| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,196,508 | C→A | S338Y (TCT→TAT) | ftsZ → | Cell division protein FtsZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,196,508 | 0 | C | A | 99.0% | 301.2 / NA | 97 | S338Y (TCT→TAT) | ftsZ | Cell division protein FtsZ |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (41/54); total (42/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC > USA300TCH1516_ALE/1196370‑1196645 | tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTtata > 2:796322/1‑141 (MQ=255)tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTtata > 2:586509/1‑141 (MQ=255)tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTtata > 1:390707/1‑141 (MQ=255)tGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGAAAATACTtata > 1:461941/1‑141 (MQ=255) aTTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGc > 1:882481/1‑141 (MQ=255) tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACCTGGTAGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaa < 1:678478/141‑1 (MQ=255) tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaa < 1:129908/141‑1 (MQ=255) cGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGTTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAACACTGGAGTCGGAACAAACGAAAATACTTATAGCAATGc > 2:628334/1‑141 (MQ=255) aCAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGTGTAAATACTTATAGCAATGCAACt < 1:721916/141‑1 (MQ=255) aCAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACt < 1:945475/141‑1 (MQ=255) aGTTATTAATCCTAAATTACAAGATGCGATCGCTGCAACAGTTATTCCAACCGGTTTTGATGACACCCAAACATCACATGCTCGCCTGACATGTATCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTc < 1:231957/141‑1 (MQ=255) gTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCt > 1:669778/1‑141 (MQ=255) ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTa > 2:879232/1‑141 (MQ=255) tattaATCCTGAATTAGAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTaa < 1:296653/141‑1 (MQ=255) tattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTTCAACTGGTTTTGATGACAAACCAACATCACATGGGCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTaa < 2:277392/141‑1 (MQ=255) tattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTaa < 1:117677/141‑1 (MQ=255) aaTCCTGATTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCAGATGGTTGTAAATCTGGTATCACTGGAATCGGATCAAGAGTAAATAATTATAGCAAGTCAACTTCTAAAGAt > 2:313845/1‑141 (MQ=255) aaTCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGAt > 1:874265/1‑141 (MQ=255) tCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGa < 1:804152/141‑1 (MQ=255) tCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGa > 2:898256/1‑141 (MQ=255) cTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCACTGCAACTTCTAAAGATGAAt > 2:230845/1‑141 (MQ=255) cTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAAt < 1:702088/141‑1 (MQ=255) tGAATTACAATATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATAACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGTATTCTGCACAAGCGTAAATACCTATAGCAATGCAACTTCTAAAGATGAATc < 1:166289/141‑1 (MQ=255) actACAAGATGAGATTGTTGTAACAATGATTGCAACTGGTTTTGATGACCAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGCACAAGCGTAAATACTTATAGCAATGCAACTTATAAAGATGAATCagt < 2:855762/139‑3 (MQ=255) aTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt < 2:93543/141‑1 (MQ=255) ttACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACAAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATAATTc > 1:58535/1‑141 (MQ=255) tACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCa > 1:453163/1‑141 (MQ=255) tACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAAAAACCATCAAATGGTCGTAAATCTGGTAGAACTAGATTCGGACCAAGCGTAATTACTTAAAGCAAGGCAACTTCGAAAGATGAATCATTcc > 2:192493/1‑140 (MQ=255) aCAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAc < 2:335644/141‑1 (MQ=255) aCAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAACCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAc < 2:399992/141‑1 (MQ=255) aCAAGATGAGATTGTTGTAACAGTTATTGCAACTGGCTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCAc < 1:329462/141‑1 (MQ=255) aGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGCAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCACTCACTTc > 2:837309/1‑141 (MQ=255) gATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑a > 1:883429/1‑141 (MQ=255) tGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGCATCCTTCCCTTC‑aaa > 2:330313/1‑141 (MQ=255) gagaTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAAt < 2:182529/141‑1 (MQ=255) caTTGTTGGAACATTTATTGCAACTGGTTTGGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACCTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATtc < 2:648362/140‑1 (MQ=255) gttggtgGAACAGTTATTGGAACTGTGTGTGATGACAAACCAACAGCACATGGTCGTATAGCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑ATATtca < 2:336274/140‑1 (MQ=255) ttgttgTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATtcat > 1:306420/1‑141 (MQ=255) aCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGca > 2:55433/1‑141 (MQ=255) ttATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAg > 1:454011/1‑141 (MQ=255) ttATTGCAACTGGTTTTGAAGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAg < 1:841503/141‑1 (MQ=255) ttGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCaa > 1:230298/1‑141 (MQ=255) ttGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCaa > 1:932022/1‑141 (MQ=255) gCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACt > 2:889022/1‑141 (MQ=255) aCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGAt > 1:90512/1‑141 (MQ=255) aCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGCAAATAATTATAGCAATGCGACTTCTAAAGATTAATCATTGACTTC‑AAATTCATCAAATGCACAAGCAACTGAt > 2:233057/1‑141 (MQ=255) ggTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATGCATCAAATGCACAAGCAACTGATAgt < 2:195463/141‑1 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAGTCATTCCCTTC‑AAATTCATCAAATGCACAAGCAACTGATAgtt > 1:181887/1‑140 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAgtg > 1:23292/1‑141 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAgtg < 1:793438/141‑1 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAgtg < 2:911651/141‑1 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAgtg > 1:114810/1‑141 (MQ=255) tgatgaCACACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAACACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAg < 2:909504/141‑1 (MQ=255) caCAACCCAACATCACAGGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaa < 1:170025/140‑1 (MQ=255) aCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaaa < 2:882481/141‑1 (MQ=255) aCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaaa > 1:454221/1‑141 (MQ=255) aCAAACCAACATCACATGGTCGTAAATCTGGTAGCAACGGCTTCGAACCAACCGCAAATACTTCTAGCACACCAGTTTACAACCCTGAAGCATGCACGTC‑GATTGCATTAAATGCACAATTAACTGATAGTGTAAGTGaaa > 2:574382/1‑141 (MQ=255) aCAAACCAAAATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGAAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATATTGTAATTTaaa > 2:259830/1‑141 (MQ=255) cAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAg < 1:898256/141‑1 (MQ=255) aaaaCCAACATCACATGGGCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAg < 2:58535/140‑1 (MQ=255) aaCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTACGTGAAAGaa < 2:195866/141‑1 (MQ=255) ccAACATCACATGGTCGTAAATCTTGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAaca < 1:81536/141‑1 (MQ=255) ccAACATCACATGGTCGCAAAGAAGGAATCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAaca > 1:982206/1‑141 (MQ=255) aCATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAATCAACTGATAGTGTAAGTGAAAGAAcacaa > 2:460169/1‑140 (MQ=255) aCATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACAt > 2:336585/1‑141 (MQ=255) aTCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATAc < 2:46332/141‑1 (MQ=255) acaTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATaaaaa > 2:430153/1‑140 (MQ=255) acaTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAAc > 2:529595/1‑141 (MQ=255) acaTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAAc < 1:758242/141‑1 (MQ=255) aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTa < 1:392181/141‑1 (MQ=255) ggTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTaaa > 1:935577/1‑141 (MQ=255) cGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaagaa < 1:879232/141‑1 (MQ=255) gTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACTCATACAACTAaagaag < 1:586509/141‑1 (MQ=255) gTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaagaag < 1:796322/141‑1 (MQ=255) aTCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga < 2:164783/141‑1 (MQ=255) aTCTGGTAGCACTGGATTCCGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga < 1:79349/141‑1 (MQ=255) tgttAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAAAAGATGaaat > 2:499340/4‑141 (MQ=255) tGGTAGCACTGGATTCGGAACCAGCGTAACGCCTTTTCGAGCTGCTTTTCCCGAAGAAGGATCACTTTCTGG‑AAATTCATTAAAGGCACAAGCTGCGGGGAGTGTAGGTGGAAGAACACATACAACTAAAGAAGATGatat < 2:330812/141‑1 (MQ=255) tGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGatat < 2:724768/141‑1 (MQ=255) ggTAGCACTGGAGTCGGAACAAGCGTCAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATAtt < 2:609726/141‑1 (MQ=255) gTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc > 2:362962/1‑141 (MQ=255) aGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCt > 2:295322/1‑141 (MQ=255) cACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAg < 1:326657/141‑1 (MQ=255) aTTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTACGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCAt > 1:923797/1‑141 (MQ=255) aTTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCAt < 1:151197/141‑1 (MQ=255) aTTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCAt < 1:553421/141‑1 (MQ=255) tCGTAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTa < 1:687809/141‑1 (MQ=255) tCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTa < 1:242485/141‑1 (MQ=255) cGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAg < 1:336585/141‑1 (MQ=255) aaGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAAAATCA‑AAAGTGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATag < 1:469880/141‑1 (MQ=255) aaGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATag < 1:795202/141‑1 (MQ=255) gCGTAAATACTTATAGCAATGCAACGTCTAAAGATGAATCATTCACTTC‑AAATTCAGCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATagag < 2:599010/141‑1 (MQ=255) gTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATAAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGagaa > 2:597889/1‑141 (MQ=255) aaaTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGagaaga < 2:263353/141‑1 (MQ=255) tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATCCAACTAAAGAAGATGATATTTCTAGCTTCATTAGAAATAGAGaagaaag > 1:80690/1‑141 (MQ=255) tACTTATAGCAATGCAACTACTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag > 1:543044/1‑141 (MQ=255) ccTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaaga < 2:537226/140‑1 (MQ=255) aCTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaaga < 1:13306/141‑1 (MQ=255) cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc < 1:889022/141‑1 (MQ=255) cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc < 1:321925/141‑1 (MQ=255) | TGATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTC‑AAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC > USA300TCH1516_ALE/1196370‑1196645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |