| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0% | 193.3 / NA | 59 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (14/45); total (14/45) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA > USA300TCH1516_ALE/2532161‑2532433 | tttgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATGATTAATAAATTGAAAAATTAGAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTactaca < 1:535671/139‑1 (MQ=255) ttATGTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 2:53818/140‑1 (MQ=255) gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 1:244078/141‑1 (MQ=255) gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 1:325373/141‑1 (MQ=255) tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc < 2:62163/141‑1 (MQ=255) tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt > 2:194730/1‑141 (MQ=255) aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc > 2:57119/1‑141 (MQ=255) aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc < 1:407849/141‑1 (MQ=255) tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa < 1:501483/141‑1 (MQ=255) tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa < 1:35135/141‑1 (MQ=255) taaaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGTGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAAGGCGTTTACTACAAAGCATTTTTCACATTacacac < 2:554354/138‑1 (MQ=255) gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTTACGTTGACTTCGGATGAGTTCACTGCGTTTACTACAAAGCATTTTGCACATTacccac > 1:285871/1‑141 (MQ=255) gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAG‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac < 2:462730/141‑1 (MQ=255) ataaaagaaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca < 2:346875/141‑1 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt > 2:515791/1‑141 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt < 1:325385/141‑1 (MQ=255) aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTCCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt < 2:195929/141‑1 (MQ=255) aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa > 1:247830/1‑141 (MQ=255) aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa > 2:545254/1‑141 (MQ=255) aaGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAtt < 1:81078/140‑1 (MQ=255) gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc < 2:25197/141‑1 (MQ=255) gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc < 2:51002/141‑1 (MQ=255) gCATTATTAATAAATTAAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc < 1:549427/141‑1 (MQ=255) cATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa > 1:579845/1‑141 (MQ=255) taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa < 2:95763/141‑1 (MQ=255) ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag < 2:450211/141‑1 (MQ=255) aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGt < 1:313567/141‑1 (MQ=255) aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTATAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCAAGTTACACACAATCACCTATACATTACAGTCATAGAATTg > 2:71907/1‑141 (MQ=255) aaaaTTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTCCACACAATCAGCTATTCATTACAATAATAGGCTTGCTTTa > 2:666700/1‑141 (MQ=255) aaaaTTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa < 1:685774/141‑1 (MQ=255) aaaaTTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa < 1:455006/141‑1 (MQ=255) aTTATAGTTCAAGTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaa < 2:513150/141‑1 (MQ=255) ttCAATTCAGAGATGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGTATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg < 2:523665/141‑1 (MQ=255) ttCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg < 2:608936/141‑1 (MQ=255) gtCAATCCAGAGAGGGAAGGGAT‑AAAATGAACTTTGTAATGTTGACTTCGGCTGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg < 2:313703/140‑1 (MQ=255) aTTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGGGTTGATTTAAAAGGCGATGTGcc > 1:229397/1‑140 (MQ=255) tCAGAGAGGGATGGGAT‑AAAATGAACTTTGGAACGTTGACTTCGGAAGAGTTCTATTCGTTTACTAAAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc < 1:333270/141‑1 (MQ=255) agagGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGt < 2:519604/141‑1 (MQ=255) tggat‑CAAATGCAATTTGTGCCGTTGACTTCGGATGAGTTCAATGCGCTTGCTACGAAGCGTTTGTCACGTTACACGCAATCAGCTATTCATTGCAATCATAGTGGTGATTTAAAAGGCGATGTGCATCATGTAGGGGTTa < 2:750479/140‑1 (MQ=255) gggat‑AAAATTAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa < 2:435159/141‑1 (MQ=255) ggat‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa < 2:77279/141‑1 (MQ=255) ggat‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa > 1:473749/1‑141 (MQ=255) at‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 2:585835/141‑1 (MQ=255) aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg < 2:740830/141‑1 (MQ=255) aaaTGAACTTTGTAACGTGGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg < 2:227442/141‑1 (MQ=255) acgAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAc < 2:763285/139‑1 (MQ=255) tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCATAAAAAGCATTTTTCACATTACATCCCATCATCTATTCATTAAAATTATGGAGGTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa < 1:753981/141‑1 (MQ=255) tttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGt < 1:682836/141‑1 (MQ=255) ggtGACTTCGGATGGGTGTGATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAGCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt < 2:290233/139‑1 (MQ=255) gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGTGAATGGTCAAGTGAt < 2:87269/141‑1 (MQ=255) gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt < 1:194730/141‑1 (MQ=255) tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg < 1:515791/141‑1 (MQ=255) tGACTGCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAAAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg < 2:283/141‑1 (MQ=255) gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc > 2:143250/1‑141 (MQ=255) aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAGAGTCAAGTGATTGcg > 2:414450/1‑140 (MQ=255) ttCGGAGGAGTTCATTTCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTATAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg < 2:597556/141‑1 (MQ=255) gaaTGAGTTCAATGCGTTTACTACAAAGCATTCTGCACATTACACACAATCAGCTATTCATTACAATCATAGGGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg < 2:285871/139‑1 (MQ=255) tGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAACAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt > 2:490609/1‑141 (MQ=255) attcGTTTACTACAAAGCATTCTTCACATTACACACAATCAGCTACTTCTTACAATTCTAGAGTTGATTTAAAAGGCGACGTGCATCTTGTTGGGGGTAAAGATGACAATGGTCAAGCGATTGCCGGATGCTTATTGACAg < 2:279680/138‑1 (MQ=255) tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca < 1:349790/141‑1 (MQ=255) | TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGAT‑AAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA > USA300TCH1516_ALE/2532161‑2532433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |