| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,455,692 | Δ1 bp | coding (1210/1260 nt) | lyrA → | Lysostaphin resistance protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,455,692 | 0 | G | . | 88.2% | 398.1 / 51.3 | 110 | coding (1210/1260 nt) | lyrA | Lysostaphin resistance protein A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (47/50); minor base A (10/3); total (57/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
AGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTA > USA300TCH1516_ALE/2455559‑2455826 | agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatt > 2:614593/1‑139 (MQ=255) cagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattat < 2:338631/141‑5 (MQ=255) gAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcg < 2:391235/141‑7 (MQ=255) aagattgaaaCAAGCATCAACAAGGCACGATTTAACAAACGATTCAAATGAAGATGAAATAGATCAGTCATTAAAAGAACCTGCCA‑CTTATAAAGAAGACCGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGaa < 2:60996/134‑1 (MQ=255) tCAATTAAACAAGCATCACAAAGTGACGACTTAACAAACGATTCAAATGAAAATGGAACAGAGCAATCATTAAAAGAACCTGCCATGTTAT‑TAGATGACAGATTTTCATTAGTTGTAATTGATGCA‑AAAAAATTATCGaaa < 2:162505/141‑1 (MQ=255) aGTTGAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGaaaaa > 2:685656/1‑141 (MQ=255) aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaa < 1:890914/141‑1 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGaaaaaa > 1:989154/1‑141 (MQ=255) gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGCTGTAATTGAGACA‑AAAAAATTATCGaaaaaa > 2:245225/1‑141 (MQ=255) aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTg < 1:57295/141‑1 (MQ=255) aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAAGAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTCGTAATTGATGCA‑AAAAAATTATCGAAAAACCTg < 2:365554/141‑1 (MQ=255) aaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTga > 1:801376/1‑141 (MQ=255) aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaaga < 2:404982/141‑1 (MQ=255) aaGCACCACAAAGTGACGATTTAACAAACGAATCAAATGCAGTTGAAATAGAACAATCATTAAAATAACCTCCGG‑CTTATAAGGAAGGCAGACGGTCAACAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaaga < 2:977482/141‑1 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAGAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAAc > 1:429628/1‑141 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTgaagaaa > 1:958761/1‑140 (MQ=255) gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAc > 1:896097/1‑141 (MQ=255) aaccacAAAGTTACGAATTAACAAACGACCAAAACGAAGATCGAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTACAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACa < 1:512088/140‑1 (MQ=255) cccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACaa < 1:314202/140‑1 (MQ=255) accacAAAGTAACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACaa < 1:88133/141‑1 (MQ=255) ccacAAAGTGACGATTTAACAAACAAATCAAATGAAGATGAAATAGAGGAATAATTAAACGAACCTGCGT‑CTTATAAAGAGGACAGACGTTCATCAGTAGTAATAGATGCA‑AAACAAAAATCGAAAAAACTGAAGAATaaa > 2:661700/1‑140 (MQ=255) cacaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATc > 2:777413/1‑141 (MQ=255) cacaAAGTGACGAGTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATc > 2:513165/1‑141 (MQ=255) acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAGTAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCt > 2:903368/1‑141 (MQ=255) acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCt > 2:987170/1‑141 (MQ=255) aaaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTc > 1:440877/1‑141 (MQ=255) aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAg > 1:367635/1‑141 (MQ=255) gTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTGTCGAAAAAACTGAAGAACAATCTTCAGa > 2:412123/1‑141 (MQ=255) aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaa > 1:782854/1‑141 (MQ=255) gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa < 1:11581/141‑1 (MQ=255) aaaCAAACGATGCAAATGAAGATGAAATAGTGCAATCATGAAAAGAACCCGCGA‑CTTATAAAGAAGACAGACGTTCAACAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACaa < 2:290145/140‑1 (MQ=255) aaCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAAAAATCTTCAGATaaaaaacaa > 2:881796/1‑137 (MQ=255) aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat < 1:142799/141‑1 (MQ=255) tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTACTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaa > 1:663633/1‑141 (MQ=255) tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATA‑ATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaa > 2:499982/1‑141 (MQ=255) tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATACTAACaaa > 1:663821/1‑141 (MQ=255) tGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCt < 1:367344/141‑1 (MQ=255) gATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt < 1:741046/141‑1 (MQ=255) gATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt < 1:915490/141‑1 (MQ=255) tAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAATGCGCTTGCTAGTACct < 2:387351/141‑1 (MQ=255) tAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 1:214358/141‑1 (MQ=255) tAGAGCAATCAGTAAAAGAACCCGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 2:105190/141‑1 (MQ=255) aagagCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct < 1:262224/140‑1 (MQ=255) agCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt > 1:576323/1‑141 (MQ=255) agCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt > 2:10389/1‑141 (MQ=255) cAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTACTTGAAGCA‑AAAAAATTATCGAAAAAACTGAAGAACACTCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa > 1:276768/1‑141 (MQ=255) cAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa > 2:595765/1‑141 (MQ=255) aactaatAAAACAACCTTCTA‑CTGAGAAACAAGACAGACGTTCATCAGTTGGAATTGATGCA‑AAAAAAAAAACAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa < 2:443134/135‑1 (MQ=255) aTCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa < 1:305091/141‑1 (MQ=255) ttAAAAGCACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat < 1:158367/141‑1 (MQ=255) ttAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat < 1:96301/141‑1 (MQ=255) ttAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat < 1:987170/141‑1 (MQ=255) tAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatg < 1:317032/141‑1 (MQ=255) aaaGGACCTGCGA‑CGTCAAAAGACGACAGACTTTCACCAGTTGTAATTGATGCA‑AAGCAATTACCAAAAACCCTGAAGAACAATCTTCATATAAAAACCAATAATAACAAAGCGCTTGCTAGTACCACTTAAAAAgatcat > 2:322428/1‑141 (MQ=255) aaaGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatgat < 2:312485/141‑1 (MQ=255) aaGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg > 1:854226/1‑141 (MQ=255) ccTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc > 1:18589/1‑141 (MQ=255) ccTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc > 1:18508/1‑141 (MQ=255) ccTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc > 1:701874/1‑141 (MQ=255) cTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa < 1:114791/141‑1 (MQ=255) gCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAACTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGCTGCTAGCaaa > 2:673765/1‑140 (MQ=255) a‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc < 1:614593/141‑1 (MQ=255) a‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCACTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc < 2:383190/141‑1 (MQ=255) cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt < 1:307182/141‑1 (MQ=255) tataACGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCttt < 2:445082/141‑1 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAACAGATGATGCTAGCAAGCGCtttt > 1:610639/1‑141 (MQ=255) ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt > 1:828945/1‑141 (MQ=255) aaaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTc < 2:172638/140‑1 (MQ=255) aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGACAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTct < 2:922902/141‑1 (MQ=255) gaagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctata > 2:540498/1‑141 (MQ=255) aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac < 1:132792/141‑1 (MQ=255) aagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac < 1:725227/141‑1 (MQ=255) acagacGTACAACAGATGTAATAGATGCA‑AAAAAATTAACGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCCTTCctataccag < 2:606830/141‑4 (MQ=255) atactttCATAAGTTGAAATTGATGCA‑AAAAAATTATCGCAAACACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat < 1:76545/136‑1 (MQ=255) agacGTTCATCAGTTGTCGTTTCGGTA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat > 1:728498/1‑141 (MQ=255) agacGTTCATCAGTTGTAATTGCTGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAGATGATCCTAGCATGCGCTGTTctatactaaaa > 2:570450/1‑138 (MQ=255) gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAACCTGAAGAACAGTCTTCAGATAAACACAACTAATAACAATGCGCTTGCTAGTACCTCTTAAAAAGATGATGCAAGCATGCGCTTTTCAATACtatata > 2:523919/1‑141 (MQ=255) gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatata > 1:610731/1‑141 (MQ=255) gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAAGATGCTAGCAAGAGCTTTTCTATACaatata > 2:326906/1‑141 (MQ=255) cGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCTCTTTTCTATACTATAtatt < 2:865229/141‑1 (MQ=255) atcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt < 2:370602/141‑1 (MQ=255) tcaGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTAAATATTAttttt > 2:130473/1‑141 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTCTATACTATATATTAttttt > 2:445535/1‑141 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt > 1:82648/1‑141 (MQ=255) tcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCCTTTTCATCCTATATATTAttttt > 2:415523/1‑141 (MQ=255) tcaGTTGTAATTGACGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt > 1:545937/1‑141 (MQ=255) tcaGTTGCAATGGACGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAGCGCTTTTCTATACTATATATTAttttt < 1:245225/141‑1 (MQ=255) caGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGCAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc > 1:634381/1‑141 (MQ=255) caGTTGTAACTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc < 1:75405/141‑1 (MQ=255) caGATGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc < 1:701729/141‑1 (MQ=255) aGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt > 2:908291/1‑141 (MQ=255) aGTTGTAATTGATGCA‑AAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTa > 1:261230/1‑141 (MQ=255) tGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGAAGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaa > 2:447198/1‑141 (MQ=255) gTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaaa < 2:536359/141‑1 (MQ=255) gTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaaa < 1:595765/141‑1 (MQ=255) aaTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt > 2:541461/1‑141 (MQ=255) aaTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt > 2:985902/1‑141 (MQ=255) aTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt < 2:782854/141‑1 (MQ=255) aTTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCGTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt > 2:979964/1‑141 (MQ=255) tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCGTTTCTATACTATAAATTATTTTTCTAAAATTTTa > 2:815949/1‑141 (MQ=255) | AGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGA‑CTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTA > USA300TCH1516_ALE/2455559‑2455826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |