BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,981	A→G	9.7%	D735D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,981	0	A	G	9.7%	184.0 / 2.1	85	D735D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (39/34); new base G (3/6); total (43/42)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGT  >  USA300TCH1516_ALE/2774841‑2775120

gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatccgaa                                                                                                                                             >  2:77776/1‑141 (MQ=255)

  atcACTGTCTGAATCCGAATCCCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                           <  1:457820/141‑1 (MQ=255)

    cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTGCGAGTCGCTATCTGAATCTGAGTCGCTGTTTGAGTCGGAGGCGATatctgtatgtg                                                                                                                                         >  2:228066/1‑141 (MQ=17)

      cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                       <  2:730152/141‑1 (MQ=255)

      cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                       >  1:730661/1‑141 (MQ=255)

         tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTCTCTGAGTCTGAGTCg                                                                                                                                    >  2:586982/1‑141 (MQ=255)

         tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                                    >  1:180150/1‑141 (MQ=255)

         tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                                    >  1:675016/1‑141 (MQ=255)

          cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                                                                                                   >  1:682217/1‑141 (MQ=255)

                  gAATCGCAATCGGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGACCCTAcctgag                                                                                                                           >  2:641094/1‑141 (MQ=14)

                    aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGGTAgctgagtc                                                                                                                         >  2:65732/1‑141 (MQ=255)

                     tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGCTTCGCTATCTGCATCTGAGTCGCTAtctcagtct                                                                                                                        >  1:646575/1‑141 (MQ=255)

                        cTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGGGTCGCTGtctgagtttgag                                                                                                                     >  2:635144/1‑141 (MQ=14)

                          ctctgaatctgaaTCACTATCCGAGTCCGAGTCGCTCTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                                   <  2:691356/140‑1 (MQ=255)

                           tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgagtctgagtcg                                                                                                                  >  2:258082/1‑141 (MQ=255)

                           tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctaagcctgagtcg                                                                                                                  >  2:391914/1‑141 (MQ=255)

                            ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                                 >  1:179264/1‑141 (MQ=255)

                              gaatctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCGGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgcta                                                                                                               >  1:538718/1‑140 (MQ=255)

                                    gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGgctgagtctgagtcgctgtctgag                                                                                                         >  2:640529/1‑141 (MQ=255)

                                    gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgag                                                                                                         <  1:599878/141‑1 (MQ=255)

                                      gtcGCTTTCCGAGTCTGAGTCGTTGTCTGAGTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                       <  2:154376/140‑1 (MQ=14)

                                      atcGTTAGCTGAGTCTGAGTCGTTGTCTGAATCTGATGCGCTGTCTGTGTCCGAATCGCTCTCTGATTCTGTGTCGCTGTTTGAGACTGAATCGCTATCAGAGTCTGAGTTGCTAtctgactctgagtcgctgtctgagtc                                                                                                       <  2:724029/141‑1 (MQ=14)

                                      atcGCTATCCGAGTCTGGGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                       <  1:594947/141‑1 (MQ=255)

                                      atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                                       <  2:441786/141‑1 (MQ=255)

                                       tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGCATAGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgaggctgacacgctgtctgagtct                                                                                                      >  2:500677/1‑141 (MQ=14)

                                        cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctg                                                                                                     <  1:494356/141‑1 (MQ=255)

                                         gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGGATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgg                                                                                                    >  2:684924/1‑140 (MQ=255)

                                         gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctga                                                                                                    <  1:394497/141‑1 (MQ=255)

                                         ccTATCCGACTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctga                                                                                                    <  2:581459/140‑1 (MQ=255)

                                          tcaTCCCAGTTTTAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCCGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                   <  2:628909/139‑1 (MQ=255)

                                          cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctggg                                                                                                   >  1:644330/1‑139 (MQ=255)

                                          cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                                                                                   >  2:486407/1‑141 (MQ=255)

                                            ttCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                                 <  1:714820/140‑1 (MQ=255)

                                            ctCCGAGTCTGAGCCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                                 <  1:297866/140‑1 (MQ=255)

                                              ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                                                                               >  2:315520/1‑141 (MQ=255)

                                                gAGTCTGCGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgctg                                                                                             >  1:483449/1‑141 (MQ=255)

                                                 ggTCTGAGTCGCTGTCTGAATCCGAATCGCTTTCTGAGTCCGAATCGCTATCTGAATCTGAGTCCCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATctgagtctgagtcgctgtctgagtctgagtcgctgt                                                                                            <  2:12308/140‑1 (MQ=255)

                                                    ttGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcgctgtctgagtctgagtcgctgtctg                                                                                         <  1:547535/140‑1 (MQ=255)

                                                      gAGTCGCTGTCTGATTCTGATTCGCTTTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                                       <  2:340479/141‑1 (MQ=14)

                                                        gTCGCTGTCTGAATCTGAATCGCTCTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                                     >  1:560072/1‑141 (MQ=255)

                                                          cGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCTGCATCTGAGTCGCTGTCTGAGTCtgattcactgtatgcatctgagtccctgtctgagtcgc                                                                                   >  2:226108/1‑139 (MQ=11)

                                                          cGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAAGCTGGGTCGCTGTCTGAGTCTGGGTCGCTGTCTGACTCTTATACGCGGTCTGagtctcgttcgatgtctgagtctgagacgctgtctgggagtc                                                                                   >  2:226037/1‑135 (MQ=2)

                                                          cGCTGAATGATTTTGCATCGCTGTCAGAGTCCGATGCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                                                                                   <  1:746622/141‑1 (MQ=14)

                                                           ccTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtctgagtctga                                                                                  <  2:707488/140‑1 (MQ=255)

                                                            cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgcggtctgagtctgagtcgctgtctgaggctgga                                                                                 >  2:245046/1‑139 (MQ=255)

                                                               tctgagtctgaaTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                              <  1:601404/141‑1 (MQ=255)

                                                               tctgaatctgaaTCGCTGTCTGAGTCCGAATCGCTACCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                              <  1:415151/141‑1 (MQ=255)

                                                                ctgaatctgaatCGCTATCTGAGTCCGAATCGGTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                                                                             <  2:439740/141‑1 (MQ=255)

                                                                 tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                            >  1:323721/1‑77 (MQ=35)

                                                                  gaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                           <  1:636099/141‑1 (MQ=255)

                                                                   aatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTat                                                                          <  1:229064/141‑1 (MQ=255)

                                                                    atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                         >  1:212070/1‑141 (MQ=255)

                                                                    atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAAACGCTatc                                                                         >  2:412456/1‑141 (MQ=255)

                                                                    atctgaatcCCTGCCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                         <  2:412699/141‑1 (MQ=255)

                                                                       tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                      <  1:616504/141‑1 (MQ=255)

                                                                        gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTCTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTCTCTGGGTCTTAATCGCTatatgaa                                                                     >  2:309876/1‑141 (MQ=14)

                                                                        gaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCGGGCGGAGTCTGAGGCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                     >  1:399635/1‑141 (MQ=255)

                                                                         actcGTTGTCCGAGTCCGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                    <  2:378570/139‑1 (MQ=14)

                                                                          atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                   >  1:82274/1‑141 (MQ=255)

                                                                          atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                   <  1:374896/141‑1 (MQ=255)

                                                                             tctatCTGCGTCCGAGTCGCTATCTGAATCTGAGTCGCCGTTCGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                <  2:300158/137‑1 (MQ=14)

                                                                              cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGGATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTATGAGTCTGAGTCGCTGTATGAGTCTGAATGGCTATATGAATCTGAg                                                               >  1:12481/1‑141 (MQ=17)

                                                                                   tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtctggatctgaatggcg                                                          >  2:627773/1‑135 (MQ=255)

                                                                                   tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGGGTCGCt                                                          >  1:310226/1‑141 (MQ=255)

                                                                                     aGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGACTCACTgt                                                        >  2:217715/1‑141 (MQ=255)

                                                                                       tCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGGCGCTgcct                                                      >  2:485527/1‑141 (MQ=255)

                                                                                        ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                     >  1:571973/1‑141 (MQ=255)

                                                                                          gAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                                   <  1:27504/141‑1 (MQ=255)

                                                                                          gAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                                   <  1:596750/141‑1 (MQ=255)

                                                                                            aTCACTATCTGAATCTGAGTCGCTGTCTGAGGCTGAATCGCTATCTGAATCTAAGTCCCTATCTGAACCTGAGTCCCGGTCTGAGTCTGTCTCACTGTCTGAGTCTGGATCGCTATCTGAATCTGAGGCGCTgtctgagtc                                                 >  2:328840/1‑141 (MQ=14)

                                                                                              cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                               >  1:623179/1‑141 (MQ=255)

                                                                                                  ttctgactcttaTTCGCGGTCTGTGCTTGAGTCGCTTTCTGCATCTGAGTCGCTATCTGGGTCTGAGTCGCTGTCTGAGTCCGAGTCGCTGTCTGAGTTTGAATCGCTATCTGACTCTGAGTCGCCGTCtgagtccgagtc                                           <  2:106155/140‑1 (MQ=14)

                                                                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTTTGAATCGCTTTCTGAATATGAATCGCTgtctgagtctgaatgg                                          >  2:275179/1‑137 (MQ=14)

                                                                                                   tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                          <  1:228066/141‑1 (MQ=255)

                                                                                                   tctgaatctgaGTCGCTGTATGATTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTTAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTATGAATCTGAATCGCTGTCGGCAtcagagtcg                                          >  2:354523/1‑141 (MQ=11)

                                                                                                        atctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                     >  1:252889/1‑141 (MQ=255)

                                                                                                        acctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcactgtc                                     >  2:702173/3‑141 (MQ=255)

                                                                                                           cctggcGCTGTCTGAGTCTGAGTCGCTATCTGATTCTGAGTCGCTATCTGTGTCTGGGTCGCTGTCTGAGTCTGAGTCGCTGTTTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                                  <  2:377390/136‑1 (MQ=17)

                                                                                                             cgttgCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                <  2:236744/137‑1 (MQ=255)

                                                                                                             aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaat                                <  1:486407/141‑1 (MQ=255)

                                                                                                              gTCGCTGTCGGTGTCTGTGTCGCTTTCTGAGGCTGTGTCGCTGTCTGAGTCTGTGTCGCTGTCTGGGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatc                               <  2:406030/141‑1 (MQ=14)

                                                                                                                cGCTGTCTGCGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                             <  2:272582/141‑1 (MQ=255)

                                                                                                                   tGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagt                          >  2:385083/1‑141 (MQ=255)

                                                                                                                        gaatctgaGTCGCTATCTTAGTCTGTGTCGCTATCCGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                     <  2:355197/138‑1 (MQ=11)

                                                                                                                              gaAGCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctgaa               <  1:649307/141‑1 (MQ=255)

                                                                                                                               agtCGCCATCTGAGTCTGAGTCGCTGTCTGAGTCTGATTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTctgagtcgctgtctgaatctgagtcgctgtctgaat              <  2:286860/139‑1 (MQ=14)

                                                                                                                                ggcGCTTTCTGCATCTGAGTCGCTTTCCGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGTCTGAGTCCGGATCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctgtttg             <  2:319698/139‑5 (MQ=14)

                                                                                                                                  ctgtctctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTCTCTTAGTCTGAGTCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg           <  2:296636/136‑1 (MQ=14)

                                                                                                                                    cTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGtctgaatctgaa         >  1:468559/1‑141 (MQ=255)

                                                                                                                                     tATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGCCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTctgaatctgaat        <  1:210233/141‑1 (MQ=255)

                                                                                                                                      ttctgagtccgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCtgaatctgaatc       <  2:35616/140‑1 (MQ=255)

                                                                                                                                       tctggatctggGTCGCTATCTGAGTCTGAGTCGCTGTCTTAGTCTTTGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCGGAGTCGCTGTCTGAGTCCGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCg      <  2:212070/141‑1 (MQ=17)

                                                                                                                                       tctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCg      >  1:657245/1‑141 (MQ=255)

                                                                                                                                       tctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGCCGCTGTCTAAACCTGAcggacttactgaatctgagtcgctgtctgagtccgattgg      >  2:159503/1‑137 (MQ=14)

                                                                                                                                       tctgaatctgaGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTGGCTGTCTGTATCTGAGTCACTGTCTGAATCTGAATCg      >  2:201645/1‑141 (MQ=255)

                                                                                                                                       tctgaagctgaGTCGCTCTCTTAGTCTGAGTCGCTGTCTGAGTCTGAGGCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCg      <  1:332686/141‑1 (MQ=255)

                                                                                                                                        ctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGc     <  1:323984/141‑1 (MQ=255)

                                                                                                                                           aatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCGGAATCTGAATCGCTgt  >  2:757633/1‑141 (MQ=255)

GAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGT  >  USA300TCH1516_ALE/2774841‑2775120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`