Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,362	G→A	H104Y (CAC→TAC)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,362	0	G	A	100.0%	279.5 / NA	88	H104Y (CAC→TAC)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/45);  total (43/45)

AATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCA  >  USA300TCH1516_ALE/1744228‑1744498                                                                                                                                       |                                                                                                                                         aattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTg                                                                                                                                     >  1:13817/1‑140 (MQ=255) aattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACAACTAAGGTTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAGTTATGACAGGTTTACCTGGTTTAATTGCATCTTTGTACGTTg                                                                                                                                     >  1:836268/1‑140 (MQ=255)   ttATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                                                                   >  2:391449/1‑140 (MQ=255)     ataAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaa                                                                                                                                 <  1:882747/140‑1 (MQ=255)      taAAGGCAATACATACTACGATACCGCCTAATTTTTCAACTAATTTAATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAAAACACTATGACCTGGTTTAATTGCATCTGTGTACATTGTTaaa                                                                                                                                >  1:708733/1‑140 (MQ=255)        aaTGCAATACCCACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAAc                                                                                                                              <  2:826257/140‑1 (MQ=255)         aTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACa                                                                                                                             >  2:615593/1‑140 (MQ=255)          tGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAACTGCATCTTTGTACATTGTTAAAACAt                                                                                                                            <  2:752427/140‑1 (MQ=255)           gCAATACCTACGACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAACTCATCTGTCATTAACACACGTTGACCTGGTTTAATTTCATCTTTGTACATTGTTAAAACAtt                                                                                                                           <  2:737887/140‑1 (MQ=255)            cAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAttt                                                                                                                          >  1:814928/1‑140 (MQ=255)              aTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                        <  1:350761/140‑1 (MQ=255)                 ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTATCACACGTTGACCTGGTTTAATTGTATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                     <  1:668454/140‑1 (MQ=255)                 ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCGGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                     <  1:591158/140‑1 (MQ=255)                 ccTACTACGATACCGCCGAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                     <  1:365242/140‑1 (MQ=255)                  ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCa                                                                                                                    >  2:872739/1‑140 (MQ=255)                  ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCa                                                                                                                    >  1:434592/1‑140 (MQ=255)                   tactacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCat                                                                                                                   >  2:484895/1‑140 (MQ=255)                    actacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCata                                                                                                                  <  2:432518/140‑1 (MQ=255)                    actacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCata                                                                                                                  <  1:88955/140‑1 (MQ=255)                     ctacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCatat                                                                                                                 >  1:657653/1‑140 (MQ=255)                     ctacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCatat                                                                                                                 <  1:228428/140‑1 (MQ=255)                        cGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                                                                              >  1:799928/1‑140 (MQ=255)                            aCCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGt                                                                                                          >  1:377505/1‑140 (MQ=255)                             ccGCCTAATTTTTCAACTAACTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAGATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTc                                                                                                         >  1:613405/1‑140 (MQ=255)                              cGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTAAAACCAGGAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCa                                                                                                        >  1:689343/1‑140 (MQ=255)                               gCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCAt                                                                                                       >  1:585569/1‑140 (MQ=255)                                ccTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATa                                                                                                      <  2:884831/140‑1 (MQ=255)                                           aaCTAATTTTGTTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                                                                                           >  2:780781/1‑140 (MQ=255)                                             cTAATTTTATTGCTTCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATg                                                                                         <  2:276155/140‑1 (MQ=255)                                              tAATTTTATTGCTGCTTCAAGCGTACCACCATTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGa                                                                                        <  1:591264/140‑1 (MQ=255)                                                  tttATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTc                                                                                    <  2:814928/140‑1 (MQ=255)                                                  tttATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTCTTAAAACATTTGTACCATATTCTAGCTCATACTCCTAACGAATCACtcc                                                                                    >  2:198907/1‑138 (MQ=255)                                                     aTTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACg                                                                                 <  1:975447/140‑1 (MQ=255)                                                      ttGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCActa                                                                                >  2:936690/1‑138 (MQ=255)                                                       tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCAAAACCAATGACTTCACAAg                                                                               >  1:120407/1‑140 (MQ=255)                                                             ttCAATCGTACCACCAGTAGCTAATAAATCCTCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCCAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                         <  1:456247/140‑1 (MQ=255)                                                             ttCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                         <  1:217388/140‑1 (MQ=255)                                                                 aTCGTACCACCAGTAGCTAATAATTCATCTGTAATTAACACAGGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                     <  1:342816/140‑1 (MQ=255)                                                                   cGTACCACCAGTAGCTAATAAATCGGCTGTAATTAGCACACGTTTGCATGGTCTAGTTGGATCTTTGTACATTCTTAAAACATTTGTACCGAGTTCTAGGTCATACTCATAACGGATGAGTTCGAAAGGTGATTTCCctt                                                                   >  2:270167/1‑140 (MQ=255)                                                                   cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACCCGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCctt                                                                   <  1:33387/140‑1 (MQ=255)                                                                   cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCctt                                                                   >  1:833663/1‑140 (MQ=255)                                                                      accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCCTAACGAATTACTTCCCGAGGTAATTTCCcttctt                                                                >  1:221108/1‑140 (MQ=255)                                                                      accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGTGGTAATTTCCcttctt                                                                >  1:65876/1‑140 (MQ=255)                                                                        caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                              >  1:40209/1‑140 (MQ=255)                                                                          ccaGTAGCTCATAAATCATCTGTAATTAATACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCt                                                            <  1:393003/140‑1 (MQ=255)                                                                          ccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCt                                                            <  2:619909/140‑1 (MQ=255)                                                                             gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAc                                                         <  1:357502/140‑1 (MQ=255)                                                                             gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCATTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTCATTTCCCTTCTTTTCTAAc                                                         <  1:562540/140‑1 (MQ=255)                                                                              tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATCTTCCAGGTCATACTCATAACCAATGACCTCACGACGTAATTTCCCTCCTTTTCTAACa                                                        <  2:995334/140‑1 (MQ=255)                                                                              tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACa                                                        <  1:99012/140‑1 (MQ=255)                                                                               aGCTAAAAAATCATCTGTAATTAACGCACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAg                                                       <  2:212789/140‑1 (MQ=255)                                                                                 cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTATTTTCCCTTCTTTTTTACCAGGt                                                     >  1:199936/1‑140 (MQ=255)                                                                                      aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTCAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCaaa                                                <  2:661730/140‑1 (MQ=255)                                                                                         tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGcc                                             <  1:327986/140‑1 (MQ=255)                                                                                          catcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                            <  1:780781/140‑1 (MQ=255)                                                                                           atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAACGCCaa                                           >  1:123467/1‑140 (MQ=255)                                                                                           atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCaa                                           >  1:467097/1‑140 (MQ=255)                                                                                           atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCaa                                           >  1:555384/1‑140 (MQ=255)                                                                                           atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCCTTTCTAACAGGTGCAAAGCCaa                                           >  2:105521/1‑140 (MQ=255)                                                                                            tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAt                                          >  2:736799/1‑140 (MQ=255)                                                                                             cggTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATc                                         >  2:763797/3‑140 (MQ=255)                                                                                               gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATccc                                       >  2:648320/1‑140 (MQ=255)                                                                                                tAATTAACCCTCGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATcccc                                      <  1:90591/140‑1 (MQ=255)                                                                                                tAATTAACATACTTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATcccc                                      <  2:334078/140‑1 (MQ=255)                                                                                                tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAACGCAAAAcccc                                      >  2:437738/1‑140 (MQ=255)                                                                                                tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATcccc                                      <  2:743854/140‑1 (MQ=255)                                                                                                  aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGGACATTGTTAAAACATATGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCAt                                    <  1:14726/140‑1 (MQ=255)                                                                                                   ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCAtt                                   >  2:744930/1‑140 (MQ=255)                                                                                                   ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATGTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCACAGCCAAACCCCAtt                                   >  2:658396/1‑140 (MQ=255)                                                                                                     aaCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAGTTTCCCTTCTTTTCTATCAGGTGCTAATCCCCTCCCCTTTgc                                 >  1:92823/1‑139 (MQ=255)                                                                                                     aaCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCAAGAGGTAATTTCCCTTCTTTTCTAACAGGTACAAAGCCAATCCCAAttta                                 >  1:186293/1‑138 (MQ=255)                                                                                                      acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                >  1:125947/1‑140 (MQ=255)                                                                                                      acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                <  1:992374/140‑1 (MQ=255)                                                                                                       cacacGTTGACCTGGTTTAATTGCATCTTTGTAGATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               >  2:663207/1‑140 (MQ=255)                                                                                                       cacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               <  1:87846/140‑1 (MQ=255)                                                                                                       cacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               >  1:411088/1‑140 (MQ=255)                                                                                                           cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCCAGAGATTGATGCCGAGCGTTTCTAACCATGGCAAATCCAATCCCCATTGAATAAGc                           >  1:982151/1‑140 (MQ=255)                                                                                                           cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGCCAATCCCCCTTGAATAAGc                           >  1:22175/1‑140 (MQ=255)                                                                                                           cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAGGCCAATCCCCCTTGAATAAGc                           >  1:702597/1‑140 (MQ=255)                                                                                                              tGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCCCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                        <  1:279198/140‑1 (MQ=255)                                                                                                              tGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                        <  2:585542/140‑1 (MQ=255)                                                                                                              tGACCTGGTTTAATTGCAACTATGTATATTGATAAAAATTTTGTACCATATACTAGGTAATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAACCCCCATTGAATAAGCTAc                        <  1:803726/140‑1 (MQ=255)                                                                                                                aCCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg                      <  1:695128/140‑1 (MQ=255)                                                                                                                  cTGGTTTAATTGCATCTTTTTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGa                    <  1:260653/140‑1 (MQ=255)                                                                                                                  cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTACCTTCCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGa                    <  2:916430/140‑1 (MQ=255)                                                                                                                    ggTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACa                  <  2:799928/140‑1 (MQ=255)                                                                                                                       ttAATTGCATCTTTGTACATTGTTAAAAAATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTGCCCTTCTTTTCGAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGcc               <  1:79374/140‑1 (MQ=255)                                                                                                                            tGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa          <  1:483032/140‑1 (MQ=255)                                                                                                                                    tgtaCATTGTTAAAACATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCa  <  1:775106/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCA  >  USA300TCH1516_ALE/1744228‑1744498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑