Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 396,540 G→A A208T (GCA→ACA)  efeM → putative iron uptake system component EfeM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE396,5400GA99.0% 326.7 / NA 104A208T (GCA→ACA) efeMputative iron uptake system component EfeM
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (58/44);  minor base C (1/0);  total (60/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  USA300TCH1516_ALE/396406‑396675
                                                                                                                                      |                                                                                                                                        
tAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACaa                                                                                                                                     <  2:343543/140‑1 (MQ=255)
  gATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACaaaa                                                                                                                                   <  2:540888/140‑1 (MQ=255)
     atCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAt                                                                                                                                <  2:829872/140‑1 (MQ=255)
      tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAtt                                                                                                                               <  2:120340/140‑1 (MQ=255)
       cATACCAAAATTAATGTTACAAGGTTCTGTTGACATATTAAATGAAGTGGCAACTTATAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGAGAACGTTGAAGGCACAAAAAGAAtgt                                                                                                                              >  2:379110/1‑138 (MQ=255)
        aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAGAGATTTATATGATTTTAAAGCGAACGTTGAAGGAACACAACAAAtatg                                                                                                                             >  1:206957/1‑137 (MQ=255)
          aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATg                                                                                                                           <  2:124499/140‑1 (MQ=255)
          aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATg                                                                                                                           >  2:447346/1‑140 (MQ=255)
           ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAACAATTTATGa                                                                                                                          >  1:545385/1‑140 (MQ=255)
           ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATGTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                          >  1:653786/1‑140 (MQ=255)
           ccAAAATTAATGTTACAAGGTTCTGCTGACCTATTAAATGAAGTAGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                          >  2:361311/1‑140 (MQ=255)
            cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTCAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAc                                                                                                                         >  2:552599/1‑140 (MQ=255)
             aaaaTTAATGTTACAAGGTTCTGTTGATCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAAGTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                        <  1:698328/140‑1 (MQ=255)
             aaaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAAGTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                        <  2:277140/140‑1 (MQ=255)
             aaaaTTAATGCTACAAGGTTCTGTTGACCTATTAAATGGAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                        <  1:49972/140‑1 (MQ=255)
              aaaTTTATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACtt                                                                                                                       <  2:122257/140‑1 (MQ=255)
               aaatAATATTACAAGGTGCTGGTGACCTATTAAATTAAGGTGCAACTTCGAAAATCACAGGTGAATAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTa                                                                                                                      <  2:516302/137‑1 (MQ=255)
               aaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTa                                                                                                                      >  1:930274/1‑140 (MQ=255)
                 ttAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAtt                                                                                                                    >  2:89846/1‑140 (MQ=255)
                     tgtgaaaaGGTTCTGTTGACCTATTAAATGGAGTTGCAACTTTTAAAATCACAGGTGAAGAAGAAATTTATTCACATAACGGTTAATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                <  1:436356/134‑1 (MQ=255)
                     tGTTACAAGGTTTTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                <  2:64911/140‑1 (MQ=255)
                     tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                <  1:776935/140‑1 (MQ=255)
                     tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                <  1:317655/140‑1 (MQ=255)
                      gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAc                                                                                                               >  1:321433/1‑140 (MQ=255)
                       ttACAAGGTTCTGTTGACCTATTAAATGACGTTGAAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGTCACACAAAAAATTTATGACTTATTTAAAcc                                                                                                              <  2:297291/140‑1 (MQ=255)
                          cAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACCAAAAATTTATGACTTATTTAAACCTAt                                                                                                           >  1:698131/1‑140 (MQ=255)
                               ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAACATTTATGACTTATTTAAACCTATTTTaa                                                                                                      >  2:532405/1‑139 (MQ=255)
                               ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTag                                                                                                      >  2:531834/1‑140 (MQ=255)
                                  tGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATACACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                   >  1:615924/1‑140 (MQ=255)
                                   gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  1:966958/1‑140 (MQ=255)
                                   gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  2:885563/1‑140 (MQ=255)
                                   gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  2:781837/1‑140 (MQ=255)
                                   gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACAGACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  1:1581/1‑140 (MQ=255)
                                     tGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGATaaaa                                                                                                >  2:450693/1‑140 (MQ=255)
                                     tGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAAAGATTTATATGATTTTAAAGCGAACGTTGAAGACACACAACAAATTTATGACTTATTTAAACCTAATTTGGAGaaaa                                                                                                >  2:441252/1‑140 (MQ=255)
                                      gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGACGCAAGTAATGCAGAGACAGATTTATATGGTGTTATATGTGGCGTAGGTTTTATTTAGATAATTGATGACGTATTTAAACCTATTTTAGAGaaaaa                                                                                               >  2:708453/1‑140 (MQ=255)
                                       aCCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                              >  1:909769/1‑140 (MQ=255)
                                       aCCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATCTACATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATCTATGAATTATTTAAACCTCTTTTACAGaaacaa                                                                                              >  1:179021/1‑140 (MQ=255)
                                             tAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATaaa                                                                                       <  2:909769/140‑1 (MQ=255)
                                                     gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAg                                                                               >  1:584185/1‑140 (MQ=255)
                                                     gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAg                                                                               <  1:405209/140‑1 (MQ=255)
                                                        gCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtga                                                                            >  1:47666/1‑140 (MQ=255)
                                                          aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatg                                                                          <  1:366278/140‑1 (MQ=255)
                                                          aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatg                                                                          <  1:89846/140‑1 (MQ=255)
                                                          aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAAAGATTTATATGATTTTAAAGCGAACGTAGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatg                                                                          <  1:829829/140‑1 (MQ=255)
                                                           aCTTCTAAAATCACAGGTGCCGAAGAAATTTATTCACATACAGATTTATATGATATTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAGCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatga                                                                         >  1:652353/1‑140 (MQ=255)
                                                              tcaaaaaACACACGTGAAGAAGAAACATATTCACATACAGATTTATATGATGGTAAAGCGATCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGatat                                                                      <  1:704403/137‑1 (MQ=255)
                                                               cTAAAATCACGTCGGAATAAGAACTTTATCCACATACAGATTTATAAAATTTTAAAGCCACCGTGAAAGGCAACCCAAAACTTAATAACTTACTTAATTCTATATAAGAAAAAAAAGATAAAAAATTAAGTGATGATATc                                                                     <  2:806438/140‑1 (MQ=255)
                                                               cTAAAATCACAGGTGAAGAAGAAATTTATTGACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATCAAGTGATGATATc                                                                     >  2:383591/1‑140 (MQ=255)
                                                                 aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCa                                                                   >  2:595276/1‑140 (MQ=255)
                                                                   aaTGACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaaa                                                                 <  2:327774/140‑1 (MQ=255)
                                                                     tCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATg                                                               <  2:152457/140‑1 (MQ=255)
                                                                         aGGTGAAGAAGAAATTTATTCACATACAGATTTATCTGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACt                                                           <  1:557047/140‑1 (MQ=255)
                                                                         aGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACt                                                           <  1:150244/140‑1 (MQ=255)
                                                                          ggTGAAGAAGAAGTTTATTCATATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGGAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACtt                                                          >  1:520939/1‑140 (MQ=255)
                                                                          gatGAAGAAGAAATTTATTCACATACAGATTTAAATGCTTTTAAAGCGAAAGTTGAAGTAGCACACAAAATTAAAGAATTATTAAAACCTATTTAAGAGGAAAAAGATAAAGAATCAAATGATGATATCCAAATGAACtc                                                          >  2:158434/3‑139 (MQ=255)
                                                                           gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTc                                                         <  1:152687/140‑1 (MQ=255)
                                                                           gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTCAAAGCGAACGTAAAAAAAAAAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTc                                                         <  2:809807/140‑1 (MQ=255)
                                                                           gTGAAGAAGAAATTTATACACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTc                                                         <  2:280977/140‑1 (MQ=255)
                                                                              aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGAt                                                      <  1:863273/140‑1 (MQ=255)
                                                                              aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAACATAAAAAATTAAGGGATGATATCCAAATGAACTTCGAt                                                      <  1:644111/140‑1 (MQ=255)
                                                                                         tATTCACATACAGAGTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCa                                           >  1:524201/1‑140 (MQ=255)
                                                                                            tCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATCAAGTGAATCAatt                                        >  1:540318/1‑140 (MQ=255)
                                                                                            tCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGCATCAatt                                        >  1:540181/1‑140 (MQ=255)
                                                                                            tCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAAtagatt                                        >  1:178735/1‑135 (MQ=255)
                                                                                             cacaTACAGGTTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatta                                       <  1:850750/140‑1 (MQ=255)
                                                                                              acaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACGAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGAGATGATATCCAAATGAACTTCGATAAAATGAATCAattat                                      >  2:547712/1‑140 (MQ=255)
                                                                                              acaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                      <  2:615924/140‑1 (MQ=255)
                                                                                              acaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                      <  1:447346/140‑1 (MQ=255)
                                                                                                 tACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTgg                                   <  1:633355/140‑1 (MQ=255)
                                                                                                 tACAGATGTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTgg                                   <  1:821497/140‑1 (MQ=255)
                                                                                                      atatataTGATATTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAAAAAAAAAAAAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaaa                              <  2:563831/137‑1 (MQ=255)
                                                                                                           tatGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaa                         >  2:231497/1‑140 (MQ=255)
                                                                                                               aTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGAt                     <  2:888962/140‑1 (MQ=255)
                                                                                                                     aaGCGAACGTTGAAGGCCAACAACAAAATTATGACTTATTTAAACCTAATTTAAAGAAAGAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGAAAAAGTGAATCAATTATTGGAGAAATGTAAAGAGaacaac               >  1:211142/1‑140 (MQ=255)
                                                                                                                     aaGCGAACGTTGAAGGCACACAAAAAATTTATGCCTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac               <  2:321433/140‑1 (MQ=255)
                                                                                                                     aaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac               <  1:856012/140‑1 (MQ=255)
                                                                                                                      aGCGAACGTTGAAGGCACACAAAAAATTTATGAATTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg              >  2:918207/1‑140 (MQ=255)
                                                                                                                      aGCGAACGTCACAGGCACACAAAAAATTTATGACTTATTTAAACCTATCTTAGGGAAAAAAGGTAAAAACTTAAGTGATGATATCCAACTGAACTTACATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg              >  2:231694/1‑140 (MQ=255)
                                                                                                                       gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGGAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGAATAAATATAAAGATAACAAcgg             >  1:669003/1‑140 (MQ=255)
                                                                                                                       gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAAATAAAGATAACAAggg             >  2:90381/1‑140 (MQ=255)
                                                                                                                       gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGACAAAGTGAATCAATTACTGGACAAATATAAAGATAACAAcga             >  2:409125/1‑139 (MQ=255)
                                                                                                                       gCGAACGTTAAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGGATACATATAAAGATaacaaca              >  2:646822/1‑139 (MQ=255)
                                                                                                                        ccaaagTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAATTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc            <  2:245254/135‑1 (MQ=255)
                                                                                                                         gAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcg           >  1:242869/1‑140 (MQ=255)
                                                                                                                         gAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcg           >  1:131783/1‑140 (MQ=255)
                                                                                                                         gAACGTTAAACGCAAACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcg           <  1:853014/140‑1 (MQ=255)
                                                                                                                          aaCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcgg          >  1:488018/1‑140 (MQ=255)
                                                                                                                             gTTGAAGGCACACAAAAAATTTATGACTTATTTCATCCTATTTTTGAGAACGCTGTTAAACCATTACGTCATGTCATTCAAACGAAGTTGGGGAAAGTACACCAACGATCGTAAAAAGAAAAAGATAACAACGCCGGTTa       >  2:335259/1‑140 (MQ=255)
                                                                                                                             gTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa       >  1:419789/1‑140 (MQ=255)
                                                                                                                             gTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa       >  2:47995/1‑140 (MQ=255)
                                                                                                                              ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAATAACGGCGGTTAt      >  1:351042/1‑140 (MQ=255)
                                                                                                                              ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt      >  1:923717/1‑140 (MQ=255)
                                                                                                                              ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTGAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAATTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt      >  1:86499/1‑140 (MQ=255)
                                                                                                                               tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAGAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg     >  1:664006/1‑140 (MQ=255)
                                                                                                                                gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGGATAAATATAAAGATAACAAAGGCGGTTATGa    >  2:46113/1‑140 (MQ=255)
                                                                                                                                gAAGGCACACAAAAAATTTATGACTTATTTAAACATATTATAGATAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGACAACAACGGCGGTAATGa    >  2:412319/1‑140 (MQ=255)
                                                                                                                                  aGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAGAGATAAAAAATTAAATGATAATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCTGTTATGAGt  >  2:635205/1‑140 (MQ=255)
                                                                                                                                  aGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGt  <  1:595276/140‑1 (MQ=255)
                                                                                                                                  aGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATAAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGCCGGTTATGAgc  >  2:635502/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  USA300TCH1516_ALE/396406‑396675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: