Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,709,609	C→A	18.6%	E440* (GAG→TAG)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,709,609	0	C	A	18.6%	128.8 / 13.6	61	E440* (GAG→TAG)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand):  ref base C (26/23);  new base A (8/4);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCACTGAGC  >  USA300TCH1516_ALE/2709471‑2709745                                                                                                                                           |                                                                                                                                         caATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCAAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCctct                                                                                                                                         >  1:467736/1‑140 (MQ=255) caATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAAACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCctct                                                                                                                                         >  1:87123/1‑140 (MQ=255)  aaTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATCACTTTTTAATATCCTCtt                                                                                                                                        >  2:348481/1‑140 (MQ=255)   aTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAGGGCATTACCAATATCCACCATGATCGAGTCACCAATTAATAGTAGTAATAACTTTTTAATATCCTtttt                                                                                                                                       >  1:127700/1‑140 (MQ=255)       gtgtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTa                                                                                                                                   >  1:537244/1‑140 (MQ=255)              aTCAACGAGTTGCCGTCCAACTTTACCATCATTTTGTGCATTTGGTATTTTTTTAGTTAAGACATTTCCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTg                                                                                                                            <  1:42417/140‑1 (MQ=255)                    gAGTTGCCGTCCAACTTTACCATAAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTGCCCAATTAATAGTGGTGATTACTTTTTAATTTCCTATTTGCTATTCGCTGTCTGCt                                                                                                                      >  2:47889/1‑140 (MQ=255)                     aGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCCTTGCTATTCGCTGTCTGCtt                                                                                                                     >  2:185323/1‑140 (MQ=255)                        tGCCGCCTAACTTTCCCATCATTTTGTGCATTTCGTATTGTCTTAGTAATGTCATTACCAATGTCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTAGTGGCTATTCGCTGTCTGCTTATc                                                                                                                  <  2:377726/140‑1 (MQ=255)                         gCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATcc                                                                                                                 >  1:290353/1‑140 (MQ=255)                          ccGTACAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGGGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTGATCct                                                                                                                >  1:374765/1‑140 (MQ=255)                               cAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTga                                                                                                           >  1:502652/1‑139 (MQ=255)                                     tACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCt                                                                                                     <  1:540264/140‑1 (MQ=255)                                     tACCAACAATTTGTGTATTTGGTATTTTCTTAGTAAAGACAATACCTATATCCACCATGATCGAGTCTCCATTTAATAGTGGTGATGCCTTTTTAATATCCTCTTTGCTATACGCTGTATGCTTATTCTCTTGCTTATCt                                                                                                     <  2:486112/140‑1 (MQ=255)                                      aCCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACGAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCtt                                                                                                    >  2:293560/1‑140 (MQ=255)                                       ccATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCttt                                                                                                   >  2:463658/1‑140 (MQ=255)                                           cAATTTGTTCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                                               <  1:141192/140‑1 (MQ=255)                                                tgtgCATTTGGTATTTTCTTAGTAAACAAACGACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCCTTGCTATTCGCTGTCTACATATCCTCTTGCTATTCTTTTTTAACGAc                                                                                          >  1:543470/1‑140 (MQ=255)                                                  tgCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCGTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACtt                                                                                        >  2:143378/1‑140 (MQ=255)                                                                   tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTCATTTGGGTTAACACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAATGACTTTTTTCTTCGTTGttttt                                                                       >  2:472174/1‑139 (MQ=255)                                                                   tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAATGACTTTTTTCTTCGTGGTTCTa                                                                       >  2:326859/1‑140 (MQ=255)                                                                   tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTTATCCACTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTa                                                                       >  1:167912/1‑140 (MQ=255)                                                                   tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATACTCTTTACTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTa                                                                       >  1:181438/1‑140 (MQ=255)                                                                     gTAAATACATTCCCAATTTCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTaaa                                                                     <  2:178810/140‑1 (MQ=255)                                                                          gACATTACCAATATCCACCATGGCCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCg                                                                <  2:207839/140‑1 (MQ=255)                                                                                 ccAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCATTGTTTTAAATTCCGtcttctt                                                         >  1:396387/1‑140 (MQ=255)                                                                                 ccAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGAC‑TTTTTCTTCGTTGTTTTAAATTCCGtcttcttc                                                        >  2:180960/1‑140 (MQ=255)                                                                                 ccAATATCCACCATGACCGAGTCACCAATTAATAGTGACGATGACTTTTTAATATCCTCATTGCTAATCGCTGTCTGCTTATCCACATGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                                         >  1:13942/1‑140 (MQ=255)                                                                                  cAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACATTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTCTCTTTTTTCATTTTTTTTTTCTTAGTTGTTTGAGCTTACGtcttcttc                                                        >  2:329646/1‑140 (MQ=255)                                                                                         cactaTGACCGAGTCATCGATTAATAGTGGTGATTTCTTGTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACCACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtctttct                                                 <  1:317703/140‑1 (MQ=255)                                                                                         caccaTTACCGAGTCACCAATCAATAGTGGTGATGACTTTTTAATATCCTCTTTCCTATTCGCTGTCTTCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtctttct                                                 <  2:480515/140‑1 (MQ=255)                                                                                         caccaTGACCTAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtctttct                                                 <  2:3234/140‑1 (MQ=255)                                                                                         caccaTGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtctttct                                                 <  2:380102/140‑1 (MQ=255)                                                                                          accaTGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtctttctt                                                >  1:326501/1‑140 (MQ=255)                                                                                          accaTGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTGAAATTCCGTCTTCTTCtctttctt                                                <  1:350596/140‑1 (MQ=255)                                                                                           ccaTGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTTTttcttc                                               >  2:417543/1‑140 (MQ=255)                                                                                                aCCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTTTTCGTTGTTTTAAATTGCGTATTCTTCTCTTTTTTCTCGGc                                          >  2:125770/1‑140 (MQ=255)                                                                                                aCCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTTTTCTTCTCTTTCTTCTCGGc                                          >  2:352192/1‑140 (MQ=255)                                                                                                 ccGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCTGCTTCTTCtctttctttttgtct                                         >  2:270282/1‑133 (MQ=255)                                                                                                 ccGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCt                                         <  1:53636/140‑1 (MQ=255)                                                                                                   gAGTGACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTTACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTc                                       <  2:82638/140‑1 (MQ=255)                                                                                                   gAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTc                                       <  2:411653/140‑1 (MQ=255)                                                                                                   gAGGCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTc                                       <  1:478237/140‑1 (MQ=255)                                                                                                        aCCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgtt                                  >  2:219691/1‑140 (MQ=255)                                                                                                        aCCAATTAATAGTGGTGATGACTTTTTAATATCCTATTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgtt                                  <  2:537244/140‑1 (MQ=255)                                                                                                         ccAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg                                 <  1:371735/140‑1 (MQ=255)                                                                                                             ttAATAGTGGTGATGACTTTTTAGGATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTGTGTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTg                             <  1:531569/140‑1 (MQ=255)                                                                                                             tgaaTAGTGGTGAGGACTGTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGCCTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTg                             <  2:104242/138‑1 (MQ=255)                                                                                                               aaTAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGcc                           <  1:188113/140‑1 (MQ=255)                                                                                                               aaTAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTGCTCGGCTTCATGTTTTTTGcc                           >  2:550090/1‑140 (MQ=255)                                                                                                                  aGTGGTGATGACTTTTTAATTTCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAg                        <  1:32372/140‑1 (MQ=255)                                                                                                                   gtggtgATTACTTTTTACTATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt                       <  2:168955/140‑1 (MQ=255)                                                                                                                      aggatgaCTTTTTAATATCCTCCTTTCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCa                    <  2:106762/138‑1 (MQ=255)                                                                                                                       tgatgaCTTTTTTATATCCTATTTGCTTTTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAGCGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAt                   <  2:542749/140‑1 (MQ=255)                                                                                                                        gatgaCTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGAATc                  >  1:498048/1‑140 (MQ=255)                                                                                                                            aCTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAAc              >  2:98742/1‑140 (MQ=255)                                                                                                                            aCTTTTTAATATCCTCTTTGCTATTCGCTGACTGCTAAAACTCATGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAAc              >  1:66349/1‑140 (MQ=255)                                                                                                                             cTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACt             >  1:403351/1‑140 (MQ=255)                                                                                                                             cTTTTTAATATCCTCTTTGCTATTCGCTATCTGCTTATCCTCTTTCTTATCTTTTTTACCGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCACGGCTTCATGTTGTTTGCGAATTGCATCACACt             >  1:334054/1‑140 (MQ=255)                                                                                                                                  tAATATCCTATTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCa        >  1:175754/1‑140 (MQ=255)                                                                                                                                   aaTATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCAc       <  1:354174/140‑1 (MQ=255)                                                                                                                                    gtatCCTCTTTGCTATTAGGTGTCTGCTTATCATCTTGCTTATATTTTTTAAAGAATTTTTTGTTCGTTGTTTTAAATTCTATCTTCTTTTCTTTATTTTGGGCTTCATGTTGTTTGCCATGTGAATCAAACTggctcct      >  2:69091/2‑134 (MQ=255)                                                                                                                                     tatCCTATTTGCTATTCGCTGTCTGCTTATCCTAATGCTTAACTTTTGTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCACTg     <  1:372851/140‑1 (MQ=255)                                                                                                                                        ccTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCACTGAGc  >  1:429581/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                         CAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAACTGTCCACTGAGC  >  USA300TCH1516_ALE/2709471‑2709745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑