Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5160CA25.9% 231.6 / 71.3 131Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (53/44);  new base A (19/15);  total (72/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

GAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAT  >  USA300TCH1516_ALE/111381‑111652
                                                                                                                                       |                                                                                                                                          
gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAAATTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat‑a                                                                                                                                       <  1:775424/140‑1 (MQ=255)
 aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat‑at                                                                                                                                      >  2:131400/1‑140 (MQ=255)
 aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat‑at                                                                                                                                      >  1:888009/1‑140 (MQ=255)
   gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCTCTATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCa                                                                                                                                    >  2:329693/1‑140 (MQ=18)
   gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCa                                                                                                                                    >  2:464793/1‑140 (MQ=255)
      aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGCGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAAtt                                                                                                                                 <  2:211984/140‑1 (MQ=255)
      aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAAtt                                                                                                                                 >  1:898260/1‑140 (MQ=255)
      aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAAtt                                                                                                                                 >  2:401284/1‑140 (MQ=255)
      aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAAtt                                                                                                                                 >  1:179308/1‑140 (MQ=255)
      aaaGAAGAAAACATAAAAAAAGAAATCGAAAACATTAAGTTGTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGATAAAT‑ATCAAtt                                                                                                                                 >  2:917472/1‑140 (MQ=18)
       aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTa                                                                                                                                >  1:857374/1‑140 (MQ=255)
       aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTa                                                                                                                                >  1:772620/1‑140 (MQ=255)
       aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTa                                                                                                                                <  2:137627/140‑1 (MQ=255)
          aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACt                                                                                                                             <  2:810640/140‑1 (MQ=255)
           agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTTTTTTTTTTTCAATATAAAAGGTTTAATGAATTAAAAAGATGGAGATAGATTATATAATTTAGGGGTTCCGAGTTATTAAGATCAAT‑AAAAATTAAATa                                                                                                                            >  2:391415/1‑140 (MQ=12)
           aaaaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTACAT‑AGCAATTAACTa                                                                                                                            <  2:681793/138‑1 (MQ=18)
            gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTaa                                                                                                                           >  1:839843/1‑140 (MQ=255)
            gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACAAT‑ATCAATTAACTaa                                                                                                                           >  2:721556/1‑140 (MQ=255)
            gaaAACATAAAAAAAGAAATCAAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTaa                                                                                                                           <  2:958040/140‑1 (MQ=255)
             aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATCGCAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAAACCAGAGGTGGCGTGTTATTCAGCTCTAT‑ATCAATTAACTAat                                                                                                                          >  2:643017/1‑140 (MQ=18)
             aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAat                                                                                                                          <  1:352096/140‑1 (MQ=255)
             aaaaCATAAAAAAAGAAAACGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATACATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAat                                                                                                                          <  1:457379/140‑1 (MQ=255)
               aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGCTTTAATGAATTATAAAGATGGAGATATATCATATAATCTAACGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAatga                                                                                                                        >  1:222410/1‑140 (MQ=18)
                  aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAAtgatga                                                                                                                     >  2:835490/1‑140 (MQ=255)
                   tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATgataat                                                                                                                    >  1:359010/1‑140 (MQ=255)
                    aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGAtt                                                                                                                   <  1:758888/140‑1 (MQ=255)
                     aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATta                                                                                                                  >  1:142287/1‑140 (MQ=255)
                     aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATta                                                                                                                  <  1:979940/140‑1 (MQ=255)
                      aaaaaGAAATCGAAAACTTTAAGTGTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATGATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATtat                                                                                                                 <  1:675342/140‑1 (MQ=255)
                       gaaaGAAATCGAAAACTTCAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATtata                                                                                                                <  1:54958/139‑1 (MQ=255)
                       aaaaGAAATCGAAAACTTTATGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATtata                                                                                                                <  1:845457/140‑1 (MQ=255)
                       aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATtata                                                                                                                <  2:770960/140‑1 (MQ=255)
                       aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATtata                                                                                                                <  1:221322/140‑1 (MQ=255)
                       aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATtata                                                                                                                <  2:188401/140‑1 (MQ=255)
                        aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATaa                                                                                                               >  2:859699/1‑140 (MQ=255)
                        aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATCGAGATATATCATATAATCCAGAGGGGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATaa                                                                                                               >  1:38337/1‑140 (MQ=255)
                          aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATg                                                                                                             <  1:24611/140‑1 (MQ=255)
                           gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGt                                                                                                            >  1:933098/1‑140 (MQ=255)
                             aatgtaaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTaa                                                                                                          <  1:267861/135‑1 (MQ=255)
                             aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTaa                                                                                                          <  1:270778/140‑1 (MQ=255)
                             aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaa                                                                                                          >  1:547032/1‑140 (MQ=255)
                             aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaa                                                                                                          <  1:817383/140‑1 (MQ=255)
                             aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaa                                                                                                          <  1:42253/140‑1 (MQ=255)
                              ggcGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaaa                                                                                                         <  2:746456/138‑1 (MQ=255)
                              aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaaa                                                                                                         >  1:840821/1‑140 (MQ=255)
                              aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTaaa                                                                                                         <  1:246785/140‑1 (MQ=255)
                                  atgtctgtAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                                                     <  2:493144/133‑1 (MQ=18)
                                  aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                                                     <  1:975401/140‑1 (MQ=255)
                                   aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                    <  1:1005946/140‑1 (MQ=255)
                                    aaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAACACAAtt                                                                                                   >  1:90963/1‑140 (MQ=255)
                                       attAATTTTTTTGCGAAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                                <  1:292094/139‑1 (MQ=255)
                                         tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTAGAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                              <  2:447279/140‑1 (MQ=255)
                                          aaGTTTTTTTTGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                             >  2:880828/1‑140 (MQ=255)
                                          aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTGATCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                             >  1:229704/1‑140 (MQ=255)
                                           aGTTTTTTGCGCAAGATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                            >  1:552409/1‑140 (MQ=17)
                                            gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaaa                                                                                           <  2:239993/140‑1 (MQ=255)
                                             ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAATAATTACGTAAAAg                                                                                          <  2:463914/140‑1 (MQ=255)
                                             ttttgtGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                                                          <  1:98462/140‑1 (MQ=17)
                                             atttttGCGCAATATAGCAATTTTACAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAAT‑ATCAATTAACTAATTATGATTATAATGTAAAACAATGACCCAAAAg                                                                                          <  1:737783/139‑1 (MQ=17)
                                                  tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTACTGTTGATTATAATGTAACACAATTCCGTAAAAGATATg                                                                                     >  1:279820/1‑140 (MQ=255)
                                                  tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTTTAACTATTACGTAAAAGATATg                                                                                     <  1:487308/140‑1 (MQ=255)
                                                    cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAc                                                                                   >  2:878437/1‑140 (MQ=255)
                                                      cAATCTCGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                 <  1:112714/140‑1 (MQ=255)
                                                        aattaGCAATGTTAAAGTTTTAATGAATAAAAAAGATGGAGAGATATCATATAATCCAGAGGTTCCCAGTTATTCCGCTCAAT‑ATCAATTCACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAc                                                                               <  2:27354/137‑1 (MQ=17)
                                                                      aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                 >  2:934834/1‑140 (MQ=255)
                                                                      aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                 <  2:839580/140‑1 (MQ=255)
                                                                      aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                 <  2:777681/140‑1 (MQ=255)
                                                                      aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGACATGACATACCAACAAATAAAgcg                                                                 >  2:152359/1‑140 (MQ=255)
                                                                          tttAATGAATTATAAAGTGGGAGATATATCATATAATCCAGAGGTGCCGAG‑TAACAAGCAATATGTTCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGa                                                            <  1:477737/140‑1 (MQ=17)
                                                                                gAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACAAAAAAATAAAGAC‑CCGAAGCagt                                                      >  2:180068/1‑140 (MQ=17)
                                                                                 aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAGAAAGCG‑CCGAAGCagta                                                     >  1:424629/1‑139 (MQ=255)
                                                                                    cagaaaGATGGAGATATATCATATTATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTgttgtt                                                  <  2:245937/137‑1 (MQ=255)
                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGa                                                >  2:329037/1‑140 (MQ=255)
                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAAATAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGa                                                >  2:442399/1‑140 (MQ=255)
                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGaaa                                              >  2:773909/1‑140 (MQ=255)
                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGaaa                                              >  1:587522/1‑140 (MQ=255)
                                                                                         aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAg                                             >  2:336534/1‑140 (MQ=255)
                                                                                         aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAg                                             >  2:543679/1‑140 (MQ=255)
                                                                                               agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAGTAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAg                                       >  1:681219/1‑140 (MQ=255)
                                                                                                 atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAggg                                     >  2:676490/1‑140 (MQ=255)
                                                                                                 atatatCATATAATCCAGAGGTGCCGAGTTATGCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTATAggg                                     <  1:682922/140‑1 (MQ=255)
                                                                                                   atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAAATCATGATGATTGTAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGaa                                   >  1:890020/1‑140 (MQ=255)
                                                                                                      tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGAGAAtgg                                >  1:931558/1‑138 (MQ=255)
                                                                                                             aTCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCGGTTGTTGGAAGGTACAGGGAATTTAAAAGGt                         >  1:824125/1‑140 (MQ=255)
                                                                                                                catagGTGCTGAGTTATTCAGATCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAGAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                      <  2:420981/137‑1 (MQ=255)
                                                                                                                cagtagTGCCCAGTTATTCAGCTCATT‑ATCAACTACTCAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                      <  1:453566/135‑1 (MQ=17)
                                                                                                                  gagGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAAGTtcatc                    >  1:49836/1‑140 (MQ=255)
                                                                                                                      tGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAACAAATGACGTAAAAGATATGACATACCAACAAATAAAGCG‑CAGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                >  1:835255/1‑140 (MQ=255)
                                                                                                                      tGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGCTGGTGCGGTTTAAAGGTACAGGGAATTTAAAAGGTTCATAAGtt                >  1:431452/1‑140 (MQ=14)
                                                                                                                      tGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                >  1:46617/1‑140 (MQ=255)
                                                                                                                      tGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                >  1:715718/1‑140 (MQ=255)
                                                                                                                      tGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGGAAAAGATATGACATACCAAAAAATAAAGAG‑CCGAAGCTGTTGTTGAAAAGTACAGAGAATTTAAAAGGATCATCAgat                >  2:570390/1‑138 (MQ=17)
                                                                                                                        ccGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTCAAACACTTACGTAAAAGATATGACATATCAA‑AAATTAAGAGTCCAAAGCAGGTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg              >  2:963700/1‑140 (MQ=17)
                                                                                                                        ccGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg              >  2:771927/1‑140 (MQ=255)
                                                                                                                         cGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgaa             >  2:955826/1‑138 (MQ=255)
                                                                                                                         cGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa             >  2:732204/1‑140 (MQ=255)
                                                                                                                         cGAGGTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACACTTCCGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATATAAAAGGTTCATCAGTTGGa             >  1:38359/1‑140 (MQ=255)
                                                                                                                               aTTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa       <  2:147201/140‑1 (MQ=255)
                                                                                                                                ttCAGCTCAAT‑ATCAATTAACTAATGATTATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa      <  2:94265/140‑1 (MQ=255)
                                                                                                                                    gCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCAAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaag  >  1:766766/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                          
GAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAAT‑ATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCG‑CCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAT  >  USA300TCH1516_ALE/111381‑111652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑