Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,010,363	C→T	C146Y (TGT→TAT)	USA300HOU_RS10125 ←	Putative multidrug export ATP‑binding/permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,010,363	0	C	T	100.0%	254.1 / NA	87	C146Y (TGT→TAT)	USA300HOU_RS10125	Putative multidrug export ATP‑binding/permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/42);  total (45/42)

CTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGT  >  USA300TCH1516_ALE/2010225‑2010498                                                                                                                                            |                                                                                                                                        ttttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATaa                                                                                                                                       <  2:583907/140‑1 (MQ=255)   ttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCACTTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAAGAATTGTTATATAATc                                                                                                                                     >  2:772111/1‑141 (MQ=255)    tCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCt                                                                                                                                    <  2:667955/141‑1 (MQ=255)    tCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGGGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGTAATAATAATTGTTATATAATCt                                                                                                                                    >  1:683928/1‑141 (MQ=255)      aCGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTaa                                                                                                                                  >  1:965089/1‑141 (MQ=255)      aCGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAAGTTCACATATAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTaa                                                                                                                                  >  1:730510/1‑141 (MQ=255)           tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAGATGGGAAGATAAACAGTGCGGCTAAAGTCAACTTCACATCTAACAAGAAC‑ATTATGGATAGTGCAATAATAATTGTAATATAATATAACCaaa                                                                                                                             >  2:163729/1‑141 (MQ=255)           tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACCGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaaa                                                                                                                             >  1:522527/1‑141 (MQ=255)           tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACaaaa                                                                                                                             >  2:976433/1‑141 (MQ=255)           tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaaa                                                                                                                             >  2:140902/1‑141 (MQ=255)             aaTTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAata                                                                                                                           >  2:491840/1‑141 (MQ=255)                  tCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAt                                                                                                                      <  2:89831/141‑1 (MQ=255)                    ttAATCTTCCAAAGAAAACGTACACCGCTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTa                                                                                                                    >  1:982312/1‑141 (MQ=255)                       aTCTCCCAAAAAAAACGTACACCGTTAAAACGTAAACTGGGTCGCTAACTCGTGCTGCTAAAGTAACTTTAACGCCGATACCGACCGAATCT‑GAGTGTGCAATAATGATTGTTATATAATCTAACCAAATATTCATTAAcc                                                                                                                 <  1:976436/141‑1 (MQ=255)                           cccAAAGAAAACGAACACCGTTAAAATGTAAAAGGGGAAGAAAAACAGGGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGt                                                                                                             <  1:77504/140‑1 (MQ=255)                             cAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTCACCAAATATTCATTAACCCGGTTa                                                                                                           >  2:790424/1‑141 (MQ=255)                             cAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTa                                                                                                           >  2:1661/1‑141 (MQ=255)                               aaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTtaa                                                                                                         >  1:531939/1‑141 (MQ=255)                               aaGAAAACGTACACCATTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAACCGGTTaaa                                                                                                         >  1:734279/1‑141 (MQ=255)                                  aaaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                      <  2:556888/141‑1 (MQ=255)                                  aaaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                      <  1:376657/141‑1 (MQ=255)                                  aaaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                      <  2:166226/141‑1 (MQ=255)                                      cGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAt                                                                                                  >  2:16544/1‑141 (MQ=255)                                      cGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAt                                                                                                  <  1:795420/141‑1 (MQ=255)                                      cGTACACCGGTAAAGTGTAAAAGGGGAAGAGAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAt                                                                                                  >  2:870946/1‑141 (MQ=255)                                       gTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATc                                                                                                 <  1:915837/141‑1 (MQ=255)                                        tACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTCACCCGGTTAAAATGAAATCt                                                                                                >  2:747552/1‑141 (MQ=255)                                        tACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGGGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAtat                                                                                                >  2:231804/1‑139 (MQ=255)                                             cGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATTAAATTTtttgt                                                                                           >  2:13702/1‑141 (MQ=255)                                             cGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAAACTAACCAAATATTCATTAACCCGGTTAAAATCAAATCTtttgt                                                                                           >  2:219778/1‑141 (MQ=255)                                             cGTTAAAATGGAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTACCCCGGTTAAAATGAAATCTtttgt                                                                                           <  1:74859/141‑1 (MQ=255)                                                  aaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTtgtttgtt                                                                                      <  1:247300/141‑1 (MQ=255)                                                   aatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc                                                                                     <  2:84391/141‑1 (MQ=255)                                                    aggtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAAGTGTTATATAATCTAACCAAATATTCACTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCa                                                                                    <  1:876527/139‑1 (MQ=255)                                                     tgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCaa                                                                                   >  2:634510/1‑141 (MQ=255)                                                      gcagatggggAAATTAAACAGTTCTGCGAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAc                                                                                  <  2:392664/134‑1 (MQ=255)                                                       taaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAG‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCCTTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAca                                                                                 >  2:280234/1‑141 (MQ=255)                                                       taaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTCAAATGAAATCTTTTGTTTGTTCAAca                                                                                 >  2:272704/1‑141 (MQ=255)                                                       taaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAca                                                                                 >  1:598404/1‑141 (MQ=255)                                                       taaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAca                                                                                 >  2:466825/1‑141 (MQ=255)                                                       taaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATAGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAca                                                                                 >  2:7712/1‑141 (MQ=255)                                                           atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                             <  2:130795/141‑1 (MQ=255)                                                           atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                             >  2:923514/1‑141 (MQ=255)                                                           atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAACCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                             >  2:891507/1‑141 (MQ=255)                                                               gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACTCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTact                                                                         >  2:310308/1‑139 (MQ=255)                                                               gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                         >  2:501532/1‑141 (MQ=255)                                                                   aTAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCaac                                                                     >  1:752219/1‑139 (MQ=255)                                                                        cAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGa                                                                >  1:941275/1‑141 (MQ=255)                                                                         aGTGCTGCTAAAGCCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGat                                                               <  1:865673/141‑1 (MQ=255)                                                                            gctgctAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTATCAGAATAAAAAAATACACTTTTGTTTGTTCAACATTATTAATCACTCTAGATAtt                                                            >  1:714746/1‑141 (MQ=255)                                                                            gctgctAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATAtt                                                            >  1:207413/1‑141 (MQ=255)                                                                            gctgctAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATAtt                                                            <  2:231890/141‑1 (MQ=255)                                                                            gctgctAAAGTCAATTTCACAGTTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATAtt                                                            <  2:214236/141‑1 (MQ=255)                                                                             ctgctAAAGTCAATTTCATATCTACAAAGACT‑ATTCTGGATTGTGTAACAATAATTGTTATATAATCTATCAAAACAGACATCCTCCGAGTCGAAACGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTa                                                           <  1:976433/141‑1 (MQ=255)                                                                               gctAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACt                                                         >  1:385124/1‑141 (MQ=255)                                                                                        aaTTTCGCATCACAAAAGAAC‑ATTATGGATAGTGCAATAATAGTTGTTATATGATCTAACCAAATATTCGTTAACGCGGTTAAAATGAAATCTTTTGTTTTTTCAACATAATTAGTCACTCTACATATTACTGGACCGACt                                                >  2:182496/1‑141 (MQ=255)                                                                                         aTTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACtt                                               >  2:782308/1‑141 (MQ=255)                                                                                         aTTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACtt                                               <  1:1661/141‑1 (MQ=255)                                                                                          tttCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTg                                              <  1:562871/141‑1 (MQ=255)                                                                                            tCACTTCTAAAAAGAAC‑ATTATGTATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGat                                            <  2:432673/141‑1 (MQ=255)                                                                                            tCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGCTAAAACGAAATCATTTCTTAGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGat                                            <  2:577545/141‑1 (MQ=255)                                                                                              acaTCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattt                                          >  2:809113/1‑140 (MQ=255)                                                                                               caTCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACAAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCAACTTGattat                                         >  1:66596/1‑141 (MQ=255)                                                                                                 tCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACGCTAGATATTACTTGACCTACTTGAttatta                                       >  1:182121/1‑141 (MQ=255)                                                                                                     aaaaGAAC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGAtttttacctt                                   >  2:179741/1‑137 (MQ=255)                                                                                                         aaaC‑ATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATaaaa                               <  2:805785/140‑1 (MQ=255)                                                                                                             ccgtgtGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtct                            >  2:507714/6‑141 (MQ=255)                                                                                                                 aTGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACATACTTGCTTATTAGCATACAATCTCGCa                        >  2:899458/1‑141 (MQ=255)                                                                                                                 aTGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTCTTGTTTGTTCAACATCATATATCATCCCAACTATTACTTGACGTTCTTGATTATTAGCATAAAATCTCGCa                        >  1:967924/1‑141 (MQ=255)                                                                                                                   ggATAGTGCAATAATAATTGTTATATAATCTATCCTAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACt                      <  2:916198/141‑1 (MQ=255)                                                                                                                    gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTTACCTACTTGATTATTAGCATAAAATCTCGCACtt                     <  1:124480/141‑1 (MQ=255)                                                                                                                    gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACtt                     <  1:498391/141‑1 (MQ=255)                                                                                                                     aTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAGAATGAAATCTTTTGTTTGTTCAACATCATTAATCATTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACtga                    <  2:730510/141‑3 (MQ=255)                                                                                                                     aTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTa                    <  2:965089/141‑1 (MQ=255)                                                                                                                         tGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCTCTCTAGAGATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGc                <  1:140521/141‑1 (MQ=255)                                                                                                                         tGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTATATATTACTTGACCTACTTGATTATTAGCATCAAATCTCGCACTTAAAGc                <  2:498112/141‑1 (MQ=255)                                                                                                                         tGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGc                <  2:940438/141‑1 (MQ=255)                                                                                                                         tGCAAGAAGAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAAGGAAATCTTTTTTTTGTTCAACATCATTAAGCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGc                <  1:690615/141‑1 (MQ=255)                                                                                                                            aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg             <  2:776953/141‑1 (MQ=255)                                                                                                                             ataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGt            <  2:428098/141‑1 (MQ=255)                                                                                                                             ataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGt            >  1:12052/1‑141 (MQ=255)                                                                                                                             ataataatTGTTATATAATCTAACCAAATATTCAGTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGAGTATTAGCATAAAATCTCGCACTTAAAGCTTGt            <  2:927463/141‑1 (MQ=255)                                                                                                                               aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaa          <  1:46358/141‑1 (MQ=255)                                                                                                                               aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaa          <  1:931563/141‑1 (MQ=255)                                                                                                                                    ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt     <  1:140902/141‑1 (MQ=255)                                                                                                                                    ttGTTATATAAGCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt     <  1:163729/141‑1 (MQ=255)                                                                                                                                       ttATATCATCTAACCAAATACTCATTAATCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATCATGTCTCGCACTTAAAGCTTGTAAATGGTTGt  <  1:283923/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        CTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAAC‑ATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGT  >  USA300TCH1516_ALE/2010225‑2010498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑