tGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTAGTTTTCTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAATAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGGTTcc                                                                                                                    <  1:843380/141‑1 (MQ=39)

                             aaTTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAAGAATGCACGAGCGATTCCGTGa                                                                                                                <  1:615225/141‑1 (MQ=255)

                              aTTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATCATGCACAAGCGATTCCGTGAc                                                                                                               <  2:820416/141‑1 (MQ=255)

                                tGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTACGTGACgg                                                                                                             >  2:487708/1‑141 (MQ=255)

                                 gAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGCGTAAGTAATCCAGCGCGTTTTAAATAACCTCTGTATTCAGGATCTACTTCGAATAATGCACGAGCGATTCCGTGACGGa                                                                                                            <  1:775350/141‑1 (MQ=255)

                                   ggTGAACGACGAGCTGCTTGAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATa                                                                                                          >  1:657548/1‑141 (MQ=255)

                                    gTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAACTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATag                                                                                                         <  1:805765/141‑1 (MQ=255)

                                         cgacgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCGGATAAGGCACGAGCGATTCCGTGACGGATagcttga                                                                                                    <  2:235877/141‑1 (MQ=255)

                                           acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagc                                                                                                  <  1:347862/141‑1 (MQ=255)

                                             gaGCTGCTTTAAGACCTGGTTGTTTACTTTCTTTCATACGTGCGTCACGACTAAGTAAGCGCGAGAGTTTTAACGAACTTCTGTATTCATGATCTGCTTTGAATAAGGCACTGCCGATTCCGTGACGGATagcttgggttt                                                                                                <  2:359438/141‑6 (MQ=255)

                                               gctgatTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGt                                                                                              <  1:109223/141‑1 (MQ=255)

                                                ctgctTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTc                                                                                             <  1:461486/141‑1 (MQ=255)

                                                      tAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGa                                                                                       >  2:77057/1‑141 (MQ=255)

                                                       aaGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGGGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaa                                                                                      <  2:73694/141‑1 (MQ=255)

                                                       aaGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaa                                                                                      <  1:443505/141‑1 (MQ=255)

                                                       aaGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaa                                                                                      <  1:533841/141‑1 (MQ=255)

                                                         gACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGCAac                                                                                    >  1:294937/1‑141 (MQ=255)

                                                            cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccac                                                                                 >  1:154727/1‑141 (MQ=255)

                                                            cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccac                                                                                 >  1:969125/1‑141 (MQ=255)

                                                            cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGGGAAaccac                                                                                 >  1:1082609/1‑141 (MQ=255)

                                                            cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccac                                                                                 >  1:841504/1‑141 (MQ=255)

                                                            cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccac                                                                                 >  2:373157/1‑141 (MQ=255)

                                                             tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                                <  2:52634/141‑1 (MQ=255)

                                                              ggTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacca                                                                               <  1:373157/141‑1 (MQ=255)

                                                              ggTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGAGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATGCCGTGACGGGTAGCTTGAGGTTGTCCAGTGAAagcacca                                                                               >  1:102751/1‑141 (MQ=255)

                                                              ggTTTTTTACGTTCCTTCAGACGTGGGTCATGAGCATTTAATCCATCCCGTCTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGGGCGATTCCGCTACTGATAGCTTGACCTTGTCCAGTGAAaccaccc                                                                               <  2:218584/141‑2 (MQ=255)

                                                                ttttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCGGTGACGGATAGCTTGAGCTGGTCCAGTGGAaccaccgcc                                                                             >  2:330087/1‑141 (MQ=255)

                                                                ttttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccacc                                                                             >  2:293213/1‑141 (MQ=255)

                                                                ttttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccacc                                                                             <  1:849236/141‑1 (MQ=255)

                                                                  ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCAt                                                                           <  1:840543/141‑1 (MQ=255)

                                                                   tttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATg                                                                          <  2:393855/141‑1 (MQ=255)

                                                                      aCGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGCCGGATAGCTTGGGCTTGTCCAGTGCAACCACCACCATGAAc                                                                       >  2:356902/1‑141 (MQ=255)

                                                                             ttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                                >  2:475919/1‑141 (MQ=255)

                                                                               cATACGTTGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACTTCTGTATTCAGTATCTGCTTCTAATAATGCACGAGCGATTCCGTCACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                              <  2:264901/141‑1 (MQ=255)

                                                                               cATACGTTGCGCACGAGTAAGTAATCCTGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                              <  1:740274/141‑1 (MQ=255)

                                                                                 tACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaaaa                                                            >  1:948427/1‑141 (MQ=255)

                                                                                   cttGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAca                                                          <  2:692502/139‑1 (MQ=255)

                                                                                     taggacACGAGTAAGTAATCCAGCGCGTTTTAAAGGAGCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGCGCTTGTCCAGTAATACCACCTCCATCAAGGTTAACTAAACcatc                                                        >  2:539221/6‑141 (MQ=255)

                                                                                     tGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatc                                                        >  2:428697/1‑141 (MQ=255)

                                                                                     ggggTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatc                                                        <  1:116763/140‑1 (MQ=255)

                                                                                      gggTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATACCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatca                                                       <  2:881865/141‑1 (MQ=255)

                                                                                         tCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAg                                                    >  2:184613/1‑141 (MQ=255)

                                                                                           aCGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCCACTTCTAATAATGCACGCGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGtt                                                  >  1:310004/1‑141 (MQ=255)

                                                                                           aCGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAAGATCTACTTCTAGTAATGCACGAGCGATTCCGTGACGGATCGCTTGGGCTTGTCCAGGGAAACCACCACCATGAACGTTAACTAAAACATCATAGtt                                                  >  1:408960/1‑141 (MQ=255)

                                                                                                taagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCGCGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCGGTGAAACCACCACCATGGACGTTAACTAAAACATCAAAGTTAccgt                                             >  1:539561/1‑139 (MQ=255)

                                                                                                taagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCCCCACCATGAACGTTAACTAAAACATCATAGTTACCtt                                             >  2:740576/1‑141 (MQ=255)

                                                                                                 aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCttt                                            >  1:1057699/1‑141 (MQ=255)

                                                                                                 aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCATATAATAATGCACGAGCCATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACACAAACAGCATAGTGACGttt                                            >  2:299599/1‑141 (MQ=255)

                                                                                                 aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAAGGACGACCAAAAGATCATATTTACCttt                                            >  2:297791/1‑141 (MQ=255)

                                                                                                  agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGCTAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACAACCTAGTTACCATTa                                           >  1:570292/1‑141 (MQ=255)

                                                                                                     aaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTTTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGtt                                        <  2:1028503/141‑1 (MQ=255)

                                                                                                       tCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTc                                      >  1:547432/1‑141 (MQ=255)

                                                                                                        ccAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCa                                     >  1:292721/1‑141 (MQ=255)

                                                                                                          aGCGCGTTTTACAGCACCCCTGTAGTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTCAAACATCATAGTTACCATTAGTTTCAGt                                   >  2:1031286/1‑141 (MQ=255)

                                                                                                           gcgcGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACAACATAGTTACCTTCAGGTTCAGtt                                  >  2:207773/1‑141 (MQ=255)

                                                                                                             gcgTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTAc                                <  1:202687/141‑1 (MQ=255)

                                                                                                             gcgTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTAc                                <  1:407973/141‑1 (MQ=255)

                                                                                                             gcgTTTTAAAGAACCTCTGTATTCAGGAGCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTAc                                <  1:831572/141‑1 (MQ=255)

                                                                                                               gTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACAt                              <  2:1044812/141‑1 (MQ=255)

                                                                                                                  ttAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaa                           >  1:1067344/1‑141 (MQ=255)

                                                                                                                    aaaGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTGACTAAAACATCATAGTTACCTTTAGTTTGAGTTACATCAAAt                         >  2:320371/1‑141 (MQ=255)

                                                                                                                      atcaCCGCTGTATTTGGGATCGGCTTCTAAGCATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtgg                       <  2:773867/138‑1 (MQ=255)

                                                                                                                      aGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtgg                       <  1:584054/141‑1 (MQ=255)

                                                                                                                       gAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggt                      <  2:154727/141‑1 (MQ=255)

                                                                                                                       gAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggt                      <  1:77057/141‑1 (MQ=255)

                                                                                                                         aactctGTATTCAGGATCTACTTCTAATAAGGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg                    <  1:1094023/139‑1 (MQ=255)

                                                                                                                          ccTCTGTCTTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg                   <  1:649955/141‑1 (MQ=255)

                                                                                                                           ctctGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGCTTCAGTTACATCAAAtggttggt                  >  2:690014/1‑141 (MQ=255)

                                                                                                                              tGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTGGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAATTTATTCTTCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTa               <  2:657548/141‑1 (MQ=255)

                                                                                                                                tATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAg             <  1:160753/141‑1 (MQ=255)

                                                                                                                                  ttCAGGATGTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTc           <  2:815670/141‑1 (MQ=255)

                                                                                                                                    cAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTa         >  1:271406/1‑141 (MQ=255)

                                                                                                                                    cAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTa         >  1:1087002/1‑141 (MQ=255)

                                                                                                                                     aGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGCTTCAGTTACATCAAATGGGTGGTTTAAGTCTaa        >  1:103532/1‑141 (MQ=255)

                                                                                                                                     aGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTaa        <  1:873449/141‑1 (MQ=255)

                                                                                                                                      ggATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTaaa       >  1:57311/1‑141 (MQ=255)

                                                                                                                                         tCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAAtt    >  1:1174874/1‑141 (MQ=255)

                                                                                                                                           tGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTCCATCAAATGGTTGGTTTAAGTCTAAAATTaa  >  2:424045/1‑141 (MQ=255)

                                                                                                                                           tGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAATTaa  <  1:873071/141‑1 (MQ=255)

ATATATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAATTAA  >  USA300TCH1516_ALE/2352675‑2352954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`