Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,698,922 T→C 6.5% R745R (CGT→CGC)  copA → Copper‑exporting P‑type ATPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,698,9220TC 6.5% 178.4 / 2.2 69R745R (CGT→CGC) copACopper‑exporting P‑type ATPase A
Reads supporting (aligned to +/- strand):  ref base T (34/30);  new base C (3/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

TGATATCGGTATCGCCATTGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTCACAAACGCACTTAG  >  USA300TCH1516_ALE/2698790‑2699053
                                                                                                                                    |                                                                                                                                   
tGATATCGGTATCGCCATTGGTACAGGTACAGAAGTAGCCAGGTAGGCAGCTGATATTACTATTCTTGGTGGCGACTTGCTGCTTATTCCCAAAGCCATTTATGCAGGTAAAGCAACCATTCGTAATATTCGCCACActct                                                                                                                             >  2:576207/1‑141 (MQ=255)
tGATATCGGTATCGCCATTGATACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATACTTATTGCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCt                                                                                                                             >  1:509288/1‑141 (MQ=255)
    atCGGTATCGCCATTGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTAttt                                                                                                                         >  2:133758/1‑141 (MQ=255)
      cGGTATCGCCATTGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACAATGCGTAATATTCGTCAAAATCTATTTTg                                                                                                                       >  1:15484/1‑141 (MQ=255)
       agTAACGCCATTGGTACAGGGAAAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTAGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTgg                                                                                                                      <  2:441434/140‑1 (MQ=255)
        ttATCGCGATGGGTACAAGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTCATTCCTAAAGACATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTggg                                                                                                                     <  2:5607/140‑1 (MQ=255)
        tgagcgacATTGGTACAGGTACAGAAGTCGCCATTGAAGCAGCGTATTTTAGTATTCTTGGGGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTggg                                                                                                                     <  2:376579/134‑1 (MQ=255)
              ccATTGGTACAGGTACCGACGGTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCg                                                                                                               >  1:759174/1‑141 (MQ=255)
              ccATTGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCg                                                                                                               >  1:542516/1‑141 (MQ=255)
                gtgggtacaggtacaGATGTTGCCATTGCAGCAGCTTGTATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGc                                                                                                             <  2:363836/138‑1 (MQ=255)
                 ttGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCt                                                                                                            >  1:688987/1‑141 (MQ=255)
                      acaggtacagAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTataat                                                                                                       >  2:367565/1‑141 (MQ=255)
                       caggtacagAAGTTGCCATTGAAGCAGGTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTataata                                                                                                      >  2:70231/1‑141 (MQ=255)
                          gtacagAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTg                                                                                                   <  2:290367/141‑1 (MQ=255)
                          gtacagAAGTGCCCATTTAAGCAGCTGATATTATTATTCTTGGTGGCGTCTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTAGAATATTg                                                                                                   <  2:368956/141‑1 (MQ=255)
                             cagAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCg                                                                                                >  2:602513/1‑141 (MQ=255)
                              agAAGTTGCCATTGAATCAGCTGAGATTACAATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCGTTCGTAATATTCGTCAAAATCTATTTTGGGCATGCGGCTATAATATTGCCgg                                                                                               <  2:609987/141‑1 (MQ=255)
                                  gTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATc                                                                                           <  1:376912/141‑1 (MQ=255)
                                        aTTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCtata                                                                                     <  2:714868/141‑1 (MQ=255)
                                         ttGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAg                                                                                    <  1:133758/141‑1 (MQ=255)
                                         ttGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAg                                                                                    >  1:303543/1‑141 (MQ=255)
                                            aaGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTg                                                                                 >  2:217246/1‑141 (MQ=255)
                                            aaGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATCCGGCTATAATATTGCCGGTATCCCTATAGCTg                                                                                 <  2:86157/141‑1 (MQ=255)
                                              gcagcTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCa                                                                               >  2:243219/1‑141 (MQ=255)
                                              gcagcTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCa                                                                               >  2:617701/1‑141 (MQ=255)
                                                  cTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGCCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTgg                                                                           >  1:312919/1‑141 (MQ=255)
                                                  cTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGCCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTgg                                                                           >  1:407979/1‑141 (MQ=255)
                                                   agATATTACTATTCTTGGTGGCTACTTGATGCATAATCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTAATTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTggg                                                                          <  2:301976/140‑1 (MQ=255)
                                                    gATATTACTATTCTTGGGGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTgggg                                                                         >  1:415294/1‑140 (MQ=255)
                                                        ttACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTCTAGCTCCATTGGGCTTAc                                                                     >  1:82324/1‑141 (MQ=255)
                                                         tACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACt                                                                    <  1:578015/141‑1 (MQ=255)
                                                          aCTATTCTTGGTGGAGACTAGATGCTTATTCCTAAAGCCATTTAGGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACtt                                                                   >  1:420169/1‑141 (MQ=255)
                                                           cTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTg                                                                  >  2:546656/1‑141 (MQ=255)
                                                            tATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTCCTTgc                                                                 >  1:421876/1‑141 (MQ=255)
                                                             aTTCTTGGTGGCGCCTTGAGGCATATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATGTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTgcg                                                                <  2:176210/141‑1 (MQ=255)
                                                             aTTCTTGGTGGCGACTTGATGCTTATTCCAAAGGCCATTTATGCAAGTAAAGAAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTgcg                                                                <  1:329739/141‑1 (MQ=255)
                                                              ttCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTgcgc                                                               >  2:97070/1‑141 (MQ=255)
                                                                tttGGTGGCGACTTTATGCTTATGCTGAAAGCTATTTATGCAAGGAAAGCAATCATTTGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCa                                                             <  1:537467/140‑1 (MQ=255)
                                                                 ttGGTTGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTATAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCAt                                                            <  2:275037/141‑1 (MQ=255)
                                                                 ttGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTCTCCCTATAGCTGCATTGGGCTTACTTGCGCCAt                                                            >  1:329200/1‑141 (MQ=255)
                                                                         gACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCt                                                    >  1:367317/1‑141 (MQ=255)
                                                                          aCTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCACTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTg                                                   >  1:564667/1‑141 (MQ=255)
                                                                           tttGAAGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTAACCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTgg                                                  <  2:145709/140‑1 (MQ=255)
                                                                           ctggATGCTTATGCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTgg                                                  <  2:172474/138‑1 (MQ=255)
                                                                           cTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTgg                                                  >  2:528697/1‑141 (MQ=255)
                                                                           attaaTGCTTATTCCTAAAGCCATTTAAGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTgg                                                  <  2:575125/137‑1 (MQ=255)
                                                                             tGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGtg                                                <  1:374957/141‑1 (MQ=255)
                                                                               attattATCCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCTGCTATAATATTGCCTGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGtgct                                              <  2:695601/137‑1 (MQ=255)
                                                                                  cTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATAGGGCTTCCTTGCGCCATGGGTTGCTGGTGCTGCa                                           >  2:246124/1‑141 (MQ=255)
                                                                                       tCCTACAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGCCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGc                                      <  1:33758/141‑1 (MQ=255)
                                                                                         cTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTGTTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCAc                                    <  1:367565/141‑1 (MQ=255)
                                                                                         cTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCAc                                    >  2:294835/1‑141 (MQ=255)
                                                                                          tAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACt                                   >  2:307476/1‑141 (MQ=255)
                                                                                          tAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACt                                   >  1:424695/1‑141 (MQ=255)
                                                                                           aaaGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTa                                  <  2:453878/141‑1 (MQ=255)
                                                                                            aaGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTaa                                 <  2:303543/141‑1 (MQ=255)
                                                                                              atcaattAGGCAAGAAAAGGAAACATTCGTAATATTCGTTAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCCGGTGCTGCAATGGCACTAAGt                               <  2:188636/136‑1 (MQ=255)
                                                                                                  tttATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGTCATGGATTGCTGATGCTGCAATGGCACTAAGTTCAg                           >  1:134310/1‑141 (MQ=255)
                                                                                                  tttATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCCATGGCACTAAGTTCAg                           >  2:475909/1‑141 (MQ=255)
                                                                                                   ttATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGt                          <  1:72146/141‑1 (MQ=255)
                                                                                                        cAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAgtg                     >  1:63758/1‑141 (MQ=255)
                                                                                                             aaaGCAACCATTCGTAATATTCGCCAATATCTATTTTGGGGCATCTGCTATAAGATTGCCGGTATCCCTATAGCTGCATTGGGCTTACCTGCGCCATGCGTTGCATGTGCTTCATTTGCACTACGTTCAGTAAGttttggc                <  2:312919/141‑3 (MQ=255)
                                                                                                             aaaGCAACCATTCGTAATATTCGCCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTc                <  2:407979/141‑1 (MQ=255)
                                                                                                              aaGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTca               <  1:420631/141‑1 (MQ=255)
                                                                                                                 cAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTCACaa            >  1:738036/1‑141 (MQ=255)
                                                                                                                 cAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTCACaa            <  2:759174/141‑1 (MQ=255)
                                                                                                                 cAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTATTGTCACaa            >  1:69872/1‑141 (MQ=255)
                                                                                                                     cATCCGTAATATTCGTCAAAAACTCTTTTGAGCATTCGGCTATAATATTGCCGGTATCCCAAAATCTGCATTGGGCTTCCTTTCGCCATGGGGTACTGGTGCTCTAATGGCACTTGGTTCAGTAAGTGTTGTCACAAACGc        >  2:179482/1‑141 (MQ=255)
                                                                                                                           tAATATTCGTCAAAATATATTTTGGGCATTAGGCTATAATATTACAGGCACGGAGATGGCGGTGTTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGGTCAGTAAGTGTTGTCACAAACGCACTGAg  >  2:735110/1‑141 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TGATATCGGTATCGCCATTGGTACAGGTACAGAAGTTGCCATTGAAGCAGCTGATATTACTATTCTTGGTGGCGACTTGATGCTTATTCCTAAAGCCATTTATGCAAGTAAAGCAACCATTCGTAATATTCGTCAAAATCTATTTTGGGCATTCGGCTATAATATTGCCGGTATCCCTATAGCTGCATTGGGCTTACTTGCGCCATGGGTTGCTGGTGCTGCAATGGCACTAAGTTCAGTAAGTGTTGTCACAAACGCACTTAG  >  USA300TCH1516_ALE/2698790‑2699053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑