Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 72,351 A→G 5.9% L208S (TTA→TCA)  arcD_1 ← Arginine/ornithine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE72,3510AG 5.9% 233.6 / 2.2 85L208S (TTA→TCA) arcD_1Arginine/ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base A (35/44);  new base G (3/3);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

TAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGAAATATAGGT  >  USA300TCH1516_ALE/72212‑72491
                                                                                                                                           |                                                                                                                                            
tAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTCAATAACCATACAGTTACTaa                                                                                                                                             >  2:308647/1‑141 (MQ=255)
  gTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTTAATAACCATACAGTTACTAAcc                                                                                                                                           >  2:256148/1‑140 (MQ=255)
      aGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGt                                                                                                                                       >  2:492272/1‑141 (MQ=255)
      aGTCATAAAGAAATAAATAACTAGCACAGAAATCAAACCAATAACGGTCGCAGTTCCAACATCCTTTTTAGACTTAGCACGACACGAAAAGACAACGGTTCCTTCAATCCCTGTAGCTACTCAAACAATTACTAACATacg                                                                                                                                       >  2:30604/1‑139 (MQ=255)
        tCATTAAGAAATAAATGACTAGCACAGAAAGCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACGACGGGGCCTTAAATCCCGATGAATAGCCATACAGTTACTGACATAGTAc                                                                                                                                     >  1:672696/1‑141 (MQ=255)
         cATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACt                                                                                                                                    <  1:613449/141‑1 (MQ=255)
          actcaGAAATAAATGACTAGCACAGAAATCAAACCAATTACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACAGAGTAttt                                                                                                                                   <  2:90209/137‑1 (MQ=255)
           ttAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACttt                                                                                                                                  >  2:680013/1‑141 (MQ=255)
             aaGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTACAATCCCTGTGAATACCCATACAGTTACTAACATAGTACttttt                                                                                                                                <  2:410705/141‑1 (MQ=255)
              aGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCACACAGTTACTAACATAGTACTTTTTa                                                                                                                               >  2:441892/1‑141 (MQ=255)
               gAAATAAATGACTAGCACATAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTAc                                                                                                                              <  2:41503/141‑1 (MQ=255)
                 aataaatGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACtt                                                                                                                            <  1:339259/141‑1 (MQ=255)
                       tGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTGACATAGTACTTTTTACTTGTGCCa                                                                                                                      >  1:623667/1‑141 (MQ=255)
                       tGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTGACATAGTACTTTTTACTTGTGCCa                                                                                                                      >  1:456384/1‑141 (MQ=255)
                        gACTAGCACAGAACTCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCAt                                                                                                                     <  2:92727/141‑1 (MQ=255)
                        gACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAATATAGTACTTTTTACGGGTGCAAt                                                                                                                     <  2:547651/141‑1 (MQ=255)
                          cTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTTTTTGTGTCAttt                                                                                                                   >  2:437363/1‑140 (MQ=255)
                              cacaGAAATCAAACCAGCAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTAtc                                                                                                               <  2:15979/141‑1 (MQ=255)
                              cacaGAAATCAAACCAATACCGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTAtc                                                                                                               <  2:396166/141‑1 (MQ=255)
                              cacaGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTAtc                                                                                                               <  1:591920/141‑1 (MQ=255)
                               acaGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTTtct                                                                                                              >  1:49312/1‑141 (MQ=255)
                               acaGAAATCAAACAAATAACGGAAGCAGTTCATACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGCATACCCCTACAGTTACTATCGAAATAGTTTTTACTTTGGCGATTGTaacc                                                                                                              >  2:347284/1‑138 (MQ=255)
                                 aGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATCGTACTTTTTACTTGTGCCTTTTTATCTcc                                                                                                            >  2:675231/1‑141 (MQ=255)
                                 aGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTCTTTAGACTTTGCACGGCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGGATcccc                                                                                                            >  2:740060/1‑141 (MQ=255)
                                  gAAATCAAACCAATAACGGGAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAAACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTAATCGTGCCATTGTATCTccc                                                                                                           >  2:608992/1‑141 (MQ=255)
                                   aaaTCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTcccc                                                                                                          <  1:443142/141‑1 (MQ=255)
                                    aaTCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTGGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCa                                                                                                         >  1:406633/1‑141 (MQ=255)
                                      tCAAGCCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAAc                                                                                                       <  2:95717/141‑1 (MQ=255)
                                        aaaCGAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTa                                                                                                     >  1:523535/1‑141 (MQ=255)
                                         ccccAATAACGGTAGCAGTCCCTACATCCCTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTaa                                                                                                    <  2:10241/139‑1 (MQ=255)
                                         aaCCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTaa                                                                                                    <  1:753283/141‑1 (MQ=255)
                                          aCCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTaaa                                                                                                   >  2:237326/1‑141 (MQ=255)
                                                 aCGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTATTTTTTCTTTTGTATCTCCCCAACTAAAAACGCCa                                                                                            >  2:756351/1‑141 (MQ=255)
                                                 aCGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCa                                                                                            >  2:756307/1‑141 (MQ=255)
                                                 aCGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCa                                                                                            >  2:517085/1‑141 (MQ=255)
                                                 aCGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCa                                                                                            >  1:189799/1‑141 (MQ=255)
                                                    gTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAaca                                                                                         <  1:336209/141‑1 (MQ=255)
                                                      aGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACt                                                                                       >  2:744242/1‑141 (MQ=255)
                                                      aGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACt                                                                                       <  2:765742/141‑1 (MQ=255)
                                                           ttCCTACATCCTTTTTAGCCTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCAc                                                                                  <  2:647997/141‑1 (MQ=255)
                                                           ttCCTACATCCTTTTTAGACTTTGCACGTCCAGCAACGACAACGGCTCCTTCACTCCCTGTACCTACCCATACAGTCACGAACATAGTACTATTTACTTGTGCCATTGTATCGCCCCAACTAAAAACGCCAACACTTCCAc                                                                                  <  2:561384/141‑1 (MQ=255)
                                                           ttCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTAATACCATTGTTCTCTTTACTTGTGCCCTTGTATCTCCCCAACTAAAAACGCCAACACTTCCAc                                                                                  <  2:162259/141‑1 (MQ=255)
                                                           ttCCTACATCCTTTTGAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCAc                                                                                  <  2:552352/141‑1 (MQ=255)
                                                            tCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACt                                                                                 >  2:545950/1‑141 (MQ=255)
                                                            tCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAAACGACATCTCCTTCAAACCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACt                                                                                 >  1:369539/1‑141 (MQ=255)
                                                                 cATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCa                                                                            >  1:744036/1‑141 (MQ=255)
                                                                  aTCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACACCAACACTTCCACTAGTCAt                                                                           <  2:41126/141‑1 (MQ=255)
                                                                        tttAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATa                                                                     >  1:506575/1‑141 (MQ=255)
                                                                           aGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATaaaa                                                                  <  1:10314/141‑1 (MQ=255)
                                                                             aCTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAAc                                                                >  1:305724/1‑141 (MQ=255)
                                                                                  gCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGAt                                                           >  1:367948/1‑141 (MQ=255)
                                                                                  gCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGAt                                                           >  2:517400/1‑141 (MQ=255)
                                                                                     cGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTa                                                        >  2:168042/1‑141 (MQ=255)
                                                                                        ccAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTaaaa                                                     <  1:680013/141‑1 (MQ=255)
                                                                                           taaaaGCCGACTGCTCCTTCATTCCCTGTAAATCGCCATACAGTTACTAACCTCGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCCACCCTCCCACTAGTCATACCATAAAAACCGGATTTAAAAGTa                                                  <  2:504741/140‑1 (MQ=255)
                                                                                           gAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTa                                                  <  2:95017/141‑1 (MQ=255)
                                                                                           gAAAAGACAACGGCTCCTTCAATCCCTGGGAATACCCACACAGTCACTACCATGGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTa                                                  <  1:213082/141‑1 (MQ=255)
                                                                                            aaaaaaCAACGGCACCTTCAATCCCTGAAAATACCCATATAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACCCTTCCACTAAAAAAAAAAAAAAAACCGGATTTAAAAGTAc                                                 <  2:126864/141‑1 (MQ=255)
                                                                                             aaaGTCAACGGCCCCTTCAAACCCTGTGAAGACCCATACAGTTACTAACATAGTACCTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACt                                                <  2:672696/141‑1 (MQ=255)
                                                                                                  cAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGt                                           <  2:153337/141‑1 (MQ=255)
                                                                                                    aCGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTTCTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGAATTTAAAAGTACTGAAGtta                                         >  1:719806/1‑140 (MQ=255)
                                                                                                     cGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCAAAACAACCGGCTTTAAAACTACTAAAATTGa                                        >  2:25514/1‑141 (MQ=255)
                                                                                                        cTCCTTCAATCCCTGTGAATACCCATACAGTTACTGACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATa                                     <  1:651427/141‑1 (MQ=255)
                                                                                                        cTCCGTCAATCCCTGTGCATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATa                                     <  2:225306/141‑1 (MQ=255)
                                                                                                         tCCTTCAATCCCTGTAAATACCCATACAATTACTAACATAGTACTTTTTACTTGTGCCTTTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACCGAAGTCGATTAc                                    <  2:470050/141‑1 (MQ=255)
                                                                                                            ttCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACCCTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAAc                                 <  1:658453/141‑1 (MQ=255)
                                                                                                            ttCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTCTGCCATTGTATCTCCCCAACTCAAAACGCCACCACTTCCACTCGTCATACCATAAAAACCGGAGTTAAAAGTGCTCAACTCGAACACAAc                                 >  2:294426/1‑141 (MQ=255)
                                                                                                            ctCAATCCCTGTGAATACCCATACAGCTACTAACATAGTACTTTTTACTTGTGCCATTGTATCGCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGTATGTAAAAGTACTGAAGTTGAATACAAc                                 <  2:164741/140‑1 (MQ=255)
                                                                                                              cAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTa                               >  2:625894/1‑141 (MQ=255)
                                                                                                              cAATCCCTGTGAATAACAATCTGCGCACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTa                               >  2:738612/1‑141 (MQ=255)
                                                                                                               aaTCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTaa                              >  2:390481/1‑140 (MQ=255)
                                                                                                               aaTCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTAt                              <  1:584967/141‑1 (MQ=255)
                                                                                                               aaTCCCTGTGAACACCCATACAGTTACCAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACCAt                              <  2:666590/141‑1 (MQ=255)
                                                                                                                         aaTACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATAGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCAtata                    <  2:406633/141‑1 (MQ=255)
                                                                                                                          aTACCCATACAGTTACTGACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAACCCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATataa                   <  2:623667/141‑1 (MQ=255)
                                                                                                                          aTACCCATACAGTTACTGACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATataa                   <  2:456384/141‑1 (MQ=255)
                                                                                                                           tACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATataat                  <  2:189799/141‑1 (MQ=255)
                                                                                                                             cgcATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATAtaataa                <  2:438823/139‑1 (MQ=255)
                                                                                                                             cccATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATAtaataa                >  2:663848/1‑141 (MQ=255)
                                                                                                                             accagctaGTTGCTAACATAGTACTTTTTACTTGTGCCAGTGTATTGTCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATAtaataa                <  2:103409/134‑1 (MQ=255)
                                                                                                                               cATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACt              <  1:531597/141‑1 (MQ=255)
                                                                                                                               cATACAGTTACTAACATAGTACTTTCTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACt              <  2:73201/141‑1 (MQ=255)
                                                                                                                                  accattAATAATATAGTACTTTTTACTTTTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGa           <  2:149249/137‑1 (MQ=255)
                                                                                                                                   cAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGaa          <  1:371/141‑1 (MQ=255)
                                                                                                                                    aGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGaaa         <  1:122085/141‑1 (MQ=255)
                                                                                                                                       tACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGAAataa      >  2:98788/1‑140 (MQ=255)
                                                                                                                                           aaCATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGAAATATAGGt  >  1:737587/1‑141 (MQ=255)
                                                                                                                                           aaCATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGAAATATAGGt  <  1:381943/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAGTACAGTCATTAAGAAATAAATGACTAGCACAGAAATCAAACCAATAACGGTAGCAGTTCCTACATCCTTTTTAGACTTTGCACGTCCAGAAAAGACAACGGCTCCTTCAATCCCTGTGAATACCCATACAGTTACTAACATAGTACTTTTTACTTGTGCCATTGTATCTCCCCAACTAAAAACGCCAACACTTCCACTAGTCATACCATAAAAACCGGATTTAAAAGTACTGAAGTTGAATACAACTATCATGCATATAATAACTAGAAATATAGGT  >  USA300TCH1516_ALE/72212‑72491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑